期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 405, 期 14, 页码 4921-4929出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-6885-2
关键词
Gold nanoparticles-dotted graphene; CdTe quantumdots coated silica nanoparticles; Electrochemiluminescent; Immunoassay
资金
- Natural Science Research Foundation of China [21277058, 21175058, 21207048]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002]
- Technology Development Plan of Shandong Province, China [2011GGB01153]
A facile and ultrasensitive electrochemiluminescent (ECL) immunosensor for detection of prostate-specific antigen (PSA) was designed by using CdTe quantum dots coated silica nanoparticles (SiO2@QDs) as bionanolabels. To construct such an electrochemiluminescence immunosensor, gold nanoparticles-dotted graphene composites were immobilized on the working electrode, which can increase the surface area to capture a large amount of primary antibodies as well as improve the electronic transmission rate. The as-prepared SiO2@QDs used as bionanolabels, showed good ECL performance and good ability of immobilization for secondary antibodies. The approach provided a good linear response ranging from 0.005 to 10 ng mL(-1) with a low detection limit of 0.0032 ng mL(-1). Such immunosensor showed good precision, acceptable stability, and reproducibility. Satisfactory results were obtained for determination of PSA in human serum samples. Therefore, the proposed method provides a new promising platform of clinical immunoassay for other biomolecules.
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