Review
Chemistry, Analytical
Federico Stilo, Carlo Bicchi, Ana M. Jimenez-Carvelo, Luis Cuadros-Rodriguez, Stephen E. Reichenbach, Chiara Cordero
Summary: This review article summarizes the latest methods for chromatographic fingerprinting of 2D peak patterns, translating concepts of sample fingerprint and profile into comprehensive two-dimensional chromatography separations embracing the principles of biometric fingerprinting. Challenging scenarios with severe chromatographic misalignment, parallel detection, and the translation of methods from thermal to differential-flow modulated GC x GC are also considered for their relevance in specific applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Simone Squara, Friederike Manig, Thomas Henle, Michael Hellwig, Andrea Caratti, Carlo Bicchi, Stephen E. Reichenbach, Qingping Tao, Massimo Collino, Chiara Cordero
Summary: Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC x GC-TOFMS) is a powerful analytical platform for chemical investigations of complex biological samples. This study optimized a data processing strategy to compensate for 2D pattern misalignments and detector response fluctuations in saliva samples analyzed over 2 years. The strategy was trained on two batches of samples and achieved reliable peak detection and cross-alignment using a combination of untargeted and targeted pattern recognition algorithms and supervised operation with affine functions. Z-score normalization of the mass spectral total useful signal (MSTUS) was found to be the most promising strategy for cross-comparative analysis and data fusion.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Shiyuan Zhao, Haitao Zhong, Chunmei Geng, Hongjia Xue, Changshui Wang, Wenxue Sun, Ruili Dang, Wenxiu Han, Pei Jiang
Summary: In this study, gas chromatography-mass spectrometry was used to investigate the toxic mechanisms of ACR in the main target tissues of rats, revealing 14 metabolic pathways related to amino acid, fatty acid, purine, and energy metabolism. The study suggests that the toxic mechanism of ACR may involve oxidative stress, inflammation, amino acid metabolism, and energy disorders.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Changmeng Cui, Li Zhu, Qian Wang, Ruijuan Liu, Dadi Xie, Yujin Guo, Dingyi Yu, Changshui Wang, Dan Chen, Pei Jiang
Summary: The study investigated the metabolic changes after vancomycin administration in mice, revealing that vancomycin affects the metabolism in various organs, providing new insights for identification of vancomycin-induced toxicity.
Article
Medical Laboratory Technology
Yuyang Hou, Yuanping He, Jingjie Wang, Yanfen Zhou, Bo Ma, Xin Li, Liang Shi, Qi Zhang
Summary: By analyzing the metabolic profiles of four different types of asthenozoospermia, this study revealed abnormal changes in metabolites and metabolic pathways associated with the disease. The findings provide new clues and evidence for the etiology, diagnosis, and treatment of asthenozoospermia.
CLINICA CHIMICA ACTA
(2023)
Article
Agriculture, Multidisciplinary
Felipe Sanchez Bragagnolo, Gerardo Alvarez-Rivera, Marcia Cristina Breitkreitz, Elena Ibanez, Alejandro Cifuentes, Cristiano Soleo Funari
Summary: This study provides a comprehensive analysis of the metabolites in soy by-products, revealing their potential as a source of bioactive compounds that can be valorized to reduce environmental damage.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Celina Monzon, Sonia Schoneich, Robert E. Synovec
Summary: The study used GC-GC-TOFMS and PCA to investigate the effects of farming pacu fish with rice fields on the metabolome of the fish. The majority of metabolites were found to be downregulated in the farmed fish, with some involved in important metabolic processes such as protein catabolism, nucleotide metabolism, and tissue repair.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Jim Luong, Ronda Gras, Yujuan Hua, Guangyu Liu, Robert A. Shellie
