Article
Pharmacology & Pharmacy
Donghui Lao, Rong Liu, Jianying Liang
Summary: By applying metabolomics, potential biomarkers for AIDS patients have been identified and verified, suggesting their potential role in the diagnosis of HIV/AIDS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Haoran Guo, Qi Wang, Tao Li, Jingwen Chen, Chao Zhang, Ying Xu, Qing Chang, Hangyi Li, Weiqiang Sun, Ruidi Han, Chi Wang, Chengbin Wang
Summary: This study discovered that ascending to high altitude can lead to physiological and molecular changes, resulting in some individuals being unable to acclimatize. Predicting acute mountain sickness (AMS) prior to ascent remains a challenge. Proteomic analysis revealed significant differences in inflammatory and immune-related processes between AMS-susceptible and AMS-resistant groups. Potential biomarkers, such as SAP, AAT, LTF, and HSP90-alpha, were identified for the prediction of AMS at low altitudes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shin Koike, Yusuke Miyaji, Kazuhiro Suzuki, Mitsuhiro Miyashita, Masanari Itokawa, Makoto Arai, Yuki Ogasawara
Summary: This study measured bile acids in plasma from patients with schizophrenia and healthy subjects and found significant differences in unconjugated bile acid levels between the two groups. The results suggest that plasma bile acid levels may serve as diagnostic markers for schizophrenia.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Obstetrics & Gynecology
See Ling Loy, Jieliang Zhou, Liang Cui, Tse Yeun Tan, Tat Xin Ee, Bernard Su Min Chern, Jerry Kok Yen Chan, Yie Hou Lee
Summary: The study identified potential biomarkers for peritoneal endometriosis in peritoneal fluid and serum using metabolomics analysis. Phenylalanyl-isoleucine showed promising discriminatory performance in distinguishing peritoneal endometriosis from controls, particularly in the proliferative phase. Further research is needed to validate these findings and assess the clinical utility of phenylalanyl-isoleucine as a diagnostic biomarker for peritoneal endometriosis.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Food Science & Technology
Borja Munoz-Solano, Elena Gonzalez-Penas
Summary: Mycotoxins are a major concern for food safety, as their exposure in animals can lead to health problems, economic losses, and contamination of animal-derived foods. This study used a revalidated methodology to analyze mycotoxins in animal plasma samples, finding that the prevalence of STER was high but levels were low in the analyzed feed. Animal biomonitoring can be a useful tool in assessing mycotoxin exposure, but more research is needed on appropriate biomarkers and analytical methods.
Article
Biochemistry & Molecular Biology
Claudia Homorogan, Diana Nitusca, Virgil Enatescu, Philip Schubart, Corina Moraru, Carmen Socaciu, Catalin Marian
Summary: Two putative metabolites were identified in plasma samples of MDD patients, suggesting potential diagnostic biomarkers. Additionally, another metabolite decreased significantly in the group following treatment.
Article
Microbiology
Maryam Mapar, Thomas Rydzak, Ryan A. Groves, Ian A. Lewis
Summary: Microbes have diverse metabolic capabilities and differences in these phenotypes are critical for differentiating strains, species, and broader taxa of microorganisms. Recent advances in liquid chromatography-mass spectrometry (LC-MS) allow researchers to track the complex combinations of molecules that are taken up by each cell type and to quantify the rates that individual metabolites enter or exit the cells. This metabolomics-based approach allows complex metabolic phenotypes to be captured in a single assay, enables computational models of microbial metabolism to be constructed, and can serve as a diagnostic approach for clinical microbiology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Guanxuanzi Zhang, Na Zhang, Liuhan Dong, Nan Bai, Yun Cai
Summary: A LC-MS/MS method for quantifying contezolid in plasma and cerebrospinal fluid (CSF) has been developed and verified in this study. Using linezolid as the internal standard, protein precipitation was performed. The LC-MS/MS analysis was performed using an Agilent EclipsePlus C18 column and acetonitrile and water mobile phases. The method showed good linearity, precision, and recovery rates in both plasma and CSF samples.
