Review
Biochemical Research Methods
Elizabeth C. Wilkirson, Kavya L. Singampalli, Jiran Li, Desh Deepak Dixit, Xue Jiang, Diego H. Gonzalez, Peter B. Lillehoj
Summary: The detection and quantification of biomarkers in blood is crucial for the early identification, diagnosis, and treatment of various diseases. Affinity-based electrochemical sensors, which offer high sensitivity, specificity, fast detection times, ease of use, and portability, are a promising approach for detecting molecular biomarkers. However, the complexity of whole blood poses challenges, requiring sample purification and sophisticated equipment. This article provides an overview of strategies employed to improve the performance of affinity-based electrochemical sensors for biomolecular detection in whole blood.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Nanoscience & Nanotechnology
Kun Chen, Ming Jiang, Jin Liu, Deli Huang, Yuhe R. R. Yang
Summary: Nanoparticle-based vaccines offer a multivalent approach for antigen display, efficiently activating T and B cells in the lymph nodes. DNA nanotechnology provides an innovative alternative platform, featuring high modularity, spatial addressing, nanoscale regulation, high functional group density, and lower self-antigenicity. This review explores the potential of DNA nanostructures as biomolecular scaffolds for antigen display, discussing immunological mechanisms, characterization techniques, advancements in DNA nanotechnology, strategies for precise antigen presentation, and current applications and future possibilities.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Review
Chemistry, Analytical
Rohini Kumari, Daphika S. S. Dkhar, Supratim Mahapatra, Divya, Surinder P. P. Singh, Pranjal Chandra
Summary: Metallic dendrites, with their unique hierarchical structures and large active surface areas, have attracted significant attention. Due to their high catalytic activity and conductivity, metallic dendrites are increasingly used in sensor fabrication. This review summarizes various nanodendrite-engineered biosensors for monitoring different substances in real samples.
Article
Chemistry, Analytical
Lara F. Loguercio, Anderson Thesing, Pedro Demingos, Carlos D. L. de Albuquerque, Roberta S. B. Rodrigues, Alexandre G. Brolo, Jacqueline F. L. Santos
Summary: Efficient and portable sensors are needed for monitoring pesticide contaminants in various environments. This study focused on a biocomposite film, which optimized surface conditions for enzyme immobilization and showed improved catalytic performance. The biosensor demonstrated linear responses for carbaryl detection with good repeatability and reproducibility, and improved stability at lower temperatures.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Ian Ivar Suni
Summary: Electrochemical biosensors have potential applications in various fields, but one of the primary challenges lies in immobilizing biomolecular probes onto solid substrate materials with adequate stability and reproducibility. Recent research has focused on transition metal dichalcogenides and electrically conductive polymers as new materials for covalent bonding of biomolecules.
Article
Biotechnology & Applied Microbiology
Tian Jiang, Chenyi Li, Yusong Zou, Jianli Zhang, Qi Gan, Yajun Yan
Summary: In this study, an AutoCAD regulation system was developed to automatically coordinate pathway expression for enhanced naringenin production in microbial cells. The system, consisting of intermediate-based feedforward and product-based feedback control genetic circuits, significantly increased naringenin titer. This approach provides a new paradigm for developing dynamic control strategies to engineer heterologous pathways.
METABOLIC ENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Hironaga Noguchi, Yoshiki Nakamura, Sayaka Tezuka, Takakazu Seki, Kazuki Yatsu, Takuma Narimatsu, Yasuaki Nakata, Yuhei Hayamizu
Summary: Biosensors with two-dimensional materials have gained wide interest due to their high sensitivity. Among them, single-layer MoS2 has become a new class of biosensing platform owing to its semiconducting property. Immobilization of bioprobes directly onto the MoS2 surface with chemical bonding or random physisorption has been widely studied. However, these approaches potentially cause a reduction of conductivity and sensitivity of the biosensor.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Analytical
Corwin A. Miller, Joanne M. L. Ho, Matthew R. Bennett
Summary: This article reviews the known types of mechanisms of biosensors within bacterial cells and discusses different approaches for optimizing biosensor functional parameters, including gene engineering, selection of genetic reporters, gene expression tuning, and incorporation of additional genetic modules.
Article
Chemistry, Analytical
Jun Kurosu, Kaname Kanai, Jun'ya Tsutsumi
Summary: We developed a label-free and highly surface-sensitive bioimaging technique, named as electric-double-layer modulation imaging (EDLMI). This technique utilized the charge density of electric double layer, accumulated at the interface between a semiconductor and electrolyte solution, to sensitively respond to bio-molecular adsorption. By utilizing lock-in imaging in synchronization with electric-double-layer modulation, EDLMI can monitor nano-thick biomolecular adsorption in real time with a wide-range magnification, distinguishing specific and non-specific binding between antibodies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Optics
Lei Han, Chaoyu Xu, Tianye Huang, Xueyan Dang
Summary: This paper introduces the improved particle swarm optimization algorithm for optimizing the design of surface plasmon resonance sensors, achieving the best structure and improving sensitivity and figure of merit.
Article
Electrochemistry
Ayaz Hassan, Lucyano J. A. Macedo, Isabela A. Mattioli, Rafael J. G. Rubira, Carlos J. L. Constantino, Rodrigo G. Amorim, Filipe C. D. A. Lima, Frank N. Crespilho
Summary: The study introduces a three-component vertically designed monolayer graphene device that exhibits higher chemical reactivity and sensitivity in biochemical sensing. Gold layer transferring electronic density to graphene surface effectively enhances the charge transfer rate with ferrocene. The technology demonstrates high sensitivity and low detection limit in DNA hybridization detection.
