Article
Biochemical Research Methods
Ashley Di Meo, Dorsa Sohaei, Ihor Batruch, Pantelis Alexandrou, Ioannis Prassas, Eleftherios P. Diamandis
Summary: The analysis utilized LC-MS/MS technology to profile the normal human proteome from 117 samples across 46 normal tissues and organs, quantifying 10,438 unique proteins. By combining data from biological fluid proteomics of healthy individuals, tandem mass spectra corresponding to 13,028 unique human protein-coding genes were generated, serving as an important complementary resource for future biomarker discovery.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Plant Sciences
Ivana Carev, Cengiz Sarikurkcu
Summary: The study demonstrated the significant antioxidant activity of the endemic Stachys species S. cretica ssp. anatolica in Turkey. The extracts of this plant are rich in polyphenols, showing strong activity against antioxidant enzymes and potential therapeutic effects for skin disorders and diabetes.
Article
Chemistry, Applied
Hanbin Xu, Jiaping Zhou, Jinglin Yu, Shuo Wang, Shujun Wang
Summary: The study found that gluten and its hydrolysates decreased wheat starch digestibility by inhibiting the activity of α-amylase and interacting with starch through hydrogen bonding. Additionally, hydrolyzed gluten GH-1 displayed a greater inhibitory effect and stronger interaction with starch than other gluten/hydrolysates, indicating a significant impact on starch digestibility.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemical Research Methods
Michal Lesnak, Dominik Jursa, Marek Miskay, Helena Riedlova, Karla Barcova, Milan Adamek
Summary: Surface plasmon resonance imaging biosensors offer advantages such as label-free detection, high sensitivity to low protein concentrations, and the ability for repeated measurements over time. This study successfully created and analyzed biochips for testing cystatin C in patient urine samples, demonstrating the method's sensitivity even for very low concentrations. The main advantage of surface plasmon resonance imaging is its label-free and real-time analysis capabilities, with high sensitivity and specificity.
Article
Agriculture, Dairy & Animal Science
Jakub Biesek, Sebastian Wlazlak, Marek Adamski
Summary: The study assessed the quality characteristics of hatching eggs during the early-mid incubation period. It found that the yolk vitelline membrane strength and the albumen viscosity and lysozyme activity changed significantly with incubation days. These findings indicate that the protective barriers of the embryo are altered during incubation, and lysozyme is transferred from the albumen to the amniotic fluid due to its activity.
Article
Chemistry, Analytical
Nour Sharar, Konstantin Wuestefeld, Rahat Morad Talukder, Julija Skolnik, Katharina Kaufmann, Bernd Giebel, Verena Boerger, Friedrich Nolte, Carsten Watzl, Frank Weichert, Roland Hergenroeder, Victoria Shpacovitch
Summary: This study introduces a wide-field surface plasmon resonance microscopy sensor that utilizes the surface plasmon resonance phenomenon to detect biological and non-biological nanoparticles. The sensor is able to detect and quantify biological nanoparticles without the need for labeling, using target-specific antibodies. The research also includes modifications to the optical sensor system and evaluates their effectiveness.
Article
Chemistry, Analytical
Luyao Liu, Chaowei Han, Meng Jiang, Tiantian Zhang, Qing Kang, Xiaoying Wang, Pengcheng Wang, Feimeng Zhou
Summary: The tris-NTA chip shows strong retention and uniform orientation of His-tagged proteins, enabling accurate quantification of IgG molecules and establishing optimal calibration curve construction time. The amount of immobilized His-protein is highly dependent on protein pI. The study outlines experimental parameters and procedures to expand SPR applications involving His-tagged proteins.