Article
Chemistry, Analytical
Dong Xiang, Tinghui Zhao, Jue Wang, Yang Cao, Qiuxia Yu, Lu Liu, Hengyi Yu, Xiping Li, Ninghong Li, Yang Yi, Xuepeng Gong
Summary: A sensitive and robust LC-MS/MS method was developed and validated for the quantification of olverembatinib in human plasma and cerebrospinal fluid (CSF). The method involved liquid-liquid extraction and precipitation enrichment for plasma and CSF pretreatment, respectively. The detection was performed by electrospray ionization in a positive ion mode using multiple reaction monitoring. The method proved to be accurate and precise, with a successful application in measuring olverembatinib concentrations in plasma and CSF from chronic myeloid leukemia patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Renmeng Liu, Xiaofeng Zhao, Guoju Geng, Yurong Lai
Summary: A simple and robust bioanalytical method was developed using LC-MS/MS to quantify kynurenic acid in human plasma. This method can serve as a tool readily available to assess potential drug-drug interactions mediated by inhibition of OAT1 and OAT3 activities.
Article
Chemistry, Analytical
Jiangshuo Li, Jing Xu, Ruiping Zhang, Jiuming He, Manjiangcuo Wang, Guanggen Jiao, Zeper Abliz
Summary: In this study, a targeted strategy for screening fatty acids was developed using parallel labeling and high-resolution mass spectrometry analysis. The method was successfully applied to determine fatty acid levels in plasma samples from esophageal squamous cell carcinoma patients and healthy controls, revealing six altered fatty acids affected by tumor invasion. This method provides insights into the identification of unknown fatty acids and has potential applications in clinical diagnosis and the study of disease mechanisms.
Article
Pharmacology & Pharmacy
Kevin McKinski, Dean McNulty, Francesca Zappacosta, Mary Birchler, Matt Szapacs, Christopher Evans
Summary: ICOS, expressed on the surface of activated T-cells, is being investigated as a potential therapeutic target. Recent studies found aberrant increases in sICOS in disease-state patients using commercial ELISA kits, but the results were not consistent with in-house immunoassays. The researchers developed a novel LC-MS/MS method to confirm the results and demonstrated that sICOS is a low abundance soluble protein.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Oncology
Xiangping Lin, Lucie Lecuyer, Xinyu Liu, Mohamed N. Triba, Melanie Deschasaux-Tanguy, Aicha Demidem, Zhicheng Liu, Tony Palama, Adrien Rossary, Marie-Paule Vasson, Serge Hercberg, Pilar Galan, Philippe Savarin, Guowang Xu, Mathilde Touvier
Summary: Correct identification of subjects at high risk is crucial for preventing and screening prostate cancer. Metabolite analysis in biofluids has shown potential in identifying novel biomarkers for PCa. The study found dysregulation of amino acids and sphingolipid metabolism to be associated with future risk of PCa, highlighting 10 specific metabolites.
Article
Biochemical Research Methods
Vincent R. Richard, Claudia Gaither, Robert Popp, Daria Chaplygina, Alexander Brzhozovskiy, Alexey Kononikhin, Yassene Mohammed, Rene P. Zahedi, Evgeny N. Nikolaev, Christoph H. Borchers
Summary: This study used multiplexed targeted mass spectrometry assays to determine the concentrations of hundreds of substances in the plasma of hospitalized COVID-19 patients. The researchers found clear distinctions between COVID-19 patients and controls, as well as significant differences between survivors and nonsurvivors. Using a machine learning model, they were able to predict patient survival with high accuracy based on the concentrations of a limited number of substances on the day of hospitalization.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Pharmacology & Pharmacy
Carmen Fernandez-Metzler, Brad Ackermann, Fabio Garofolo, Mark E. Arnold, Binodh DeSilva, Huidong Gu, Omar Laterza, Yan Mao, Mark Rose, Faye Vazvaei-Smith, Rick Steenwyk
Summary: This article discusses the guidance and challenges in validating biomarker assays for drugs and metabolites. It highlights the importance of reliable biomarker assays for regulatory submissions and offers specific recommendations for biomarker assay validation using LC-MS.
