Article
Chemistry, Analytical
Xuetong Qu, Tao Wang, Xingyue Liu, Xinrong Jiang, Xiao Liang, Jianmin Wu
Summary: A dual-mechanism-driven strategy for high-coverage detection of serum lipids was proposed, using a novel SALDI-MS target composed of composite nanostructures. The strategy enabled simultaneous detection of multiple lipid types in a fast analysis. Furthermore, the method demonstrated high sensitivity and specificity in diagnosing hepatocellular carcinoma.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jian Chen, Rendi Yang, Yongzheng Wang, Shigenobu Koseki, Linglin Fu, Yanbo
Summary: In this study, the inhibitory effect of D-Tryptophan (D-Trp) on bacterial growth and spoilage potential was investigated. It was found that higher levels of salt and D-Trp were required for optimal growth inhibition. The combination of D-Trp and salt also showed a reduced growth of psychrotrophic bacteria at low temperature.
Article
Chemistry, Applied
Jie Wang, Jie Liao, Honghai Wang, Xiaofang Zhu, Linqiu Li, Weibo Lu, Gongshuai Song, Qing Shen
Summary: A method for analyzing plasmalogens in shellfish was developed, with mussel identified as having the highest content and being a good choice for large-scale preparation.
Article
Chemistry, Applied
Jie Chen, Qing Kong, Ziting Sun, Jiayuan Liu
Summary: The study using liquid chromatography-mass spectrometry found that lipid composition in salmon muscle changes over time when stored at 4 degrees Celsius, with four lipids showing significant increase from the tenth day. Traditional freshness index also indicated spoilage of salmon slices by the tenth day, suggesting they may serve as indicators of raw salmon freshness.
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Polymer Science
Lidia Molina-Millan, Aljoscha Koerber, Bryn Flinders, Berta Cillero-Pastor, Eva Cuypers, Ron M. A. Heeren
Summary: Synthetic polymers, when improperly disposed of, accumulate in the environment and pose potential health risks by degrading into micro- and nanoparticles (MNPs). Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) can be used to study biological or toxicological changes in organisms exposed to MNPs. MALDI-2 has been found to enhance the sensitivity of polymer analysis, making it a promising tool for studying the distribution of polymers in biological systems.
Article
Biochemical Research Methods
Matthew J. Sorensen, Kelsey E. Miller, James W. Jorgenson, Robert T. Kennedy
Summary: In this study, a two-dimensional liquid chromatography-mass spectrometry method was developed for lipidomics analysis of human plasma. The method achieved good orthogonality and high peak capacity, and detected over 1000 lipids from a small amount of plasma sample.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Hana Studentova, Bohuslav Melichar, Michal Holcapek
Summary: This study systematically investigates the effects of different lipid class separation methods and mass spectrometers on quantitative lipidomic data, finding slight variations in molar concentrations even within the same laboratory using different platforms. Normalization to a reference sample can significantly reduce these differences, leading to highly homogenous molar concentrations of individual lipid species. This strategy suggests a potential approach for reporting comparable quantitative results regardless of the analytical platform and mass spectrometer used.
Article
Biochemical Research Methods
Matthias Hermann, Prashant Agrawal, Chang Liu, J. C. Yves LeBlanc, Thomas R. Covey, Richard D. Oleschuk
Summary: The rapid calibration chip (RCC) uses the wetting behavior of hydrophilic/hydrophobic patterns to confine droplets and obtain a calibration curve. It doesn't require advanced equipment and can form multiple droplet series quickly. Evaluation of its performance allows for determining liquid volumes.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Polymer Science
Ulric Conway, Alexander D. Warren, Christopher J. Arthur, Paul J. Gates
Summary: The main goals in the analysis of synthetic polymers include determining molecular weight, molecular weight distribution, and chemical structure. Matrix-assisted laser desorption/ionization mass spectrometry is a commonly used technique, and using carbon-based matrices with polyethylene glycol polymers doped with LiCl is an effective method for analyzing low molecular weight polymers.
