Article
Chemistry, Multidisciplinary
Anil Chandra, Saumya Prasad, Helena Iuele, Francesco Colella, Riccardo Rizzo, Eliana D'Amone, Giuseppe Gigli, Loretta L. del Mercato
Summary: This study presents a methodology for noncovalent HPTS immobilization on silica microparticles, considering the retention of pH sensitivity and long-term stability. The importance of surface charge in governing the sensitivity of immobilized HPTS dye molecules on silica microparticles is emphasized in the study. The immobilization methodology's role in preserving sensitivity and stability of the microsensors is also assessed.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Editorial Material
Chemistry, Multidisciplinary
Anil Chandra, Saumya Prasad, Helena Iuele, Francesco Colella, Riccardo Rizzo, Eliana D'Amone, Giuseppe Gigli, Loretta L. del Mercato
Summary: This study presents a methodology for noncovalent HPTS immobilization on silica microparticles that takes into account the retention of pH sensitivity and the long-term stability of pH microsensors. The importance of surface charge in governing the sensitivity of the immobilized HPTS dye molecules on silica microparticles is emphasized, as well as assessing the immobilization methodology's preservation of sensitivity and stability of the microsensors.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Anil Chandra, Saumya Prasad, Helena Iuele, Francesco Colella, Riccardo Rizzo, Eliana D'Amone, Giuseppe Gigli, Loretta L. del Mercato
Summary: The optical fluorescent pH sensors based on positively charged silica microparticles containing anionic pyranine molecules were developed using a modified Stober method, ensuring overly pH-sensitive nature of pyranine and long-term stability through silica shell coating. These cytocompatible ratiometric pH sensors can be individually observed and are suitable for pH sensing and tracking applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Applied
Yanlan Huang, Ziyi Zhang, Lili Ma, Hua Zhang, Jinwu Yan, Jingjing Wu, Chuanxiang Liu
Summary: pH imaging and measurement using fluorescent probes in biological systems is highly active and urgently demanded. A new ratiometric near-infrared fluorescent probe DCIQ based on conjugated dicyanoisophorone and quinoline was obtained for pH sensing. The DCIQ probe demonstrated advantages in terms of near-infrared emission signal, large Stockes shift, high selectivity, and good sensitivity.
Article
Biochemistry & Molecular Biology
Angelo Scarciglia, Enza Di Gregorio, Silvio Aime, Giuseppe Ferrauto
Summary: In this study, the fluorescence changes of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) under different conditions were investigated. The effects of mono-, di-, and trivalent cations on the fluorescence were analyzed, and a method for the quantification of trivalent cations was proposed. The study demonstrated the feasibility of a qualitative method to investigate the intracellular release of Gd3+ upon the administration of Gd-based contrast agents in murine macrophages.
Article
Spectroscopy
Tianran Wang, Qidan Pang, Zhipu Tong, Minna Wang, Nao Xiao
Summary: A newly designed Schiff base probe JN has been synthesized for highly selective and sensitive detection of Al3+ and PPi ions in aqueous medium. The sensing mechanism of JN towards Al3+ and the detection ability of JN-Al3+ complex towards PPi ions were determined through spectra studies and theory calculations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Ondrej Podrazky, Jan Mrazek, Jana Probostova, Sona Vytykacova, Ivan Kasik, Sarka Pitrova, Ali A. Jasim
Summary: The study demonstrates the practical application of pH measurement in real biological samples using an in-house developed fiber-optic pH sensor system. A specific fluorescent dye and laser diodes were utilized for pH measurement in aqueous humor samples from patients undergoing different types of cataract surgery, showing varying pH values.