ELECTROCHIMICA ACTA
(2021)
Article
Engineering, Environmental
Xiang Qi, Shuyi Wang, Yong Jiang, Panpan Liu, Qingcheng Li, Wen Hao, Jinbin Han, Yuexi Zhou, Xia Huang, Peng Liang
Summary: This study successfully prepared an EAB biosensor using sodium alginate as an immobilization agent, which could generate highly consistent electrical signals in less than an hour with high sensitivity. The research showed that the appropriate concentrations of bacteria and alginate would affect the performance of the biosensor.
Article
Chemistry, Physical
Lokesh K. Gangwar, Vikash Sharma, Amit Choudhary, Gajjala Sumana, S. S. Pandey, Hirofumi Tanaka, Ashok M. Biradar, Rajesh
Summary: A dielectric biosensor based on a nematic liquid crystal (LC) platform was developed for the detection of human cardiac troponin I (cTnI). The optical and dielectric responses of the biosensor were studied as a function of cTnI concentration. Gold nanoparticles conjugated with detection antibody were used to enhance the signal of the biosensor. The binding of AB-GNP with cTnI caused disturbances in LC alignment and a change in transmission. The dielectric response of the biosensor was fitted with a Hill-Langmuir equation, revealing strong binding affinity between cTnI and the substrate antibody.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Jihyun Kim, J. Tassilo Grun, Mihajlo Novakovic, Eriks Kupce, Rina Rosenzweig, Lucio Frydman
Summary: This paper discusses a new cross polarization (CP) strategy that combines frequency-swept and phase-modulated pulses to improve the efficiency of N-15 transfers. Theoretical analysis and experimental results demonstrate that this new strategy outperforms current options on urea, amino acids, and intrinsically disordered proteins.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Abigail Barclay, Birthe B. Kragelund, Lise Arleth, Martin Cramer Pedersen
Summary: Recent advances in protein expression protocols have allowed for the investigation of structurally complex and disordered biomolecules using small-angle scattering experiments. A modeling scheme has been developed that combines classical form factor based modeling with spherical harmonics-based formulation to accurately calculate scattering profiles from these complex samples. The scheme can account for flexible domains and other structurally elaborate components, and we demonstrate its utility through a case study on a growth hormone receptor membrane protein. We also explore how the scattering profiles vary under different contrasts and discuss the implications for data modeling.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jakub Zbigniew Kaczmarek, Peter Durand Skottrup
MOLECULAR IMMUNOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Rasmus P. Clausen, Andreas O. Mohr, Erik Riise, Anders A. Jensen, Avinash Gill, Dean R. Madden, Jette S. Kastrup, Peter D. Skottrup
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2016)
Article
Biochemical Research Methods
Peter Durand Skottrup
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2017)
Article
Dentistry, Oral Surgery & Medicine
Peter D. Skottrup, Gunnar Dahlen, Vibeke Baelum, Rodrigo Lopez
EUROPEAN JOURNAL OF ORAL SCIENCES
(2018)
Article
Engineering, Biomedical
Peter Durand Skottrup, Mikkel Fougt Hansen, Jacob Moresco Lange, Maria Deryabina, Winnie Edith Svendsen, Mogens Havsteen Jakobsen, Martin Dufva
ACTA BIOMATERIALIA
(2010)
Article
Biochemical Research Methods
Tibisay Guevara, Miroslaw Ksiazek, Peter Durand Skottrup, Nuria Cerda-Costa, Sergio Trillo-Muyo, Inaki de Diego, Erik Riise, Jan Potempa, F. Xavier Gomisrueth
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2013)
Article
Biochemical Research Methods
Peter Durand Skottrup, Paul Leonard, Jakub Zbigniew Kaczmarek, Florian Veillard, Jan Johannes Enghild, Richard O'Kennedy, Aneta Sroka, Rasmus Praetorius Clausen, Jan Potempa, Erik Riise
ANALYTICAL BIOCHEMISTRY
(2011)
Review
Biophysics
Peter Durand Skottrup, Mogens Nicolaisen, Annemarie Fejer Justesen
BIOSENSORS & BIOELECTRONICS
(2008)
Article
Infectious Diseases
A. Knudsen, U. S. Kristoffersen, I. Panum, Y. B. Hansen, P. D. Skottrup, P. Hasbak, A. Kjaer, A-M Lebech
Article
Multidisciplinary Sciences
Peter Durand Skottrup, Grete Sorensen, Miroslaw Ksiazek, Jan Potempa, Erik Riise
Article
Biochemistry & Molecular Biology
Gustavo G. Nascimento, Vibeke Baelum, Timo Sorsa, Taina Tervahartiala, Peter D. Skottrup, Rodrigo Lopez
Article
Biochemical Research Methods
Peter Durand Skottrup, Rodrigo Lopez, Miroslaw Ksiazek, Peter Hojrup, Vibeke Baelum, Jan Potempa, Jakub Zbigniew Kaczmarek
JOURNAL OF IMMUNOLOGICAL METHODS
(2019)
Article
Anthropology
Peter Durand Skottrup, Thomas Kallemose, Diana Espino, Rocio Infante-Ramirez, Soren Brage, Dijana Terzic, Jens Peter Goetze, Jesper Kjaergaard, Dirk Lund Christensen
Summary: Plasma suPAR concentrations significantly increased in Mexican Tarahumara men and women following ultra-distance running, and remained elevated for up to 6 hours post-race regardless of running intensity. The results suggest that suPAR can complement, but not replace CRP as a systemic inflammation biomarker in this population.
AMERICAN JOURNAL OF HUMAN BIOLOGY
(2021)
Article
Biochemical Research Methods
Peter Skottrup, Mogens Nicolaisen, Annemarie F. Justesen
JOURNAL OF MICROBIOLOGICAL METHODS
(2007)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