Article
Multidisciplinary Sciences
Guanhua Zhang, Chunxia Ma, Qing He, Hongjing Dong, Li Cui, Lili Li, Lingyu Li, Yan Wang, Xiao Wang
Summary: In this study, Pt@MXene was synthesized as an ideal substrate for SALDI MS, and it showed excellent performance for the high-throughput detection of small-molecule natural products. It provided stronger signal intensity, wider molecular coverage, lower background interference, good salt and protein tolerance, good repeatability, and high detection sensitivity. The Pt@MXene substrate was also successfully used for quantifying target molecules in medicinal plants. This proposed method has great potential for wide applications.
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Infectious Diseases
Marius Ahm Stauning, Christian Salgard Jensen, Trine Staalsoe, Jorgen A. L. Kurtzhals
Summary: This proof-of-concept study explores the potential of MALDI-TOF as a diagnostic tool for detecting and quantifying Plasmodium falciparum in human blood. The results show that MALDI-TOF can detect and quantify P. falciparum at clinically relevant infection intensities, providing proof-of-concept for MALDI-TOF-based diagnosis of human malaria.
Article
Chemistry, Analytical
Felina Hildebrand, Harald Schoeny, Evelyn Rampler, Gunda Koellensperger
Summary: Lipidomics studies focus on identifying and quantifying lipids comprehensively. Reversed phase liquid chromatography (RP-LC) coupled with high resolution mass spectrometry (MS) is commonly used for lipid identification, but accurate lipid quantification remains challenging. We established a dual flow injection and chromatography setup to address the issue of solvent composition affecting ionization response. Our results confirmed that changing solvent conditions significantly influence ionization response and quantification biases. This finding was further supported by quantifying human plasma lipids and a panel of lipid species standards under different ionization conditions.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Huatang Zhang, Ruochuan Liu, Yi Tan, William Haowei Xie, Haipeng Lei, Hon-Yeung Cheung, Hongyan Sun
ACS APPLIED MATERIALS & INTERFACES
(2015)
Article
Biochemical Research Methods
Yuan-Yuan Li, Rui Di, Wing-Leung Hsu, Ye-Qing Huang, Hongyan Sun, Hon-Yeung Cheung
Article
Biochemical Research Methods
Lin-Qiu Li, Yue Cai, Mei Yang, Qing Shen, Ka-Ming Yu, Hon-Yeung Cheung
Article
Chemistry, Applied
Mei Yang, Qing Shen, Lin-Qiu Li, Ye-Qing Huang, Hon-Yeung Cheung
Article
Chemistry, Applied
Qing Shen, Yanbin Lu, Zhiyuan Dai, Hon-Yeung Cheung
Article
Agriculture, Multidisciplinary
Yuan-Yuan Li, Haixing Wang, Chunyan Zhao, Ye-Qing Huang, Xiaowei Tang, Hon-Yeung Cheung
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2015)
Article
Food Science & Technology
Yuan-Yuan Li, Shengquan Hu, Ye-Qing Huang, Yifan Han, Hon-Yeung Cheung
JOURNAL OF FUNCTIONAL FOODS
(2015)
Article
Chemistry, Analytical
Zhi-feng Zhang, Lu-yang Lu, Yuan Liu, Rui Zeng, Jing Xie, Ye-qing Huang, Qing Shen, Hon-yeung Cheung
ANALYTICAL LETTERS
(2016)
Article
Chemistry, Applied
Qing Shen, Zhiyuan Dai, Yao-Wen Huang, Hon-Yeung Cheung
Article
Chemistry, Analytical
Qing Shen, Mei Yang, Linqiu Li, Hon-Yeung Cheung
ANALYTICA CHIMICA ACTA
(2014)
Article
Chemistry, Applied
Mei Yang, Sau-Ha Cheung, Sze Chun Li, Hon-Yeung Cheung
Article
Food Science & Technology
Yuan-Yuan Li, Rui Di, Joewel T. Baibado, Yu-Shan Cheng, Ye-Qing Huang, Hongyan Sun, Hon-Yeung Cheung
FOOD RESEARCH INTERNATIONAL
(2014)
Article
Agriculture, Multidisciplinary
Qing Shen, Hon-Yeung Cheung
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2014)
Article
Biochemical Research Methods
Zhiqiang Zhang, Liancai Xu, Hon-Yeung Cheung
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2014)