Article
Spectroscopy
Chang Liu, Huimin Yan, Jianhong Wu, Zhiming Wang, Song He, Liancheng Zhao, Xianshun Zeng
Summary: A new near-infrared fluorescent probe Qcy-OH based on an acceptor-donor-acceptor (ADA) pi-electron system with a turn-on mechanism was developed for monitoring pH fluctuations in biological systems. The probe showed a strong pH-sensitive response and high selectivity for intracellular lysosomes, enabling the tracking of lysosomal pH changes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Ching-Hsuan Tung, Myung Shin Han, Zhenhua Shen, Brian D. Gray, Koon Y. Pak, Jianguang Wang
Summary: CypH-11 is a pH-responsive fluorogenic dye designed for highlighting cancerous tissues during surgery, with fast absorption and bright fluorescence in acidic environments. Its spray-and-see concept enables real-time identification of small cancerous lesions for optimal resection without systemic toxicity.
Article
Chemistry, Multidisciplinary
Jie Yuan, Rong Peng, Dan Cheng, Liang-Hua Zou, Lin Yuan
Summary: A new mitochondria-targeted probe NIR-OH-1 was synthesized for sensitive detection of cellular mitochondrial pH changes, showing excellent photo-stability and reversibility. It demonstrated the potential for accurate real-time monitoring of pH-related pathological events in living cells under different stimuli.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Dinesh Maity, Sibshankar Bari, Pritam Ghosh, Partha Roy
Summary: The new Schiff-base molecule H4LCl serves as a colorimetric and fluorescent pH probe, with fluorescence intensity and quantum yield increasing as pH of the medium increases. The pKa value is 7.18, with intensity enhancement occurring via an ICT-AIE controlled pathway, and naked eye detection is possible by noting color changes.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Sha Wang, Zhongyan Zhang, Zijie Huang, Xiaolan Lei, Yan Wang, Ligui Li, Liting Yang, Hong Liu, Fengqiang Sun, Li-Jun Ma
Summary: A pH fluorescence probe based on pyrene with dual-pKa values was developed, showing high sensitivity and ability to generate pyrene excimers under acidic conditions. The fluorescence response of MPCA is quenched in acidic environments and enhanced under neutral conditions.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xingzong Wang, Lei Wang, Tengda Jin, Keju Sun, Jingyue Yang
Summary: Two dual-mode fluorescent probes responding to both pH and viscosity were designed and synthesized in this study based on inhibited intramolecular charge transfer (ICT) and twisted intramolecular charge transfer (TICT) processes. The fluorescence intensity of the probes would be doubly amplified at low pH and high viscosity, providing excellent contrast for biological imaging. Fluorescence imaging in living cells, tissue, and organs further demonstrated the selective visualization of cancer by the synthesized probes.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Spectroscopy
Yongshan Ma, Yanzhao Xia, Xiaodi Wang, Guangxiang Ma, Fengxia Zhang, Tianyi Jiang, Yanyan Zhu, Xuemei Li
Summary: Two novel fluorescent probes (P1 and P2) containing a perylene tetra-(alkoxycarbonyl) derivative (PTAC) and its copper chelate were designed and synthesized. The probes showed high selectivity and sensitivity towards fluoride ions, with their sensing mechanism involving the formation of H-bond between the O-H moiety and fluoride ions. The coordination of copper ion enhanced the H-bond donor capacity of the receptor unit (O-H).
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Puneet Jain, Takuya Aida, Masahiro Motosuke
Summary: Fluorescence anisotropy is utilized as a temperature sensor to measure temperature fields in microfluidics in this study. The relationship between molecule size and viscosity is crucial in obtaining a large temperature coefficient in anisotropy. Additionally, the potential of temperature imaging is confirmed through the measurement of temperature distribution under focused laser heating.
Article
Engineering, Chemical
Jan Mrazek, Sandip Bysakh, Roman Skala, Ales Mracek, Anirban Dhar, Ivo Barton, Ivan Kasik
Summary: A versatile sol-gel approach is presented for the synthesis of nanocrystalline (Eu0.5Y0.5)(2)Ti2O7. The crystallization kinetics, nucleation mechanism, and crystal structure were studied. The results showed a regular distribution of Eu3+ and Y3+ ions and provided key information for tailoring the structural properties of nanocrystalline powders for photonic applications.
