Review
Food Science & Technology
Isabella Supardi Parida, Soo Takasu, Kiyotaka Nakagawa
Summary: Mulberry leaves are rich in biologically active compounds, particularly mulberry leaf iminosugars (MLIs), which have been found to have various health-promoting effects. This review comprehensively summarizes existing studies on the oral pharmacokinetics and physiological significance of MLIs, suggesting their potential for therapeutic use against metabolic and chronic disorders.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Yiming Zhou, Siyuan Yu, Xuanming She, Xiaoli Zhou
Summary: Our study demonstrated that fermentation by Pediococcus pentosaceus degrades allergenic proteins in Tartary buckwheat, leading to a reduction in IgE-binding activity. Comparison with unfermented Tartary buckwheat peptides showed that 756 fragments associated with allergenic proteins were upregulated and the expression of 213 fragments was reduced by 71.83% after fermentation. Bioactivity prediction identified six peptide fragments derived from Fagt 1, potentially contributing to the residual allergenicity in the fermented product.
Article
Chemistry, Applied
Lin Han, Huiqing Wang, Junwei Cao, Yunlong Li, Xiying Jin, Caian He, Min Wang
Summary: Tartary buckwheat hull acetone extract demonstrated the best inhibition of α-glucosidase, while myricetin and quercetin were identified as the most effective inhibitors. These compounds exhibited strong inhibitory activity against α-glucosidase.
Article
Food Science & Technology
Bernat Miralles-Perez, Maria Rosa Nogues, Vanessa Sanchez-Martos, Nuria Taltavull, Lucia Mendez, Isabel Medina, Sara Ramos-Romero, Josep L. Torres, Marta Romeu
Summary: The combination of buckwheat D-fagomine (FG) and fish omega-3 polyunsaturated fatty acids (omega-3 PUFA) was found to have beneficial effects on attenuating insulin resistance induced by a high-fat diet in rats. This combination also reduced dyslipidemia, liver enzyme levels, interleukin-6, and oxidative stress. Omega-3 PUFA played a key role in modulating the beneficial effects of the combination on oxidative stress and related risk factors in pre-obese rats.
Article
Plant Sciences
Lucia Marchetti, Alessio Saviane, Antonella dalla Monta, Graziella Paglia, Federica Pellati, Stefania Benvenuti, Davide Bertelli, Silvia Cappellozza
Summary: Recent study on 1-DNJ in Morus sp.pl. leaves in Italian crops showed similar levels to Asian cultivations, indicating a potential impact of climate and genotypes on the compound's presence. The research highlights the importance of exploring agronomic practices and cultivar selection to standardize 1-DNJ levels in leaves.
Article
Biochemistry & Molecular Biology
Liliia Usoltseva, Vitaliy Ioutsi, Yuriy Panov, Mariya Antsupova, Liudmila Rozhinskaya, Galina Melnichenko, Natalia Mokrysheva
Summary: This study describes the development of an HPLC-DMS-MS/MS method for the quantitation of vitamin D compounds in serum. The method combines liquid-liquid and solid-phase extraction for sample preparation, eliminating the need for a lengthy derivatization step. Sensitivity is improved through the implementation of differential ion mobility separation. The assay shows good linearity, wide analytical range, and acceptable precision for all metabolites. The detection limit for 1,25(OH)(2)D-3 is 10 pg/mL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Andrej Zivkovic, Tomaz Polak, Blaz Cigic, Tomaz Pozrl
Summary: The study found that cold dehulled germinated buckwheat seeds have higher levels of total phenolic content and antioxidant activity, making them a promising ingredient for functional foods rich in beneficial phenolic compounds.
Article
Nutrition & Dietetics
Sara Ramos-Romero, Julia Ponomarenko, Susana Amezqueta, Merce Hereu, Bernat Miralles-Perez, Marta Romeu, Lucia Mendez, Isabel Medina, Josep Lluis Torres
Summary: This study investigated the effects of d-fagomine on gut microbiota, body weight, and inflammation markers in rats. The results showed that d-fagomine could reduce body weight gain, increase plasma acetate concentration, decrease pro-inflammatory biomarkers' concentrations, and induce changes in gut microbiota composition and diversity similar to dietary fiber.