Summary: This article introduces an approach using pyrolysis with comprehensive two-dimensional gas chromatography for identifying common isolated plastic polymers. The method employs a quadrupole mass spectrometer as a parallel detector to improve polymer identification in complex matrices. Various plastic polymers can be accurately identified within a total analysis time of 45 minutes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Federico Stilo, Carlo Bicchi, Stephen E. Reichenbach, Chiara Cordero
Summary: This review highlights the role of comprehensive two-dimensional gas chromatography in food-omics research, showcasing how it has introduced new concepts and tools for analyzing complex biological samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Tobias Opialla, Benjamin Gollasch, Peter H. J. L. Kuich, Lars Klug, Gabriele Rahn, Andreas Busjahn, Simone Spuler, Michael Boschmann, Jennifer A. Kirwan, Friedrich C. Luft, Stefan Kempa
Summary: The study developed a blood-drop method to assess metabolism in exercising individuals. The findings suggest that glucose availability and oxidative capacity play important roles in confronting maximal exertion.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Analytical
Weiyi Wen, Ping Xu, Haiping Xiang, Mengyi Wen, Xuemin Ye, Chu Chu, Shengqiang Tong
Summary: In this study, a comprehensive two-dimensional countercurrent chromatography (CCC) combined with gas chromatography (GC) was used to characterize the chemical constituents of Artemisia argyi essential oil and evaluate the orthogonality of the 2D chromatographic system. A solvent system composed of n-hexane/acetonitrile/methanol (2:2:1, v/v/v) was selected for the first dimensional separation and all CCC fractions were analyzed by GC. A 2D contour plot was created to visualize the chemical compositions. A total of 1392 peaks were obtained through the comprehensive 2D CCC x GC separation and high spatial coverage and low linear correlation coefficient were achieved. Moreover, all compounds were identified by GC-MS. The obtained 2D contour plot could be divided into six zones representing different component groups, which could be used to identify and predict the structure of unknown compounds in Artemisia argyi essential oil for comprehensive characterization.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Multidisciplinary
Fengke Lin, Edward J. Kennelly, Roger G. Linington, Chunlin Long
Summary: This study conducted a comprehensive metabolomic and bioactivity evaluation of different plant parts from Garcinia yunnanensis and Garcinia xanthochymus. Differences in metabolite profiles and bioactivity were observed among different plant parts. The seeds and latex of both species exhibited excellent cytotoxic and antibacterial activities, while the roots of G. xanthochymus and the arils of G. yunnanensis showed strong anti-inflammatory effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Marta Cialie Rosso, Federico Stilo, Carlo Bicchi, Melanie Charron, Ginevra Rosso, Roberto Menta, Stephen E. Reichenbach, Christoph H. Weinert, Carina I. Mack, Sabine E. Kulling, Chiara Cordero
Summary: This study focused on the detectable metabolome changes in high-quality raw hazelnuts and identified discriminant components such as amino acids, organic acids, sugars, and polyols that play important roles in distinguishing hazelnut cultivars and the impact of roasting. Roasting significantly decreased levels of certain amino acids, organic acids, and sugars in hazelnuts, leading to changes in metabolite patterns.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemical Research Methods
Marta Cialie Rosso, Federico Stilo, Simone Squara, Erica Liberto, Stefania Mai, Chiara Mele, Paolo Marzullo, Gianluca Aimaretti, Stephen E. Reichenbach, Massimo Collino, Carlo Bicchi, Chiara Cordero
