Article
Chemistry, Analytical
Mariam Saad, F. Rafael Castiello, Sebastien P. Faucher, Maryam Tabrizian
Summary: This study introduces a novel SPRi-based titration assay using L. pneumophila aptamers for Legionella detection. High salt buffer and 500 nM of immobilized cApt resulted in optimal signal response, with only SSC buffer facilitating incremental binding of aptamers to L-p. The assay showed the potential for SPRi-based L-p detection with specific and negligible detection of other water system microbes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Koichi Abe, Jinhee Lee, Kinuko Ueno, Ryutaro Asano, Brian Jones, Tomohiro Yamada, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Koji Sode, Toshimasa Toyo'oka, Kenichiro Todoroki, Kazunori Ikebukuro
Summary: A novel anti-idiotype aptamer A14#1 with extraordinary specificity against the anti-vascular endothelial growth factor therapeutic mAb, bevacizumab, was developed. The affinity of A14#1 to bevacizumab markedly increased at pH 4.7, enabling it to be potentially useful for therapeutic drug measurement as a novel ligand of bevacizumab.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Richard B. M. Schasfoort, Jos van Weperen, Margot van Amsterdam, Judicael Parisot, Jan Hendriks, Michelle Koerselman, Marcel Karperien, Anouk Mentink, Martin Bennink, Hans Krabbe, Leon W. M. M. Terstappen, A. H. Leontine Mulder
Summary: The SPRi assay revealed that levels and binding strength of IgG antibodies against the RBD protein increased during the course of the disease in COVID-19 patients, while levels of IgM and IgA generally decreased. Recovered patients exhibited high binding strength of IgG antibodies to the RBD protein.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Richard B. M. Schasfoort
Summary: This paper presents a new SPR-imaging method that uses a ligand density gradient to extrapolate analyte response. This method avoids cumbersome optimization procedures and minimizes surface dependent effects. The method can be fully automated for accurate determination of antibody quality from commercial sources.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Priyanka Thawany, Ashima Khanna, Umesh K. Tiwari, Akash Deep
Summary: L-cysteine conjugated molybdenum disulphide nanosheets are covalently attached to a gold coated surface plasmon resonance optical fiber to prepare a stable and reusable sensor for the detection of Ferritin and Immunoglobin G. The biosensor preparation process is characterized and verified with microscopic and spectroscopic techniques. The system shows a uniform and stable deposition of nanosheets, reproducibility, regeneration capacity, good sensitivity, and specificity for the targeted molecules.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Dapeng Sun, Yi Wu, Shwu-Jen Chang, Ching-Jung Chen, Jen-Tsai Liu
Summary: The study found that factors like pH and salt concentration of the solution can greatly affect the formation of a complex between aptamers and HbA1c. The loop L1 structure in the aptamer may be crucial for recognizing glycated hemoglobin, and the optimal detection condition was pH 6.
Article
Chemistry, Analytical
Rui Wang, Chunlan Liu, Yong Wei, Ze Ran, Tianci Jiang, Chunbiao Liu, Chen Shi, Zhuo Ren, Xingkai Wang, Zhihai Liu, Yu Zhang
Summary: In this paper, a U-shaped fiber SPR biosensor sensitized by MOFs materials was proposed and demonstrated. The sensor surface was modified with MOFs materials to enhance sensitivity, and nucleic acid aptamer was immobilized to detect MUC1 protein. The sensor showed a logarithmic linear response in the concentration range of 1 pg/ml-100 mu g/ml, with a sensitivity of 5.33 nm/log(mu g/ml) and a detection limit of 0.16 pg/ml. After MOFs sensitization, the sensor's sensitivity increased by 1.62 times and the LOD decreased by 0.75 times, making it highly sensitive and specific for clinical detection of tumor markers.
Article
Biophysics
Dapeng Sun, Jing Xie, Ching-Jung Chen, Jen-Tsai Liu
Summary: This study evaluated for the first time the interaction mechanism between glycated albumin (GA) and its aptamer by changing the pH value, salt concentration, and temperature. The results showed that the pH greatly affected the formation of the complex between the aptamer and GA, and the interaction mechanism was caused by electrostatic force. Additionally, this study also detected the protein isoelectric point (pI) using surface plasmon resonance (SPR) for the first time.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Analytical
Shen Zhang, Brett Larsen, Karen Colwill, Cassandra J. Wong, Ji-Young Youn, Anne-Claude Gingras
Summary: In this study, a simple and robust approach called turboDDA was developed to improve interactome analysis of affinity-purified samples. By eliminating the dynamic exclusion step and optimizing acquisition parameters, turboDDA detected more interactors and provided better sensitivity compared to standard DDA methods.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Reza Eivazzadeh-Keihan, Zahra Saadatidizaji, Ali Maleki, Miguel de la Guardia, Mohammad Mahdavi, Sajjad Barzegar, Samad Ahadian
Summary: Thrombin is a serine protease that plays a crucial role in blood clotting and homeostasis. Detecting thrombin quickly and accurately is important for biological analysis and clinical diagnosis. Various biosensors have been developed to achieve highly selective and specific detection of thrombin. Aptasensors, which use DNA or RNA aptamers as biological recognition elements, have shown great sensitivity and affinity in identifying thrombin.
Article
Chemistry, Multidisciplinary
Chan Ho Park, Ian A. P. Thompson, Sharon S. Newman, Linus A. Hein, Xizhen Lian, Kaiyu X. Fu, Jing Pan, Michael Eisenstein, H. Tom Soh
Summary: Cells rely on secreted signaling molecules to coordinate essential biological functions, but direct measurement of these signals is challenging. This study presents an aptamer-conjugated hydrogel matrix that enables real-time measurement of specific secreted analytes. By monitoring inter-cellular cAMP signals in amoeba cells, the researchers achieved direct measurements of oscillatory cAMP signaling. This approach can be generalized to visualize diverse extracellular signaling processes and their effects on recipient cells.
ADVANCED MATERIALS
(2023)
Review
Chemistry, Analytical
Gona K. Ali, Khalid M. Omer
Summary: This review highlights the development and advantages of diagnostic devices based on memetic molecular recognitions, particularly focusing on the hybrid recognition element of MIP-aptamer and its enhanced properties for target molecule detection in various matrices.
Article
Multidisciplinary Sciences
Alex M. Yoshikawa, Leighton Wan, Liwei Zheng, Michael Eisenstein, H. Tom Soh
Summary: This study presents a high-throughput screening process for generating high-specificity aptamers to multiple targets without the need for counterselection. The process can be easily adapted to different small-molecule analytes, accelerating the development of aptamer reagents with high specificity for target analytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Yong-Qiang He, Ying Chen, Xian-Zhu Meng, Hong -Chao Yi, Hui-Wen Gu, Xiao-Li Yin
Summary: A visual and smartphone-integrated aptasensor for rapid Staphylococcus aureus determination was developed using Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. The assay quantified the released DNAzymes via Exo III-assisted recycling reaction and analyzed them with a smartphone and UV-Vis spectrophotometer. This method exhibited good analytical performance, low cost, and portability, making it a rapid point-of-care test method with great potential in food safety control.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Bassam Bachour Junior, Marina Ribeiro Batistuti, Aline Sanches Pereira, Elisa Maria de Sousa Russo, Marcelo Mulato
Summary: An electrochemical biosensor for NS1 detection was developed in this study by optimizing the molar ratio between aptamers and MCH, achieving high sensitivity and low detection limits for NS1 detection in the early stages of disease. By adding bovine serum albumin to stabilize NS1, the sensor accurately detected NS1 from different Dengue serotypes.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)