Article
Chemistry, Analytical
Natasa Zurzul, Bjorn Torger Stokke
Summary: The study presents a potassium sensor based on DNA-aptamer functionalized hydrogel, capable of continuous label-free monitoring of potassium ions with high selectivity in an artificial blood buffer environment.
Article
Environmental Sciences
P. Salehan, Ali A. Ensafi, K. Zarean Mousaabadi, Jahan B. Ghasemi, E. Aghaee, B. Rezaei
Summary: An electrochemical aptasensor has been developed to determine K+ concentration using electrochemical impedance spectroscopy. The sensor utilizes polyaniline (PANI) coating and potassium-selective aptamer to detect K+. The presence of K+ leads to the formation of a G-quadruplex configuration, which hinders electron transfer. The sensor has a wide detection range and low detection limit, and can be applied to various sample types.
Article
Chemistry, Multidisciplinary
Tugce Ertan, Mustafa Oguzhan Caglayan
Summary: An ellipsometric biosensor was developed for detecting mercuric ions that affect vital organs, using nucleic acid-based aptamers with high selectivity. Two different types of aptamers were used, with detection sensitivities of 0.237 degrees/μM and 0.211 degrees/μM respectively. The detection limits for the linear and hairpin-type aptamers were 0.89 pM Hg2+ and 187 nM Hg2+ respectively.
Review
Food Science & Technology
Zanlin Chen, Miaojia Xie, Fengguang Zhao, Shuangyan Han
Summary: The increasing heavy metal pollution requires the development of a sensitive, simple, and reproducible detection method. Aptamers, as new artificial probes, have gained attention for their high sensitivity, easy acquisition, wide target range, and wide use in detecting harmful substances. The use of nanomaterials in aptamer-based electrochemical biosensors significantly improves the detection sensitivity.
Article
Chemistry, Multidisciplinary
Yin Bao, Kaiwei Cai, Yichen Liu, Bingling Li
Summary: The development of an accurate and practical electrochemical biosensor for the detection of kanamycin residues in water samples is described. The sensor demonstrates a simple construction method, good selectivity, and stability, and can provide rapid or real-time detection of kanamycin residues in water samples.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Applied
Jiafeng Pan, Zhi Liu, Junhua Chen
Summary: A biosensor for BPA detection was developed by integrating an amplifying DNA circuit with Mg2+-dependent DNAzyme in the sensing system, achieving a high sensitivity with a detection limit of 50 fM. The method proved to be selective and accurate, even working well in milk samples. The biosensor results were validated by LC-MS, showing no significant difference with the analytical method.
Article
Biophysics
Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Koichi Abe, Jinhee Lee, Kinuko Ueno, Ryutaro Asano, Brian Jones, Tomohiro Yamada, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Koji Sode, Toshimasa Toyo'oka, Kenichiro Todoroki, Kazunori Ikebukuro
Summary: A novel anti-idiotype aptamer A14#1 with extraordinary specificity against the anti-vascular endothelial growth factor therapeutic mAb, bevacizumab, was developed. The affinity of A14#1 to bevacizumab markedly increased at pH 4.7, enabling it to be potentially useful for therapeutic drug measurement as a novel ligand of bevacizumab.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Jeong Ah Park, Chaima Amri, Yein Kwon, Jin-Ho Lee, Taek Lee
Summary: Since 2010, DNA nanotechnology has made rapid advancements, providing new methods for constructing biosensors and overcoming the limitations of DNA solely as genetic material. The use of nucleic acids in biosensors has shown several advantages, and nanomaterials have also played a crucial role in advancing plasmonic biosensor technologies.
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Chin-Chung Tseng, Song-Yu Lu, Szu-Jui Chen, Ju-Ming Wang, Lung-Ming Fu, Yi-Hong Wu
Summary: An integrated microfluidic aptasensor device has been proposed for monitoring the concentration of potassium ions in the bloodstream of patients with chronic kidney disease. The device showed high sensitivity and convenience for point-of-care monitoring of whole blood potassium ion concentration.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Yan Guan, Peng-Bin Si, Tong Yang, Yuan Wu, Yun-Hui Yang, Rong Hu
Summary: A DNA walker-based dual-signal electrochemical ratiometric platform was developed for OTA detection, using Co-MOFs and toluidine blue as signal tag and reference probe. The method displayed superior sensitivity and reproducibility, with comparable results to commercial enzyme-linked immunoassay kits in determining OTA content in red wine samples.