Summary: This study explores the information potential of GCxGC-TOF MS combined with variable ionization energy in studying changes in saliva metabolic signatures of obese individuals. The results demonstrate the effective use of tandem ionization data and the enhanced coverage of sample fingerprints in fused data. Multivariate statistics show good patient clustering and improved explained variance with fused data, highlighting specific metabolite variations in different groups.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Nino B. L. Milani, Eric van Gilst, Bob W. J. Pirok, Peter J. Schoenmakers
Summary: Although comprehensive 2-D GC is a well-established and widely used analytical method, it is still a highly dynamic field due to numerous innovations. This review discusses recent developments in comprehensive 2-D GC technology, including exciting improvements in modulation methods, detection methods, retention modeling, and data analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Oncology
Raquel Blazquez, Eva Rietkoetter, Britta Wenske, Darius Wlochowitz, Daniela Sparrer, Elena Vollmer, Gunnar Mueller, Julia Seegerer, Xueni Sun, Katja Dettmer, Alonso Barrantes-Freer, Lena Stange, Kirsten Utpatel, Annalen Bleckmann, Hannes Treiber, Hanibal Bohnenberger, Christof Lenz, Matthias Schulz, Christian Reimelt, Christina Hackl, Marian Grade, Deram Bueyuektas, Laila Siam, Marko Balkenhol, Christine Stadelmann, Dieter Kube, Michael P. Krahn, Martin A. Proescholdt, Markus J. Riemenschneider, Matthias Evert, Peter J. Oefner, Chistoph A. Klein, Uwe K. Hanisch, Claudia Binder, Tobias Pukrop
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Oncology
Annekatrin Arlt, Frederike von Bonin, Thorsten Rehberg, Paula Perez-Rubio, Julia C. Engelmann, Katharina Limm, Sarah Reinke, Christian Dullin, Xueni Sun, Rieke Specht, Markus Maulhardt, Franziska Linke, Gertrude Bunt, Wolfram Klapper, Martina Vockerodt, Joerg Wilting, Tobias Pukrop, Katja Dettmer, Wolfram Gronwald, Peter J. Oefner, Rainer Spang, Dieter Kube
MOLECULAR ONCOLOGY
(2020)
Article
Oncology
Chidimma Agatha Akpa, Karsten Kleo, Elisabeth Oker, Nancy Tomaszewski, Clemens Messerschmidt, Cristina Lopez, Rabea Wagener, Kathrin Oehl-Huber, Katja Dettmer, Anne Schoeler, Dido Lenze, Peter J. Oefner, Dieter Beule, Reiner Siebert, David Capper, Lora Dimitrova, Michael Hummel
Article
Oncology
Anna-Luisa Luger, Nadja Lorenz, Hans Urban, Iris Dive, Anna L. Engel, Florian Strassheimer, Katja Dettmer, Pia S. Zeiner, Shabnam Shaid, Nina Struve, Malte Kriegs, Ute Hofmann, Peter J. Oefner, Patrick N. Harter, Joachim P. Steinbach, Michael W. Ronellenfitsch
Article
Biochemistry & Molecular Biology
Xueni Sun, Raffaela S. Berger, Paul Heinrich, Ibtissam Marchiq, Jacques Pouyssegur, Kathrin Renner, Peter J. Oefner, Katja Dettmer
Article
Immunology
Katie L. O'Brien, Nadine Assmann, Eimear O'Connor, Cathal Keane, Jessica Walls, Chloe Choi, Peter J. Oefner, Clair M. Gardiner, Katja Dettmer, David K. Finlay
Summary: Cellular metabolism plays a crucial role in regulating NK cell responses, with SREBP and cMyc controlling metabolic and functional responses. Polyamine synthesis is essential for NK cell glycolytic and oxidative metabolism, while spermidine supports protein translation. Inhibition of hypusination impacts NK cell metabolism and effector functions.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Julia Baier, Maximilian Gaensbauer, Claudia Giessler, Harald Arnold, Mercedes Muske, Ulrike Schleicher, Soeren Lukassen, Arif Ekici, Manfred Rauh, Christoph Daniel, Arndt Ha Rtmann, Benjamin Schmid, Philipp Tripal, Katja Dettmer, Peter J. Oefner, Raja Atreya, Stefan Wirtz, Christian Bogdan, Jochen Mattner
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Critical Care Medicine
Selena Z. Kuo, Katja Dettmer, Medini K. Annavajhala, David H. Chong, Anne-Catrin Uhlemann, Julian A. Abrams, Peter J. Oefner, Daniel E. Freedberg
Summary: The study investigated the relationship between urinary 3-IS levels and patient outcomes in the ICU, finding that low 3-IS levels were associated with fewer ICU-free days and increased one-year mortality. Further research should explore urinary 3-IS as an ICU biomarker.
