Article
Cardiac & Cardiovascular Systems
Ravichandra Venkateshappa, Diana Hunter, Priya Muralidharan, Raghu S. Nagalingam, Galvin Huen, Shoaib Faizi, Shreya Luthra, Eric Lin, Yen May Cheng, Julia Hughes, Rania Khelifi, Daman Parduman Dhunna, Raj Johal, Valentine Sergeev, Sanam Shafaattalab, Lisa M. Julian, Damon T. Poburko, Zachary Laksman, Glen F. Tibbits, Tom W. Claydon
Summary: This study investigated the pathogenic mechanism of the hERG R56Q variant associated with LQTS2 and its rescue by the hERG activator RPR260243 using gene-edited hiPSC-CMs. Results showed that the hERG R56Q variant led to heterogeneous action potential duration dynamics and could be treated with RPR260243 to reduce electrical abnormalities.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qi Li, Lijuan Chai, Gaopan Dong, Xiaomeng Zhang, Lupei Du
Summary: Three environment-sensitive probes based on the nitrobenzoxadiazole fluorophore were developed for the hERG channel, with probes M1 and M3 showing high affinity for imaging in cell-based experiments. These probes offer a convenient and economical method for determining hERG channels in vitro and in cellulo, compared to other fluorescent labeling technologies, and have the potential to establish a screening system for hERG channels in physiological and pathological studies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tyler Shugg, Nimita Dave, Enoch Amarh, Abdullah A. Assiri, Karen E. Pollok, Brian R. Overholser
Summary: Aberrant expression of hERG potassium channels has been linked to the pathophysiology of glioblastoma, and letrozole has shown efficacy in pre-clinical models. This study found that letrozole inhibited hERG currents and reduced cell proliferation in glioblastoma cell lines, while hERG expression was associated with reduced overall survival in GBM patients. Further research is needed to investigate hERG expression as a potential biomarker for predicting the therapeutic potential of hERG inhibitors in GBM.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Enoch Amarh, James E. Tisdale, Brian R. Overholser
Summary: This study investigated the effects of remdesivir and its metabolite GS-441524 on hERG-related currents. It was found that acute exposure had no effect, but prolonged treatment with remdesivir significantly reduced peak tail currents and hERG current density. The propensity of remdesivir to prolong QTc intervals and induce torsade de pointes warrants further investigation.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Masahiro Kamaura, Osamu Kubo, Hiromichi Sugimoto, Naoyoshi Noguchi, Hirohisa Miyashita, Shinichi Abe, Kae Matsuda, Yoshiyuki Tsujihata, Tomoyuki Odani, Shinji Iwasaki, Toshiki Murata, Kenjiro Sato
Summary: A novel series of indolinylpyrimidine derivatives were identified as potent GPR119 agonists. Structural optimization was conducted to reduce hERG inhibitory activity while maintaining GPR119 potency, leading to the discovery of a novel piperidinone ring motif with improved drug-like properties.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Zequn Zheng, Dihui Cai, Yin Fu, Ying Wang, Yongfei Song, Jiangfang Lian
Summary: Chronic incubation with FLV and LPV led to more severe channel-blocking effects, altering channel gating and shortening action potential duration by inhibiting hERG and Cav1.2.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yusheng Qu, Robert Kirby, Richard Davies, Ayesha Jinat, Stefano Stabilini, Bin Wu, Longchuan Yu, BaoXi Gao, Hugo M. Vargas
Summary: According to International Conference on Harmonization S7B guidelines, an in vitro hERG assay is necessary for assessing the risk of delayed ventricular repolarization. Oligonucleotide therapeutics, although following the same protocol as small molecules in hERG testing, have unique mechanisms and time courses of action. A study was conducted to evaluate the time course for siRNA-mediated inhibition of hERG function and gene expression, and the results showed that a long exposure time (48h) is required for maximal reduction in hERG current.
NUCLEIC ACID THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Miaomaio Liu, Yakun Yang, Muqing Zhang, Yucong Xue, Bin Zheng, Yuanyuan Zhang, Yu Liu, Xi Chu, Zhenqing Sun, Xue Han
Summary: The study revealed that 8-Gin is the most potent inhibitor of hERG K+ channels among gingerol components, offering a new approach for understanding and treating cancer.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Bryan Queme, John C. Braisted, Patricia Dranchak, James Inglese
Summary: High throughput screening (HTS) is widely used in drug discovery and chemical biology to identify and characterize agents. Standard HTS data is usually represented as an x-y graph, while quantitative HTS (qHTS) data incorporates a third axis represented by concentration. qHTS data is challenging to display on a single graph due to the additional data points and logistic fit parameters.
