Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples
出版年份 2014 全文链接
标题
Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples
作者
关键词
RNA, Uncertainty, Normalisation, Reverse transcription quantitative PCR, Standardisation
出版物
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 406, Issue 26, Pages 6471-6483
出版商
Springer Nature
发表日期
2014-05-24
DOI
10.1007/s00216-014-7857-x
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Validation of high-throughput single cell analysis methodology
- (2014) Alison S. Devonshire et al. ANALYTICAL BIOCHEMISTRY
- MammaPrint Molecular Diagnostics on Formalin-Fixed, Paraffin-Embedded Tissue
- (2014) Anna Sapino et al. JOURNAL OF MOLECULAR DIAGNOSTICS
- Analytical validation of the Oncotype DX prostate cancer assay – a clinical RT-PCR assay optimized for prostate needle biopsies
- (2013) Dejan Knezevic et al. BMC GENOMICS
- Highly sensitive plasma RNA quantification by real-time PCR in HIV-2 group A and group B infection
- (2013) Séverine Delarue et al. JOURNAL OF CLINICAL VIROLOGY
- Development of a reverse transcription quantitative real-time PCR-based system for rapid detection and quantitation of hepatitis delta virus in the western Amazon region of Brazil
- (2013) Luan Felipo Botelho-Souza et al. JOURNAL OF VIROLOGICAL METHODS
- The need for transparency and good practices in the qPCR literature
- (2013) Stephen A Bustin et al. NATURE METHODS
- Evaluation of Digital PCR for Absolute RNA Quantification
- (2013) Rebecca Sanders et al. PLoS One
- Confronting diversity in the production of clinical evidence goes beyond merely including under-represented groups in clinical trials
- (2013) Karien Stronks et al. Trials
- Comparison of reverse transcription–quantitative polymerase chain reaction methods and platforms for single cell gene expression analysis
- (2012) Bridget C. Fox et al. ANALYTICAL BIOCHEMISTRY
- Simultaneously extracting DNA, RNA, and protein using kits: Is sample quantity or quality prejudiced?
- (2012) William Mathieson et al. ANALYTICAL BIOCHEMISTRY
- Quality Assessment of Biobanked Nucleic Acid Extracts for Downstream Molecular Analysis
- (2012) Karin Wahlberg et al. BIOPRESERVATION AND BIOBANKING
- Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D
- (2012) A. Katsoulidou et al. JOURNAL OF VIRAL HEPATITIS
- RT-qPCR work-flow for single-cell data analysis
- (2012) Anders Ståhlberg et al. METHODS
- Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis
- (2012) Alison S. Devonshire et al. METHODS
- Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
- (2012) Alexandra S. Whale et al. NUCLEIC ACIDS RESEARCH
- Identification of Suitable Reference Genes for Real-Time PCR Analysis of Statin-Treated Human Umbilical Vein Endothelial Cells
- (2012) Barbara Żyżyńska-Granica et al. PLoS One
- Generation of a Novel Nucleic Acid-Based Reporter System To Detect Phenotypic Susceptibility to Antibiotics in Mycobacterium tuberculosis
- (2012) M. C. Mulvey et al. mBio
- Standardisation and reporting for nucleic acid quantification
- (2011) Jim Huggett et al. ACCREDITATION AND QUALITY ASSURANCE
- Evaluation of Digital PCR for Absolute DNA Quantification
- (2011) Rebecca Sanders et al. ANALYTICAL CHEMISTRY
- RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization
- (2011) Tomas Hruz et al. BMC GENOMICS
- Applicability of RNA standards for evaluating RT-qPCR assays and platforms
- (2011) Alison S Devonshire et al. BMC GENOMICS
- Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages
- (2011) Marten B Maeß et al. BMC MOLECULAR BIOLOGY
- Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia
- (2011) Letizia Foroni et al. BRITISH JOURNAL OF HAEMATOLOGY
- Decreased adiponectin and increased inflammation expression in epicardial adipose tissue in coronary artery disease
- (2011) Yuan Zhou et al. Cardiovascular Diabetology
- Primer Sequence Disclosure: A Clarification of the MIQE Guidelines
- (2011) S. A. Bustin et al. CLINICAL CHEMISTRY
- Biomarkers for prognosis and treatment selection in advanced bladder cancer patients
- (2011) Yuanbin Ru et al. CURRENT OPINION IN UROLOGY
- The fitness for purpose of randomised experimental designs for analysis of genetically modified ingredients
- (2011) Malcolm Burns et al. EUROPEAN FOOD RESEARCH AND TECHNOLOGY
- Molecular Bacterial Load Assay, a Culture-Free Biomarker for Rapid and Accurate Quantification of Sputum Mycobacterium tuberculosis Bacillary Load during Treatment
- (2011) Isobella Honeyborne et al. JOURNAL OF CLINICAL MICROBIOLOGY
- Inclusion, Analysis, and Reporting of Sex and Race/Ethnicity in Clinical Trials: Have We Made Progress?
