Article
Biochemistry & Molecular Biology
Joana R. B. Carvalho, Andreia N. Meireles, Sara S. Marques, Bruno J. R. Gregorio, Ines I. Ramos, Eduarda M. P. Silva, Luisa Barreiros, Marcela A. Segundo
Summary: The analysis and interpretation of data from ORAC assays are difficult. This study compares the expression of ORAC values as AUC and lag time. It shows that some antioxidant compounds have radical scavenging reactions that extend beyond the lag time. Ethanol delays the reaction of FL and antioxidant compounds with free radicals. The choice of calibrator compounds is important to prevent biased estimation of antioxidant capacity.
Article
Chemistry, Applied
Fuyuan Zhang, Yamei Li, Xiaomeng Li, Ruobing Liu, Yaxin Sang, Xianghong Wang, Shuo Wang
Summary: A sensor based on Au@Cu-HCF nanozyme was developed for measuring the total antioxidant capacity (TAC) of plant-derived food samples. The sensor utilizes a color change to determine TAC and showed good agreement with commercial kit results.
Article
Biochemistry & Molecular Biology
Oscar Vidal-Casanella, Javier Moreno-Merchan, Merce Granados, Oscar Nunez, Javier Saurina, Sonia Sentellas
Summary: This study estimated the total polyphenol content and antioxidant capacity in various food and nutraceutical samples using antioxidant assays and chromatographic analysis. The results showed that HPLC fingerprints were suitable for classifying samples based on their nature, and the chromatographic data were correlated with antioxidant data. Regression models based on chromatographic data demonstrated good prediction capacities in estimating the antioxidant activity.
Review
Food Science & Technology
Ines Dominguez-Lopez, Maria Perez, Rosa M. Lamuela-Raventos
Summary: The Folin-Ciocalteu method is a widely used assay for measuring total (poly)phenol content in food/plant products. Recently, there has been interest in applying this method to human samples, but interference substances in biological matrices must be eliminated beforehand. This mini-review summarizes the current knowledge on using the Folin-Ciocalteu assay to measure total phenolic content in human urine and blood samples, as well as the preceding cleaning methods to remove interferences.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Muhammet Atasoy, Dilek Yildiz, Ibrahim Kula, Ali Imran Vaizogullar
Summary: A sensitive analytical technique was developed for the determination of methyl mercury and total mercury in fish tissue samples, achieving good results.
Article
Nutrition & Dietetics
Fatemeh Toorang, Monireh Sadat Seyyedsalehi, Bahareh Sasanfar, Hamideh Rashidian, Maryam Hadji, Elham Mohebbi, Roya Safari, Farid Najefi, Ahmad Naghibzadeh-Tahami, Paolo Bofetta, Kazem Zendehdel
Summary: A diet rich in antioxidants can reduce the risk of head and neck cancer and its subtypes.
FRONTIERS IN NUTRITION
(2023)
Article
Infectious Diseases
Takashi Ueda, Yoshio Takesue, Kazuhiko Nakajima, Kaoru Ichiki, Kaori Ishikawa, Kumiko Yamada, Toshie Tsuchida, Naruhito Otani, Yoshiko Takahashi, Mika Ishihara, Shingo Takubo, Hiroki Ikeuchi, Motoi Uchino, Toshimi Kimura, Kazuaki Matsumoto, Kazutaka Oda, Takeshi Kimura
Summary: AUC-guided vancomycin treatment can reduce nephrotoxicity, and obtaining two samples for AUC estimation is preferable. Significant associations were found between AUC cutoff values and early treatment response as well as nephrotoxicity rate, particularly in patients receiving vancomycin every 12 hours.
Article
Chemistry, Applied
Thangavelu Kokulnathan, Ramakrishnan Vishnuraj, Shen-Ming Chen, Biji Pullithadathil, Faheem Ahmed, P. M. Z. Hasan, Anwar L. Bilgrami, Shalendra Kumar
Summary: A one-dimensional titanium dioxide encapsulated with gold heterojunction nanofibers was successfully synthesized and applied as robust electrocatalysts for the detection of diphenylamine, showing enhanced detection activity. The modified electrode also exhibited good selectivity towards the electrochemical oxidation of DPA and proved effective in detecting the effect of DPA on spiked content in food samples.
