Article
Biochemical Research Methods
Raymond Neil Dalton, Timothy Scott Isbell, Ryan Ferguson, Louis Fiore, Andrei Malic, Jeffrey Anton DuBois
Summary: Early detection of CKD using point of care creatinine and eGFR testing improves patient management outcomes. A study was conducted to evaluate the use of a whole blood creatinine/eGFR device in a rural Nicaraguan population. Results showed a good correlation between all creatinine methods and the gold standard ID-LCMS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Food Science & Technology
Adal Mena-Garcia, Diego Herrero-Gutierrez, Maria L. Sanz, Marina Diez-Municio, Ana I. Ruiz-Matute
Summary: Food supplements made from saffron extracts are popular due to their various bioactive properties, and it is important to standardize these extracts to ensure product quality and safety. The current methods of standardization, which focus on the safranal content, may not provide accurate measurements. This study suggests using crocin isomers and trans-/cis-crocins, as well as the trans-4 GG/picrocrocin ratios, as new criteria for saffron extract standardization. The relative content of these components and their stability over 36 months in a commercial standardized saffron extract were successfully demonstrated.
Article
Food Science & Technology
Jensen Cherewyk, Taylor Grusie-Ogilvie, Barry Blakley, Ahmad Al-Dissi
Summary: This study validated a new method using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) to quantify 12 epimers of ergot alkaloids in wheat. The method showed low LOD and LOQ values, allowing for sensitive detection of ergot epimers. The study also found differences between the R and S-epimers, which has rarely been documented in previous research.
Article
Chemistry, Analytical
Bert Vankeerberghen, Jeff Op de Beeck, Gert Desmet
Summary: Due to their dimensions, micropillar array columns are ideal for high-efficiency liquid chromatography separations in proteomics. The micropillar array concept has room to progress in terms of size reduction, which can lead to even higher-efficiency separations and applications. A comparison between first-generation (Gen 1) and second-generation (Gen 2) micropillar array columns on a chip showed that reducing the pillar and interpillar size did not affect the theoretical separation potential, resulting in significant reductions in analysis time.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Kelly L. Pereira, Martyn W. Ward, John L. Wilkinson, Jonathan Brett Sallach, Daniel J. Bryant, William J. Dixon, Jacqueline F. Hamilton, Alastair C. Lewis
Summary: This study presents an automated method for non-targeted compositional analysis of environmental matrices, which has been rigorously tested for reliability and reproducibility using authentic standards. The method detected over 9600 compounds from critical pollutant sources in environmental samples, demonstrating its ability for rapid and comprehensive compound detection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemical Research Methods
David Baliu-Rodriguez, Benjamin J. Stewart, Ted J. Ognibene
Summary: Accelerator mass spectrometry (AMS) is a highly sensitive method for quantitating low amounts of 14C-labeled biomolecules, but it cannot provide structural information about the analyte. Liquid sample-AMS (LS-AMS) combines AMS with a molecular mass spectrometer to provide simultaneous quantitative AMS and structural MS data. The method was validated using 14C-labeled tryptophan and demonstrated by analyzing yeast cell extracts.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemical Research Methods
Victor Cutillas, Carmen Ferrer, Amadeo R. Fernandez-Alba
Summary: The study compared the application of liquid chromatography and supercritical fluid chromatography in pesticide residue analysis. The results showed that SFC performed better in analyzing matrices with a higher number of co-extracted compounds, attributed to the combination of chromatography separation and ion source efficiency.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Energy & Fuels
Jian-Fang Xu, Fei Wang, Xing Fan, Turghun Muhammad, Peter N. Kuznetsov, Ya-Ya Ma
Summary: This study investigated the thermal dissolution process of soft coal and hard coal using different organic solvents. By employing off-line prep-HPLC, the soluble fractions were reliably separated and enriched, leading to the identification of heavy aromatic compounds not detected by GC/MS. The differences in composition between the two coals were elucidated, providing theoretical guidance for efficient coal utilization and mine safety.
Article
Physics, Multidisciplinary
T. -Y Xia, W. -W Sun, S. Ebser, W. Jiang, G. -M Yang, H. -M Zhu, Yun-Chong Fu, F. Huang, G. -D Ming, T. Xia, Z. -T Lu
Summary: In this study, the atom-trap trace analysis method was used to measure Ca-41/Ca ratios at the level of 10(-16) and achieved a detection limit at the level of 10(-17). This method has the potential to explore the suitability of Ca-41 as a tracer.
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Petar Ristivojevic, Vesna Jovanovic, Dusanka Milojkovic Opsenica, Jihae Park, Judith M. Rollinger, Tanja Cirkovic Velickovic
Summary: A novel high-throughput methodological approach was developed for tracing compounds with radical scavenging and antimicrobial activities in brown seaweeds. Major components identified with these activities include stearidonic, eicosapentaenoic, and arachidonic acids. This method allows fast identification and quantification of bioactive compounds in multicomponent biological samples.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dong Hao Wang, Hui Gyu Park, Zhen Wang, R. J. Scott Lacombe, Vadim V. Shmanai, Andrei Bekish, Karsten Schmidt, Mikhail S. Shchepinov, J. Thomas Brenna
Summary: This study introduces a novel method for quantitatively determining the deuterium content along a hydrocarbon chain. By utilizing deuteration reactions and analysis techniques, the method enables accurate quantification of deuterium abundance at different positions of D-HUFA.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)