JOURNAL OF CRITICAL CARE
(2021)
Article
Multidisciplinary Sciences
Jennifer K. Dowling, Remsha Afzal, Linden J. Gearing, Mariana P. Cervantes-Silva, Stephanie Annett, Gavin M. Davis, Chiara De Santi, Nadine Assmann, Katja Dettmer, Daniel J. Gough, Glenn R. Bantug, Fidinny Hamid, Frances K. Nally, Conor P. Duffy, Aoife L. Gorman, Alex M. Liddicoat, Ed C. Lavelle, Christoph Hess, Peter J. Oefner, David K. Finlay, Gavin P. Davey, Tracy Robson, Annie M. Curtis, Paul J. Hertzog, Bryan R. G. Williams, Claire E. McCoy
Summary: The study reveals that Arg2 is a protein in inflammatory macrophages regulated by miR-155 and IL-10, crucial for IL-10-induced modulation of mitochondrial dynamics and oxidative respiration. Arg2 mediates this process by increasing the activity of complex II and is essential for IL-10-mediated downregulation of inflammatory mediators.
NATURE COMMUNICATIONS
(2021)
Article
Allergy
Anders O. Petersen, Martta Jokinen, Damian R. Plichta, Gerhard Liebisch, Wolfram Gronwald, Katja Dettmer, Peter J. Oefner, Hera Vlamakis, Daniel C. Chung, Annamari Ranki, Ramnik J. Xavier
Summary: The gut microbiome in APS-1 patients is correlated with APS-1 symptoms, and probiotic intervention can alleviate some gastrointestinal symptoms. Furthermore, autoantibodies against cytokines appear to shape the gut microbiome by positively correlating with a taxonomically consistent group of bacteria.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Oncology
Raffaela S. Berger, Christian J. Wachsmuth, Magdalena C. Waldhier, Kathrin Renner-Sattler, Simone Thomas, Anuhar Chaturvedi, Hans-Helmut Niller, Elisabeth Bumes, Peter Hau, Martin Proescholdt, Wolfram Gronwald, Michael Heuser, Marina Kreutz, Peter J. Oefner, Katja Dettmer
Summary: Somatic mutations in isocitrate dehydrogenase lead to excessive production of D-2-hydroxyglutarate in certain malignancies. A related metabolite, 2-hydroxyglutarate-gamma-lactone, was discovered and it may impact the anti-tumor immune response. The formation of the lactone is likely linked to transport processes across the cell membrane and favored at low environmental pH.
Article
Biochemistry & Molecular Biology
Stephanie Arndt, Fadi Fadil, Katja Dettmer, Petra Unger, Marko Boskovic, Claudia Samol, Anja-Katrin Bosserhoff, Julia L. Zimmermann, Michael Gruber, Wolfram Gronwald, Sigrid Karrer
Summary: Cold Atmospheric Plasma (CAP) has anti-tumor effects transmitted through direct treatment or plasma-treated solutions (PTS) containing various amino acids and vitamins. Different plasma modes and treatment durations affect the production of reactive species in PTS, influencing the composition of active components and anti-tumor mechanisms. The findings are crucial for plasma device development and tumor treatment effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eva Scholler, James Marks, Virginie Marchand, Astrid Bruckmann, Christopher A. Powell, Markus Reichold, Christian Daniel Mutti, Katja Dettmer, Regina Feederle, Stefan Huttelmaier, Mark Helm, Peter Oefner, Michal Minczuk, Yuri Motorin, Markus Hafner, Gunter Meister
Summary: The mitochondrial protein METTL8 plays a crucial role in facilitating m(3)C methylation on mt-tRNAs, impacting respiratory chain activity and pancreatic cancer development. This modification leads to optimal composition and function of the mitochondria respiratory chain, with implications for patient survival and cellular metabolism.
Article
Pharmacology & Pharmacy
Philipp Tauber, Frederick Sinha, Raffaela S. Berger, Wolfram Gronwald, Katja Dettmer, Michaela Kuhn, Maximilian Trum, Lars S. Maier, Stefan Wagner, Frank Schweda
Summary: Large-scale clinical outcome studies have shown the efficacy of SGLT2 inhibitors in type II diabetes patients, with significant renoprotection observed in non-diabetic individuals with chronic kidney disease. However, the mechanisms behind the renal protective effects of SGLT2 inhibitors remain largely unknown and speculative.
FRONTIERS IN PHARMACOLOGY
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Selena Z. Kuo, Katja Dettmer, Medini J. Annavajhala, Anne-Catrin Uhlemann, Julian A. Abrams, Peter Oefner, Daniel E. Freedberg
