Article
Chemistry, Medicinal
Abdulaziz A. Aldubayyan, Erika Castrignano, Simon Elliott, Vincenzo Abbate
Summary: This study presents a validated LC-MS/MS method for detecting and quantifying synthetic cathinones and their metabolites in human urine. Analysis of suspicious urine samples from Saudi Arabia revealed the presence of synthetic cathinones, specifically mephedrone and N-ethylpentylone, as well as their dihydro-metabolites, along with other classical stimulant drugs. This is the first report of new psychoactive substance use in Saudi Arabia involving designer stimulant drugs. The findings highlight the need for broader urine analysis to detect and report otherwise undetected or new analytes.
Article
Pharmacology & Pharmacy
N. La Maida, G. Mannocchi, S. Pichini, G. Basile, A. Di Giorgi, F. P. Busardo, E. Marchei
Summary: A targeted screening and quantification method for synthetic cathinones (SCs) in hair samples was developed and validated. The method showed good selectivity, specificity, and easy and low-cost sample preparation. The most prevalent SCs in hair samples from SCs consumers were 3,4-Methylenedioxy-alpha-Pyrrolidinohexanophenone, alpha-Pyrrolidinohexiophenone, 3-Methylmethcathinone, and 4-Methylethcathinone.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Yan Shi, Mengxi Liu, Xiangjun Li, Niusheng Xu, Shuai Yuan, Zhiguo Yu, Ping Xiang, Hejian Wu
Summary: Synthetic cannabinoids are a group of new psychoactive substances that can cause various symptoms and even death when abused. An efficient liquid chromatography-high resolution mass spectrometry method is crucial for their identification and analysis.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Medicine, Legal
Laura Franke, Frank T. Peters, Dirk K. Wissenbach
Summary: The study aimed to analyze small endogenous biomolecules and investigate their long-term stability in urine samples. The results showed that most of the biomolecules were stable on various surfaces and under different storage conditions, except for kynurenic acid. The study demonstrated the value of sensitive analysis methods, but further research is needed for accurate body fluid identification.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Medical Laboratory Technology
Ju-Yu Chen, Guan-Yuan Chen, Hooi-Nee Ong, Mei-Ling Lai, Yi-Ju Ho, Ching-Hua Kuo, Te- Weng
Summary: This study found that blood collection tubes have a significant impact on the recoveries of synthetic cathinones, especially serum separating tubes and gel-containing tubes. However, in real-world scenarios, nonideal collection methods are inevitably employed, so it is important to rigorously interpret forensic toxicological results, especially for susceptible species.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Chiara Lavarello, Sebastiano Barco, Martina Bartolucci, Isabella Panfoli, Emanuele Magi, Gino Tripodi, Andrea Petretto, Giuliana Cangemi
Summary: LC-HRMS is the preferred method for untargeted metabolomic analysis, with established protocols to ensure accurate metabolite identification. The study successfully built an AMRT database using a commercial metabolite library, achieving level 1 identification of metabolites in pediatric plasma and urine samples, providing a foundation for future metabolomic studies in pediatric settings.
Article
Toxicology
Selina Hemmer, Lea Wagmann, Markus R. Meyer
Summary: The study utilized untargeted metabolomics to investigate the acute effects of amphetamine on the plasma and urinary metabolome in rats. It revealed a downregulation of amino acids in plasma samples after amphetamine exposure, and identified four new potential biomarkers in urine. The research highlights the need for further studies to elucidate altered metabolic pathways associated with acute amphetamine exposure.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Selina Hemmer, Lea Wagmann, Benedikt Pulver, Folker Westphal, Markus R. Meyer
Summary: This study investigated the effects of synthetic cathinones on in vitro and in vivo metabolomes, and identified relevant endogenous and exogenous biomarkers. The findings are important for establishing analytical screening procedures and understanding the physiological changes associated with cathinone abuse.
