Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Article
Biotechnology & Applied Microbiology
Lian-Ying Cao, Chen-Guang Liu, Shi-Hui Yang, Feng-Wu Bai
Summary: In this study, a novel strategy was developed to enhance the stress tolerance of Z. mobilis by regulating biofilm formation through heterologous expression of pfs and luxS genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Ecology
Tobias Sandfeld, Kirsten Gade Malmos, Camilla Bak Nielsen, Marie Braad Lund, Anne Aagaard, Jesper Bechsgaard, Martina Wurster, Michael Lalk, Mogens Johannsen, Thomas Vosegaard, Trine Bilde, Andreas Schramm
Summary: Animals in their natural habitats experience climatic variation, which can lead to phenotypic responses that differ among populations. Ectotherm arthropods may express thermoprotective metabolites in response to temperature stress, which help regulate cellular homeostasis and promote temperature tolerance. These metabolites can come from the animals themselves or their associated microbiomes, and microbial symbionts may play a role in shaping the thermal niche of their host. In this study, we investigated the metabolome and microbiomes of different populations of the social spider Stegodyphus dumicola, and found that the microbiomes showed no significant population-specific pattern, while the metabolome profiles differed significantly among populations and were linked to temperature stress and thermal tolerance.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Article
Cell Biology
Chandranayaka Siddaiah, Anil Kumar BM, Saligrama Adavigowda Deepak, Syed Salman Lateef, Saurabh Nagpal, Kanchugarakoppal S. Rangappa, Chakrabhavi D. Mohan, Shobith Rangappa, Madan S. Kumar, Minaxi Sharma, Vijai Kumar Gupta
Summary: This study focused on metabolite profiling and identification from the bark of Alangium salviifolium by using organic and aqueous solvents. Eighty-one secondary metabolites with therapeutic potential were identified, showcasing the therapeutic value of this plant.
Article
Chemistry, Applied
Mohamad Rafi, Alfi Hudatul Karomah, Rudi Heryanto, Dewi Anggraini Septaningsih, Wisnu Ananta Kusuma, Muhammad Bachri Amran, Abdul Rohman, Bambang Prajogo
Summary: Andrographis paniculata, known as the king of bitter, has been widely used as a medicinal plant. This research aims to identify the metabolite composition of stem and leaf extracts using LC-MS/MS and classify them using PCA. A total of 31 metabolite compounds were identified, which were divided into diterpene lactones, flavonoids, and phenolic acid groups. The extracts from leaves and stem were grouped based on the peak intensities of the identified compounds using PCA.
NATURAL PRODUCT RESEARCH
(2022)
Article
Agronomy
Ni Zhang, Weiwen Tan, Guimei Luo, Tianyi Pu, Jinqiu Wang, Xianhu Zhang, Yuehua Song
Summary: Camellia tetracocca, a unique tea species in southwest China, is frequently attacked by the pest Empoasca onukii. The metabolic mechanisms of C. tetracocca's defense against this pest are still unclear. Through comparative analysis using GC-MS and LC-MS, differential metabolites were identified, showing that accumulation of specialized metabolites may contribute to the resistance of C. tetracocca against E. onukii. These specialized metabolites have the potential to be used as biocontrol agents against the pest.
Article
Chemistry, Multidisciplinary
Anis Irfan Norazhar, Soo Yee Lee, Siti Munirah Mohd Faudzi, Khozirah Shaari
Summary: Christia vespertilionis is an ornamental plant with butterfly-shaped leaves, also known as butterfly wing or rerama in Malay. The green-leafed variety has gained popularity in Malaysia for its medicinal uses, yet there is limited information on its phytochemistry. Detailed analysis revealed a variety of metabolites in the leaf, with flavonoids being the major class. Further research is needed for the potential valorization of this plant as a phytopharmaceutical product.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Nina Sipari, Jenna Lihavainen, Markku Keinanen
Summary: RADICAL-INDUCED CELL DEATH1 (RCD1) is a nuclear protein in Arabidopsis thaliana, involved in development and stress responses. Rcd1 plants show resistance to the herbicide paraquat, possibly through elevated levels of antioxidants and specialized metabolites. The study suggests that RCD1 acts as a hub to maintain the basal antioxidant system and induces defense responses to oxidative stress.
