期刊
ANALYTICAL BIOCHEMISTRY
卷 383, 期 2, 页码 159-167出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.08.034
关键词
Tulip; Nor-harmane; CHCA; DHB; Amyloglucosidase; On-probe tissue analysis
资金
- Japanese Government (Ministry of Education, Culture, Sports, Science, and Technology)
- Japan Society for the Promotion of Science (JSPS) for Scientific Research [20228004]
- UBA [X022]
- CONICET [PIP05/5443]
- ANPCyT [PICT 06-0615]
- Grants-in-Aid for Scientific Research [20228004] Funding Source: KAKEN
Underivatized carbohydrates of tulip bulb and leaf tissues were characterized in situ by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) by using carbon nano-tubes (CNTs) as matrix. Two sample preparation methods-(i) depositing CNTs on the fresh tissue slices placed on the probe and (ii) locating semitransparent tissues on a dried layer of CNTs on the probe-were examined. Furthermore, practicability of in situ starch analysis by MALDI-TOF MS was examined by detection of glucose originated from on-probe amyloglucosidase-catalyzed degradation of starch on the tissue surface. Besides, CNTs could efficiently desorb/ionize natural mono-, di-, and oligosaccharides extracted from tulip bulb tissues as well as glucose resulting from starch enzymatic degradation in vitro. These results were compared with those obtained by in situ MALDI-TOF MS analysis of similar tissues. Positive ion mode showed superior signal reproducibility. CNTs deposited under semitransparent tissue could also desorb/ionize neutral carbohydrates, leading to nearly complete elimination of matrix cluster signals but with an increase in tissue-originated signals. Furthermore, several experiments were carried out to compare the efficiency of 2,5-dihydroxybenzoic acid, nor-harmane, alpha-cyano-4-hydroxycinnamic acid, and CNTs as matrices for MALDI of neutral carbohydrates from the intact plant tissue Surface and for enzymatic tissue starch degradation; these results are discussed in brief. Among matrices studied, the lowest laser power was needed to acquire carbohydrate signals with high signal-to-noise ratio and resolution when CNTs were used. (C) 2008 Elsevier Inc. All rights reserved.
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