Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Alex Gomez-Gomez, MohammadA Sabbaghi, Noemi Haro, Joan Albanell, Silvia Menendez, Monica Gonzalez, Gabriel Gil-Gomez, Ana Rovira, Oscar J. Pozo
Summary: Formalin-fixed paraffin-embedded (FFPE) tissues are crucial in cancer research, but research on the detection of small molecules in FFPE samples is limited. This study aimed to explore the potential of targeted metabolomics in FFPE specimens and developed a LC-MS/MS method for the quantification of acidic metabolites. The method showed accurate and precise quantification of 40 metabolites in FFPE samples, with implications for cancer research.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Chemistry, Organic
Shihui Luo, Chaoqun Weng, Zixuan Qin, Ke Li, Tianxiao Zhao, Yuxuan Ding, Chen Ling, Yuan Ma, Jie An
Summary: The study introduced a novel strategy for the synthesis of alpha,beta-deuterated amines, showcasing the potential application in the development of deuterated drugs. The method demonstrated high efficiency in the synthesis of bevantolol-d(4) as an example.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Fei Yang, Haozhe Cui, Chunqiong Wang, Ying Wang, Wenjing Zhu, Huimin Deng, Shanshan Liu, Zhaoyang Bian, Junli Lu, Gangling Tang, Yuan Ji
Summary: This study utilized reversed-phase liquid chromatography-tandem mass spectrometry and supercritical fluid chromatography-tandem mass spectrometry to determine the stereoisomers of chlorfenvinphos and dimethylvinphos in tobacco. A modified quick, easy, cheap, effective, rugged, and safe technique using spherical carbon was employed for the extraction and purification of tobacco samples. Both methods were compared comprehensively in terms of methods validation parameters, showing excellent linearity and adequate recoveries for the targeted stereoisomers.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Xinyue Zheng, Xujian Duan, Di Lu, Qiuhong Jiang, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin, Meiyun Shi
Summary: This study developed a highly selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method to determine butachlor in a biological matrix. The method used tandem mass spectrometry cubed scanning mode, improving selectivity and sensitivity. The results indicated that butachlor was cellular toxic.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Substance Abuse
Rachel J. Keith, Jessica L. Fetterman, Olusola A. Orimoloye, Zeina Dardari, Pawel K. Lorkiewicz, Naomi M. Hamburg, Andrew P. DeFilippis, Michael J. Blaha, Aruni Bhatnagar
NICOTINE & TOBACCO RESEARCH
(2020)
Article
Oncology
Lindsey R. Conroy, Susan Dougherty, Traci Kruer, Stephanie Metcalf, Pawel Lorkiewicz, Liqing He, Xinmin Yin, Xiang Zhang, Sengodagounder Arumugam, Lyndsay E. A. Young, Ramon C. Sun, Brian F. Clem
Article
Oncology
Lindsey R. Conroy, Pawel Lorkiewicz, Liqing He, Xinmin Yin, Xiang Zhang, Shesh N. Rai, Brian F. Clem
CANCER CELL INTERNATIONAL
(2020)
Article
Cardiac & Cardiovascular Systems
Affan B. Irfan, Claudia Arab, Andrew P. DeFilippis, Pawel Lorkiewicz, Rachel J. Keith, Zhengzhi Xie, Aruni Bhatnagar, Alex P. Carll
Summary: Smoking is associated with accelerated atrioventricular conduction, which may be mediated by an increase in dopamine production. Dopamine is inversely associated with PR interval and PR segment.
CARDIOVASCULAR TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jordan Lynch, Lexiao Jin, Andre Richardson, Ganapathy Jagatheesan, Pawel Lorkiewicz, Zhengzhi Xie, Whitney S. Theis, Gregg Shirk, Marina Malovichko, Aruni Bhatnagar, Sanjay Srivastava, Daniel J. Conklin
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2020)
Article
Medicine, General & Internal
Timothy E. O'Toole, Alok A. Amraotkar, Andrew P. DeFilippis, Shesh N. Rai, Rachel J. Keith, Shahid P. Baba, Pawel Lorkiewicz, Catherine E. Crandell, Gina L. Pariser, Christopher J. Wingard, C. Arden Pope, Aruni Bhatnagar
Article
Environmental Sciences
Katlyn E. McGraw, Daniel W. Riggs, Shesh Rai, Ana Navas-Acien, Zhengzhi Xie, Pawel Lorkiewicz, Jordan Lynch, Nagma Zafar, Sathya Krishnasamy, Kira C. Taylor, Daniel J. Conklin, Andrew P. DeFilippis, Sanjay Srivastava, Aruni Bhatnagar
Summary: Exposure to acrolein and 1,3-butadiene is associated with endothelial dysfunction and may contribute to increased risk of hypertension in individuals with elevated sympathetic tone, particularly in Black participants.