Article
Environmental Sciences
Xiangguang Ji, Cheng Liu, Yang Wang, Qihou Hu, Hua Lin, Fei Zhao, Chengzhi Xing, Guiqian Tang, Jinqiang Zhang, Thomas Wagner
Summary: This study successfully retrieved tropospheric ozone (O3) profiles from MAX-DOAS measurements in Beijing by fusing data from multiple sources. The results showed good agreement with independent measurements at the surface and at elevated altitudes. However, limitations in the measurement technique made it difficult to reproduce the vertical distribution and steep gradients in the upper troposphere.
REMOTE SENSING OF ENVIRONMENT
(2023)
Article
Food Science & Technology
Pierluigi Piras, Nicolo Pietro Paolo Macciotta, Domenico Meloni, Andrea Sanna, Maurizio Cossu, Severyn Salis, Giannina Chessa
Summary: A total of 30 samples of Atlantic bluefin tuna were analyzed for total mercury concentration. The relationships between total mercury content and age, Fulton's condition index (K) and fat content were statistically evaluated. The effect of muscle status on mercury content was also investigated. The results showed that canning promotes a significant increase in mercury concentration. The weight of fish, K and fat content are useful tools to estimate the mercury accumulation in Atlantic bluefin tuna. This study contributes to the assessment of EU mercury levels in Atlantic bluefin tuna.
Article
Biochemistry & Molecular Biology
Lilian Alnsour, Reem Issa, Shady Awwad, Dima Albals, Idrees Al-Momani
Summary: This study aimed to determine the content of total phenols and antioxidants in coffee samples of different origins and investigate the effect of roasting on these compounds. The results showed that medium roasted coffee had the highest total phenol content, while green coffee had the highest antioxidant content. Only light and medium roasted coffee showed a significant correlation between total phenolic and antioxidant content. The antioxidant content was negatively correlated with the degree of roasting.
Article
Biochemistry & Molecular Biology
Nazli Boke Sarikahya, Ekin Varol, Gaye Sumer Okkali, Banu Yucel, Rodica Margaoan, Ayse Nalbantsoy
Summary: Propolis, a valuable natural substance, varies in properties due to botanical origin and hive location. Its color, influenced by flower markers, pollen, and nectar, is related to chemical content. This study determined color indexes of Turkish propolis samples using a Lovibond Tintometer for the first time and investigated the relationship between color index, phenolic content, and activities.
Article
Spectroscopy
Guo-Qi Zhang, Wei-Wu, Yu-Han Shi, Yang Zhao, Zhi-Hong Xu
Summary: In this study, stable and highly active cobalt and nitrogen co-doped carbon dots nanozyme was fabricated, and a high sensitivity and automated method for detecting ascorbic acid was developed. The method was also responsive to other reducing substances, allowing for the determination of total antioxidant capacity and accurate and rapid detection of actual human samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Medicine, General & Internal
Umer Ilyas, Wajida Perveen, Muhammad Akhtar, Riaz Hashmi, Ijaz Amin, Zahid Mehmood Bhatti
Summary: This study aimed to assess the effects of preoperative rehabilitation on post-operative quadriceps lag. The results showed a correlation between preoperative rehabilitation and increased post-operative extensor lag.
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2022)
Article
Plant Sciences
Mostafa El Khomsi, Mohammed Kara, Anouar Hmamou, Amine Assouguem, Omkulthom Al Kamaly, Asmaa Saleh, Sezai Ercisli, Hafize Fidan, Driss Hmouni
Summary: This research investigated the antioxidant and antimicrobial properties of hydroethanolic extracts from the leaves and roots of Cynara humilis. The findings showed that the leaf extracts had higher levels of flavonoids and polyphenols, and exhibited significant antioxidant and antimicrobial effects.
