Article
Chemistry, Multidisciplinary
Xiaoyan Liu, Yuexiao Jia, Ziwei Han, Qinghong Hou, Wei Zhang, Wenfu Zheng, Xingyu Jiang
Summary: The study developed a concentration gradient generator for diluting nanoparticles and combined it with a single-cell trapper array for evaluating cell responses accurately. The high-content system allowed real-time monitoring and precise assessment, providing a new method for large-scale screening of nanomedicines.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Dongdong Chen, Shengmin Xu, Shunlan Li, Shipin Tao, Luzhi Li, Shaopeng Chen, Lijun Wu
Summary: A novel alkane biosensor based on transcriptional factor AlkS was developed, which showed sensitive dynamic response curves for exogenous long-chain alkanes and in situ monitoring of endogenously produced alkanes. The biosensor enabled high-throughput screening of alkane-producing strains from the AAR and ADO mutant library, leading to a significant increase in alkane production.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Analytical
Yuliang Zhao, Jiazhi Lou, Hongyu Zhang, Hui Sun, Menglin Zhang, Shuyu Wang, Xiaopeng Sha, Zhikun Zhan, Ying Wang, Cuihua Ma, Wen Jung Li
Summary: In recent years, the single cell drug response (SCDR) measurement technology has gained extensive attention and become a hot topic in the fields of drug research and cell biology. Various SCDR measurement technologies with different working principles and measurement performance have emerged. This review highlights sixteen kinds of SCDR measurement technologies and provides a detailed comparison between them.
Article
Chemistry, Multidisciplinary
Melody A. Morris, Carolyn E. Mills, Justin M. Paloni, Eric A. Miller, Hadley D. Sikes, Bradley D. Olsen
Summary: Evolution shapes diverse properties of proteins. Incorporating proteins into materials is desired for applications, but high-throughput selection techniques are lacking. This study established a high-throughput platform to assess binding affinity for ordered sensing proteins. Fusion proteins library was constructed, and selected variants showed high binding in films. The assembly of fusion proteins and the functionality of binding protein are key factors in biosensing performance. Integration of directed evolution with assembled systems is necessary for designing better materials.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Multidisciplinary
Humayra Afrin, Christiancel Joseph Salazar, Mohsin Kazi, Syed Rizwan Ahamad, Majed Alharbi, Md Nurunnabi
Summary: Cardiac toxicity is a common side effect of anticancer drugs and can lead to heart dysfunction and even death. Patients with pre-existing cardiac issues are excluded from clinical trials due to the potential risk of cardiotoxicity. Early detection and monitoring of cardiac functions are necessary to prevent irreversible cardiac damage. New drug formulations and biomarker detection methods have the potential to mitigate the risk of cardiotoxicity.
CHINESE CHEMICAL LETTERS
(2022)
Article
Microbiology
Clara M. Bento, Maria Salome Gomes, Tania Silva
Summary: As infectious agents become more resistant to available drugs, the need for new and efficient treatment options grows. Conventional drug susceptibility testing methods, while reliable, require manual work and time, limiting their applicability to routine laboratory work. In vitro infection models have evolved to mimic the host environment, providing a more accurate prediction of clinical efficacy for new drugs.
Article
Biotechnology & Applied Microbiology
Tracy M. Yamawaki, Daniel R. Lu, Daniel C. Ellwanger, Dev Bhatt, Paolo Manzanillo, Vanessa Arias, Hong Zhou, Oh Kyu Yoon, Oliver Homann, Songli Wang, Chi-Ming Li
Summary: Our systematic benchmarking of seven high-throughput single-cell RNA-seq methods with 21 libraries under identical conditions revealed that the 10x Genomics 5 'v1 and 3' v3 methods exhibit higher mRNA detection sensitivity and fewer dropout events. This facilitates the identification of differentially-expressed genes and improves the concordance of single-cell profiles to immune bulk RNA-seq signatures, providing useful metrics for selecting a high-throughput single-cell RNA-seq method for profiling complex immune-cell heterogeneity.