Article
Chemistry, Organic
J. Bouquet, N. Auberger, R. Ashmus, D. King, A. Bordes, N. Fontelle, S. Nakagawa, Z. Madden, C. Proceviat, A. Kato, J. Desire, D. J. Vocadlo, Y. Bleriot
Summary: The synthesis and evaluation of seven-membered iminosugars derived from a 3S-acetamido-4R,5R,6S-trihydroxyazepane scaffold as inhibitors of functionally related exo-N-acetylhexosaminidases were reported. The study explored the effects of epimerization and C-alkylation at specific positions, leading to the identification of selective inhibitors for different hexosaminidases.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Plant Sciences
Lingrong Wen, Ting Zhou, Yueming Jiang, Liang Gong, Bao Yang
Summary: The study identified four prenylated phenolics in mulberry leaf, with moracin N demonstrating good neuroprotective activity by inhibiting oxidative stress through multiple mechanisms and enhancing antioxidant defense and regulation of iron death.
Article
Multidisciplinary Sciences
Gerard Masdeu, Luis Miguel Vazquez, Josep Lopez-Santin, Gloria Caminal, Slavko Kralj, Darko Makovec, Gregorio Alvaro, Marina Guillen
Summary: This study focused on the synthesis of a precursor of D-fagomine catalyzed by a mutant FSA immobilized on different supports, among which the glyoxal-agarose FSA derivative showed the highest efficiency and operational stability.
Article
Food Science & Technology
Yuling Wu, Zhanming Li, Hui Zhu, Run Zi, Fang Xue, Yue Yu
Summary: Tartary buckwheat has attracted more attention due to its unique chemical composition and higher efficacy in disease prevention. However, counterfeiting and adulteration of Tartary buckwheat with common buckwheat is common, leading to consumer harm and market disruption. This study used metabolomics technology to develop a new and simple identification method for Tartary buckwheat and common buckwheat, revealing differential metabolic pathways between the two. This research has important implications for quality control of related agricultural products.
Article
Biochemistry & Molecular Biology
Lucia Marchetti, Eleonora Truzzi, Ilaria Frosi, Adele Papetti, Silvia Cappellozza, Alessio Saviane, Federica Pellati, Davide Bertelli
Summary: There is a growing need for new options to treat diabetes at an early stage, and natural remedies have recently been reconsidered as potential candidates due to their low cost and effectiveness. Genus Morus plants contain active compounds with hypoglycaemic, hypolipidemic, and antioxidant effects. Current research on mulberry plants has mainly focused on Asian cultivation, where it has traditionally been used as a leaf infusion. This study characterised twelve Italian mulberry cultivars to expand our knowledge, and the results suggest a strong correlation between composition, genetics, and growing area. Leaf extracts showed inhibitory effects on carbohydrate digestive enzymes, likely due to the presence of 1-deoxynojirimycin, kaempferol, quercetin, and chlorogenic acid, acting synergistically. Additionally, the extracts exhibited antiglycative and carbonyl trapping capacities, potentially preventing long-term complications in diabetic patients related to advanced glycation end products (AGEs).
Article
Biochemistry & Molecular Biology
Soo Takasu, Isabella Supardi Parida, Yoshihiro Kojima, Toshiyuki Kimura, Kiyotaka Nakagawa
Summary: Studies have shown that converting GAL-DNJ to DNJ may enhance the therapeutic potency of mulberry leaves, leading to the proposal of an enzymatic-based method. This novel approach aims to improve the bioavailability and effectiveness of mulberry leaves through the hydrolysis of GAL-DNJ to DNJ.
Review
Biochemistry & Molecular Biology
Aparna Tripathi, MohdShamshad Ansari, Prajakta Dandekar, Ratnesh Jain
Summary: The article focuses on the analysis methods of vitamin D, particularly the measurement methods for 25-hydroxyvitamin D-2 and 25-hydroxyvitamin D-3, emphasizing the importance of standardization in clinical and research laboratories.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Sara Ramos-Romero, Asier Leniz, Daniel Martinez-Maqueda, Susana Amezqueta, Alfredo Fernandez-Quintela, Merce Hereu, Josep Luis Torres, Maria P. Portillo, Jara Perez-Jimenez
Summary: This study investigates the role of gut microbiota and microRNAs in contributing to the inter-individual variability in response to polyphenols. It suggests that differences in gut microbiota composition and miRNA expression levels may be associated with variations in metabolic response to polyphenols among individuals.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Immunology
Laia Bosch-Camos, Elisabet Lopez, Maria Jesus Navas, Sonia Pina-Pedrero, Francesc Accensi, Florencia Correa-Fiz, Chankyu Park, Montserrat Carrascal, Javier Dominguez, Maria Luisa Salas, Veljko Nikolin, Javier Collado, Fernando Rodriguez
Summary: The development of subunit vaccines against African swine fever is hindered by limited knowledge of specific ASF virus antigens and the identity of CD8(+) T-cell determinants. Various strategies were used to identify these antigens, leading to the characterization of ASFV-specific epitopes. Multiple approaches were used to identify the same ASFV determinants, reinforcing the presented results.