Article
Biochemistry & Molecular Biology
Stephanie J. Shiffka, Jace W. Jones, Linhao Li, Ann M. Farese, Thomas J. MacVittie, Hongbing Wang, Peter W. Swaan, Maureen A. Kane
JOURNAL OF LIPID RESEARCH
(2020)
Article
Cell & Tissue Engineering
Manasa P. Srikanth, Jace W. Jones, Maureen Kane, Ola Awad, Tea Soon Park, Elias T. Zambidis, Ricardo A. Feldman
Summary: Gaucher disease is caused by mutations in the GBA1 gene, leading to the accumulation of GlcSph which activates an mTORC1-dependent pathogenic mechanism in neurons. Inhibition of acid ceramidase can prevent mTOR hyperactivity and lysosomal dysfunction, suggesting a potential therapeutic target for GBA1-associated neurodegeneration. Incubation with exogenous GlcSph in wild-type neurons can recapitulate the abnormalities observed in mutant neurons, highlighting the role of GlcSph in the pathogenesis of Gaucher disease.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemical Research Methods
Anh Tran, I. Abrrey Monreal, Eugene Moskovets, Hector C. Aguilar, Jace W. Jones
Summary: An unmet need exists for analytical strategies that can characterize the lipid composition of viral envelopes and do so on a high-throughput scale. The use of atmospheric pressure MALDI with lithium adduct consolidation allows for rapid and confident profiling of total lipid extracts from enveloped viruses, specifically geared towards sphingolipid detection. This approach provides a robust and high-throughput lipid detection platform for structural characterization of lipid extracts from viruses.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Medicine, Research & Experimental
Ni Yang, Lauren E. Parker, Jianshi Yu, Jace W. Jones, Ting Liu, Kyriakos N. Papanicolaou, C. Conover Talbot, Kenneth B. Margulies, Brian O'Rourke, Maureen A. Kane, D. Brian Foster
Summary: The study showed that there is a decline in cardiac ATRA in heart failure, despite sufficient reserves of cardiac vitamin A. Strategies to normalize ATRA metabolism, such as CYP26 inhibition, may have therapeutic potential in preventing cardiac remodeling and functional decline. This suggests that low cardiac ATRA attenuates critical gene expression programs in heart failure.
Article
Chemistry, Analytical
Yulemni Morel, Nivedita Hegdekar, Chinmoy Sarkar, Marta M. Lipinski, Maureen A. Kane, Jace W. Jones
Summary: The study developed a targeted, quantitative method using HILIC separation and selective reaction monitoring mass spectrometry for specific and sensitive quantitation of 100 PE-P structures. Application of this assay to a TBI mouse model demonstrated distinct temporal profiles for plasma PE-P up to 28 days post injury, showing a significant increase 24 hours after induced TBI followed by a gradual reduction to sham concentrations by day 28.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Carballal, Victor Vitvitsky, Roshan Kumar, David A. Hanna, Marouane Libiad, Aditi Gupta, Jace W. Jones, Ruma Banerjee
Summary: This study demonstrates that H2S increases lipid synthesis in cells by affecting mitochondrial NAD(P)H pools and enabling reductive carboxylation of alpha-ketoglutarate. H2S also leads to time-dependent changes in various lipid classes, upregulating triglycerides while downregulating phosphatidylcholine.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Ludovic Muller, Weiliang Huang, Jace W. Jones, Ann M. Farese, Thomas J. MacVittie, Maureen A. Kane
Summary: Radiation exposure leads to dysregulation of lipid and protein composition in the mesenteric lymph node (MLN), affecting pathways regulating lipids and acute phase response. The study demonstrates molecular alterations in MLN after acute radiation exposure, potentially informing on injury mechanism in a non-human primate model of acute radiation syndrome of the gastrointestinal tract.
