Review
Food Science & Technology
Bo Wang, Hang Wang, Xubin Lu, Xiangfeng Zheng, Zhenquan Yang
Summary: Foodborne pathogens have significant effects on human health and the economy. Various foodborne pathogens, including Salmonella spp., Escherichia coli, Staphylococcus aureus, Shigella spp., Campylobacter spp., and Listeria monocytogenes, are found in agricultural products, dairy products, animal-derived foods, and the environment. These pathogens in different types of food and water can cause life-threatening diseases and develop resistance to antibiotics. Traditional detection methods are time-consuming, labor-intensive, and expensive, necessitating the urgent need for fast detection methods. Electrochemical biosensors provide accurate and reproducible results for the quick detection of foodborne pathogens in food and the environment. This paper provides a comprehensive overview of electrochemical biosensors for the detection of foodborne pathogens, discusses risk analysis methods and control measures, and highlights recent research progress and future possibilities.
Review
Food Science & Technology
Yunhao Lu, Hao Yang, Jinrong Bai, Qiang He, Ruijie Deng
Summary: Foodborne pathogenic infections have caused various problems in human life, and there is a need for advanced detection technologies. CRISPR/Cas-based biosensors have the potential to address the challenges of conventional assays such as insensitivity, long turnaround time, and complex pretreatments. This review focuses on the strategies of CRISPR/Cas-assisted diagnostics for foodborne pathogens, specifically discussing biosensing platforms based on fluorescence, colorimetric, (electro)chemiluminescence, electrochemical, and surface-enhanced Raman scattering detection. The review summarizes the detection principles for foodborne pathogenic bacteria, fungi, and viruses. The current challenges and technical barriers of these methods in broad application are also discussed, along with alternative solutions to improve the potential of CRISPR/Cas for food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Review
Chemistry, Analytical
Manickam Ramesh, Ravichandran Janani, Chinnaiyan Deepa, Lakshminarasimhan Rajeshkumar
Summary: Biosensors are modern engineering tools widely used for various applications,including industrial process control, environmental monitoring, and healthcare. Nanotechnology integration enhances their performance and sensing ability. Nanomaterials like nanoparticles and nanowires are commonly used in manufacturing nano-biosensors.
Review
Chemistry, Multidisciplinary
Nguyen Nhat Nam, Hoang Dang Khoa Do, Kieu The Loan Trinh, Nae Yoon Lee
Summary: Nanotechnology provides solutions for detecting chemical and biological contaminations in the food supply chain by improving analytical performance and enhancing traditional detection techniques.
Article
Food Science & Technology
Han Du, Xiaoman Wang, Qingli Yang, Wei Wu
Summary: Quantum dots show great potential in detecting foodborne pathogens, providing rapid and sensitive monitoring of target bacteria. Compared to other methods, quantum dots are more cost-effective, and detection strategies associated with smartphones help achieve new breakthroughs.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiuli Dong, Ping Wang, Jasmine P. Darby, Yongan Tang, Christopher M. Overton, Sophia Kathariou, Ya-Ping Sun, Liju Yang
Summary: Foodborne pathogens have long been a major challenge for the food industry, and there is a critical need for new tools to control and prevent contaminations. This study demonstrated the effectiveness of carbon dots in photoinactivating Listeria monocytogenes and Salmonella cells, suggesting them as promising agents for foodborne pathogens. The study contributes to the practical application of CDots as new nanomaterial-based photodynamic inactivation agents.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Mahdi Ghorbani, Rahim Molaei, Mehran Moradi, Hossein Tajik, Fatemeh Salimi, Seyedeh Alaleh Kousheh, Mahmoud Esmaeili Koutamehr
Summary: This study investigated the reduction/degradation capability of carbon dots on biogenic amines produced by Enterococcus faecalis and Escherichia coli. The optimal concentrations of CDs and incubation time for reducing BAs were determined using response surface methodology and central composite design. CDs showed high degradation rates for certain BAs, with concentration of CDs being the major influencing factor on BAs degradation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Xiaoying Han, Yuanhui Liu, Juxin Yin, Min Yue, Ying Mu
Summary: The global burden of foodborne diseases is significant, with foodborne pathogens being the major cause of human illnesses. Microfluidic technology enables rapid, cost-effective, sensitive, and portable pathogen detection to meet on-site testing requirements. Multiplexed detection technology offers a new opportunity to ensure food safety by simultaneously detecting multiple pathogens.