ENVIRONMENTAL RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Denise S. Tevis, Sharon R. Flores, Brandon M. Kenwood, Deepak Bhandari, Peyton Jacob, Jia Liu, Pawel K. Lorkiewicz, Daniel J. Conklin, Stephen S. Hecht, Maciej L. Goniewicz, Benjamin C. Blount, Victor R. De Jesus
Summary: The increasing interest in VOC exposure has highlighted the need for consistent naming of VOC metabolites, which currently lacks standardization. This study aims to establish a systematic acronym naming system to provide consistency in current usage and guide future acronyms for VOC metabolites. Despite the well-established measurement of VOC metabolites for investigating exposure, harmonizing acronyms among investigators has not been formally attempted.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Chemistry, Medicinal
Pawel Lorkiewicz, Rachel Keith, Jordan Lynch, Lexiao Jin, Whitney Theis, Tatiana Krivokhizhina, Daniel Riggs, Aruni Bhatnagar, Sanjay Srivastava, Daniel J. Conklin
Summary: The long-term health effects of e-cigarettes are still unknown, but studies on animal models and humans suggest that they can cause cardiovascular injury and endothelial dysfunction due to reactive aldehydes in e-cigarette aerosols. Thermal decomposition of vegetable glycerin in the e-cigarette solvent produces acrolein and glycidol, which may contribute to the adverse health effects of e-cigarettes. The urinary metabolite 23HPMA may serve as a specific biomarker for e-cigarette use.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Riham R. E. Abouleisa, Abou Bakr M. Salama, Qinghui Ou, Xian-Liang Tang, Mitesh Solanki, Yiru Guo, Yibing Nong, Lindsey McNally, Pawel K. Lorkiewicz, Kamal M. Kassem, Brooke M. Ahern, Krishna Choudhary, Reuben Thomas, Yu Huang, Hamzah R. Juhardeen, Aisha Siddique, Zainab Ifthikar, Sally K. Hammad, Ayman S. El-Baz, Kathryn N. Ivey, Daniel J. Conklin, Jonathan Satin, Bradford G. Hill, Deepak Srivastava, Roberto Bolli, Tamer M. A. Mohamed
Summary: This study provides mechanistic insights into forced cardiomyocyte proliferation and advances the clinical feasibility of this approach for the treatment of ischemic heart failure by minimizing the oncogenic potential of cell cycle factors and using a novel viral construct.
Article
Biochemistry & Molecular Biology
Riham R. E. Abouleisa, Lindsey McNally, Abou Bakr M. Salama, Sally K. Hammad, Qinghui Ou, Collin Wells, Pawel K. Lorkiewicz, Roberto Bolli, Tamer M. A. Mohamed, Bradford G. Hill
Summary: This study reveals that a metabolic shift from catabolic to anabolic activity is a critical step for cardiomyocyte cell cycle entry and proliferation, indicating the importance of metabolic changes in influencing myocyte proliferation.
Article
Cardiac & Cardiovascular Systems
Kyle L. Fulghum, Juliette B. Smith, Julia Chariker, Lauren F. Garrett, Kenneth R. Brittian, Pawel K. Lorkiewicz, Lindsey A. McNally, Shizuka Uchida, Steven P. Jones, Bradford G. Hill, Helen E. Collins
Summary: The goal of this study was to develop an atlas of the metabolic, transcriptional, and proteomic changes in the maternal heart during and after pregnancy. The results showed significant increases in heart size, cardiac hypertrophy, and myocyte cross-sectional area during pregnancy and post-birth, accompanied by changes in metabolites, gene expression, and protein levels. These findings provide important insights into the coordinated molecular changes that occur in the maternal heart during and after pregnancy.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Letter
Cardiac & Cardiovascular Systems
Andrew A. Gibb, Emma K. Murray, Anh T. Huynh, Ryan B. Gaspar, Tori L. Ploesch, Ken Bedi, Alyssa A. Lombardi, Pawel K. Lorkiewicz, Rajika Roy, Zolt Arany, Daniel P. Kelly, Kenneth B. Margulies, Bradford G. Hill, John W. Elrod
Article
Toxicology
Timothy E. O'Toole, Xiaohong Li, Daniel W. Riggs, David J. Hoetker, Ray Yeager, Pawel Lorkiewicz, Shahid P. Baba, Nigel G. F. Cooper, Aruni Bhatnagar
INHALATION TOXICOLOGY
(2020)
Article
Gastroenterology & Hepatology
Hridgandh Donde, Smita Ghare, Swati Joshi-Barve, JingWen Zhang, Manicka V. Vadhanam, Leila Gobejishvili, Pawel Lorkiewicz, Sanjay Srivastava, Craig J. McClain, Shirish Barve
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2020)