Article
Chemistry, Analytical
Xiaofei Wang, Lu Zhang, Yuhong Xiang, Nengsheng Ye, Kehui Liu
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has become a powerful method for studying the spatial distribution of molecules. The thickness of tissue sections is crucial for obtaining high-quality imaging data. This study investigated the effect of tissue slice thickness on MALDI MSI detection.
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemical Research Methods
Jessica L. Moore, Georgia Charkoftaki
Summary: This review discusses recent advances in imaging mass spectrometry, specifically matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. It explores sample preparation strategies, considerations for different analyte classes, and the use of multimodal imaging strategies. The review also examines experimental design approaches, standardization of processes, and data analysis platforms. It concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Nhu Q. Vu, Amanda R. Buchberger, Jillian Johnson, Lingjun Li
Summary: Neuropeptides are low abundance signaling molecules that modulate physiological processes, and mass spectrometry imaging is increasingly useful in studying them. Proper tissue washes can increase detectable neuropeptides, and using a salt solution wash may affect neuropeptide detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Spectroscopy
Colin T. McDowell, Xiaowei Lu, Anand S. Mehta, Peggi M. Angel, Richard R. Drake
Summary: This article introduces the development of glycan imaging mass spectrometry and discusses its applications in different sample types. Glycan imaging mass spectrometry analyses broaden our understanding of the biological and clinical relevance of N-glycosylation to human disease.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Physical
Paulina Kret, Anna Bodzon-Kulakowska, Anna Drabik, Joanna Ner-Kluza, Piotr Suder, Marek Smoluch
Summary: The science related to biomaterials and tissue engineering is expanding, with a focus on surface modifications and interactions with surrounding tissues. Mass Spectrometry Imaging (MSI) techniques can measure molecular composition from biomaterials and adjacent tissue simultaneously, without the need for antibodies or dyes that may affect the results.
Article
Multidisciplinary Sciences
John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Summary: This study introduces a semi-automated single-ion mass spectrometry workflow for top-down tandem mass spectrometry analysis directly from tissue microenvironments. The results demonstrate that this method can identify multiple proteoforms in tumor samples within a short period of time, and effectively identify proteoform signatures in tumor and stromal regions.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Lianlian Ma, Qingrong Xie, Mingyi Du, Yudi Huang, Yingying Chen, Dong Chen, Yizhu Xu, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: This study systematically investigated the optimized sample preparation conditions for insects and model organisms in mass spectrometry imaging (MSI), providing guidelines for MSI analysis and expanding its potential in life sciences and environmental sciences.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Ethan Yang, Jeong Hee Kim, Caitlin M. Tressler, Xinyi Elaine Shen, Dalton R. Brown, Cole C. Johnson, Tae -Hun Hahm, Ishan Barman, Kristine Glunde
Summary: Multimodal tissue imaging techniques, such as RSI and MALDI MSI, have the potential to provide spatially resolved, sensitive, specific biomolecular information for unraveling biological processes and identifying disease biomarkers. The development of RaMALDI, a streamlined, integrated workflow, allows for the combined imaging of RSI and MALDI MSI on a single tissue section with one sample preparation protocol, effectively integrating molecular information and driving discoveries in various fields.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Tanja Bien, Sebastian Bessler, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the potential to visualize the spatial distribution of biomolecules in cell cultures and tissue sections with high resolution, but sample preparation is crucial. A study found that a robust sample preparation protocol can preserve cell morphology and molecular integrity, contributing to the study of cell-to-cell heterogeneities at a molecular level. Advancements in single-cell MALDI imaging could benefit from these protocols.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Zeljko Debeljak, Ann-Christin Niehoff, Ana Bandjak, Dario Mandic, Bojana Bosnjak, Marija Heffer, Stefan Mrdenovic, Ivana Markovic, Milorad Zjalic, Vatroslav Seric
Summary: This study aimed to develop and evaluate matrix-assisted laser desorption ionization time-of-flight mass spectrometry imaging of blood smears. Different blood plasma removal, matrix deposition, and instrumental settings were evaluated, and the study sets the foundations for future application of this technique in clinical diagnostics and research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Rita Casadonte, Mark Kriegsmann, Katharina Kriegsmann, Isabella Hauk, Rolf R. Meliss, Cornelia S. L. Mueller, Joerg Kriegsmann
Summary: This study utilized imaging mass spectrometry to compare the proteomic profiles of cutaneous melanomas and melanocytic nevi in a large cohort, identifying a signature that can distinguish malignant melanoma from benign nevus with an overall accuracy of over 98%. The prediction model was validated in an independent set with an accuracy of 93% in classifying melanoma from nevi. Statistical analysis of the imaging mass spectrometry data revealed significant variations in mass-to-charge ratio peaks between the two tissue types. This study represents the largest imaging mass spectrometry investigation of cutaneous melanoma and nevi to date, demonstrating the potential for IMS to discriminate between melanocytic nevi and melanoma.
Article
Biochemistry & Molecular Biology
Andreas Dannhorn, Maria Luisa Doria, James McKenzie, Paolo Inglese, John G. G. Swales, Gregory Hamm, Nicole Strittmatter, Gareth Maglennon, Sadaf Ghaem-Maghami, Richard J. A. Goodwin, Zoltan Takats
Summary: In this study, a method called analyte-targeted DESI-MSI utilizing a TQ mass analyzer was used for faster MSI data acquisition. The platform operated successfully at a scanning rate of 10 scans/s, offering improved sensitivity compared to conventional TOF mass analyzers. It has the potential for pharmaceutical research and development, as it enhances compound coverage and reduces dataset size.