Article
Chemistry, Multidisciplinary
Liangrui Lv, Juanjuan Hu, Qingqing Chen, Mingming Xu, Chunyang Jing, Xiaoying Wang
Summary: A switchable electrochemical hairpin-aptasensor has been established for the detection of OTA in food samples. The sensor utilizes a hairpin-DNA as a signal switch and combines gold nanospheres and multiwalled carbon nanotubes to establish a support interface. Conjugated polymer-nanoparticle composites are used to label report DNA, forming a signal probe. The sensor offers a low detection limit and a wide monitoring range.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hu Xing, Yiting Zhang, Markus Kraemer, Ann-Kathrin Kissmann, Valerie Amann, Heinz Fabian Raber, Tanja Weil, Kai R. Stieger, Uwe Knippschild, Marius Henkel, Jakob Andersson, Frank Rosenau
Summary: This article describes a FluCell-SELEX polyclonal aptamer library specific for R. intestinalis, which can be used for fluorescence microscopy and binding assays. The application of this library shows potential as an effective, rapid, and cost-effective method for quantifying R. intestinalis, providing a new tool for the diagnosis of gut microbiome-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Xiuxiu Yang, Xiaofang Zhao, Fengwei Liu, Haiyu Li, Claire Xi Zhang, Zhongqiang Yang
Summary: A new biosensor for detecting alpha-synuclein has been developed, utilizing the responsiveness of liquid crystals and specific interactions between a DNA aptamer and proteins. This biosensor shows rapid response time and high sensitivity, providing a promising approach for the simple and sensitive detection of alpha-synuclein.
Article
Chemistry, Analytical
Mary Quansah, Lisa Fetter, Autumn Fineran, Haley V. Colling, Keaton Silver, Teisha J. Rowland, Andrew J. Bonham
Summary: To overcome the challenges in early cancer detection, a new diagnostic tool called electrochemical DNA-based (E-DNA) biosensor has been developed. The biosensor can rapidly detect physiologically relevant levels of ENOX2, an attractive cancer target for blood tests. This design may lead to the development of more sensitive and rapid diagnostic tools for early cancer detection.
Article
Engineering, Environmental
Xiaochun Wang, Tiechun Li, Cuiping Ma
Summary: The novel fluorescent chemosensor MQS-Si shows highly sensitive detection of F- in solution, with a detection limit as low as 30 nM. Its application in selective detection in drinking water, urine, serum samples, as well as fluorescence imaging in living cells and zebrafish, demonstrates its potential utility in vitro and in vivo.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Xiaoli Zhao, Jian Zhang, Yake Duan, Qianyi Wan, Xin Zhang, Jiao Chen, Chao Shi, Yan Gao, Cuiping Ma
Summary: This study reports an accelerated strand exchange amplification (ASEA) method for ultra-fast, one-step RNA amplification detection of Salmonella in seafood. The ASEA method combines high sensitivity and specificity, and can complete the entire detection process in a short time.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Qi Li, Yang Li, Qian Gao, Chao Jiang, Qingwu Tian, Cuiping Ma, Chao Shi
Summary: Home-testing of SARS-CoV-2 is an ideal approach for controlling the COVID-19 pandemic and addressing the shortage of medical resources. A portable device was developed for real-time monitoring of isothermal nucleic acid amplification tests (INAATs) through the electrochemistry method. The device features a pH-sensitive potentiometric sensor for label-free detection of nucleic acid and a Bluetooth module for real-time monitoring on smartphones. The device demonstrated sensitivity and comparability with fluorescence and colorimetric assays.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Yong Li, Chunyu Yan, Jiao Chen, Yunyun Lian, Yingqiu Xie, Amr Amin, Qianyi Wan, Chao Shi, Cuiping Ma
Summary: This study developed an all-in-one platform for rapid and sensitive detection of Listeria monocytogenes. The platform integrates nucleic acid extraction, amplification, and visual detection in a single tube, and achieves high detection sensitivity using specific primers. Additionally, the method simplifies the use of detection equipment.