FOOD RESEARCH INTERNATIONAL
(2021)
Review
Chemistry, Physical
Camelia Ungureanu, Gratiela Teodora Tihan, Roxana Gabriela Zgarian, Irina Fierascu, Anda Maria Baroi, Silviu Raileanu, Radu Claudiu Fierascu
Summary: Nowadays, the importance of quality control and food safety is emphasized. Issues with food quality not only affect the industry, but also consumers and society, leading to economic losses, changes in product quality, and posing health risks to consumers. Therefore, the development of rapid analytical systems for assessing the sanitary quality of food products, particularly the detection of pathogenic microorganisms, is crucial. Utilizing efficient and environmentally friendly detection systems to identify and convert pathogens into food waste can enhance agri-food quality throughout the entire food chain. This review paper explores the use of metal nanoparticles, especially those synthesized through green chemistry methods, as bio nanosensors in addressing these challenges. Furthermore, the potential applications of phytosynthesized nanoparticles in other biosensing areas are also discussed, highlighting the need for further exploration.
Review
Food Science & Technology
Han Du, Zhaojie Li, Yi Wang, Qingli Yang, Wei Wu
Summary: This study provides an overview of the use of nanomaterial-based optical biosensors in the detection of foodborne bacteria. It highlights the different molecular recognition elements and utilization of diverse nanomaterials in optical biosensors. The article also compares the analytical performance of nanostructured biosensors and conventional techniques, while identifying the key challenges for nanomaterial-based optical biosensors in the future.
FOOD REVIEWS INTERNATIONAL
(2022)
Review
Food Science & Technology
Emmanuel W. Bumunang, Rahat Zaheer, Dongyan Niu, Claudia Narvaez-Bravo, Trevor Alexander, Tim A. McAllister, Kim Stanford
Summary: Foodborne illness is a growing concern due to emerging pathogens, persistent contamination, antimicrobial resistance, changing environment, and complex food production systems. Common pathogens like STEC, Salmonella, Campylobacter, and Listeria monocytogenes are increasingly prevalent. Bacteriophages show promise in improving food safety and public health, but their effectiveness is limited compared to antibiotics. This review explores the applications of phages in food production systems and discusses strategies to enhance their utility for better food safety.
Article
Electrochemistry
Veronika Subjakova, Veronika Oravczova, Marek Tatarko, Tibor Hianik
Summary: Bacterial pathogens, a major contaminant in food and drinking water, require fast and sensitive detection methods. While conventional detection methods are laborious, biosensors, especially immuno- and aptamer-based electrochemical sensors, offer a promising alternative. The review analyzes current trends in developing immuno- and aptasensors for various foodborne pathogens, presenting sensitivity, regeneration capability, and application potential in food and water samples.
ELECTROCHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Kristyna Pavelicova, Lucie Vanickova, Yazan Haddad, Lukas Nejdl, Jan Zitka, Silvia Kociova, Filip Mravec, Tomas Vaculovic, Mirek Macka, Marketa Vaculovicova, Vojtech Adam
Summary: A new method for characterizing protein oligomerization based on FRET and capillary electrophoresis was reported, utilizing QDs and Cy3 as the donor-acceptor pair to study metallothionein dimer formation in the presence of Cd2+ ions. The successful monitoring and confirmation of MT dimer formation were achieved under aerobic conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Nuo Duan, Kexin Ren, Mingqian Song, Xinyue Zhang, Zhouping Wang, Fei Jia, Shijia Wu
Summary: In this study, a dual-donor fluorescence resonance energy transfer (FRET) aptasensor was developed for simultaneous detection of histamine (HIS) and tyramine (TYR) using quantum dots and molybdenum sulfide nanosheets. The method showed good sensitivity and detection limits, and can be applied to the sensitive detection of biogenic amines in animal-derived foodstuff.
MICROCHEMICAL JOURNAL
(2023)
Article
Food Science & Technology
Cuiyun Zhang, Lunjie Huang, Hongbin Pu, Da-Wen Sun
Summary: MagSERS platforms, combining magnetic properties and SERS spectra, have shown promising prospects in simplifying, speeding up, and enhancing the sensitivity of biosensors for foodborne biohazards detection. The integration of magnetic capture, separation, and enrichment with SERS substrates, as well as magnetism-mediated hotspot engineering of SERS substrates, is key to the success of MagSERS in food safety analysis.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Krishna Kant, Mohammad-Ali Shahbazi, Vivek Priy Dave, Tien Anh Ngo, Vinayaka Aaydha Chidambara, Linh Quyen Than, Dang Duong Bang, Anders Wolff
BIOTECHNOLOGY ADVANCES
(2018)
Article
Biophysics
Aaydha C. Vinayaka, Tien A. Ngo, Krishna Kant, Pia Engelsmann, Vivek P. Dave, Mohammad-Ali Shahbazi, Anders Wolff, Dang D. Bang
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Analytical
Trieu Nguyen, Aaydha Chidambara Vinayaka, Dang Duong Bang, Anders Wolff
Article
Microbiology
Than Linh Quyen, Steen Nordentoft, Aaydha Chidambara Vinayaka, Tien Anh Ngo, Pia Engelsmenn, Yi Sun, Mogens Madsen, Dang Duong Bang, Anders Wolff
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Chemistry, Analytical
Aaydha C. Vinayaka, Tien A. Ngo, Trieu Nguyen, Dang D. Bang, Anders Wolff
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Trieu Nguyen, Vinayaka Aaydha Chidambara, Sune Zoega Andreasen, Mohsen Golabi, Van Ngoc Huynh, Quyen Than Linh, Dang Duong Bang, Anders Wolff
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Food Science & Technology
Zahra Dehghani, Trieu Nguyen, Mohsen Golabi, Morteza Hosseini, Ali H. Rezayan, Javad Mohammadnejad, Anders Wolff, Aaydha C. Vinayaka
Summary: The study developed a catalytic nano-bioprobe immobilized with a Salmonella-specific DNA aptamer on magnetic beads, enabling rapid detection of low levels of Salmonella enterica serovar Typhimurium in food and chicken fecal samples; in less than 3 hours, as low as 10-15 CFU/mL of the bacteria could be captured and detected with the combination of loop mediated isothermal amplification (LAMP) technology.