JOURNAL OF CHEMINFORMATICS
(2023)
Article
Cardiac & Cardiovascular Systems
Na Wang, Yang Yang, Jing Wen, Xin-Rong Fan, Jian Li, Bing Xiong, Jin Zhang, Bo Zeng, Jian-Wu Shen, Gui-Lan Chen
Summary: Tolterodine is a first-line antimuscarinic drug for overactive bladder, causing adverse cardiac effects through inhibiting the hERG K+ channel. The drug binds to specific residues Y652 and F656 on the hERG channel, reducing currents and altering voltage dependence of channel inhibition.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Article
Chemistry, Medicinal
Shifan Ma, Zhuyezi Sun, Yankang Jing, Mark McGann, Sandor Vajda, Istvan J. Enyedy
Summary: In this study, molecular dynamics was used to optimize the structure of the droperidol-hERG complex, and solvent mapping and Atlas ligands were employed to characterize the binding sites. The use of Atlas ligands improved the identification of hERG inhibitors and enhanced the accuracy of predictions through compound alignment or docking.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Chemistry, Medicinal
Svetoslav Slavov, Jinghua Zhao, Ruili Huang, Menghang Xia, Richard Beger
Summary: A large scale experimental validation conducted in the US confirmed the predictions of our 3D-SDAR model of hERG blockage and phospholipidosis induction. It was shown that both hERG blockage and phospholipidosis induction are driven by a common three-center toxicophore composed of two aromatic rings and an amino group. Our bagging-like 3D-SDAR algorithm, which aggregates predictions from 100 randomized models, was used to predict the hERG blocking potential of all 160 drugs.
LETTERS IN DRUG DESIGN & DISCOVERY
(2023)
Article
Chemistry, Medicinal
Jing You, Wei Yang, Ronggang Ma, Anjie Xia, Guo Zhang, Zhen Fang, Nihong Guo, Shengyong Yang, Linli Li
Summary: This study reports the discovery of a series of 2-vinyl-10Hphenothiazine derivatives as new class of ferroptosis inhibitors. Compound 7j was identified as a lead compound with reduced hERG inhibition and maintained high ferroptosis inhibitory activity. Further studies showed that 7j acted as an ROS scavenger and could relieve DOX-induced cardiomyopathy.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Bernd J. Zuenkler, Maria Wos-Maganga, Stefanie Bohnet, Anne Kleinau, Detlef Manns, Shivani Chatterjee
Summary: This study investigates the binding sites of blockers for hERG and K-ATP channels from the cytoplasmic side of the plasma membrane. It reveals the presence of intracellular binding sites for these channels that cannot be dialyzed by the patch-pipette solution.
JOURNAL OF MEMBRANE BIOLOGY
(2023)
Article
Oncology
Vaishali M. Patil, Anand Gaurav, Priyanka Garg, Neeraj Masand
Summary: This study investigates the potential use of non-cancer hERG blockers as cancer therapeutics through a computational drug repurposing strategy. Twenty-six potential clinically approved compounds have been identified for further studies, with some showing potential as hERG inhibitors with anti-cancer properties. Further exploration of the proposed mechanism and experimental methodologies are needed to validate the findings and provide therapeutic solutions for late-stage cancer patients.
JOURNAL OF THE EGYPTIAN NATIONAL CANCER INSTITUTE
(2021)
Article
Biochemistry & Molecular Biology
Ashley E. Owens, Michael J. Iannotti, Tino W. Sanchez, Ty Voss, Abhijeet Kapoor, Matthew D. Hall, Juan J. Marugan, Sam Michael, Noel Southall, Mark J. Henderson
Summary: Cellular thermal shift assay (CETSA) is a valuable method for confirming target engagement in cellular environment. Traditional CETSA method measures changes in protein thermal stability using immunoblotting, which is low throughput and laborious. This study developed a high-throughput CETSA method that is compatible with 96- and 384-well microplates and removes thermally destabilized proteins using low speed centrifugation. It has been demonstrated to guide structure-activity relationship studies and compound screening.