Article
Multidisciplinary Sciences
Francois Chauvigne, Carla Ducat, Alba Ferre, Tom Hansen, Montserrat Carrascal, Joaquin Abian, Roderick Nigel Finn, Joan Cerda
Summary: In various animal species, polyandry is common, leading to intense sperm competition, especially in fish. This study reveals that the swimming performance of teleost sperm is regulated through complex mechanisms involving peroxiporin orthologs and Ca2+ signaling. These findings indicate that teleost sperm have evolved sophisticated detoxification pathways to adapt to high osmotic stress and provide advantageous traits for postcopulatory sexual selection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Montserrat Carrascal, Aina Areny-Balaguero, Enrique de-Madaria, Karina Cardenas-Jaen, Guillermo Garcia-Rayado, Robin Rivera, Rosa Maria Martin Mateos, Isabel Pascual-Moreno, Meritxell Gironella, Joaquin Abian, Daniel Closa
Summary: This study found that the inflammatory capacity of exosomes in the early stages of acute pancreatitis strongly correlates with the final severity of the disease. The concentration of different proteins in exosomes also correlates with the clinical classification of pancreatitis. An increase in S100A8 and S100A9 in exosomes of severe pancreatitis suggests that their action is mediated by these proteins on NADPH oxidase, leading to free radical generation and inflammatory responses.
JOURNAL OF PATHOLOGY
(2022)
Article
Rheumatology
Jaxaira Maggi, Montserrat Carrascal, Lilian Soto, Oscar Neira, Maria C. Cuellar, Octavio Aravena, Eddie A. James, Joaquin Abian, Dolores Jaraquemada, Diego Catalan, Juan C. Aguillon
Summary: This study analyzed the HLA-DR-associated peptidome of synovial tissue and dendritic cells in rheumatoid arthritis patients, aiming to identify potential T-cell epitopes. The researchers discovered six new epitopes recognized by CD4 + T cells and found a correlation between the frequency of IFN-gamma-producing CD4 + T cells specific for a certain peptide and disease activity.
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Neurosciences
Marta Gonzalez-Sepulveda, Muhammad Yusof Omar, Sally Hamdon, Guofen Ma, Santi Rosell-Vilar, Noora Raivio, Doaa Abass, Anna Martinez-Rivas, Miquel Vila, Jesus Giraldo, Montserrat Carrascal, Joaquin Abian, Carles Gil, Josefa Sabria, Jordi Ortiz, David Moreno-Delgado
Summary: Synaptic events play a crucial role in the treatment of brain disorders. A study on rat brain tissue showed that dopamine spontaneously accumulated while synthesis decreased when incubated at 37 degrees C. This finding can help predict the in vivo effects of pharmacological interventions for dopamine-related disorders.
Article
Cell Biology
Pierre-Emmanuel Bonte, Yago A. Arribas, Antonela Merlotti, Montserrat Carrascal, Jiasi Vicky Zhang, Elina Zueva, Zev A. Binder, Cecile Alanio, Christel Goudot, Sebastian Amigorena
Summary: We analyzed transposable elements in glioblastoma patients and identified recurrently expressed elements that encode peptides in tumors but not in healthy tissues. These tumor-specific elements may serve as potential targets for cancer immunotherapy.
