期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 36, 期 4, 页码 1118-1126出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2015.2327
关键词
curcumin; apoptosis; mitochondrial signaling pathway; MAPK pathway; oxidative stress
资金
- Laboratory of Cancer Epigenetics, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University
- Zhejiang Provincial Natural Science Foundation of China [64212006]
Curcumin has been found to exhibit anticancer activity and certain studies have shown that curcumin triggers the apoptosis of human A549 lung adenocarcinoma cells. However, the mechanism underlying curcumin-mediated apoptosis is not completely understood. The present study was designed to investigate the effect of curcumin on the induction of apoptosis and apoptosis-related factors in human A549 lung adenocarcinoma cells. Treatment of A549 cells with curcumin caused a concentration-dependent inhibition of cell growth and an increase in apoptosis, as confirmed by THE MTT assay, flow cytometry and morphology analysis. Curcumin-treatment of A549 cells induced a loss of the mitochondrial membrane potential and increased cytosolic cytochrome c. Furthermore, curcumin-induced apoptosis was accompanied by changes in intracellular oxidative stress-related enzymes, including decreased intracellular reactive oxygen species levels, increased superoxide dismutase and decreased malondialdehyde and 4-hydroxynonenal. In addition, induction of apoptosis was also accompanied by phosphorylation and activation of mitogen-activated protein kinase signaling pathway factors c-Jun N-terminal kinase, p38 and extracellular signal-regulated kinase.
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