ANALYTICA CHIMICA ACTA
(2021)
Article
Ophthalmology
Maite Lopez-Lopez, Uxia Regueiro, Susana B. Bravo, Maria del Pilar Chantada-Vazquez, Ruben Varela-Fernandez, Paulo Avila-Gomez, Pablo Hervella, Isabel Lema
Summary: Utilizing SWATH-MS for tear protein quantification, dysregulated proteins involved in iron homeostasis, inflammatory-immune response, oxidative stress, and other biological processes were identified in patients with keratoconus. The study sheds light on the molecular mechanisms underlying the disease and highlights the potential therapeutic targets related to iron accumulation and tissue damage in keratoconus pathophysiology.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Federica Carnamucio, Donatella Aiello, Claudia Foti, Anna Napoli, Ottavia Giuffre
Summary: Nalidixic acid (NAL) is a broad-spectrum antimicrobial commonly used for urinary tract infections. Complexation of NAL with metal cations like Zn2+, Mn2+, and Cu2+ can enhance its efficiency and potency. This study investigates the behavior of NAL in solution and its interaction with metal cations under different conditions to better understand the influence of complexation on NAL and propose novel formulations. Spectrophotometric titrations were conducted to determine the protonation constants and acid-base behavior of NAL. Potentiometric and spectrophotometric titrations were then carried out to study the interaction between NAL and Zn2+, Mn2+, and Cu2+, allowing the determination of formation constants and sequestering ability. Laser desorption ionization mass spectrometry and MS/MS analyses confirmed the formation of complex species between NAL and the metal cations.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maryam Azimirad, Mohsen Zaheri, Fatemeh Javaheri-Ghezeldizaj, Reza Yekta, Jafar Ezzati Nazhad Dolatabadi
Summary: This study evaluated the interaction between sodium acid pyrophosphate (SAPP) and bovine serum albumin (BSA), revealing the formation of a complex through a static quenching mechanism. Hydrogen bonds and van der Waals forces were identified as the primary forces involved in the binding processes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Physical
Hasan Khaled Rouf, Tauhidul Haque
Summary: This paper introduces a graphene-MoSe2 enhanced SPR biosensor with improved sensitivity compared to traditional SPR sensors and graphene-based sensors, albeit with slightly compromised quality factor. The sensor's detection capability for biological analytes has been demonstrated.
Article
Chemistry, Applied
Peilin Guo, Xiaokang An, Wuying Chen, Xinglu Pan, Runan Li, Jun Xu, Xiaohu Wu, Yongquan Zheng, Fengshou Dong
Summary: In this study, fluindapyr enantiomers were successfully separated by supercritical fluid chromatography-mass spectrometry, and it was found that the enantiomers exhibited different degradation behavior in different plants.
Article
Chemistry, Multidisciplinary
Peiling Wu, Le Sun, Shunyao Qi, Tiexin Zeng, Ziyu Hou, Yun Yang, Lijia Xu, Jianhe Wei, Peigen Xiao
Summary: In this study, agarwood leaf aromatic oil was extracted using supercritical carbon dioxide (SC-CO2) extraction and compared with traditional hydrodistillation (HD) and Soxhlet extraction (SE) methods. The results showed that SC-CO2 extraction had higher yields, better smell, and appearance compared to the other two methods. The analysis identified the constituents of the aromatic oil and revealed its antioxidant, anti-inflammatory, and anti-melanogenesis activities. This work demonstrates the great potential of SC-CO2-extracted agarwood leaf aromatic oil as a food additive or dietary supplement.
JOURNAL OF CO2 UTILIZATION
(2023)
Article
Engineering, Electrical & Electronic
Nunzio Cennamo, Francesco Arcadio, Vincenzo Marletta, Salvatore Baglio, Luigi Zeni, Bruno Ando
Summary: This study introduces a novel methodology for magnetic field sensing using SPR sensors and magnetic fluids. By utilizing fiber optics and ferrofluid technology, reliable magnetic field measurements with high sensitivity and resolution have been achieved. The comparison between simulated and measured values also shows a good match, demonstrating the effectiveness of the proposed sensing approach.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Chemistry, Analytical
Zhangjie Wang, Vijay M. Dhurandhare, Cressida A. Mahung, Katelyn Arnold, Jine Li, Guowei Su, Ding Xu, Rob Maile, Jian Liu
Summary: Heparan sulfates (HSs) are important glycans in animals, and a new analysis technique can accurately determine the amount and disaccharide composition of HS, aiding in understanding its role in diseases. The method can be used to assess levels of HS in plasma/serum and tissues/organs, potentially providing new biomarkers for disease diagnosis and prognosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Instruments & Instrumentation
A. Baud, L. Ayme, F. Gonnet, I. Salard, Y. Gohon, P. Jolivet, K. Brodolin, P. Da Silva, A. Giuliani, B. Sclavi, T. Chardot, P. Mercere, P. Roblin, R. Daniel