ACS CHEMICAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Daniel Korn, Andrew J. Thieme, Vinicius M. Alves, Michael Yeakey, Joyce V. V. B. Borba, Stephen J. Capuzzi, Karamarie Fecho, Chris Bizon, Stephen W. Edwards, Rada Chirkova, Christine M. Colvis, Noel T. Southall, Christopher P. Austin, Eugene N. Muratov, Alexander Tropsha
Summary: COPs are a series of key molecular and cellular events underlying therapeutic effects of drug molecules, and their broader use with the help of biomedical knowledge graph mining is likely to accelerate drug discovery and repurposing efforts.
DRUG DISCOVERY TODAY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Dean G. Brown, Heike J. Wobst, Abhijeet Kapoor, Leslie A. Kenna, Noel Southall
NATURE REVIEWS DRUG DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Vishal B. Siramshetty, Ivan Grishagin, Dac-Trung Nguyen, Tyler Peryea, Yulia Skovpen, Oleg Stroganov, Daniel Katzel, Timothy Sheils, Ajit Jadhav, Ewy A. Mathe, Noel T. Southall
Summary: The United States has a complex regulatory scheme for marketing drugs. NCATS has developed Inxight Drugs, a web resource that integrates data from FDA, US government publications, and other sources to provide a wealth of manually curated literature data on drug ingredients and regulatory status. The database contains over 125,000 product ingredients, including approved, marketed, and investigational drugs, and is regularly updated using automated data aggregation tools.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Medicine, Research & Experimental
Karamarie Fecho, Anne E. Thessen, Sergio E. Baranzini, Chris Bizon, Jennifer J. Hadlock, Sui Huang, Ryan T. Roper, Noel Southall, Casey Ta, Paul B. Watkins, Mark D. Williams, Hao Xu, William Byrd, Vlado Dancik, Marc P. Duby, Michel Dumontier, Gustavo Glusman, Nomi L. Harris, Eugene W. Hinderer, Greg Hyde, Adam Johs, Andrew Su, Guangrong Qin, Qian Zhu
Summary: Clinical, biomedical, and translational science are facing challenges in terms of data integration and accessibility. The Biomedical Data Translator Consortium has developed a knowledge graph-based Translator system to address these challenges and provide valuable insights. The system has shown feasibility and made significant progress.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Maria Esteban-Lopez, Kenneth Wilson, Courtney Myhr, Elena Kaftanovskaya, Mark Henderson, Noel Southall, Xin Hu, Elena Barnaeva, Mare Ferrer, David Kim
Article
Chemistry, Medicinal
Jonathan H. Shrimp, John Janiszewski, Catherine Z. Chen, Miao Xu, Kelli M. Wilson, Stephen C. Kales, Philip E. Sanderson, Paul Shinn, Rick Schneider, Zina Itkin, Hui Guo, Min Shen, Carleen Klumpp-Thomas, Samuel G. Michael, Wei Zheng, Anton Simeonov, Matthew D. Hall
Summary: This study developed a suite of biochemical assays for the discovery and development of new inhibitors of TMPRSS2. Through drug repurposing, a total of 6030 compounds were screened biochemically, and the potential antiviral compounds were evaluated in cell-based experiments.
ACS INFECTIOUS DISEASES
(2022)
Article
Cell Biology
Tamara Marin, Andres E. Dulcey, Fabian Campos, Catalina de la Fuente, Mariana Acuna, Juan Castro, Claudio Pinto, Maria Jose Yanez, Cristian Cortez, David W. McGrath, Pablo J. Saez, Kirill Gorshkov, Wei Zheng, Noel Southall, Maria Carmo-Fonseca, Juan Marugan, Alejandra R. Alvarez, Silvana Zanlungo
Summary: Niemann-Pick type A (NPA) disease is a fatal lysosomal neurodegenerative disorder. Recent studies have shown that c-Abl tyrosine kinase activity plays a role in neuronal damage and death in NPA disease. There is potential for the use of c-Abl inhibitors in the clinical treatment of NPA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Kevin J. Frankowski, Samarjit Patnaik, Chen Wang, Noel Southall, Dipannita Dutta, Soumitta De, Dandan Li, Christopher Dextras, Yi-Han Lin, Marthe Bryant-Connah, Danielle Davis, Feijun Wang, Leah M. Wachsmuth, Pranav Shah, Jordan Williams, Md Kabir, Edward Zhu, Bolormaa Baljinnyam, Amy Wang, Xin Xu, John Norton, Marc Ferrer, Steve Titus, Anton Simeonov, Wei Zheng, Lesley A. Mathews Griner, Ajit Jadhav, Jeffrey Aube, Mark J. Henderson, Udo Rudloff, Frank J. Schoenen, Sui Huang, Juan J. Marugan