Article
Biochemistry & Molecular Biology
Yasuko Fujisawa, Yohei Masunaga, Wataru Tanikawa, Shinichi Nakashima, Daisuke Ueda, Shinichiro Sano, Maki Fukami, Hirotomo Saitsu, Takashi Yazawa, Tsutomu Ogata
Summary: This study investigated the symptoms and genetic mutations of two Japanese patients with 17-beta hydroxysteroid dehydrogenase type 3 (HSD17B3) deficiency. The study found that these patients had higher T/A-dione ratios compared to normal males, but the ratios were lower than those in the control group, likely due to residual HSD17B3 function.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Analytical
You-Rim Lee, Seungyeon Lee, Sohyen Kwon, Jiyeong Lee, Hee-Gyoo Kang
Summary: Metabolomic research has been conducted in various fields, but the combination of forensic science and metabolomics is rare. This study identified and validated metabolite markers for determining the age of bloodstains within a week post-bleeding. These markers could be used as innovative tools for reconstructing crime scenes and suggesting initial investigative direction.
ANALYTICAL CHEMISTRY
(2022)
Article
Horticulture
Jung-Sung Chung, Hyeong-Cheol Kim, Su-Min Yun, Hyun-Jin Kim, Cheol-Soo Kim, Jeung-Joo Lee
Summary: Sulfur is an essential nutrient for plant growth and metabolism, and significantly affects the growth and metabolites of lettuce. Higher sulfur concentration treatment resulted in increased growth and metabolite content in lettuce compared to lower sulfur concentration treatment.
Article
Pharmacology & Pharmacy
Ermin Rachmawati, Suharti Suharti, Djanggan Sargowo, Larasati Sekar Kinasih, Yudi Her Octaviano, Roihatul Mutiah, Mahrus Ismail, Ahmad Munjin Nasih
Summary: This study aimed to investigate the secondary metabolites, hypolipidemic, and anti-atherogenic effect of mixed vegetable fermentation extract (MVFE). Seventeen secondary metabolites were identified using LC-MS/MS, and it was found that MVFE exerted its athero-protective effect by modulating various cellular processes such as inflammation, endothelial function, and lipid metabolism. Animal experiments confirmed that MVFE dose-dependently reduced total cholesterol and LDL-c levels.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
Article
Food Science & Technology
Asmaa M. Otify, Shahira A. ElBanna, Basma M. Eltanany, Laura Pont, Fernando Benavente, Rana M. Ibrahim
Summary: This study characterized the metabolite profiles of six lettuce varieties and established their correlation with antivirulence effects. Various metabolites were identified, and red varieties showed higher levels of chlorogenic and chicoric acids indicating antioxidant properties. Romaine longifolia exhibited enrichment in amino acids and disaccharides, while crispa, capitata, and lolla bionda had a high content of β-carboline alkaloids. All varieties demonstrated antibacterial potential against Staphylococcus aureus strains, and specific bioactives such as sesquiterpenes, β-carboline alkaloids, amino acids, and oxy-fatty acids were identified. The results highlight the significance of lettuce as a functional food source and suggest breeding programs should focus on varieties rich in antibacterial agents.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Manamele Dannies Mashabela, Fidele Tugizimana, Paul Anton Steenkamp, Lizelle Ann Piater, Ian Augustus Dubery, Msizi Innocent Mhlongo
Summary: This study used LC-MS technology to analyze the metabolomic responses of Pst-resistant and susceptible wheat varieties to P. striiformis infection. The results showed that wheat plants undergo time-dependent metabolic reprogramming in response to the pathogen, with changes in phenylpropanoids, amino acids, lipids, benzoic acids, TCA cycle intermediates, and benzoxazinoids. The resistant cultivar Koonap accumulated defense metabolites more rapidly than the susceptible Gariep cultivar, suggesting the potential for metabolic engineering to improve plant tolerance and resistance to biotic stresses.
