Article
Immunology
Beatrix Paton, Pol Herrero, Joaquim Peraire, Antoni del Pino, Silvia Chafino, Javier Martinez-Picado, Frederic Gomez-Bertomeu, Anna Rull, Nuria Canela, Manuel Suarez
Summary: The N-glycosylation profiles in plasma of COVID-19 patients vary according to disease severity, with certain N-glycans showing potential as biomarkers for risk stratification and prognosis assessment. These findings indicate the potential of glycans as biomarkers for COVID-19 severity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Obstetrics & Gynecology
See Ling Loy, Jieliang Zhou, Liang Cui, Tse Yeun Tan, Tat Xin Ee, Bernard Su Min Chern, Jerry Kok Yen Chan, Yie Hou Lee
Summary: The study identified potential biomarkers for peritoneal endometriosis in peritoneal fluid and serum using metabolomics analysis. Phenylalanyl-isoleucine showed promising discriminatory performance in distinguishing peritoneal endometriosis from controls, particularly in the proliferative phase. Further research is needed to validate these findings and assess the clinical utility of phenylalanyl-isoleucine as a diagnostic biomarker for peritoneal endometriosis.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Medicine, General & Internal
Hyebin Choi, Sora Mun, Eun-Jeong Joo, Kyu Young Lee, Hee-Gyoo Kang, Jiyeong Lee
Summary: A proteomic study identified prothrombin as a potential biomarker for MDD, showing significantly increased levels in depressive patients and its association with platelet hyper-activation. This suggests prothrombin could be used for screening, prognosis, and diagnosis of MDD.
Article
Pharmacology & Pharmacy
Katharina Rox, Silke Rath, Dietmar H. Pieper, Marius Vital, Mark Broenstrup
Summary: A fast and cost-effective method based on liquid chromatography-tandem mass spectrometry that does not require isotope-labeled internal standards was reported for accurate quantification of TMAO and its precursors in real human plasma samples. By using an artificial plasma matrix for calibration, the analytical problem posed by the presence of endogenous TMAO and its precursors was circumvented, facilitating the establishment of TMAO as a routine clinical biomarker.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Byeong Gwan Cho, Peilin Jiang, Mona Goli, Sakshi Gautam, Yehia Mechref
Summary: This study investigated the viability of a newly commercialized micro array pillar nanoLC columns (mu PAC) for the analysis of permethylated glycans, demonstrating its efficient separation of different types of glycans and multiple advantages.
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Byeong Gwan Cho, Cristian D. Gutierrez Reyes, Mona Goli, Sakshi Gautam, Alireza Banazadeh, Yehia Mechref
Summary: Targeted mass spectrometric analysis, including techniques like selected reaction monitoring and multiple reaction monitoring, has been widely used in structural analysis of biomolecules. The recent innovation of parallel reaction monitoring (PRM) allows simultaneous detection of all product ions, simplifying the workflow and improving data quality. PRM offers advantages in reducing background interference and improving sensitivity, attributed to developments in sensitive mass analyzers like orbitrap and time of flight.
ANALYTICAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Stefania Noerman, Jyrki K. Virtanen, Marko Lehtonen, Carl Brunius, Kati Hanhineva
Summary: The study aimed to identify fasting serum metabolites associated with whole grain intake in a free-living population. The findings suggested strong associations between whole grain intake and several metabolites, which remained significant even after adjusting for potential confounders. These metabolites have the potential to serve as robust biomarkers for whole grain consumption.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Review
Biochemistry & Molecular Biology
Weifu Ren, Qi Bian, Yan Cai
Summary: Kidney disease is a global health concern that requires upgraded diagnostic tools to diagnose and prevent disease progression at an early stage. N-glycosylation, as an important protein modification, plays a crucial role in renal structure and function. Research on the role of N-glycosylation in kidney disease provides insights into its pathophysiology and paves the way for clinical applications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Analytical
You-Rim Lee, Seungyeon Lee, Sohyen Kwon, Jiyeong Lee, Hee-Gyoo Kang
Summary: Metabolomic research has been conducted in various fields, but the combination of forensic science and metabolomics is rare. This study identified and validated metabolite markers for determining the age of bloodstains within a week post-bleeding. These markers could be used as innovative tools for reconstructing crime scenes and suggesting initial investigative direction.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Kevin McKinski, Dean McNulty, Francesca Zappacosta, Mary Birchler, Matt Szapacs, Christopher Evans