Article
Biochemistry & Molecular Biology
Gabrielle Macedo Pereira, Leticia Gondim Lambert Moreira, Themistocles da Silva Negreiros Neto, Wamberto Alristenio Moreira de Almeida, Jailma Almeida-Lima, Hugo Alexandre Oliveira Rocha, Euzebio Guimaraes Barbosa, Jose Angelo S. Zuanazzi, Mauro Vieira de Almeida, Richard Michael Grazuld, Armando Navarro-Vazquez, Fernando Hallwass, Leandro de Santis Ferreira, Matheus de Freitas Fernandes-Pedrosa, Raquel Brandt Giordani
Article
Biotechnology & Applied Microbiology
Andressa Reis, Stephanie Boutet-Mercey, Sophie Massot, Pascal Ratet, Jose Angelo Silveira Zuanazzi
BIOTECHNOLOGY LETTERS
(2019)
Article
Plant Sciences
Angelica Vestena, Luana Comerlato, Henrique Bridi, Leticia Guerini, Gari Vidal Ccana-Ccapatinta, Marcia Vignoli-Silva, Miriam Anders Apel, Saulo Fernandes, Ian Castro-Gamboa, Jose Angelo Silveira Zuanazzi, Gilsane Lino von Poser
PHYTOCHEMISTRY LETTERS
(2019)
Article
Plant Sciences
Angelica Vestena, Yasmin Piton, Sergio Augusto de Loretto Bordignon, Solange Garcia, Marcelo D. Arbo, Jose Angelo Zuanazzi, Gilsane von Poser
JOURNAL OF ETHNOPHARMACOLOGY
(2019)
Article
Multidisciplinary Sciences
Andressa Reis, Kevin Magne, Sophie Massot, Luciana R. Tallini, Marina Scope, Jaume Bastida, Pascal Ratet, Jose A. S. Zuanazzi
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
Andressa Reis, Kevin Magne, Sophie Massot, Luciana R. Tallini, Marina Scopel, Jaume Bastida, Pascal Ratet, Jose A. S. Zuanazzi
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Applied
Wamberto Alristenio Moreira de Almeida, Jean Paulo de Andrade, Daisy Sotero Chacon, Cecilia Rodrigues Lucas, Estela Mariana, Leandro de Santis Ferreira, Thais Guaratini, Euzebio Guimaraes Barbosa, Jose Angelo Zuanazzi, Fernando Hallwass, Warley de Souza Borges, Riva de Paula Oliveira, Raquel Brandt Giordani
Summary: The study evaluated seven alkaloids from Amaryllidaceae and Fabaceae for their potential against Alzheimer's disease, with Erythraline and erysodine showing greater potential compared to Memantine. These alkaloids demonstrated inhibitory effects on AD pathology in in vivo, in vitro, and in silico targets.
NATURAL PRODUCT RESEARCH
(2021)
Article
Parasitology
Brenda Petro-Silveira, Graziela Vargas Rigo, Danielle da Silva Trentin, Alexandre Jose Macedo, Elisa Sauer, Elen de Oliveira Alves, Luciana Ruschel Tallini, Solange Cristina Garcia, Warley de Souza Borges, Jose angelo Silveira Zuanazzi, Tiana Tasca
PARASITOLOGY RESEARCH
(2020)
Article
Plant Sciences
Daisy Sotero Chacon, Ivanice Bezerra da Silva, Taffarel Melo Torres, Gabrielle Macedo Pereira, Leticia Gondim Lambert Moreira, Luiz Fernando Rodrigues de Souza, Fernanda Priscila Santos Reginaldo, Alan de Araujo Roque, Jose Angelo S. Zuanazzi, Renata Libonati, Julia A. Rodrigues, Filippe L. M. Santos, Carlos Andre O. Ricart, Mariana S. Castro, Wagner Fontes, Marcelo Valle de Sousa, Arthur Germano Fett-Neto, Raquel Brandt Giordani
Summary: In this study, the label-free mass spectrometry proteome of Erythroxylum pungens (roots, barks, leaves) harvested from its natural habitat was described. It was found that processes related to defense were most important in roots, and there were different main protective proteins among organs, indicating part-specific strategies of environmental adaptation.
