Article
Chemistry, Inorganic & Nuclear
Ling Gao, Luyao Chen, Yu Peng, Yibo Zhao, Jianhua Dong, Ziwang Mao, Junli Jia, Yuyang Zhou
Summary: In this study, three iridium(iii) tetrazolato complexes were designed and synthesized, and their performance in protein staining was comprehensively investigated. These complexes exhibited better protein staining properties compared to the commercial agent Coomassie Brilliant Blue (CBB), paving the way for exploring new efficient iridium-based protein staining agents in both commercial markets and academic research in the future.
DALTON TRANSACTIONS
(2022)
Article
Materials Science, Multidisciplinary
J. Ebad-Allah, S. Rojewski, Y. L. Zhu, Z. Q. Mae, C. A. Kuntscher
Summary: Polarization-dependent reflectivity measurements were conducted on ZrGeSe and ZrGeS compounds, showing their strong anisotropic character and changes in conductivity spectrum under external pressure.
Article
Chemistry, Physical
Aranyak Sarkar, Vinu Namboodiri, Manoj Kumbhakar
Summary: This article investigates the molecular interaction and local environment of an amyloid fibril sensor by using fluorescence readouts. The study focuses on the organization of fibril nanostructures and the binding configurations of the sensor. The observation of both in-plane and out-of-plane binding modes emphasizes the important role of the electron donor amino group in fluorescence detection.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemical Research Methods
Yui Tomioka, Masataka Nakagawa, Chiaki Sakuma, Yasunori Kurosawa, Satoru Nagatoishi, Kouhei Tsumoto, Tsutomu Arakawa, Teruo Akuta
Summary: This study attempted to stain unfolded proteins specifically on agarose native gels. It was found that SYPRO Orange dye barely bound to native proteins but effectively stained unfolded proteins. The dye also stained protein bands in the presence of SDS, indicating the presence of an intermediate unfolded structure.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Physics, Multidisciplinary
Wan Xu, Victor Naden Robinson, Xiao Zhang, Hui-Chao Zhang, Mary-Ellen Donnelly, Philip Dalladay-Simpson, Andreas Hermann, Xiao-Di Liu, Eugene Gregoryanz
Summary: Mixtures of ammonia and water are key components in the hot ice mantle regions of icy planets, with AHH playing a pivotal role in precipitating from water-rich mixtures under pressure. Spectroscopic measurements show the spontaneous ionization of AHH under compression, transforming from molecular to ionic states.
PHYSICAL REVIEW LETTERS
(2021)
Article
Physics, Multidisciplinary
J. Ebad-Allah, S. Rojewski, M. Voest, G. Eickerling, W. Scherer, E. Uykur, Raman Sankar, L. Varrassi, C. Franchini, K. -H. Ahn, J. Kunes, C. A. Kuntscher
Summary: The anisotropic optical response of the layered, nodal-line semimetal ZrSiS under different pressures was investigated, showing strong pressure-induced effects on the out-of-plane optical conductivity spectrum. These effects cannot be explained by structural phase transitions or electronic correlations, and are related to the excitonic insulator phase proposed in ZrSiS.
PHYSICAL REVIEW LETTERS
(2021)
Article
Microbiology
Jianhua Zhang, Alfons J. M. Debets, Paul E. Verweij, Sijmen E. Schoustra
Summary: The Flamingo Medium was found to be the most effective in selectively isolating Aspergillus fumigatus from environmental samples, inhibiting the growth of competing fungi and recovering more A. fumigatus colonies compared to other media. It can also inhibit the growth of clinical Mucorales, making it a valuable tool for both environmental and clinical studies.
Article
Chemistry, Multidisciplinary
Tetsuya Yamada, Takanori Watanabe, Kazuaki Hatsusaka, Jianjun Yuan, Michihisa Koyama, Katsuya Teshima
Summary: The flux method is an efficient technique for liquid-phase crystal growth, but it is challenging to establish guidelines due to the complexity of the crystal growth mechanism. In this study, the influence of raw material information as explanatory variables on the prediction of flux crystal growth was investigated. The findings provide new insights into the PI-assisted flux method, contributing to the development of highly accurate prediction models.
Article
Chemistry, Multidisciplinary
Piotr A. Gunka, Michael Hanfland, Yu-Sheng Chen, Janusz Zachara
Summary: High-pressure structural studies of arsenic(III) oxide polymorphs reveal that claudetite I is the densest and least compressible ambient-pressure As2O3 polymorph, with no phase transitions under high pressure or low temperature. The coordination sphere of arsenic atoms is thoroughly characterized, showing that claudetite I does not exhibit the highest coordination numbers despite being the densest polymorph. The pressure dependence of As-O bond lengths and stereoactivity of arsenic lone electron pairs are also investigated, demonstrating consistent behaviors across different As2O3 polymorphs.
Article
Biochemistry & Molecular Biology
Yui Tomioka, Satoru Nagatoishi, Masataka Nakagawa, Kouhei Tsumoto, Tsutomu Arakawa, Teruo Akuta
Summary: The Ferguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) during thermal unfolding. Different factors such as pH, protein concentration, and buffer type influenced the formation of these intermediate species. By analyzing the size and staining properties of the intermediate species, it was determined that some species were native or native-like in structure, while others had unfolded structures and larger hydrodynamic size compared to native BSA.
BIOPHYSICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Aruna K. Mora, Sukhendu Nath
Summary: A new zwitterionic merocyanine dye, SYPRO Orange, has been shown to detect amyloid fibrils more sensitively than the current gold standard Thioflavin T. Detailed photophysical studies reveal the mechanism behind its responsiveness to amyloid fibrils in different solvents and solvent mixtures.
Article
Multidisciplinary Sciences
Hao Wang, Jiaxin Yang, Yu Zhang, Jianliang Qian, Jianrong Wang
Summary: The team introduces a computational method called FLAMINGO, which can generate high-resolution 3D chromosomal structures for all chromosomes in the human genome across multiple cell-types with superior accuracy and scalability compared to other methods.
NATURE COMMUNICATIONS
(2022)
Review
Mineralogy
Wasura Soonthorntantikul, Ungkhana Atikarnsakul, Wim Vertriest
Summary: Burmese sapphires are highly sought after for their historical significance and fine quality, showing a wide range of blue color intensities and consistent inclusion scenes. Differences in iron concentrations can be observed, with rare occurrences such as orange fluorescence and abnormal FTIR spectra attributed to the chemical compositions of the sapphires.
Article
Biology
Jorge S. Gutierrez, Johnnie N. Moore, J. Patrick Donnelly, Cristina Dorador, Juan G. Navedo, Nathan R. Senner
Summary: This study explores the impact of climate change and lithium mining on water availability and flamingo abundance in the Chilean Andes. The results show that both factors can have negative effects, with mining negatively correlated with flamingo abundance in the mining area. Efforts to mitigate mining expansion and climate change are urgently needed to protect local biodiversity and the local economy.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Biology
Hiroki Takechi, Satoko Hakeda-Suzuki, Yohei Nitta, Yuichi Ishiwata, Riku Iwanaga, Makoto Sato, Atsushi Sugie, Takashi Suzuki
Summary: The study shows that insulin secreted from glia regulates the function of the transmembrane protein Gogo, influencing the development of R8 photoreceptor axons in the Drosophila visual system and ultimately shaping the organization of the entire visual system.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)