JOURNAL OF SYNCHROTRON RADIATION
(2017)
Article
Biochemistry & Molecular Biology
Isabelle Compagnon, Baptiste Schindler, Gina Renois-Predelus, Regis Daniel
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2018)
Article
Immunology
Nicolas S. Merle, Anne Grunenwald, Marie-Lucile Figueres, Sophie Chauvet, Marie Daugan, Samantha Knockaert, Tania Robe-Rybkine, Remi Noe, Olivia May, Marie Frimat, Nathan Brinkman, Thomas Gentinetta, Sylvia Miescher, Pascal Houillier, Veronique Legros, Florence Gonnet, Olivier P. Blanc-Brude, Marion Rabant, Regis Daniel, Jordan D. Dimitrov, Lubka T. Roumenina
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Chemistry, Physical
Aziz Fennouri, Joana Ramiandrisoa, Laurent Bacri, Jerome Mathe, Regis Daniel
EUROPEAN PHYSICAL JOURNAL E
(2018)
Article
Biochemical Research Methods
Cedric Przybylski, Florence Gonnet, Els Saesen, Hugues Lortat-Jacob, Regis Daniel
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Wenshuang Wang, Cedric Przybylski, Xiaojuan Cai, Chrystel Lopin-Bon, Runmiao Jiao, Liran Shi, Kazuyuki Sugahara, Jose L. Neira, Regis Daniel, Fuchuan Li
Summary: This study identified a novel CS/DS 4-O-endosulfatase from a marine bacterium and investigated its catalytic mechanism, revealing substrate specificities, action patterns, and a decrease in enzyme activity with increasing substrate size. The deeper understanding of this enzyme provides a useful tool for exploring the structure-function relationship of CS/DS.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Salome Poyer, Ilham Seffouh, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jose L. Neira, Jean-Yves Salpin, Regis Daniel
Summary: The study developed a new analytical method for efficient differentiation and sensitive detection of different structures of chondroitin sulfate glycosaminoglycans, which was applied to analyze the disaccharide composition of biological reaction media.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Rana El Masri, Amal Seffouh, Caroline Roelants, Ilham Seffouh, Evelyne Gout, Julien Perard, Fabien Dalonneau, Kazuchika Nishitsuji, Fredrik Noborn, Mahnaz Nikpour, Goran Larson, Yoann Cretinon, Melanie Friedel-Arboleas, Kenji Uchimura, Regis Daniel, Hugues Lortat-Jacob, Odile Filhol, Romain R. Vives
Summary: This study found that human HSulf-2 enzyme carries a chondroitin/dermatan sulfate glycosaminoglycan (GAG) chain, which regulates the enzyme's activity and is promoted by mammalian hyaluronidase.
Article
Chemistry, Physical
Mazdak Afshar Bakshloo, Safia Yahiaoui, Fabien Piguet, Manuela Pastoriza-Gallego, Regis Daniel, Jerome Mathe, John J. Kasianowicz, Abdelghani Oukhaled
Summary: This study investigates a method for protein identification using nanopores. The capture rate and dwell time of polypeptide fragments in the pore are found to depend on the ionic strength and magnitude of the applied voltage. The method is capable of accurately identifying proteins, but further research is needed to optimize the analysis.
Article
Multidisciplinary Sciences
Parisa Bayat, Charlotte Rambaud, Bernard Priem, Matthieu Bourderioux, Melanie Bilong, Salome Poyer, Manuela Pastoriza-Gallego, Abdelghani Oukhaled, Jerome Mathe, Regis Daniel
Summary: This study demonstrates the label-free detection and analysis of glycosaminoglycan molecules using biological nanopores, reaching a high resolution in deciphering structural information. It is an important step towards the ultimate goal of glycosaminoglycan sequencing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Mazdak Afshar Bakshloo, Safia Yahiaoui, Matthieu Bourderioux, Regis Daniel, Manuela Pastoriza-Gallego, John J. Kasianowicz, Abdelghani Oukhaled
Summary: Alpha-synuclein plays a crucial role in regulating nerve activity. Mutations in this protein can lead to its aggregation and fibril formation, which is associated with neurodegenerative diseases like Parkinson's. Our study demonstrates that a nanometer-scale pore can effectively distinguish between different forms of alpha-synuclein, including wild-type, mutated, and post-translationally modified variants.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Melanie Bilong, Parisa Bayat, Matthieu Bourderioux, Murielle Jerome, Alexandre Giuliani, Regis Daniel
Summary: Mammalian hyaluronidases play a role in the catabolism of chondroitin sulfate, producing a wide range of oligosaccharides from CS-A and CS-C. Structural investigation using mass spectrometry reveals selective glycosidic bond cleavage catalyzed by mammalian hyaluronidase, supporting its role in CS metabolism. Additionally, differences in oligosaccharide distribution between CS-A and CS-C digestion mixtures have been observed.
Article
Biochemistry & Molecular Biology
Ilham Seffouh, Cedric Przybylski, Amal Seffouh, Rana El Masri, Romain R. Vives, Florence Gonnet, Regis Daniel
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2019)