期刊
ANALYTICAL BIOCHEMISTRY
卷 396, 期 2, 页码 304-309出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.10.004
关键词
DNA biosensor; Chronocoulometry; Poly(L-lysine); Gold nanoparticles; Electropolymerization
资金
- National Nature Science Foundation of China [20675002]
In this work, we fabricated a sensitivity chronocoulometric DNA sensor (CDS) based on gold nanoparticles (AuNPs)/poly(L-lysine) complex film modified glassy carbon electrode. Hexaammineruthenium(III) chloride ([Ru(NH3)(6)](3+)) was used as the electroactive indicator. The assembled process was investigated by cyclic voltammetry (CV) and chronocoulometry, (CC). CC is used to monitor the DNA hybridization event by measurement of electrostatic binding [Ru(NH3)(6)](3+). Under the optimal conditions, the signal of [Ru(NH3)(6)](3+). Was linear with the logarithm of the concentration of the complementary oligonucleoticles from 1.0 x 10(-13) to 1.0 X 10(-11) M, and the detection limit is 3.5 x 10(-14) M. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据