期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 35, 期 3, 页码 607-616出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2015.2073
关键词
manganese complex; anticancer; autophagy; apoptosis; reactive oxygen species
资金
- National Natural Science Foundation of China [21271090]
- Natural Science Foundation of Jiangsu Province [BK2012710]
- Jiangsu University [13JDG064]
- Graduate Research and Innovation Projects in Jiangsu Province [1293000504]
In this study, the antitumor activity of the novel manganese (II) compound, Adpa-Mn {[(Adpa)Mn(C1)(H2O)] (Adpa=bis(2-pyridylmethyl)amino-2-propionic acid)}, and its possible mechanisms of action were investigated. In vitro, the growth inhibitory effects of Adpa-Mn (with IC50 values lower than 15 mu M) on tumor cell lines were examined by MTT assay. We found that this compound was more selective against cancer cells than the popular chemotherapeutic reagent, cisplatin. We then found that Adpa-Mn achieved its selectivity against cancer cells through the transferrin (Tf)-transferrin receptor (TfR) system, which is highly expressed in tumor cells. Furthermore, Adpa-Mn induced both apoptosis and autophagy, as indicated by chromatin condensation, the activation of poly(ADP-ribose) polymerase (PARP), Annexin V/propidium iodide staining, an enhanced fluorescence intensity of monodansylcadaverine (MDC), as well as the elevated expression of the autophagy-related protein, microtubule-associated protein 1 light chain 3 (LC3). In addition, Adpa-Mn induced the generation of intracellular reactive oxygen species (ROS) and its anticancer effects were significantly reduced following pre-treatment with the antioxidant, N-acetyl cysteine, indicating that ROS triggered cell death. In vivo, the induction of apoptosis and autophagy in tumor tissue was confirmed following treatment with Adpa-Mn, which contributed to its significant antitumor activity against hepatocellular carcinoma (Hep-A cell) xenografts at 10 mg/kg. Taken together, these data suggest the possible use of Adpa-Mn as a novel anticancer drug.
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