Article
Biochemical Research Methods
Leonardo Gripp, Renato da Silva Carreira, Diana Moreira, Arthur de Lemos Scofield, Carlos German Massone
Summary: A method for determining potentially toxic substances in marine sediments was developed and successfully applied to surface sediments of Sepetiba Bay in Brazil. The method proved to be selective, sensitive, accurate, and linear, with the advantage of reducing sample handling time and consumable expenses. By using this method, it is possible to expand the dataset for similar coastal systems in developing countries with limited information on levels, risk assessment, and specific sediment quality guidelines for various organic contaminants.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Energy & Fuels
Sumit Kumar, Suryendu Dutta
Summary: The study demonstrates the improved characterisation of aromatic hydrocarbon biomarkers using comprehensive GC x GC-TOFMS, which helps in resolving many individual peaks. This technique achieved exceptional chromatographic separation of higher plant triterpenoids, triaromatic steroids, and methylated 2-methyl-2-(4,8,12-trimethyltridecyl) chromans in a complex aromatic fraction of a Cenozoic crude oil.
Article
Chemistry, Applied
Yuanyi Li, Qianqian Li, Baochun Zhang, Chunhua Shen, Yan Xu, Ke Tang
Summary: This study identified and quantified lactones in brandies from China and France using advanced techniques, revealing 17 lactones including some detected for the first time. The results showed that lactones play a role in distinguishing regional characteristics of different brandies, with some directly impacting aroma and others providing specific aroma characteristics through synergistic effects.
Article
Biochemical Research Methods
Natalia Drabinska, Aleksander Siger, Henryk Jelen
Summary: The aim of this study was to understand the formation of volatile organic compounds (VOCs) caused by thermal processing by tracking changes in the volatilome of cold-pressed oil and press cakes obtained from roasted seeds. The study found that seed roasting increased the formation of Maillard reaction products and the levels of aldehydes, ketones, and sulfur-containing compounds. It also revealed changes in non-volatile metabolites of oil and press cake. These findings provide valuable insights into rapeseed processing in cold-pressed oil production.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Masoomeh Tehranirokh, Marcel Van den Bronk, Peter Smith, Zach Dai, Kannan Ragunathan, Alina Muscalu, Simon Mills, Michael C. Breadmore, Robert A. Shellie
Summary: Liquid-liquid extraction is a widely used sample preparation technique, but faces challenges such as high organic solvent consumption and manual handling. This paper introduces a multifunction autosampler syringe with micromixer function for automated extraction with low solvent consumption. Experimental results demonstrate high recoveries for caffeine, dichloroethane, PCBs, and PAHs using this syringe, showing its potential for efficient and automated sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Feng Mao, Jie Wang, Haojie Fan
Summary: GC x GC-TOFMS was used to analyze the molecular compositions of hydrocarbons in tars produced from slow pyrolysis of a low-maturity lignite, with significantly more hydrocarbons identified in the lignite pyrolysis tar compared to the EL-tar. The study revealed that pyrolysis of lignite at 500 degrees C released a variety of biomarker hydrocarbons, including open-chain and cyclic aliphatic hydrocarbons. Additionally, GC x GC-TOFMS was able to distinguish between different isomers of terpenoid hydrocarbons and various homologues of cyclopentane/cyclopentene and cyclohexane/cyclohexene.
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
(2021)
Article
Chemistry, Applied
Xiaomeng Yuan, Junmeng Zhou, Baochun Zhang, Chunhua Shen, Lina Yu, Chuanbin Gong, Yan Xu, Ke Tang
Summary: In this study, three different extraction methods combined with comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry were employed to identify and quantify furan compounds in brandy. Liquid-liquid extraction using dichloromethane was found to be the optimal method. A total of 21 furan compounds were identified, including 5 that were detected in brandy for the first time. Quantitative results showed a positive correlation between furan compound content and aging time. Certain furan compounds with high odor activity values were found to significantly impact the sensory characteristics of brandy. These findings provide a new perspective on the sensory and chemical characteristics of brandy.