Article
Biochemistry & Molecular Biology
Qing Yi-Jun Zhou, Xin Liao, Hao-Ming Kuang, Jia-Yu Li, Shui-Han Zhang
Summary: This study systematically identified the metabolites in extracts from the roots, leaves, twigs, and fruits of the Morus alba tree, and evaluated their hypoglycemic activity. The extracts from the roots and twigs showed significant hypoglycemic activity, and a high-level component called morusin was found to decrease blood glucose levels. This research provides a basis for further development of Morus alba extracts as anti-diabetic drugs.
Article
Pharmacology & Pharmacy
Wanshan Feng, Chaolong Qin, Salah Abdelrazig, Ziyu Bai, Mekha Raji, Randa Darwish, YenJu Chu, Liuhang Ji, David A. Gray, Michael J. Stocks, Cris S. Constantinescu, David A. Barrett, Peter M. Fischer, Pavel Gershkovich
Summary: This study investigated the composition differences of different vegetable oils and their effects on promoting CBD lymphatic targeting and bioavailability. The results showed that olive oil led to the highest concentration of CBD in the lymphatic system and in the systemic circulation following oral administration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Valentina Cuzzucoli Crucitti, Aleksandar Ilchev, Jonathan C. Moore, Harriet R. Fowler, Jean-Frederic Dubern, Olutoba Sanni, Xuan Xue, Bethany K. Husband, Adam A. Dundas, Sean Smith, Joni L. Wildman, Vincenzo Taresco, Paul Williams, Morgan R. Alexander, Steven M. Howdle, Ricky D. Wildman, Robert A. Stockman, Derek J. Irvine
Summary: This study utilized the alpha parameter, a molecular descriptor, to design terpene-based polymers resistant to biofilm formation by Pseudomonas aeruginosa. The potential biofilm resistance of terpene-derived monomers was predicted and ranked using the alpha parameter. The monomers from commercially available terpenes were polymerized and confirmed to have biofilm resistance properties. The scaled-up monomers were successfully printed and used to create bio-instructive microparticles. The observations in this study have significant potential to produce bio-resistant coatings, packaging materials, fibers, and medical devices.
Article
Microbiology
Jonathan R. R. Humphreys, Bisrat J. J. Debebe, Stephen P. P. Diggle, Klaus Winzer
Summary: Solventogenic clostridia are anaerobic, spore-forming bacteria that produce acetone, butanol, and ethanol through biphasic metabolism. However, these bacteria have a tendency to degenerate, losing their ability to produce solvents and spores, which is a problem for industrial applications. Through comparative genomics and ultra-deep sequencing, researchers identified the spo0A gene as key to the degeneration process in Clostridium beijerinckii NCIMB 8052. Mutations in this gene and genes regulating its expression and activity were found in hotspot regions of degenerate variants. This study provides new insights into clostridial strain degeneration and spore regulation network.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jean-Frederic Dubern, Andrew L. Hook, Alessandro M. Carabelli, Chien-Yi Chang, Christopher A. Lewis-Lloyd, Jeni C. Luckett, Laurence Burroughs, Adam A. Dundas, David J. Humes, Derek J. Irvine, Morgan R. Alexander, Paul Williams
Summary: Innovative approaches are urgently needed to prevent catheter-associated urinary tract infections (CAUTIs). A newly discovered acrylate copolymer has shown promising resistance against bacterial biofilm formation, swarming, encrustation, and host protein deposition, all of which are major challenges in preventing CAUTIs. The copolymer, poly(tert-butyl cyclohexyl acrylate), combined with poly(2-hydroxy-3-phenoxypropyl acrylate) has demonstrated its potential as a urinary catheter coating by retaining its bioinstructive properties and maintaining resistance against various uropathogens.