Article
Chemistry, Analytical
Yong Li, Xiangning Han, Xiaofeng Mu, Ye Wang, Chao Shi, Cuiping Ma
Summary: The false-negative result of nucleic acid testing is one of the important causes of COVID-19 transmission. This study developed a method using single-walled carbon nanotubes (SWCNTs) to protect and extract SARS-CoV-2 RNA, improving the sensitivity of RT-qPCR detection by 8-32 fold. The protection of RNA by SWCNTs allows for increased RNA load in downstream assays.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Multidisciplinary
Xiujuan Wang, Jiao Chen, Jinxiu Zhang, Yake Duan, Xin Zhang, Chao Shi, Yong Li, Cuiping Ma
Summary: This study developed a rapid and accurate method for detecting the pinewood nematode, with the entire detection process taking only 30 minutes. The method demonstrated high specificity and a low detection limit, successfully detecting the nematode in actual pinewood samples.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Chemistry, Analytical
Jinling Cui, Ritong Sun, Xiaoli Zhao, Mingyuan Zhao, Xiaojun Zhang, Yong Li, Lei Wang, Chao Shi, Cuiping Ma
Summary: In this study, an electrochemical DNA biosensor based on homogeneous hybridization in solution was designed for nucleic acid detection without probe immobilization. The magnetic biosensor constructed in this study could detect targets over a good linear dynamic range spanning 100 pM to 100 nM in 400 s, while those involving conventional hybridization methods always take 2 h or more. This strategy showed high specificity due to the specific binding of streptavidin and biotin. The homogenous hybridization magnetic biosensor constructed with electric field assistance presents a potential diagnostic method for rapid DNA detection and provides a new idea for rapid nucleic acid detection in clinical practice.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Jian Zhang, Xiangning Han, Ye Wang, Xiaofeng Mu, Chao Shi, Yong Li, Cuiping Ma
Summary: In this study, a staggered strand exchange amplification (SSEA) method was developed to detect Staphylococcus aureus with high specificity and efficiency at a constant temperature. The sensitivity of SSEA was 20 times that of the traditional method. An all-in-one SSEA platform was established by incorporating magnetic bead-based DNA extraction, which further enhanced the sensitivity of SSEA by two orders of magnitude. The SSEA platform showed high specificity and the ability to detect S. aureus in meat samples within 1 hour without a bacterial enrichment step, making it a promising tool for the food safety industry.
Article
Chemistry, Multidisciplinary
Jinling Guo, Yang Li, Qing Wang, Qianqian Jiang, Xinguang Zhang, Peng Zhang, Cuiping Ma, Chao Shi
Summary: Here, we developed a two-stage isothermal amplification method using strand exchange amplification and hyperbranched rolling circle amplification. This method only requires one DNA polymerase and can be performed in a single closed tube at a constant temperature, offering a promising signal amplification strategy for accurate and rapid pathogen detection in clinical settings.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Peng Zhang, Yang Li, Dongmei Zhang, Xinghao Zhu, Jinling Guo, Cuiping Ma, Chao Shi
Summary: RNA detection is crucial for controlling epidemics, and an ultrafast ligation-dependent RNA transcription amplification assay called SCAN is developed for real-time detection of SARS-CoV-2. The assay can be completed within 30-50 minutes and has a limit of detection of 10(4) copies per mL. The results show great agreement with commonly used detection methods, indicating its potential in clinical practice.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yong Li, Xin Zhang, Yu Liao, Chao Shi, Ye Wang, Xiaofeng Mu, Yingqiu Xie, Cuiping Ma
Summary: RNA-based detection of pathogenic organisms is a crucial field of research for disease diagnosis and environmental and food safety. Researchers have proposed a novel method for rapidly identifying long-stranded RNA using a rationally designed RNA-DNA structural template and RNase H reaction. This method is highly efficient, rapid, and flexible, making it a powerful tool for detecting long-stranded RNA and shedding light on pathogen detection in food safety and molecular diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Haiyan Fan, Kanat Dukenbayev, Qinglei Sun, Medina Khamijan, Akhrorbek Turdaliyev, Alibek Ysmaiyl, Aigerim Tassanbiyeva, Cuiping Ma, Yingqiu Xie
Summary: In this study, the addition of cauliflower-derived carbon dots was found to enhance alkaline phosphatase (ALP) activity, serving as a potential sensor for ALP. The combination of ALP and CFCDs exhibited typical Michaelis Menten kinetic behavior with improved kinetics and increased dephosphorylated product yield. Development of a CFCD-based paper strip assay allowed sensitive detection of elevated ALP levels and visualization of cancer stem cells or proteins.
Article
Chemistry, Multidisciplinary
Guldan Nazarbek, Aidana Kutzhanova, Lazzat Nurtay, Chenglin Mu, Bexultan Kazybay, Xugang Li, Cuiping Ma, Amr Amin, Yingqiu Xie
Summary: Researchers successfully predicted novel enzymes using a protein evolution analysis method, with experimental validation. Nanozymes are mainly classified as protein-based oxidoreductases, while peroxidase and lyase are the most conserved enzyme types in natural products. By simulating the evolution of natural protein enzymes, new natural nanozymes with lyase and peroxidase activities were discovered in Rhizoma polygonati and Goji herb extracts.
NANOSCALE ADVANCES
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)