Article
Chemistry, Analytical
Cemil Can Eylem, Emirhan Nemutlu, Aysegul Dogan, Vedat Acik, Selcuk Matyar, Yurdal Gezercan, Suleyman Altintas, Ali Ihsan Okten, Nursabah Elif Basci Akduman
Summary: To gain deeper insights into the molecular mechanisms in biological systems, it is necessary to comprehensively examine a wide range of molecular species. This study develops a high-throughput and robust metabolomics and lipidomics analysis method to simultaneously analyze metabolites and lipids from a single sample, by optimizing sample preparation and method parameters. The results show that the methanol:water:chloroform (3:1:3, v/v/v) mixture is the most superior extraction solvent combination. The efficiency of derivatization steps and chromatographic parameters were also tested.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Fei Xue, Bing Wang, Dong-xiao Guo, Yang Jiao, Wei-liang Cui, Xian-long Cheng, Zhi-bin Wang, Xue Yin, Shuang-cheng Ma, Yong-qiang Lin
Summary: Deer horn gelatin (DHG) is a valuable nutritional dietary supplement, but it is difficult to assess its quality and clarify the species of its raw material. This study used advanced analytical techniques to identify peptide markers specific to DHG and developed strategies for assessing its quality. The strategies can be used to evaluate the quality of deer gelatin.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Food Science & Technology
Haopeng Dai, Min He, Guilin Hu, Zhongrong Li, Abdulbaset Al-Romaima, Zhouwei Wu, Xiaocui Liu, Minghua Qiu
Summary: The study aimed to investigate the ACE inhibitory effect of bioactive peptides from green coffee beans. Through in vitro and in silico methods, it was found that two peptides have the potential to be used as antihypertensive agents.
Article
Food Science & Technology
Ana G. Abril, Monica Carrera, Karola Boehme, Jorge Barros-Velazquez, Benito Canas, Jose-Luis R. Rama, Tomas G. Villa, Pilar Calo-Mata
Summary: This study utilized LC-ESI-MS/MS MS to analyze peptides from bacteriophages infecting mastitis-causing Staphylococcus aureus, identifying a large number of specific peptides that could be useful for identifying and characterizing the bacteria causing mastitis. Additionally, a study of bacteriophage phylogeny and its relationship with the respective Staphylococcus spp. infected was conducted, revealing specific peptides present in closely related phages and highlighting the connection between bacteriophage phylogeny and the infected Staphylococcus spp.
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lili Jin, Haibo Ding, Volkan Degirmenci, Hongchuan Xin, Qifan Miao, Qiuyu Wang, Dianbao Zhang
Summary: AWRK6, a synthesized peptide based on the natural peptide dybowskin-2CDYa, was analyzed in rat plasma using a LC-MS/MS method. Pharmacokinetic studies showed AWRK6 peaked in rat plasma at 1.2 hours after intraperitoneal injection and had a terminal half-life of approximately 2.8 hours.
Review
Chemistry, Analytical
Davide Coniglio, Giovanni Ventura, Cosima D. Calvano, Ilario Losito, Tommaso R. I. Cataldi
Summary: Arsenic-containing lipids, or arsenolipids, are organic compounds commonly found in marine organisms. Mass spectrometry techniques, such as ICP-MS and LC-ESI-MS, have played a key role in characterizing these lipids. Negative polarity and tandem MS analyses have further improved the identification of specific ions and the determination of the regiochemistry of these compounds.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Food Science & Technology
Gustavo A. Figueroa Campos, Johannes G. K. T. Kruizenga, Sorel Tchewonpi Sagu, Steffen Schwarz, Thomas Homann, Andreas Taubert, Harshadrai M. Rawel
Summary: This study investigated the changes in the protein fraction of coffee, which is important for cup quality. The results showed a significant increase in modified peptides from wet processed green beans compared to dry ones. This study contributes to a better understanding of the influence of different processing methods on protein quality and its role in coffee cup quality and aroma.