ADVANCED POWDER TECHNOLOGY
(2022)
Article
Optics
Petr Varak, Ivan Kasik, Pavel Peterka, Jan Aubrecht, Jan Mrazek, Michal Kamradek, Ondrej Podrazky, Ivo Barto, Marcin Franczyk, Ryszard Buczynski, Pavel Honzatko
Summary: This study investigates the influence of various optical fiber fabrication processes on the fluorescence decay of commonly used RE ions in fiber lasers and amplifiers. It is found that heat treatment and fiber drawing accelerate the fluorescence decay, while preform elongation has a smaller effect. Overall, the heat treatment of RE-doped silica glass preforms leads to a decline in fluorescence decay.
Article
Engineering, Electrical & Electronic
Marcin Franczyk, Dariusz Pysz, Ryszard Stepien, Jaroslaw Cimek, Rafal Kasztelanic, Fang Lin Chen, Mariusz Klimczak, Luming Zhao, Ivan Kasik, Pavel Peterka, Ryszard Buczynski
Summary: We present the experimental verification of a new type of active nanostructured fiber that can emit laser at two wavelengths simultaneously. The fiber core is made of nanorods doped with Yb3+ or co-doped with Yb3+/Er3+ ions, allowing control of relative gain and modal properties. The double-cladding fiber structure enables efficient excitation with a single pump laser, and a high-quality beam is generated. The laser operation was successfully demonstrated at output power levels of 0.78 W and 0.42 W at 1040 nm and 1534 nm, respectively.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Physics, Multidisciplinary
Dennis C. Kirsch, Anastasia Bednyakova, Petr Varak, Pavel Honzatko, Benoit Cadier, Thierry Robin, Andrei Fotiadi, Pavel Peterka, Maria Chernysheva
Summary: By utilizing Thulium-doped fibre with multiple functionalities in the laser cavity, we have achieved self-driven mode-locking and broadband wavelength tuneability, demonstrating a highly stable sub-picosecond pulse generator.
COMMUNICATIONS PHYSICS
(2022)
Article
Materials Science, Ceramics
V. Puchy, P. Hvizdos, M. Hrubovcakova, L. Falat, J. Mrazek, M. Vojtko, O. Milkovic, M. Podobova
Summary: The study focuses on preparing silicon carbide (SiC) ceramic material using spark plasma sintering (SPS) technique without any additives. The effects of fiber laser ablation on the SiC surface and subsequent incorporation of graphene nanoplatelets (GNPs) into the microcraters were investigated in terms of their influence on the tribological behavior of SiC-graphene composite surfaces. The results showed that laser treatment created suitable surface morphology for GNPs incorporation, and the incorporation reduced abrasive wear on the composite surfaces.
CERAMICS INTERNATIONAL
(2023)
Article
Optics
Bara Jirickova, Martin Grabner, Cesar Jauregui, Jan Aubrecht, Ondrej Schreiber, Pavel Peterka
Summary: Investigating the temperature dependence of spectroscopic parameters of trivalent thulium ions is crucial for the development of high-power, thulium-doped fiber lasers and amplifiers. In this study, the thulium absorption/emission cross sections were determined in the temperature range of -196℃ to 300℃ and the spectral range of 700-2200 nm using a thulium-doped fiber. Attempts were made to estimate the temperature dependence of the spectra through fitting to a multi-Gaussian model and analyzing a physically motivated model of the cross section spectra.