Article
Chemistry, Applied
Siliang Wu, Juan Yang, Hao Dong, Qiaoyu Liu, Xiangluan Li, Xiaofang Zeng, Weidong Bai
Summary: The key aroma compounds of dry-cured Spanish mackerel were identified using various analytical methods, with 21-26 compounds showing high flavor dilution factors identified. Results indicated that a longer dry-curing time may promote the generation of aroma compounds. Metabolism analysis suggested potential metabolic mechanisms involving the auto-oxidation/oxidation of unsaturated fatty acids and the enzymatic degradation of L-leucine.
Article
Biochemistry & Molecular Biology
Yulong Bao, Yaqi Zhang, Wanjun Xu
Summary: Freezing rate and storage temperature have a significant impact on lipid oxidation and protein denaturation in large-mouth bass fillets. Protein oxidation is more affected by freezing rate.
Article
Food Science & Technology
Yue Zhang, Yu-Hui Zhang, Han Yan, Chen-Yang Shao, Wei-Xuan Li, Hai-Peng Lv, Zhi Lin, Yin Zhu
Summary: Chiral volatiles in Wuyi rock tea were studied, and specific chemical markers as well as key aroma compounds were identified in different tea cultivars. These findings provide scientific references for tea cultivar discrimination and improvement of aroma quality in Wuyi rock tea.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Environmental Sciences
Veronica van der Schyff, Nee Sun Choong Kwet Yive, Anuschka Polder, Nik C. Cole, Vikash Tatayah, Henrik Kylin, Hindrik Bouwman
Summary: The concentrations of persistent organic pollutants (POPs) in seabird eggs from St. Brandon's Atoll showed species-specific differences, reflecting different foraging ranges and pollutant sources. The contaminant concentrations found in this study were lower than values from other studies, making St. Atoll an ideal reference site for monitoring background concentrations of POPs in the tropical Indian Ocean.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Andrea Gaion, Ellana Morgan, Stuart Collier, Davide Sartori
Summary: This research examined the concentrations of PBDE in Atlantic herring and its relationship with gender and morphometric parameters. The results showed no statistical differences between most congeners in males and females, except for one congener. The study contributes to a better understanding of the ecotoxicological properties of these molecules and their implications for human consumption.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Food Science & Technology
L. Nalbone, F. Cincotta, F. Giarratana, G. Ziino, A. Panebianco
Summary: This study investigated the presence of microplastics in three mussel species sourced from various retailers in Italy, finding a significantly higher number of microplastics in fresh mussels. The exposure to microplastics in mussels is limited in its impact on human health, but the sources of microplastics in store-bought mussels may be various and related to contamination along the food production chain. Further studies are needed to properly assess and manage the risk of microplastics in food.
Article
Chemistry, Analytical
Marco Beccaria, Marco Piparo, Yun Zou, Pierre-Hugues Stefanuto, Giorgia Purcaro, Anna Luiza Mendes Siqueira, Adrien Maniquet, Pierre Giusti, Jean-Francois Focant
Summary: This study focuses on the pyrolysis oils of plastic waste in chemical recycling, and a detailed investigation is conducted using multidimensional gas chromatography. High-resolution mass spectrometry is employed to evaluate isomeric species, and the injection method is optimized to introduce a wide range of carbon number compounds.
Article
Endocrinology & Metabolism
Axel Olander, Jean-Baptiste Raina, Caitlin A. Lawson, Natasha Bartels, Maiken Ueland, David J. Suggett
Summary: This study aims to characterize the volatilomes of key competitors in Australian temperate benthic reefs.