Article
Biochemistry & Molecular Biology
Owen B. Becette, Anh Tran, Jace W. Jones, John P. Marino, Robert G. Brinson
Summary: Nucleic acids are a popular platform for developing biotherapeutics. There are currently 14 short oligonucleotide therapeutics and 2 mRNA vaccines approved by the FDA. However, there is a lack of high-resolution structural techniques for characterizing these molecules. In this study, nuclear magnetic resonance (NMR) methods were used to structurally fingerprint short oligonucleotide therapeutics, demonstrating their utility and broad applicability.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Cell Biology
Jianshi Yu, Mariarita Perri, Jace W. Jones, Keely Pierzchalski, Natalia Ceaicovscaia, Erika Cione, Maureen A. Kane
Summary: The study investigated the relationship between RBP1 expression and atRA, finding that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Increasing atRA levels could activate direct atRA targets, inhibit proliferation, and collagen deposition. Conditions in tumor microenvironments reduced RBP1 expression and atRA, while treatments with specific agonists or agents could restore RBP1 expression and atRA levels. This work highlights the potential therapeutic benefit of restoring RBP1 expression and endogenous atRA in diseases with reduced RBP1.
Article
Substance Abuse
Bryan Mackowiak, Mingjiang Xu, Yuhong Lin, Yukun Guan, Wonhyo Seo, Ruixue Ren, Dechun Feng, Jace W. Jones, Hongbing Wang, Bin Gao
Summary: The study found that mice chronically fed with ethanol responded less severely to multiple binge ethanol consumption compared to a single binge. The upregulation of the Cyp2b10 gene after binge ethanol intake can indirectly modify ethanol metabolism and reduce acute-on-chronic ethanol-induced liver injury.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
Owen B. Becette, Anh Tran, John P. Marino, Jace W. Jones, Robert G. Brinson
Summary: In this study, a simplified and quick method for identification and assessment of a known impurity in nucleic acid drugs was developed using lithium adduct consolidation combined with mass spectrometry and nuclear magnetic resonance spectroscopy. The method is believed to have broad applicability in academic and industrial laboratories.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Genetics & Heredity
Clorissa L. Washington-Hughes, Shubhrajit Roy, Herana Kamal Seneviratne, Senthilkumar S. Karuppagounder, Yulemni Morel, Jace W. Jones, Alex Zak, Tong Xiao, Tatiana N. Boronina, Robert N. Cole, Namandje N. Bumpus, Christopher J. Chang, Ted M. Dawson, Svetlana Lutsenko
Summary: This study reveals the importance of Cu transporter Atp7b in maintaining Cu homeostasis in the brain during postnatal development. Loss of Atp7b function leads to morphological changes in choroid plexus and affects Cu transporters Atp7a and Slc31a, resulting in Cu deficit and associated imbalance of catecholamines and lipids. These findings contribute to our understanding of the neuropathology of Wilson disease.
Article
Biochemical Research Methods
Yulemni Morel, Jace W. Jones
Summary: Lipid peroxidation is crucial in the development of various diseases, as it causes oxidative damage to lipids, leading to dysfunction and death of cells. This study presents an analytical approach for characterizing the structure of oxidized products of specific types of phospholipids, providing insights into the initial impact of lipid peroxidation on cellular membranes and their role in redox biology.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Stephanie Zalesak-Kravec, Weiliang Huang, Jace W. Jones, Jianshi Yu, Jenna Alloush, Amy E. Defnet, Alexander R. Moise, Maureen A. Kane
Summary: This study investigated the impact of CRBP1 loss on the adult mouse heart using a multi-omics approach. The results showed that RA levels were decreased in Rbp1(-/-) mouse heart tissue, and metabolomics and proteomics analyses revealed disrupted pathways related to cellular metabolism and cardiac metabolism. These findings characterize the effect of CRBP1 loss and reduced RA in the adult heart.
Article
Biochemical Research Methods
Anh Tran, Liting Wan, Zhenbo Xu, Janette M. Haro, Bing Li, Jace W. Jones
Summary: The research team utilized lithium adduct consolidation to enhance mass-spectrometry-based sphingolipid analysis and implemented an optimized MALDI matrix system for high-throughput detection. They successfully applied the technology to detect differentially expressed sphingolipids in liver, brain, and plasma matrices, validating the method's efficacy.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)