Review
Chemistry, Analytical
Fariba Mollarasouli, Nader Zare-Shehneh, Mehrorang Ghaedi
Summary: This paper summarizes the COVID-19 pandemic, clinical features, epidemiology, and pathogenesis. It also focuses on recent advances in bioanalytical diagnostic methods for SARS-CoV-2 sensing. Additionally, it presents the mechanisms, advantages, and disadvantages of the most common biosensors for COVID-19 detection.
Article
Multidisciplinary Sciences
Alissa D. Buskermolen, Yu-Ting Lin, Laura van Smeden, Rik B. van Haaften, Junhong Yan, Khulan Sergelen, Arthur M. de Jong, Menno W. J. Prins
Summary: Various applications would benefit from the ability to continuously measure biomolecules. Here the authors describe a biosensing technology based on the free diffusion of biofunctionalized particles hovering over a sensor surface, which enables monitoring of analytes (pM-mu M) over long timespans.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Yen-Cheng Liu, Saeid Ansaryan, Xiaokang Li, Eduardo R. Arvelo, Hatice Altug
Summary: This study presents a high-throughput biosensing microarray platform for real-time monitoring of secreted proteins from living single cells. It utilizes an ultrasensitive nanoplasmonic substrate and microwell compartments to analyze secretome at single-cell resolution. The platform also allows optical imaging of cells to track morphology changes. The system demonstrated its potential by detecting interleukin-2 secretion from single lymphoma cells in real-time.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Paul Datlinger, Andre F. Rendeiro, Thorina Boenke, Martin Senekowitsch, Thomas Krausgruber, Daniele Barreca, Christoph Bock
Summary: The development of 'single-cell combinatorial fluidic indexing' (scifi) allows for cost-effective single-cell sequencing on a massive scale, combining preindexing of entire transcriptomes with single-cell RNA-seq using microfluidics. This approach significantly increases throughput and enables sample multiplexing in a straightforward manner.
Review
Chemistry, Multidisciplinary
Alon Golubchik, Luma Clarindo Lopes, Vikram Singh, Sabine Kuss
Summary: This review discusses the membrane transport of pharmaceutical molecules in bacteria, emphasizing its importance to antibiotic resistance, and highlights electrochemical and electrophysiological methods for detection and quantification. The results reveal a substantial diversity in methods and types of quantitative information collected.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Danhong Han, Jingjing Xu, Han Wang, Zhenhai Wang, Nana Yang, Fan Yang, Qundong Shen, Shengyong Xu
Summary: This study presents a stable and high-precision temperature measurement chip for monitoring the temperature fluctuations of individual cells in response to external stimuli over a normal cell life cycle. The chip utilizes temperature sensors made of Pd-Cr thin-film thermocouples, a freestanding Si3N4 platform, and a dual-temperature control system, demonstrating the feasibility of detecting local temperature fluctuations of individual cells in different proliferation cycles. Future applications may include integrating this chip with other single-cell technologies for research on cellular heat-stress response.
Article
Chemistry, Multidisciplinary
Zihan Yang, Zhihang Zhou, Tongxu Si, Zhengdong Zhou, Li Zhou, Y. Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Summary: Cancer metastasis is a major cause of cancer-related death, and it is characterized by excessive extracellular matrix deposition and increased stiffness in solid tumors. The confined migration of tumor cells in these conditions plays a crucial role in metastasis, but inhibitors specifically targeting this type of migration are rare. In this study, a microfluidic chip was designed to screen for drugs that can effectively inhibit confined migration of cancer cells. By applying this chip, three novel inhibitors of confined migration, MA-5, SB-705498, and diphenyleneiodonium chloride, were discovered in multiple cancer types. The mechanisms of action of these inhibitors were found to be targeting mitochondria, actin polymerization, and cell viability, respectively. Overall, a high-throughput microfluidic platform for screening drugs targeting confined migration was established, and three effective inhibitors were identified.