Article
Biochemical Research Methods
Marketa Kulisova, Olga Matatkova, Tomas Branyik, Jaroslav Zelenka, Lucie Drabova, Irena Jarosova Kolouchova
Summary: Microscopic filamentous fungi are adaptable microorganisms that can form biofilms on various surfaces. This study aimed to find a reliable method for measuring the biofilm growth of selected fungi strains. Multiple methods were tested, and crystal violet staining was found to be suitable for determining total biofilm biomass, while the MTT assay was suitable for determining its metabolic activity. Fluorescent stains successfully dyed the hyphae of microscopic fungi.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Environmental Sciences
Ondrej Parizek, Tomas Gramblicka, Denisa Parizkova, Andrea Polachova, Kamila Bechynska, Darina Dvorakova, Michal Stupak, Jiri Dusek, Jitka Pavlikova, Jan Topinka, Radim J. Sram, Jana Pulkrabova
Summary: This study conducted a biomonitoring survey on 231 human breast milk samples collected in 2019 and 2021 from two regions of the Czech Republic. The survey revealed the presence of 94 organohalogenated pollutants, including PCBs, OCPs, HFRs, PFAS, and PCNs. The concentrations of these pollutants were evaluated using advanced analytical techniques. The results showed a decline in the levels of banned POPs in Czech mothers, with higher concentrations of PCBs and OCPs observed in older breastfeeding primiparous women.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Chemistry, Analytical
Stanislava Rakusanova, Oliver Fiehn, Tomas Cajka
Summary: Current metabolomics and lipidomics studies have limitations in terms of the number of examined matrices, the breadth and scope of methods, reporting the number of metabolites, and data sharing. This article discusses the concept of metabolomics and lipidomics atlases, which characterize the quantitative distribution and relationships of metabolites in biological matrices and serve as a resource for future studies. The recommended approach includes combined sample extraction, a multiplatform mass spectrometry-based method, and the use of multiple internal standards for quantification. An interactive web tool should be developed for easy access to the generated data.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Maria Vrublevskaya, Thi Tra My Nguyenova, Lucie Drabova, Petra Lovecka, Blanka Vrchotova, Olga Matatkova, Markata Kulisova, Irena Kolouchova Jarosova
Summary: Plants have developed resistance mechanisms to cope with biotic and abiotic stress, including the presence of microbial endophytes that can promote growth and resilience. This study assessed the diversity and plant growth-promoting properties of bacterial endophytes in two vineyards with different agricultural management approaches in the Czech Republic. A total of 470 bacterial endophytes were isolated, with Bacillus being the dominant genus in both vineyards. The ability of endophytes to produce indole-3-acetic acid (IAA) and ACC deaminase varied between vineyards, with higher antioxidant activity in the conventional vineyard.
CZECH JOURNAL OF FOOD SCIENCES
(2023)
Article
Chemistry, Applied
Lucie Drabova, Jana Pulkrabova, Vojtech Hrbek, Vladimir Kocourek, Jana Hajslova
Summary: The study aimed to evaluate the concentrations of persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAHs) in 44 fish oil-based food supplements, estimate the daily intake by consumers, and assess the authenticity of the oil samples. The concentrations of SPCBs, OCPs, SPBDEs, and SPAHs found in the samples varied within certain ranges. The results also revealed that some samples labeled as fish oil were likely made from cheaper cod liver oil and had higher levels of halogenated POPs compared to supplements made from fish oil.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2023)
Article
Public, Environmental & Occupational Health
Stefanie Nuebler, Marta Esteban Lopez, Argelia Castano, Hans G. J. Mol, Johannes Mueller, Moritz Schaefer, Karin Haji-Abbas-Zarrabi, Jana Hajslova, Jana Pulkrabova, Darina Dvorakova, Katerina Urbancova, Holger M. Koch, Jean-Philippe Antignac, Amrit Kaur Sakhi, Katrin Vorkamp, Therese Burkhardt, Max Scherer, Thomas Goeen
Summary: Polycyclic aromatic hydrocarbons (PAHs) were included in the European Human Biomonitoring Initiative (HBM4EU) for harmonization and advancement of human biomonitoring in Europe. A specific QA/QC program using ICIs and EQUASs was developed to ensure comparability and accuracy of analytical laboratories. The study found that 86% of participants achieved satisfactory results in determining 13 PAH metabolites in urine, although low limits of quantification were required. The HBM4EU QA/QC program established an international network of laboratories for comparable analysis of urinary PAH biomarkers.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Water Resources
Katerina Ciglova, Veronika Vondraskova, Alena Milcova, Andrea Rossnerova, Radim J. Sram, Jan Topinka, Jana Pulkrabova
Summary: By monitoring biomarkers in urine, the study assessed personal exposure to selected chemical substances. The results showed the presence of benzophenone and camphor UV-filters, as well as metabolites of polycyclic aromatic hydrocarbons (OH-PAHs), in urine samples collected from mothers and new-borns. The concentrations of these compounds varied across regions and between different countries.