Article
Biochemical Research Methods
Aaydha C. Vinayaka, Mohsen Golabi, Thi Linh Quyen Than, Anders Wolff, Dang D. Bang
Summary: Invasive non-typhoidal salmonellosis is gaining attention as an emerging disease cluster, particularly among immunocompromised and malnourished children. Point-of-care diagnostics hold promise for use in low infrastructure laboratory settings, but face challenges in dealing with the complexity of blood matrices.
Article
Immunology
Than Linh Quyen, Aaydha Chidambara Vinayaka, Mohsen Golabi, Huynh Van Ngoc, Dang Duong Bang, Anders Wolff
Summary: This study developed a new LAMP assay that can eliminate carryover contamination and rapidly detect SARS-CoV-2. The assay can detect the virus as low as 2 copies/μl within 45 minutes and eliminate a significant amount of contaminants. Analysis of clinical samples showed high agreement with rRT-PCR.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Huynh Van Ngoc, Than Linh Quyen, Aaydha Chidambara Vinayaka, Dang Duong Bang, Anders Wolff
Summary: This study developed a fluorescence-based portable detection system for rapid and quantitative detection of the SARS-CoV-2 virus. The system, built on the Arduino platform, demonstrated real-time detection capability and showed excellent agreement with a commercial thermocycler. It has the potential to greatly enhance the practical applicability for on-site screening of the SARS-CoV-2 virus.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Than Linh Quyen, Aaydha Chidambara Vinayaka, Mohsen Golabi, Trieu Nguyen, Huynh Van Ngoc, Dang Duong Bang, Anders Wolff
Summary: In this study, a solid-phase loop-mediated isothermal amplification (SP-LAMP) technique was developed, which integrated with supercritical angle fluorescence (SAF) micro-optic structures to achieve multiplex detection of pathogens. By optimizing key parameters, multiple pathogens were successfully detected and demonstrated good multiplexing capacity. The success of this technique opens up a promising direction for rapid detection of multiple pathogens.
Article
Chemistry, Analytical
Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Than Linh Quyen, Trieu Nguyen, Mohsen Golabi, Mogens Madsen, Dang Duong Bang, Anders Wolff
Summary: Accurate and rapid detection of pathogens in animal-origin foods is crucial for the One Health Action Plan of the EU. We developed a LAMP-based point-of-care device called VETPOD for detecting Salmonella spp. The VETPOD uses a unique cartridge with pyramid-shaped optical structures for light detection, achieving comparable results to the ISO 6579-1 reference method with high sensitivity (96.51%) and specificity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Trieu Nguyen, Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Quyen Than Linh, Sune Zoega Andreasen, Mohsen Golabi, Dang Duong Bang, Jens Kjolseth Moller, Anders Wolff
Summary: This study presents a comprehensive work on the fabrication and clinical validation of a point of care (PoC) device for rapid and onsite detection of SARS-CoV-2. The device, named PATHPOD, can perform the detection of 10 different samples and two controls in less than 50 min, using a real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reaction on a polymer cartridge. The test has comparable sensitivity and specificity to the current golden standard RT-PCR.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biotechnology & Applied Microbiology
Aaydha Chidambara Vinayaka, Than Linh Quyen, Van Ngoc Huynh, Mogens Madsen, Dang Duong Bang, Anders Wolff
Summary: Sensitive detection of pathogens in livestock farms is crucial for the One Health Action Plan of the European Union. A loop-mediated isothermal amplification (LAMP) based veterinary point-of-care device (VETPOD) was developed and tested for detecting Salmonella enterica in primary production samples. Different sampling methods were evaluated, and a two-step centrifugation combined with washing was found crucial for eliminating inhibitors in the faecal matrices. The VETPOD showed promising results, with a high sensitivity and agreement with the reference method.
MICROBIAL BIOTECHNOLOGY
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Mohammad-Ali Shahbazi, Krishna Kant, Joseph John Kaplinsky, Vinayaka Aaydha Chidambara, Dang Duong Bang, Anders Wolff
30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)
(2017)