Article
Nanoscience & Nanotechnology
Leonardo Contreas, Andrew L. Hook, David A. Winkler, Grazziela Figueredo, Paul Williams, Charles A. Laughton, Morgan R. Alexander, Philip M. Williams
Summary: Bacterial infections are becoming a greater concern due to antimicrobial resistance. The rational design of materials resistant to biofilm formation is a crucial strategy to prevent infections related to medical devices. Machine learning has been used to identify strong associations between bacterial adhesion and the physicochemical properties of polyacrylate libraries. However, the interpretability of nonlinear models is limited, making it challenging to understand the molecular interactions between materials and bacteria.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biophysics
Heba Khateb, Andrew L. Hook, Stefanie Kern, Julie A. Watts, Sonali Singh, Darryl Jackson, Luisa Marinez-Pomares, Paul Williams, Morgan R. Alexander
Summary: The use of OrbiTrap analyzer with SIMS (3D OrbiSIMS) enables label-free mass spectrometry of Psl, accurately identifying secondary ion peaks specific to the Psl structure. By studying P. aeruginosa biofilm and purified Psl, 17 peaks associated with Psl fragments within the biofilm matrix were confidently identified. Additionally, the neutral loss sequences approach can also identify multiple oligosaccharide fragments without a biological reference sample.
Article
Microbiology
Jean-Frederic Dubern, Nigel Halliday, Miguel Camara, Klaus Winzer, David A. Barrett, Kim R. Hardie, Paul Williams
Summary: Quorum sensing (QS) in Pseudomonas aeruginosa relies on the Las, Rhl, and Pqs systems to regulate the synthesis of AHLs and AQs. While QS is commonly thought to be population density-dependent, this study demonstrates that growth rate and population density independently modulate the accumulation of AHLs and AQs. Different nutrient limitations, temperature, and mutation of key QS genes also affect the production of AHLs and AQs in P. aeruginosa. QS imposes an energetic drain on the bacterium by consuming key substrates and ATP.
Article
Multidisciplinary Sciences
Alessandra Ottonello, Jessica A. Wyllie, Oussama Yahiaoui, Shoujun Sun, Rebecca A. Koelln, Joshua A. Homer, Robert M. Johnson, Ewan Murray, Paul Williams, Jani R. Bolla, Carol Robinson, Thomas Fallon, Tatiana P. Soares da Costa, John E. Moses
Summary: The rise of drug-resistant superbugs poses a significant global health hazard. A new class of shapeshifting vancomycin dimers has been synthesized, which shows potent activity against drug-resistant bacteria. These shapeshifting antibiotics have the ability to inhibit bacterial cell wall biosynthesis and are not easily affected by common resistance mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Giulia Giallonardi, Morgana Letizia, Marta Mellini, Emanuela Frangipani, Nigel Halliday, Stephan Heeb, Miguel Camara, Paolo Visca, Francesco Imperi, Livia Leoni, Paul Williams, Giordano Rampioni
Summary: Pseudomonas aeruginosa is a model pathogen with multiple quorum sensing circuits that control virulence factor production and biofilm formation. The pqs QS system in P. aeruginosa synthesizes AQs including HHQ and PQS, which act as signal molecules. Transcriptomic analyses show that HHQ and PQS influence gene expression through PqsR-dependent and -independent pathways. However, HQNO has no effect on P. aeruginosa transcriptome. Mutation of pqsL in P. aeruginosa leads to autolysis in colony biofilms through the accumulation of HHQ and activation of Pf4 prophage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
See Yoong Wong, Andrew L. Hook, Wil Gardner, Chien-Yi Chang, Ying Mei, Martyn C. Davies, Paul Williams, Morgan R. Alexander, Davide Ballabio, Benjamin W. Muir, David A. Winkler, Paul J. Pigram