Article
Food Science & Technology
Gbemisola J. Fadimu, Asgar Farahnaky, Harsharn Gill, Olusegun A. Olalere, Chee-Yuen Gan, Tuyen Truong
Summary: The use of natural ingredients for managing diabetes has become increasingly popular due to the adverse effects of synthetic antidiabetic medications. This study investigated the potential of hydrolysates from lupin proteins treated with ultrasound and hydrolyzed using alcalase and flavourzyme to inhibit alpha-glucosidase and alpha-amylase, enzymes involved in diabetes management. Peptide sequencing and molecular docking analysis identified specific peptides with potential antidiabetic activity. These findings suggest that hydrolysates from lupin proteins could be useful in formulating functional food for managing diabetes.
Article
Food Science & Technology
Hongbo Li, Yang Zhao, Yuelu Jiang, Jiaxin Li, Jingjing Yang, Yuling Xue, Zhiyuan Kang, Justyna Zulewska, Hongjuan Li, Jinghua Yu
Summary: Milk protein concentrate (MPC) was hydrolyzed using alcalase and neutrase, resulting in reduced allergenicity. The hydrolysis conditions were determined as follows: alcalase and neutrase ratio of 1:1, enzyme to substrate ratio of 6000 U/g MPC, temperature of 50 degrees C, pH 8.5, and hydrolysis time of 3 h. The hydrolysate contained 85.31% peptides with molecular weights <1 kDa, and the antigenicity reduction rates of casein and beta-lactoglobulin were 33.76% and 22.38%, respectively. LC-MS/MS peptide identification revealed that a total of 65 epitopes of casein and 21 epitopes of whey protein were disrupted by alcalase and neutrase, providing further insight into the mechanism of reducing milk protein allergenicity through complex enzyme hydrolysis.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Cell Biology
Zhanghan Chen, Zhipeng Qi, Dongli He, Jingyi Liu, Enpan Xu, Bing Li, Shilun Cai, Di Sun, Yirong Cheng, Qiang Shi, Yunshi Zhong
Summary: This study constructed an efficient strategy for screening circRNAs with coding potential and verified its feasibility. The results showed that these circRNAs were mainly involved in promoting proliferation and invasion ability. This strategy provides a more convenient pathway for identifying circRNA-derived peptides, which may serve as potential therapeutic targets or diagnostic biomarkers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Neil A. McCracken, Sarah A. Peck Justice, Aruna B. Wijeratne, Amber L. Mosley
Summary: The study introduces a user-friendly analysis of the melt shift calculation workflow and quantitatively assesses the impact of key steps in the analysis workflow on the final output list of stabilized and destabilized proteins. It also presents a more optimized analysis workflow, illustrating the importance of selected calculation steps on the final list of reported proteins of interest in a study. The development of the R based program Inflect offers a valuable resource for the research community to analyze data from TPP and CETSA experiments.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shuddhanjali Roy, Tanushree Agarwal, Arup Das, Tanmoy Halder, Gouranga Upadhyaya, Binay Chaubey, Sudipta Ray
Summary: The C-terminal stretch in SbGPRP1 acts as an antimicrobial peptide by either binding to the bacterial membrane or targeting a specific protein on the membrane, causing membrane perturbation. The study shows the potential antimicrobial property of the C-terminal peptide against gram-positive bacteria and phytopathogen, and identifies a crucial 23 amino acid sequence for its antimicrobial activity. Further experiments demonstrate the interaction between C-SbGPRP1 and bacterial membrane protein, leading to membrane perturbation and antimicrobial activity.
PLANT MOLECULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Catherine Coremans, Cedric Delporte, Frederic Cotton, Phillipe Van de Borne, Karim Zouaoui Boudjeltia, Pierre Van Antwerpen
Summary: Oxidative modifications of HDLs and LDLs by myeloperoxidase can lead to dysfunction and increased cardiovascular disease risk, which can be quantified using liquid chromatography coupled with tandem mass spectrometry. While current standard methods for quantifying apolipoproteins based on immunoassays have limitations, mass spectrometry offers a potential and reliable alternative.
Article
Food Science & Technology
Ioana M. Barbu, Robert-Jan A. N. Lamers, Henk W. Gerritsen, Marco H. Blokland, Monique G. E. G. Bremer, Martin Alewijn
Summary: This article introduces a new method for verifying the heat-treatment status of milk in cheese by analyzing protein/peptide biomarkers to distinguish "Boerenkaas" from heat-treated milk cheese.
INTERNATIONAL DAIRY JOURNAL
(2021)