EXPOSURE AND HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Ondrej Kuda, Oliver Fiehn, Tomas Cajka
Summary: Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts was investigated over a three-day period under various temperature conditions. Results showed that storing dry extracts at -24 degrees C provided comparable results to -80 degrees C, but increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 degrees C and +30 degrees C.
Article
Chemistry, Applied
Dana Schusterova, Petr Mraz, Leos Uttl, Lucie Drabova, Vladimir Kocourek, Jana Hajslova
Summary: This study investigated pesticide residues in 49 edible mushrooms, finding 21 pesticide residues including prochloraz and carbendazim. The fate of 13 pesticides during mushroom processing was also studied, showing that most pesticide residues remained stable during processing.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2023)
Article
Cell Biology
Michaela Ferencakova, Andrea Benova, Ivan Raska Jr, Pavel Abaffy, Radek Sindelka, Martina Dzubanova, Eliska Pospisilova, Katarina Kolostova, Tomas Cajka, Ales Paclik, Vit Zikan, Michaela Tencerova
Summary: This study investigated the impact of anticoagulants on the molecular characteristics of human BMSCs during isolation and cultivation. The results showed that the type of anticoagulant did not significantly affect the molecular characteristics and cellular composition of BMSCs, while in vitro cultivation caused significant changes in the transcriptomics of BMSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Oliver Fiehn, Ondrej Kuda
Summary: Liquid chromatography-mass spectrometry (LC-MS) is crucial for analyzing complex lipids in biological samples. The impact of organic solvent quality in LC-MS analysis is often overlooked and can affect the reliability of the data. Annotation of complex lipids is prone to biases, leading to potential misidentifications and incomplete characterization.
Article
Biology
Andrea Benova, Michaela Ferencakova, Kristina Bardova, Jiri Funda, Jan Prochazka, Frantisek Spoutil, Tomas Cajka, Martina Dzubanova, Tim Balcaen, Greet Kerckhofs, Wouter Willekens, G. Harry van Lenthe, Arzuv Charyyeva, Glenda Alquicer, Alena Pecinova, Tomas Mracek, Olga Horakova, Roman Coupeau, Morten Svarer Hansen, Martin Rossmeisl, Jan Kopecky, Michaela Tencerova
Summary: This study demonstrates that supplementation of omega-3 PUFAs can improve bone health, reduce bone marrow adipose tissue formation, and promote osteoblast differentiation in obese mice.
COMMUNICATIONS BIOLOGY
(2023)
Meeting Abstract
Medicine, General & Internal
M. Dzubanova, J. M. Bond, M. Ferencakova, A. Benova, A. K. Addington, G. Kerckhofs, G. Pyka, T. Cajka, M. Kassem, C. M. Andreasen, T. L. Andersen, S. M. Craige, M. Tencerova
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Meeting Abstract
Medicine, General & Internal
O. Horakova, V. Kalendova, T. Cajka, M. Rossmeis
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Meeting Abstract
Medicine, General & Internal
A. Pecinova, P. Pecina, K. Cunatova, V. Kaplanova, Guillermo Puertas, Jan Silhavy, Marek Vrbacky, Katerina Tauchmannova, Tomas Cajka, Michal Pravenec, Josef Houstek, Tomas Acek
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)