Article
Immunology
Marianne Burbage, Ares Rocarn-Arjo, Blandine Baudon, Yago A. Arribas, Antonela Merlotti, Derek C. Rookhuizen, Sandrine Heurtebise-Chretien, Mengliang Ye, Alexandre Houy, Nina Burgdorf, Guadalupe Suarez, Marine Gros, Benjamin Sadacca, Montserrat Carrascal, Andrea Garmilla, Mylene Bohec, Sylvain Baulande, Berangere Lombard, Damarys Loew, Joshua J. Waterfall, Marc-Henri Stern, Christel Goudot, Sebastian Amigorena
Summary: The noncanonical splice junctions between exons and transposable elements (TEs) have been identified as a source of tumor-specific antigens, and immunization with peptides derived from these junctions can delay tumor growth in mice. Inactivation of TE-silencing genes can result in overexpression of immunogenic junctions.
SCIENCE IMMUNOLOGY
(2023)
Article
Immunology
Antonela Merlotti, Benjamin Sadacca, Yago A. Arribas, Mercia Ngoma, Marianne Burbage, Christel Goudot, Alexandre Houy, Ares Rocafiln-Arjo, Ana Lalanne, Agathe Seguin-Givelet, Marine Lefevre, Sandrine Heurtebise-Chretien, Blandine Baudon, Giacomo Oliveira, Damarys Loew, Montserrat Carrascal, Catherine J. Wu, Olivier Lantz, Marc-Henri Stern, Nicolas Girard, Joshua J. Waterfallz, Sebastian Amigorena
Summary: Noncanonical mRNA splicing events can encode recurrent and immunogenic tumor-specific antigens in NSCLC patients.
SCIENCE IMMUNOLOGY
(2023)
Article
Food Science & Technology
Bernat Miralles-Perez, Maria Rosa Nogues, Vanessa Sanchez-Martos, Angels Fortuno-Mar, Sara Ramos-Romero, Josep L. Torres, Julia Ponomarenko, Susana Amezqueta, Xiang Zhang, Marta Romeu
Summary: The present study found that increasing consumption of inulin promoted high dominance of certain bacterial genera in feces while reducing richness and diversity. Additionally, inulin decreased cardiometabolic risk factors, eicosanoid levels in the liver, and oxidative stress in blood.
Article
Clinical Neurology
Laura Diaz-Marugan, Mattia Gallizioli, Leonardo Marquez-Kisinousky, Silvia Arboleya, Annalaura Mastrangelo, Francisca Ruiz-Jaen, Jordi Pedragosa, Climent Casals, Francisco Javier Morales, Sara Ramos-Romero, Sara Traserra, Carles Justicia, Miguel Gueimonde, Marcel Jimenez, Josep Lluis Torres, Xabier Urra, Angel Chamorro, David Sancho, Clara G. de los Reyes-Gavilan, Francesc Miro-Mur, Anna M. Planas
Summary: Severe stroke patients often experience respiratory and urinary tract infections. This is mainly due to opportunistic commensal bacteria translocating from the gut. This study investigated the mechanisms behind gut dysbiosis and poststroke infection.
Review
Nutrition & Dietetics
Karenia Lorenzo, Garoa Santocildes, Joan Ramon Torrella, Jose Magalhaes, Teresa Pages, Gines Viscor, Josep Lluis Torres, Sara Ramos-Romero
Summary: This paper reviews the bioactivities of proteins, fiber, and polysaccharides in Chlorella, as well as their role in exercise-related nutrition. It highlights the beneficial effects of Chlorella on performance, physical stamina, and fatigue reduction. It also emphasizes the importance of Chlorella as a dietary source of essential nutrients for athletes and recreational exercisers.
Article
Engineering, Environmental
Montserrat Carrascal, Ester Sanchez-Jimenez, Jie Fang, Carlos Perez-Lopez, Antoni Ginebreda, Damia Barcelo, Joaquin Abian
Summary: A large-scale proteomics approach was used to investigate the protein composition of wastewater from 10 different municipalities in Catalonia. The study revealed that excreta from humans and residues from livestock were the main sources of proteins in wastewater. These findings provide new insights into wastewater proteomics and propose specific bioindicators for wastewater-based environmental monitoring.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Francisco Moreno, Lucia Mendez, Ana Raner, Bernat Miralles-Perez, Marta Romeu, Sara Ramos-Romero, Josep Lluis Torres, Isabel Medina
Summary: A high intake of saturated fats and refined carbohydrates is associated with cognitive impairment and the aggravation of neurodegenerative diseases. This study found that this diet can lead to oxidative damage to brain proteins, but fish oil supplementation can counteract this damage.
BIOMEDICINE & PHARMACOTHERAPY
(2023)