Summary: ICOS, expressed on the surface of activated T-cells, is being investigated as a potential therapeutic target. Recent studies found aberrant increases in sICOS in disease-state patients using commercial ELISA kits, but the results were not consistent with in-house immunoassays. The researchers developed a novel LC-MS/MS method to confirm the results and demonstrated that sICOS is a low abundance soluble protein.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Karen E. Butler, Jaclyn Gowen Kalmar, David C. Muddiman, Erin S. Baker
Summary: Glycosylation is a common protein modification associated with various diseases, causing challenges in understanding the relationship between glycans and biological functions. The combination of IMS and MS technologies with the INLIGHT(TM) strategy enhances glycan identification confidence and accelerates the identification process in complex biological samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Donghui Lao, Rong Liu, Jianying Liang
Summary: By applying metabolomics, potential biomarkers for AIDS patients have been identified and verified, suggesting their potential role in the diagnosis of HIV/AIDS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Chi Soo Park, Minju Kang, Ahyeon Kim, Chulmin Moon, Mirae Kim, Jieun Kim, Subin Yang, Leeseul Jang, Ji Yeon Jang, Ha Hyung Kim
Summary: This study successfully distinguished sialylated N-glycan linkage isomers with a2-3 or a2-6 connections in the therapeutic glycoprotein CTLA4-Ig using liquid chromatography-tandem mass spectrometry. The isomers were identified and quantified by comparing the intensity of N-acetylglucosamine ion to sialic acid ion and the retention time-shift for a selective m/z value. The study also revealed the proportions and structures of different linkage isomers.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Sandra Taylor, Matthew Ponzini, Machelle Wilson, Kyoungmi Kim
Summary: Missing values are common in high-throughput mass spectrometry data. Two strategies are available to address missing values: imputation and imputation-free methods. This study reviews the impact of sample size and percentage of missing values on statistical inference, comparing the performance of different methods.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Clinical Neurology
Sundeep Tumber, Adam Bacon, Casey Stondell, Sampaguita Tafoya, Sandra L. Taylor, Yashar Javidan, Eric Klineberg, Rolando Roberto
Summary: This retrospective study compared high- versus low-dose tranexamic acid (TXA) in reducing blood loss during surgery for adolescent idiopathic scoliosis (AIS). Results showed that high-dose TXA group had significantly lower blood loss, total estimated blood loss, and transfusion need compared to low-dose TXA group. These findings suggest that high-dose TXA may be more beneficial in reducing blood loss for AIS patients undergoing posterior spine fusion surgery when combined with other Patient Blood Management strategies.
Article
Hematology
Mirjam Kruijt, Liselotte M. van der Pol, Jeroen Eikenboom, Harjo J. Verburg, Christa M. Cobbaert, L. Renee Ruhaak
Summary: This case study highlights the importance of using mass spectrometry to detect antithrombin deficiency, which identified a mutation associated with increased risk of thrombosis. It underscores the limitations of traditional diagnostics in potentially missing diagnoses.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Letter
Dermatology
Emanual Maverakis, William Liakos, Dayoung Park, Forum Patel, Fariha Siddiqui, Muchena J. Kailemia, L. Renee Ruhaak, Alina I. Marusina, Guillaume Luxardi, Johann E. Gudjonsson, Stephanie T. Le, April W. Armstrong, Wilson Liao, Alexander A. Merleev, Carlito B. Lebrilla
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Medical Laboratory Technology
Tirsa Teresa van Duijl, Renee Ruhaak, Ellen Hoogeveen, Renee de Mutsert, Frits Rosendaal, Saskia le Cessie, Johan de Fijter, Christa Cobbaert
Summary: This study establishes urinary reference intervals of kidney injury biomarkers in a middle-aged Dutch population, and identifies NGAL, IGFBP7, TIMP2, KIM-1, and uromodulin as quantifiable biomarkers. Urine creatinine and osmolality are correlated to urine volume, IGFBP7, and TIMP2 concentrations.
ANNALS OF CLINICAL BIOCHEMISTRY
(2022)
Article
Medical Laboratory Technology
Nico P. M. Smit, Fred P. H. T. M. Romijn, Vanessa J. J. van Ham, Esther Reijnders, Christa M. Cobbaert, L. Renee Ruhaak
Summary: Plasma anticoagulants affect the results of quantitative protein mass-spectrometry (QPMS) analysis of apolipoproteins. Heparin plasma shows comparable results to serum, whereas citrate plasma shows reduced concentrations that can be explained by dilution. EDTA-plasma shows reduced recovery due to altered proteolytic digestion efficiency.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Frank K. Liou, Patrick Y. Kim, S. Paran Yap, Abdullah Khan, Sandra Taylor, Rex Pillai, Eric King, Amol Shah, R. Torrance Andrews, Catherine T. Vu, Roger E. Goldman
Summary: This study evaluated the short- and long-term outcomes of implantable port catheters (IPCs) inserted via alternate cervical veins (ACV) compared to traditionally inserted IPCs via the internal jugular vein (IJV). Results showed that ACV access was non-inferior to traditional access for overall complications and had fewer complications compared to traditional access.
CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY
(2023)
Article
Medical Laboratory Technology
L. Renee Ruhaak, Fred P. H. T. M. Romijn, Ilijana Begcevic Brkovic, Zsusanna Kuklenyik, Julia Dittrich, Uta Ceglarek, Andrew N. Hoofnagle, Harald Althaus, Eduardo Angles-Cano, Stefan Coassin, Vincent Delatour, Liesbet Deprez, Ioannis Dikaios, Gerhard M. Kostner, Florian Kronenberg, Alicia Lyle, Urban Prinzing, Hubert W. Vesper, Christa M. Cobbaert, Int Federation Clinical Chem Lab Med Working Grp Apolipoproteins Mass Spect
Summary: In order to ensure the accuracy and reliability of medical results from in vitro diagnostic and in-house tests, a higher order reference measurement system traceable to endorsed reference measurement procedures and reference materials is needed. A candidate reference measurement procedure for serum apolipoprotein(a) [apo(a)] based on mass spectrometry was developed and provisionally validated. The method demonstrated accurate quantitation of serum apo(a) and fulfilled predefined analytical performance specifications.
CLINICAL CHEMISTRY
(2023)
Review
Medical Laboratory Technology
Nina M. M. Diederiks, Yuri E. M. van der Burgt, L. Renee Ruhaak, Christa M. M. Cobbaert
Summary: In the past decade, there has been a significant resurgence of interest in serum/plasma lipoprotein(a) (Lp(a)) as an independent risk factor for cardiovascular disease (CVD). Updated evidence has been published supporting a causal association between Lp(a) concentrations and cardiovascular outcomes in different ethnic groups. The development of new Lp(a)-lowering drugs has also contributed to the growing interest in measuring Lp(a) levels. The accuracy and standardization of Lp(a) tests are crucial for identifying high-risk individuals and evaluating the effectiveness of Lp(a)-lowering medication.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Article
Veterinary Sciences
Tarini V. Ullal, Stanley L. Marks, Sara N. Huebner, Sandra L. Taylor, Courtney D. Shelley
Summary: Serum folate is considered a biomarker of chronic enteropathy (CE) in dogs, and this study aimed to examine its associations with markers of CE. The results showed that hypofolatemia was more common in dogs with CE and was associated with hypocobalaminemia, higher CIBDAI, and hypoalbuminemia.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Letter
Anesthesiology
Naileshni Singh, Michael J. Jung, Fatima Yusuf, Almas Khan, Tiffany Su, Allison Glinka-Przybysz, Sandra L. Taylor
Article
Hematology
Mirjam Kruijt, Maxim M. Treep, Christa M. Cobbaert, L. Renee Ruhaak
Summary: This study developed and analytically validated a mass spectrometry-based test for molecular characterization of antithrombin. The test showed good correlation with a commercial activity test and has the potential to improve antithrombin diagnostics and aid in personalized medicine.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Medical Laboratory Technology
Tirsa T. van Duijl, Esther N. M. de Rooij, Maxim M. Treep, Marte E. Koelemaij, Fred P. H. T. M. Romijn, Ellen K. Hoogeveen, L. Renee Ruhaak, Saskia le Cessie, Johan W. de Fijter, Christa M. Cobbaert
Summary: This study explored the potential of emerging and conventional urinary kidney injury biomarkers in different populations using LC-MS/MS analysis. The results showed that kidney injury biomarkers were increased in kidney transplant recipients with ischemia-reperfusion injury. NGAL and B2M showed the strongest response.
CLINICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Sandra L. Taylor, Robert H. Podolsky, Maria E. Montez-Rath, Emily Slade
Summary: This paper discusses the importance of ongoing professional development for collaborative biostatisticians and highlights the value of internal opportunities for skill and knowledge enhancement. It provides guidance and specific activity examples for collaborative biostatisticians and/or managers of biostatistical units to create a customized program.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Article
Medical Laboratory Technology
Tirsa T. van Duijl, L. Renee Ruhaak, Cees van Kooten, Johan W. de Fijter, Christa M. Cobbaert
Summary: This study explored the potential of LC-MS/MS as a next-generation technology for multiplex urinary protein measurement. It overcomes nonselectivity and lack of standardization by directly measuring well-defined molecular proteins.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)