Summary: The perinucleolar compartment (PNC) is a subnuclear body enriched with RNA transcripts and RNA-binding proteins. PNC prevalence is positively correlated with cancer progression and metastatic capacity. A high-throughput assay was developed to identify small molecules that can reduce PNC prevalence in cancer cells. The discovery of the pyrrolopyrimidine series led to the identification of the bioavailable analogue metarrestin, which has potent antimetastatic activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Ying Fu, Juliana da Fonseca Rezende e Mello, Bryan D. D. Fleming, Alex Renn, Catherine Z. Z. Chen, Xin Hu, Miao Xu, Kirill Gorshkov, Quinlin Hanson, Wei Zheng, Emily M. M. Lee, Lalith Perera, Robert Petrovich, Manisha Pradhan, Richard T. T. Eastman, Zina Itkin, Thomas B. B. Stanley, Allen Hsu, Venkata Dandey, Kedar Sharma, William Gillette, Troy Taylor, Nitya Ramakrishnan, Shelley Perkins, Dominic Esposito, Eunkeu Oh, Kimihiro Susumu, Mason Wolak, Marc Ferrer, Matthew D. D. Hall, Mario J. J. Borgnia, Anton Simeonov
Summary: The study presents a rapid and efficient strategy for developing neutralizing humanized nanobody constructs against SARS-CoV-2. The most potent nanobody identified is capable of neutralizing the B.1.1.7 variant and withstands a specific mutation. Structural analysis and molecular dynamics simulations provide insights into the neutralization process. In an ex vivo model, the nanobody treatment effectively reduces viral burden for different SARS-CoV-2 strains.
Editorial Material
Biotechnology & Applied Microbiology
Alexandre Kiazand, Ruth Luther, Jessica Marlind Wuertele, Noel Southall, Douglas Domalik, Magnus Ysander
Summary: The COVID-19 vaccine effort was the fastest global response to a health crisis in history, allowing for the evaluation and improvement of pharmaceutical industry pharmacovigilance practices to enhance safety signal detection, evaluation, and communication.
NATURE REVIEWS DRUG DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Phillip R. Sanchez, Sarah A. Head, Shan Qian, Haibo Qiu, Avishek Roy, Zhendong Jin, Wei Zheng, Jun O. Liu
Summary: ZJ-101, a simplified analog of superstolide A, exhibits potent antiproliferative and antiadhesive effects, making it a potential therapeutic agent. Transcriptomics analysis revealed a unique dysregulation of the endomembrane system by ZJ-101, including selective inhibition of O-glycosylation. In a triple-negative breast cancer spheroid model, ZJ-101 showed potential for reversing 3D-induced chemoresistance, indicating its synergistic therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ivan Pavlinov, Mitali Tambe, Joshua Abbott, Ha Nam Nguyen, Miao Xu, Manisha Pradhan, Atena Farkhondeh, Wei Zheng
Summary: The ability to model human neurological tissues in vitro is crucial for drug development in neurological disorders. iPSC-derived brain organoids can effectively mimic protein expression patterns and physiology of specific brain regions. This study differentiated midbrain-specific brain organoids from iPSC-lines derived from healthy individuals using a matrix-free, bioreactor method. The expression of midbrain-specific neuronal markers was monitored from 7 to 90 days, and the organoids were further characterized using electron microscopy and RNA-seq. The findings provide potential benchmarks for evaluating differentiation protocols and identifying disease phenotypes in midbrain organoids derived from patient iPSC-lines with genetic neurological disorders.
Article
Public, Environmental & Occupational Health
Jiyoon Park, Malek Djelassi, Daniel Chima, Robert Hernandez, Vladimir Poroshin, Ana-Maria Iliescu, Douglas Domalik, Noel Southall
Summary: This study aims to validate an automated deep learning approach to literature surveillance for use at AstraZeneca. The results show that the model demonstrates consistent global performance compared to human surveillance teams. The prospective evaluation of model performance allows for a thorough examination of its consistency and failure modes, qualifying it for use in their surveillance processes.
Article
Microbiology
Seung Bum Park, Parker Irvin, Zongyi Hu, Mohsin Khan, Xin Hu, Qiru Zeng, Catherine Chen, Miao Xu, Madeleine Leek, Ruochen Zang, James Brett Case, Wei Zheng, Siyuan Ding, T. Jake Liang
Summary: Two hepatitis C virus fusion inhibitors have been found to broadly block human coronavirus entry into various cell types, showing effective antiviral activity against SARS-CoV-2 and its variants.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)