Article
Chemistry, Analytical
Cristian Suarez-Oubina, Pamela Cabarcos-Fernandez, Maria Jesus Tabernero-Duque, Pilar Bermejo-Barrera, Antonio Moreda-Pineiro, Ana Maria Bermejo-Barrera
Summary: Using ultrasounds, the extraction time for membrane-assisted solvent extraction (MASE) in isolating synthetic cathinones from urine has been shortened to 30 minutes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for the separation and determination of eleven synthetic cathinones. The developed method showed efficient extraction and pre-concentration of synthetic cathinones with LogP values higher than 2.0.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Ricardo Leal Cunha, Celinalva da Silva Lima Oliveira, Aline Lima de Oliveira, Adriano Otavio Maldaner, Pedro Afonso P. Pereira
Summary: Stimulants are commonly abused drugs that act on the central nervous system, with a large number of users worldwide. In northeast Brazil, amphetamine-type stimulants and synthetic cathinones are the main synthetic drugs seized recently. These substances are used in popular festivals and rave parties, but also in cases of drug-facilitated sexual assault and by truck drivers.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Antonia Mazzeo, Michela Varra, Luciana Tartaglione, Patrizia Ciminiello, Zita Zendong, Philipp Hess, Carmela Dell'Aversano
Summary: Palytoxin and its congeners, despite structural similarities, exhibit different toxic potencies, necessitating their individual isolation for toxicological and analytical purposes. Evaporation was identified as a critical step that significantly affected the overall recovery of PLTX congeners during large-scale preparative work. Experiments demonstrated that recoveries strongly depended on solubility and the material of the container used, with successful recovery achieved through specific drying techniques.
Article
Chemistry, Analytical
Andrea Cerrato, Carmen Bedia, Anna Laura Capriotti, Chiara Cavaliere, Vincenzo Gentile, Martina Maggi, Carmela Maria Montone, Susy Piovesana, Alessandro Sciarra, Roma Tauler, Aldo Lagan
Summary: The study focused on analyzing polar and positively charged metabolites in urine samples from benign prostatic hyperplasia and prostatic cancer patients, utilizing an analytical platform with up to 40-fold analyte enrichment. By applying the ROIMCR procedure to complex metabolomics datasets generated by UHPLC-HRMS, differential metabolites were successfully resolved and tentatively identified, underscoring the importance of this approach in cancer biomarker research. The study highlights the significance of previously neglected metabolite classes in cancer biomarker discovery and the value of the proposed methodology.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Alice Ameline, Laurie Gheddar, Jean-Sebastien Raul, Pascal Kintz
Summary: Selective androgen receptor modulator S-23 is a relatively new anabolic agent in the doping market, and little is known about its long-term effects. This study aimed to detect S-23 and its metabolites in human urine using advanced analytical techniques, and identified four metabolites in vitro. Although only one metabolite was detected in urine, this study provides valuable insights for future research on S-23.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Endocrinology & Metabolism
Salah Abdelrazig, Catharine A. Ortori, Michael Doherty, Ana M. Valdes, Victoria Chapman, David A. Barrett
Summary: Metabolomics studies have identified potential surrogate biomarkers of knee osteoarthritis in urine, aiding in a better understanding of OA progression and potential targeted treatment.
Article
Biochemical Research Methods
S. Lefeuvre, J. Bois-Maublanc, E. Mongeois, V. Policarpo, L. Formaux, T. Francia, E. M. Billaud, L. Got
Summary: A new urinary catecholamine detection method was developed and validated for pheochromocytoma, paraganglioma and neuroblastoma. It has been successfully applied in clinical settings with good correlation and stability.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Toxicology
Jennifer Hanna, Elena Lendoiro, Ana de Castro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Angelines Cruz-Landeira, Manuel Lopez-Rivadulla, Marta Concheiro-Guisan
FORENSIC TOXICOLOGY
(2019)
Article
Chemistry, Analytical
Victoria Mei, Marta Concheiro, Justine Pardi, Gail Cooper
JOURNAL OF ANALYTICAL TOXICOLOGY
(2019)
Article
Chemistry, Analytical
A. Lopez-Rabunal, E. Lendoiro, M. Concheiro, M. Lopez-Rivadulla, A. Cruz, A. de-Castro-Rios
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Angela Lopez-Rabunal, Elena Lendoiro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Marta Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Manuel Lopez-Rivadulla, Angelines Cruz, Ana de-Castro-Rios
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Timothy Lau, Marta Concheiro, Gail Cooper
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Natalia A. Platosz, Tina M. Binz, Markus R. Baumgartner, Elena Lendoiro, Ana de Castro, Marta Concheiro
Summary: The use and misuse of synthetic and prescription opioids in the USA has become an epidemic, leading to a high number of deaths and cases of opioid use disorder. Hair is now being increasingly used in forensic toxicology to investigate drug use patterns due to its long window of detection. A method was developed to detect and quantify 27 classic, prescription, and synthetic opioids in hair using LC-MS-MS.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2021)
Article
Medicine, Legal
Steven Towler, Marta Concheiro, Sue Pearring, Luke N. Rodda
Summary: Performing point-of-care urine drug screen testing at autopsy by a forensic pathologist may offer early indication of presence of analytes; Evaluation of Alere iCup DX 14 for screening postmortem urine drug abuse; Sensitivity and specificity of iCup for various drug classes in comparison with confirmatory tests.