Article
Materials Science, Ceramics
Jan Mrazek, Sona Kamradkova, Jiri Bursik, Roman Skala, Ivo Barton, Petr Varak, Yauhen Baravets, Ondrej Podrazky
Summary: We propose a sol-gel approach to produce low-phonon nanocrystalline (HoxY1-x)(2)Ti2O7, x = <0.01, 0.40>, which exhibits luminescence in the spectral range of 2000-3000 nm. The nanocrystalline structure and the impact of composition and phonon energy on luminescence properties were investigated. Ho3+ ions were found to be distributed regularly within the pyrochlore crystal lattice, resulting in an increase in the unit cell parameter. The maximum luminescence intensity at 2025 nm was observed for the composition (Ho0.03Y0.96)(2)Ti2O7. With an increasing content of Ho3+ ions, the radiative lifetime at 2025 nm regularly decreased from 6.32 to 0.22 ms. The samples had a phonon energy smaller than 700 cm(-1), allowing for an extended luminescence spectral range up to 2900 nm. Further optimization of the chemical composition can enhance the emission at 2860 nm, making it a promising thermally and chemically stable alternative to conventional fluoride or chalcogenide glasses.
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Marie Pospisilova, Hana Kalabova, Gabriela Kuncova
Summary: In this paper, we analysed the fluorescence spectra of cell suspensions containing healthy and carcinoma fibroblast mouse cells using a genetic-algorithm-spectra-decomposition software. Compared to other algorithms, this software considers the effect of light scatter. Light scatter in cell suspensions depends on the number of cells, their size, shape, and coagulation.
Proceedings Paper
Engineering, Electrical & Electronic
Jan Pokorny, Jan Aubrecht, Pavel Peterka
Summary: We present preliminary characterization of an optical fiber amplifier for wavelengths beyond the L-band based on a core-pumped thulium-doped fiber developed and fabricated in-house. We explore the adaptation of an existing broadband fiber-optic thulium-doped source for signal amplification. With the existing setups, amplification of >30 dB at 1950 nm wavelength is achievable with a maximum output power of approximately 300 mW.
MICRO-STRUCTURED AND SPECIALTY OPTICAL FIBRES VII
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Petr Varak, Ivan Kasik, Pavel Peterka, Jan Mrazek, Michal Kamradek, Jan Aubrecht, Ondrej Podrazky, Ivo Barton, Marcin Franczyk, Ryszard Buczynski, Pavel Honzatko
Summary: The fluorescence lifetime of holmium-doped aluminosilicate fibers is an important parameter for evaluating their suitability in fiber lasers. The diffusion of dopants and changes in the ion environment were identified as the main reasons for the decrease in fluorescence lifetime during the fabrication process. The fluorescence lifetime decreased gradually with the application of different fabrication processes.
MICRO-STRUCTURED AND SPECIALTY OPTICAL FIBRES VII
(2022)
Proceedings Paper
Optics
Petr Varak, Ivan Kasik, Pavel Peterka, Jan Mrazek, Michal Kamradek, Jan Aubrecht, Ondrej Podrazky, Ivo Barton, Marcin Franczyk, Ryszard Buczynski, Pavel Honzatko
Summary: This paper investigates the influence of various nanostructured-core fiber fabrication processes on the fluorescence lifetime of Yb3+ ions. The study finds that the time-resolved photoluminescence properties of Yb3+ ions in silica glass strongly depend on the processing of the material, with fiber drawing exhibiting a stronger effect compared to preform elongation.
FIBER LASERS XIX: TECHNOLOGY AND SYSTEMS
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
P. Varak, J. Mrazek, W. Blanc, J. Aubrecht, M. Kamradek, O. Podrazky, P. Honzatko
Summary: Phase-separated fibers of SiO2-ZrO2 system doped with thulium and holmium ions were prepared using a modified solution-doping method combined with MCVD. The fibers showed strong emission in the near-infrared region and exhibited double exponential photoluminescence decay. The thulium-doped fiber demonstrated laser operation threshold and slope efficiency, while the holmium-doped fiber did not show lasing properties. Improved laser performance may be achieved by incorporating a higher proportion of rare-earth ions in the favorable environment of nanoparticles.
MICRO-STRUCTURED AND SPECIALTY OPTICAL FIBRES VII
(2021)