Article
Plant Sciences
Angelica Signor Vestena, Gabriela de Carvalho Meirelles, Jose Angelo Zuanazzi, Gilsane Lino von Poser
Summary: Asteraceae, the largest family of angiosperms, has a subfamily called Mutisioideae which is known for producing 5-methylcoumarins, compounds that are rare in plants in general. These compounds are almost exclusively found in species of this subfamily within Asteraceae. The different tribes within Mutisioideae have variations in the substitution patterns of these compounds, suggesting taxonomic significance. Most of the 5-methylcoumarins in Mutisieae species have terpene moieties attached to the coumarin scaffold, while in species of Onoserideae and Nassauvieae tribes, sesquiterpene units are predominantly attached to the alpha-pyrone moiety. Furanocoumarins, another type of coumarin, are only found in Mutisieae species, which is rare in Asteraceae. The biological properties of 5-methylcoumarins have been poorly studied.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Plant Sciences
Daisy Sotero Chacon, Marlon Dias Mariano Santos, Bernardo Bonilauri, Johnatan Vilasboa, Cibele Tesser da Costa, Ivanice Bezerra da Silva, Taffarel de Melo Torres, Thiago Ferreira de Araujo, Alan de Araujo Roque, Alan Cesar Pilon, Denise Medeiros Selegatto, Rafael Teixeira Freire, Fernanda Priscila Santos Reginaldo, Eduardo Luiz Voigt, Jose Angelo Silveira Zuanazzi, Katia Castanho Scortecci, Alberto Jose Cavalheiro, Norberto Peporine Lopes, Leandro De Santis Ferreira, Leandro Vieira dos Santos, Wagner Fontes, Marcelo Valle de Sousa, Paulo Costa Carvalho, Arthur Germano Fett-Neto, Raquel Brandt Giordani
Summary: This study focused on Erythrina velutina, a Brazilian native tree, and identified it as a rich source of bioactive phenolics, particularly flavonoids. Through metabolic and proteomic analysis, as well as integration with transcriptome data, key genes and transcription factors involved in flavonoid biosynthesis were identified. The findings provide a foundation for understanding the molecular and metabolic responses in this medicinally important species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Armando Martins dos Santos, Graziele Pereira Ramos Pedrazza, Jose Angelo Silveira Zuanazzi, Miguel Dall'Agnol, Roberto Luis Weiler, Andre Pich Brunes, Julio Antoniolli, Diogenes Cecchin Silveira
Summary: The objective of this research was to investigate the existence of root exudation of organic acid in Lotus corniculatus germplasms subjected to toxic aluminum levels and its effect on the Al content in root tissue and plant development. The experiment showed that root exudation was closely related to reduced accumulation of Al in the root tissue. The genotype UF-T2, which is tolerant to Al, excreted 80% more oxalic acid in the presence of Al compared to other germplasms.
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE
(2022)
Review
Chemistry, Medicinal
Luciana R. Tallini, Raquel B. Giordani, Jean Paulo de Andrade, Jaume Bastida, Jose Angelo S. Zuanazzi
Summary: The subfamily Amaryllidoideae, Amaryllidaceae, contains a group of structures known as Amaryllidaceae alkaloids with diverse biological activities. This review provides an update on the alkaloid profiling of Hippeastrum extracts published between 2012 and 2021, and discusses the biological potential of these compounds.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2021)
Article
Plant Sciences
Leticia Gondim Lambert Moreira, Maria Elisa Leite Ferreira, Fernanda Priscila Santos Reginaldo, Estela Mariana Guimaraes Lourenco, Jose Angelo Silveira Zuanazzi, Euzebio Guimaraes Barbosa, Leandro de Santis Ferreira, Arthur Germano Fett-Neto, Alberto Jose Cavalheiro, Ana Carolina Luchiari, Raquel Brandt Giordani
Summary: Tropane alkaloids are specialized plant metabolites found mainly in the Erythroxylaceae and Solanaceae families. Despite their structural similarities due to the tropane ring, they exhibit distinct pharmacological actions. Studies on the Erythroxylum species in Brazil, especially Erythroxylum pungens, have identified potential chemical markers like 3-(2-methylbutyryloxy)tropan-6,7-diol, which showed behavioral effects in zebrafish models. The alkaloid also demonstrated potential in affecting antidepressant pathways, highlighting its possible use in treating depression-like symptoms.
Article
Chemistry, Medicinal
Mauricio Barancelli Schwedersky, Marina Scopel, Luciana R. Tallini, Jaume Bastida, Tatiana T. Souza-Chies, Nora H. Oleas, Jose Angelo Silveira Zuanazzi
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2020)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)