Article
Biochemical Research Methods
Edvaldo Vasconcelos Soares Maciel, Fernando Mauro Lancas
Summary: This study presents a method that combines solid-packed cartridges and capillary LC-MS to integrate sample preparation and analytical steps. The proposed method effectively extracts and analyzes selected pesticides in soil samples, with potential applications in other solid samples. Initial qualitative tests on pesticides in honeybees were also conducted.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Guangshi Chen, Caiming Tang, Jianhua Tan, Zewen Zhu, Shang Guo, Jing Zhou, Xianzhi Peng
Summary: A reliable and sensitive analyzing method was developed and validated for the determination of 13 novel bisphenol analogues and bisphenol A in organism tissues. The method successfully determined the content of these substances through a series of extraction and purification steps, and was applied to a preliminary investigation in wild samples. This research provides a foundation for further understanding the accumulation of these novel bisphenol analogues in the environment.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Evan Lesne, Miguel Munoz-Bartual, Francesc A. Esteve-Turrillas
Summary: A fast and simple procedure based on MEPS and LC-MS/MS was developed for quantifying 28 synthetic hallucinogens in oral fluids. The proposed method demonstrated high precision and quantitative recoveries, with limits of detection ranging from 0.09 to 1.22 µg/L. It is suitable for the determination of NBOMe derivatives and other synthetic hallucinogenic substances in oral fluid samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mahboube Shirani, Abolfazl Aslani, Soheila Sepahi, Ehsan Parandi, Azadeh Motamedi, Elham Jahanmard, Hamid Rashidi Nodeh, Behrouz Akbari-adergani
Summary: In this study, a three-dimensional adsorbent GO/AgO@Ni foam was developed for rapid and efficient vortex assisted floating solid phase extraction of bisphenol A in canned food products. The synthesized GO/AgO@Ni foam was characterized using SEM, EDX, and FT-IR techniques. The effects of various factors on the extraction efficiency of bisphenol A were explored. The method showed good figures of merit and achieved satisfactory precision and recovery for bisphenol A in canned food samples.
ANALYTICAL METHODS
(2022)
Article
Food Science & Technology
Marica Erminia Schiano, Federica Sodano, Chiara Cassiano, Ferdinando Fiorino, Serenella Seccia, Maria Grazia Rimoli, Stefania Albrizio
Summary: The consumption of plant-based beverages as an alternative to cow's milk has gained attention worldwide. This study focused on monitoring the intake of BPA, BPB, and BPS in the Italian population through the consumption of these beverages. Analysis of popular brands in Italy found BPA and BPB in the samples. Risk assessment showed that if the new temporary TDI value is confirmed, more attention would be needed to protect consumer health.
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Teresa Chioccarelli, Marina Migliaccio, Antonio Suglia, Francesco Manfrevola, Veronica Porreca, Nadia Diano, Sonia Errico, Silvia Fasano, Gilda Cobellis
Summary: The study demonstrates that early exposure to BPA can lead to sustained and site-specific accumulation of BPA in epididymal fat in adulthood, becoming a risk factor for reproductive endocrine pathways associated with ECS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Giada Frenzilli, Joan Martorell-Ribera, Margherita Bernardeschi, Vittoria Scarcelli, Elisabeth Jonsson, Nadia Diano, Martina Moggio, Patrizia Guidi, Joachim Sturve, Noomi Asker
Summary: The study shows that both bisphenol A and its alternative bisphenol S can cause endocrine disrupting and genotoxic effects in brown trout, indicating two different mechanisms of damage underlying the activities of bisphenol A and bisphenol S. Bisphenol A primarily increases micronuclei frequency in fish erythrocytes, while bisphenol S may cause damage through other mechanisms.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Food Science & Technology
Pasquale Gallo, Samantha Imbimbo, Silvana Alvino, Vincenzo Castellano, Olga Arace, Vittorio Soprano, Mauro Esposito, Francesco Paolo Serpe, Donato Sansone
Summary: This study presents the monitoring results of aflatoxins B/G in food of vegetal origin imported in Southern Italy from extra-European Union countries, revealing contamination in a significant number of samples and highlighting nuts as the most susceptible food products. Specifically, pistachio nuts, hazelnuts, and almonds were identified as the major sources of exposure for consumers. Additional attention is needed for products like chili pepper and Brazil nuts to understand their contamination levels.
Article
Microbiology
Raffaella Parente, Valentina Possetti, Maria Lucia Schiavone, Elisabetta Campodoni, Ciro Menale, Mattia Loppini, Andrea Doni, Barbara Bottazzi, Alberto Mantovani, Monica Sandri, Anna Tampieri, Cristina Sobacchi, Antonio Inforzato
Summary: Osteomyelitis is an infectious disease of the bone caused by Staphylococcus aureus, and a new 3D model has been developed to better understand the pathogenetic mechanisms of the disease.
Review
Chemistry, Analytical
Ines Delfino, Nadia Diano, Maria Lepore
Summary: This paper discusses the dangers of phenolic compounds in the environment and the drawbacks of conventional detection methods, as well as the interest in developing new sensing methods for rapid and easy monitoring of phenolic compound concentrations.