Review
Chemistry, Analytical
Maria Maddalena Calabretta, Antonia Lopreside, Laura Montali, Martina Zangheri, Luca Evangelisti, Marcello Delia, Elisa Michelini
Summary: Bioluminescence is extensively used in various bioanalytical applications, and the advancement of portable light detectors has made it possible to integrate bioluminescence detection into miniaturized devices. Different photon detectors, such as PMTs, CCDs, CMOS, SPADs, SiPMs, and smartphone-integrated CMOS, have their pros and cons, and the selection of the most suitable detector should take into account factors such as instrumental noise, power supply, imaging capability, and ease of integration.
ANALYTICA CHIMICA ACTA
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Chintan Chawda, Roisin McMorrow, Natasa Gaspar, Giorgia Zambito, Laura Mezzanotte
Summary: The use of transgenic mouse models with optical reporter genes enables in vivo imaging of cellular and molecular events, as well as accurately distinguishing immune cell types and their activities. These models are essential for studying immune cell function and evaluating the effects of immunotherapies against diseases like cancer.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Medicine, General & Internal
Chiara Donadei, Andrea Angeletti, Maria Cappuccilli, Massimiliano Conti, Diletta Conte, Fulvia Zappulo, Alessio De Giovanni, Deborah Malvi, Rita Aldini, Aldo Roda, Gaetano La Manna
Summary: This study found possible adaptive tubular mechanisms for bile acid transporters in response to cirrhosis-induced AKI through a rat model. The findings suggest that the kidneys may adjust their function in response to liver cirrhosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Marcus C. M. Stroet, Erik de Blois, Marion de Jong, Yann Seimbille, Laura Mezzanotte, Clemens W. G. M. Lowik, Kranthi M. Panth
Summary: Improving the binding properties of a contrast agent facilitated the detection of spontaneous and therapy-induced tumor necrosis in mice, potentially aiding in early determination of tumor aggressiveness and therapy efficacy. The study showed increased uptake of the improved contrast agent in doxorubicin-treated tumors, indicating therapy efficacy, and demonstrated the potential for using the agent in assessing tumor aggression and therapy effectiveness in solid tumors.
Article
Biochemistry & Molecular Biology
Bruce R. Branchini, Danielle M. Fontaine, Dawn Kohrt, Brian P. Huta, Allison R. Racela, Benjamin R. Fort, Tara L. Southworth, Aldo Roda
Summary: Researchers conducted experiments to compare the spectroscopic and luminescent properties of beetle Luc and substrates, identifying new Luc/substrate combinations with improved properties and providing live cell luminescent data relevant to in vivo imaging applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Giorgia Zambito, Gunja Mishra, Christopher Schliehe, Laura Mezzanotte
Summary: Melanoma is an aggressive type of skin cancer with a poor prognosis. Early detection is crucial for effective therapy. Recent research has shown that the interaction between melanoma cells and the immune system can aid in the development of efficient treatment strategies. This study used engineered macrophages as cell-based sensors to detect metastatic melanoma, demonstrating the potential of using optical imaging technologies to track circulating macrophages for non-invasive detection of the disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Oncology
Marica Cariello, Roberta Zerlotin, Emanuela Pasculli, Elena Piccinin, Claudia Peres, Emanuele Porru, Aldo Roda, Raffaella Maria Gadaleta, Antonio Moschetta
Summary: The activation of intestinal FXR can prevent both inflammation- and genetically-driven colorectal tumorigenesis by modulating the size and composition of bile acid pool, providing new possibilities for the therapeutic management of intestinal inflammation and tumorigenesis.