Summary: Biofilm formation is a major problem in hospitals, and researching biofilm-resistant materials is critical. Polymer microarrays can efficiently discover new biofilm-resistant polymers. This study investigates bacterial attachment and surface chemistry on a polymer microarray to better understand Pseudomonas aeruginosa biofilm formation. Analyzing the data using linear multivariate analysis and a nonlinear self-organizing map reveals fragment ions associated with bacterial biofilm formation. Considering these insights, a second analysis is conducted that explicitly considers interactions between key fragments. This improved model provides chemical insights for designing materials that prevent pathogen attachment.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Philip Bardelang, Ewan J. Murray, Isobel Blower, Sara Zandomeneghi, Alice Goode, Rohanah Hussain, Divya Kumari, Giuliano Siligardi, Katsuaki Inoue, Jeni Luckett, James Doutch, Jonas Emsley, Weng C. Chan, Philip Hill, Paul Williams, Boyan B. Bonev
Summary: The virulence gene expression in S. aureus is controlled by the agr quorum sensing system, and the AgrB and AgrD proteins play important roles in this process. Through homology modelling and molecular dynamics techniques, the conformations of AgrB and AgrD were characterized and their interaction mechanisms were analyzed. Experimental evidence confirmed the direct interaction between AgrB and AgrD, and the formation of AgrBD complex in vitro. These findings contribute to a better understanding of the mechanisms behind the regulation of virulence gene expression in S. aureus.
FRONTIERS IN CHEMISTRY
(2023)
Article
Microbiology
Jonathan R. Humphreys, Zak Bean, Jamie Twycross, Klaus Winzer
Summary: Lanthipeptide synthetases, present in all domains of life, catalyze an important step in lanthipeptide biosynthesis. LanM-like proteins, found in Clostridium species, are associated with agr quorum sensing operons. The physiological role and mode of action of these proteins are unclear. In this study, the LanM-like protein CA_C0082 was shown to act as an effector of Agr quorum sensing, controlling granulose formation and spore and solvent production in Clostridium acetobutylicum.
Article
Biochemistry & Molecular Biology
Macauley J. Green, Ewan J. Murray, Paul Williams, Amir M. Ghaemmaghami, Jonathan W. Aylott, Philip M. Williams
Summary: Under low-shear modeled microgravity conditions, Staphylococcus aureus exhibited a colonization phenotype instead of a pathogenic one, due to reduced production of autoinducing peptide signal molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Marta Mellini, Morgana Letizia, Lorenzo Caruso, Alessandra Guiducci, Carlo Meneghini, Stephan Heeb, Paul Williams, Miguel Camara, Paolo Visca, Francesco Imperi, Livia Leoni, Giordano Rampioni
Summary: Quorum sensing allows single cells to synchronize gene expression in bacterial populations, but studies have shown significant cell-to-cell variation in this process, resulting in coexisting subpopulations of active and inactive cells. The molecular mechanisms behind this phenomenon, particularly in the las QS system of Pseudomonas aeruginosa, have not been fully defined.
Article
Chemistry, Analytical
Malak A. Jaber, Bruna de Falco, Salah Abdelrazig, Catharine A. Ortori, David A. Barrett, Dong-Hyun Kim
Summary: In comprehensive lipidomics studies, accurate quantification is hindered by unwanted variations such as lipid degradation, matrix effects, and non-linear responses of instruments. This study employed an in vivo labelling strategy to generate C-13-labelled internal standards (C-13-ISs) from different species for more accurate and quantitative lipidomics analysis. The use of C-13-labelled P. pastoris extract as internal standards resulted in significant reduction in lipid variations compared to other normalization methods. This study highlights the benefit of in vivo labelling strategy in reducing technical and analytical variations in lipidomics studies.
ANALYTICAL METHODS
(2023)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