JOURNAL OF FORENSIC SCIENCES
(2021)
Article
Neurosciences
Britta Hahn, Alexander N. Harvey, Marta Concheiro-Guisan, Marilyn A. Huestis, Thomas J. Ross, Elliot A. Stein
Summary: This study found that nAChR antagonist can weaken task-induced deactivation of the DMN, indicating that the constant activation tone of nAChR typically helps regulate DMN activity in healthy individuals. This implies that low nAChR tone may play a causal role in DMN dysregulation seen in conditions such as mild cognitive impairment or Alzheimer's disease.
PSYCHOPHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Marta Concheiro, Frank Martin Gutierrez, Alejandro Ocampo, Elena Lendoiro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Angelines Cruz-Landeira, Manuel Lopez-Rivadulla, Ana de-Castro-Rios
Summary: Analyzing prenatal marijuana exposure in paired hair, meconium, umbilical cord, and placental samples revealed complementarity between meconium and hair, with higher agreement with meconium for umbilical cord and placental results. THCCOOH-glucuronide in umbilical cord and placenta can be a biomarker of cannabis exposure, offering a potential alternative to meconium.
DRUG TESTING AND ANALYSIS
(2021)
Article
Toxicology
Angela Lopez-Rabunal, Elena Lendoiro, Marta Concheiro-Guisan, Eva Gonzalez-Colmenero, Patricia Penas-Silva, Ana Concheiro-Guisan, Manuel Macia-Cortinas, Manuel Lopez-Rivadulla, Ana De-Castro-Rios, Angelines Cruz
Summary: This study compared meconium, maternal hair, and maternal medical records to detect exposure to antidepressants and benzodiazepines during pregnancy. Maternal hair was more sensitive in detecting antidepressant use, while meconium analysis was better at providing detection of neonatal benzodiazepines exposure at delivery. No significant influence of prenatal exposure to these drugs on neonatal outcomes was observed.
FORENSIC TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Laura DeFreitas, Ana Miguel Fonseca Pego, Robert Kronstrand, Elena Lendoiro, Ana De Castro-Rios, Marta Concheiro
Summary: Identification and analysis of designer benzodiazepines have become a challenge in forensic toxicology. A fast and sensitive method using LC-MS/MS was developed and validated to determine 17 designer benzodiazepines in hair samples.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Article
Substance Abuse
Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Marta Lorenzo-Martinez, Marta Concheiro, Elena Lendoiro, Ana de-Castro-Rios, Debora Canizo-Vazquez, Cristina Martinez-Reglero, Jose Ramon Fernandez-Lorenzo
JOURNAL OF ADDICTIVE DISEASES
(2020)
Article
Medicine, Legal
Nicole Centazzo, Bonnie-Marie Frederick, Alethea Jacox, Shu-Yuan Cheng, Marta Concheiro-Guisan
FORENSIC SCIENCES RESEARCH
(2019)
Article
Genetics & Heredity
Tebah Browne, Marta Concheiro-Guisan, Mechthild Prinz
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
(2019)
Proceedings Paper
Chemistry, Applied
Nicole Centazzo, Marta Concheiro-Guisan
WASTEWATER-BASED EPIDEMIOLOGY: ESTIMATION OF COMMUNITY CONSUMPTION OF DRUGS AND DIETS
(2019)