- (2011) Stacie E. Geller et al. JOURNAL OF WOMENS HEALTH
- Analysis of Chlamydia pneumoniae infection in mononuclear cells by reverse transcription-PCR targeted to chlamydial gene transcripts
- (2011) Laura Mannonen et al. MEDICAL MICROBIOLOGY AND IMMUNOLOGY
- Measurable impact of RNA quality on gene expression results from quantitative PCR
- (2011) Joëlle Vermeulen et al. NUCLEIC ACIDS RESEARCH
- Upregulated CD133 expression in tumorigenesis of colon cancer cells
- (2011) Zhi-Li Yang WORLD JOURNAL OF GASTROENTEROLOGY
- Comparison of Methods for Accurate Quantification of DNA Mass Concentration with Traceability to the International System of Units
- (2010) Somanath Bhat et al. ANALYTICAL CHEMISTRY
- Changed Relative to What? Housekeeping Genes and Normalization Strategies in Human Brain Gene Expression Studies
- (2010) Elizabeth M. Tunbridge et al. BIOLOGICAL PSYCHIATRY
- Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis
- (2010) Guma MK Abdeldaim et al. BMC MICROBIOLOGY
- MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
- (2010) Stephen A Bustin et al. BMC MOLECULAR BIOLOGY
- Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load
- (2010) Kiana Shahzamani et al. CLINICAL CHEMISTRY AND LABORATORY MEDICINE
- Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood T cells
- (2010) A. Q. Gomes et al. HAEMATOLOGICA
- Accuracy to 2nd International HIV-1 RNA WHO Standard: Assessment of three generations of quantitative HIV-1 RNA nucleic acid amplification tests
- (2010) Joachim Glaubitz et al. JOURNAL OF CLINICAL VIROLOGY
- Prognostic impact of clinical course-specific mRNA expression profiles in the serum of perioperative patients with esophageal cancer in the ICU: a case control study
- (2010) Shunsaku Takahashi et al. Journal of Translational Medicine
- A Pipeline to Determine RT-QPCR Control Genes for Evolutionary Studies: Application to Primate Gene Expression across Multiple Tissues
- (2010) Olivier Fedrigo et al. PLoS One
- Validation of reference gene stability for APAP hepatotoxicity studies in different in vitro systems and identification of novel potential toxicity biomarkers
- (2010) Bridget C. Fox et al. TOXICOLOGY IN VITRO
- Tumor Growth Rate Determines the Timing of Optimal Chronomodulated Treatment Schedules
- (2010) Samuel Bernard et al. PLoS Computational Biology
- Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
- (2010) Manuela Marullo et al. GENOME BIOLOGY
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
- (2009) S. A. Bustin et al. CLINICAL CHEMISTRY
- Under-representation of women and ethnic minorities in vascular surgery randomized controlled trials
- (2009) Andrew W. Hoel et al. JOURNAL OF VASCULAR SURGERY
- Statistical assessment of DNA extraction reagent lot variability in real-time quantitative PCR
- (2009) R.N. Bushon et al. LETTERS IN APPLIED MICROBIOLOGY
- Why the need for qPCR publication guidelines?—The case for MIQE
- (2009) Stephen A. Bustin METHODS
- External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
- (2009) Joëlle Vermeulen et al. NUCLEIC ACIDS RESEARCH
- Gene expression variation between distinct areas of breast cancer measured from paraffin-embedded tissue cores
- (2008) Martina Schobesberger et al. BMC CANCER
- Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)
- (2008) Hiroaki Nitta et al. Diagnostic Pathology
- Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool
- (2008) Elke Schultz-Thater et al. Journal of Translational Medicine
- A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR
- (2008) Federica Rizzi et al. PLoS One
Discover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversationAdd your recorded webinar
Do you already have a recorded webinar? Grow your audience and get more views by easily listing your recording on Peeref.
Upload Now