Article
Agriculture, Dairy & Animal Science
Ilaria Di Marco Pisciottano, Grazia Guadagnuolo, Fabio Busico, Luca Alessandroni, Bruno Neri, Domenico Vecchio, Gabriele Di Vuolo, Giovanna Cappelli, Alessandra Martucciello, Pasquale Gallo
Summary: The restriction on the use of BPA has led manufacturers to use analogues, which have been proven to have endocrine-disrupting activity and are already widespread in the environment. The intake of these chemicals through diet is a public concern, but there is limited data on food contamination. This study investigated the levels of BPA and its analogues in the production chain of buffalo milk and found that BPA was the most abundant contaminant.
Correction
Endocrinology & Metabolism
Eleonora Palagano, Mary A. Slatter, Paolo Uva, Ciro Menale, Anna Villa, Mario Abinun, Cristina Sobacchi
Article
Biochemistry & Molecular Biology
Ciro Menale, Gaia Tabacco, Anda Mihaela Naciu, Maria Lucia Schiavone, Francesca Cannata, Emanuela Morenghi, Cristina Sobacchi, Andrea Palermo
Summary: The study aimed to assess the association between Dpp3 activity and bone fragility in postmenopausal osteoporosis, as well as the impact of denosumab on the enzymatic activity. The results showed that serum Dpp3 activity was significantly reduced in patients with severe osteoporosis compared to controls and was associated with bone mass. Additionally, there was a negative correlation between Dpp3 activity and specific bone metabolism markers. Dpp3 activity did not change after denosumab injection.
Article
Cell Biology
Giulia Ricci, Florian Guillou, Angela Catizone, Vincenza Grazia Mele, Martina Moggio, Teresa Chioccarelli, Nadia Diano, Rosaria Meccariello, Riccardo Pierantoni, Silvia Fasano, Gilda Cobellis, Rosanna Chianese, Francesco Manfrevola
Summary: This study reveals a novel role for Kisspeptin-10 in Leydig cells, where it induces the cytoskeletal-nucleoskeletal pathway through physical interaction with KISS1 receptor and ANKRD31, resulting in gene expression activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Immunology
Konstantin Horas, Ciro Menale, Antonio Maurizi
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sonia Errico, Martina Moggio, Nadia Diano, Marianna Portaccio, Maria Lepore
Summary: Fourier transform infrared (FTIR) spectroscopy is a powerful tool for analyzing biological samples and has been widely used in biomedical and biotechnological research. This paper describes the procedures for preparing and analyzing different types of samples using a commercial FTIR spectrometer, demonstrating its versatility and potentiality.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Gina Cavaliere, Angela Catapano, Giovanna Trinchese, Fabiano Cimmino, Ciro Menale, Lidia Petrella, Maria Pina Mollica
Summary: Obesity is a prevalent disorder caused by an imbalance between energy consumption and metabolizable energy intake. Aging further disrupts this balance and impairs the mechanisms controlling energy homeostasis. It results in excessive fat deposition, systemic inflammation, higher levels of free fatty acids, and ectopic lipid accumulation in adipocytes. Chronic liver disease is often associated with hepatic fat accumulation, which is characterized by mitochondrial dysfunction, impaired fat metabolism, and increased oxidative stress. This study investigates the interaction between adipose tissue and the liver, focusing on changes in hepatic mitochondrial fatty acid oxidation and storage during high-fat diet-induced obesity in a rodent model. The results demonstrate that both obesity and aging lead to dysregulation of adipose tissue function, metabolic alterations, and mitochondrial dysfunction, with aging exacerbating liver mitochondrial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Benedetta Talone, Arianna Bresci, Francesco Manetti, Federico Vernuccio, Alejandro De La Cadena, Chiara Ceconello, Maria Lucia Schiavone, Stefano Mantero, Ciro Menale, Renzo Vanna, Giulio Cerullo, Cristina Sobacchi, Dario Polli
Summary: Bone tissue has a complex microarchitecture and biomolecular composition, which play a crucial role in determining its biomechanical properties. By utilizing multimodal nonlinear optical microscopy, it is possible to accurately characterize and compare normal and pathological bone tissue at biochemical, morphological, and topological levels. This technique has the potential to be applied in preclinical studies and even clinical practice for the analysis of larger tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nutrition & Dietetics
Ciro Menale, Giovanna Trinchese, Immacolata Aiello, Giulia Scalia, Monica Dentice, Maria Pina Mollica, Nal Ae Yoon, Sabrina Diano
Summary: In this study, it was found that the addition of non-lipotoxic levels of 25 μM palmitic acid (PA) to glucose (G) increased mineralization in osteoblasts (OBs). It was also discovered that G+25 μM PA reduced mitochondria size in OBs and increased mitochondria respiration and ATP production, as well as the expression of oxidative phosphorylation genes. Treatment with the mitochondrial fission inhibitor Mdivi-1 reduced osteogenesis and mitochondrial respiration in OBs.
Article
Materials Science, Biomaterials
Annalisa La Gatta, Virginia Tirino, Marcella Cammarota, Marcella La Noce, Antonietta Stellavato, Anna Virginia Adriana Pirozzi, Marianna Portaccio, Nadia Diano, Luigi Laino, Gianpaolo Papaccio, Chiara Schiraldi
Summary: This study evaluated for the first time the combination of enzymatically crosslinked gelatin with hyaluronan and biotechnological chondroitin in enhancing osteogenic potential. The results showed that the combination improved the expression of osteogenic proteins and accelerated the formation of calcified matrix, indicating a promising approach for fastening bone deposition.
REGENERATIVE BIOMATERIALS
(2021)