Article
Radiology, Nuclear Medicine & Medical Imaging
Adriana A. S. Tavares, Laura Mezzanotte, Wendy McDougald, Monique R. Bernsen, Christian Vanhove, Markus Aswendt, Giovanna D. Ielacqua, Felix Gremse, Carmel M. Moran, Geoff Warnock, Claudia Kuntner, Marc C. Huisman
Summary: This study surveyed and summarized the current status of preclinical imaging standardization through community survey and forum discussions. The results showed that there are still challenges in standardizing preclinical imaging techniques, with significant variations in data acquisition, processing, and reporting, and limited awareness of international guidelines for preclinical (imaging) research practices.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Riccardo Desiderio, Elisa Michelini
Summary: Biogenic amines (BAs), nitrogenous molecules present in various foods, can indicate freshness and food quality as their levels increase during spoilage. Detecting BAs in real time using smart packaging is crucial for ensuring food safety and meeting consumer demands. This study developed a user-friendly colorimetric sensing paper that can visually detect BAs. The paper sensor, using the natural cross-linking agent genipin, was able to produce water-soluble blue pigments when binding with BAs. The sensor was successfully applied to monitoring chicken meat quality and achieved a limit of detection equivalent to 0.1 mM of putrescine through quantitative analysis using a smartphone camera. The sensor's suitability was verified by integrating it into smart packaging and analyzing commercial chicken meat samples stored at different temperatures, with results aligning with the labeled best before date and confirming the sensor's potential as a smart label.
Article
Chemistry, Analytical
Hector Martinez-Perez-Cejuela, Maria Maddalena Calabretta, Valerio Bocci, Marcello D'Elia, Elisa Michelini
Summary: ATP determination is commonly used for microbial contamination assessment in different settings. However, commercially available kits require controlled temperature storage and transportation, limiting their applicability. To address this issue, a new bioluminescence paper biosensor was developed by combining a new luciferase mutant with a metal-organic framework (MOF). This sensor showed improved bioluminescence signal and stability, and successfully analyzed ATP content in complex biological matrices with low detection limits. The developed sensor has potential applications in various fields requiring ATP monitoring.
Review
Chemistry, Analytical
Denise Gregucci, Faisal Nazir, Maria Maddalena Calabretta, Elisa Michelini
Summary: The United Nations Agenda 2030 Sustainable Development Goal 6 aims at ensuring the availability and sustainable management of water and sanitation. Conventional methods of water contaminant monitoring are labor-intensive, time-consuming, and costly, lacking information about water toxicity. Bioluminescence-based sensing platforms offer innovative and promising solutions for water management, ecosystem protection, and community well-being.
Article
Immunology
Roisin McMorrow, Giorgia Zambito, Alex Nigg, Karishma Lila, Thierry P. P. van den Bosch, Clemens W. G. M. Lowik, Laura Mezzanotte
Summary: In this study, a dual color bioluminescent mouse model was used to investigate the location and function of T-cells during tumor growth and checkpoint blockade treatment. The results showed differences in total T-cell bioluminescence between highly infiltrated 'hot' tumors and poorly infiltrated 'cold' tumors. An increase in bioluminescence signal was observed in the tumor-draining lymph nodes after checkpoint blockade treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rossana Comito, Emanuele Porru, Nicolo Interino, Matteo Conti, Rossella Terragni, Roberto Gotti, Marco Candela, Patrizia Simoni, Aldo Roda, Jessica Fiori
Summary: Bile acids play a key role in the gut-liver axis, and the study found that faecal oxo-metabolites from oxidation/epimerization reactions are associated with the occurrence of chronic inflammatory enteropathy.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Elisa Michelini
Summary: Bioluminescence, the emission of light in living organisms, is an important tool for various applications such as bioassays and biosensors. Recent advancements in the development of synthetic luciferases have led to improved properties for in vitro and in vivo biosensing. In this study, two new synthetic luciferases were designed and characterized, and their potential applications were demonstrated through an inflammation assay with promising results.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Elisa Michelini
Summary: Bioluminescence has become an essential tool in various applications such as bioassays, biosensors, and in vivo imaging. This study focuses on the development of new luciferases with optimized properties, including improved thermostability, pH-insensitive emission, high quantum yield, extended kinetics, and red-shifted emission. Two new synthetic luciferases were designed and characterized, showing promising features as reporter proteins and bioluminescent probes. As a proof-of-principle application, an inflammation assay using 3D cell cultures was developed, demonstrating the analytical performance of the luciferases.