Review
Chemistry, Analytical
Michal Gregus, Alexander R. Ivanov, Steven Ray Wilson
Summary: Ultralow flow LC utilizes ultra-narrow bore columns and flow rates ranging from pL/min to low nL/min. It provides high sensitivity and resolution, making it suitable for analyzing scarce biological samples. Recent advancements in dedicated instrumentation, technical approaches, and higher throughput have facilitated the application of ultralow flow LC to the analysis of amount-limited samples. This review discusses the types of ultralow flow LC technology, highlights representative applications in bioanalysis, and explores future developments.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Multidisciplinary Sciences
Brian C. Gau, Andrew W. Dawdy, Hanliu Leah Wang, Bradley Bare, Carlos H. Castaneda, Olga V. Friese, Matthew S. Thompson, Thomas F. Lerch, David J. Cirelli, Jason C. Rouse
Summary: Oligonucleotide mapping using LC-UV-MS/MS is a method developed to support the development of Comirnaty, the first commercial mRNA vaccine against SARS-CoV-2. It allows direct characterization of mRNA's primary structure through enzymatic digestion and mass spectrometry analysis. This technique is pivotal for ensuring the quality, safety, and efficacy of mRNA vaccines.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Fernando de Andres, Angel Rios
Summary: This review discusses the recent advances and applications of carbon dots (CDs) in bioanalytical research and clinical analysis, focusing on their properties, synthetic strategies, characterization techniques, and potential uses with analytical separation approaches. It highlights the importance of CDs as tools in (bio)analytical processes and explores their prospective utilization in nanomaterial applications.
MICROCHEMICAL JOURNAL
(2021)
Review
Electrochemistry
Rumeng Song, Ying Li, Baoxian Ye, Gaiping Li, Lina Zou
Summary: This bibliometric review analyzes articles related to PEC aptasensor published on the Web of Science Core Collection from 2000 to 2022. The study reveals the rapid development of PEC aptasensor in recent years. China has the highest publication volumes and actively cooperates with other countries/regions, particularly the USA. Qingdao University of Science and Technology has the most published articles, while Nanjing University has the highest number of citations. Signal amplification, heterojunction, and antibiotic are identified as the main research directions in this field.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Yue Huang, Si Mou, Yadi Wang, Ruipeng Mu, Meina Liang, Anton Rosenbaum
Summary: Antibody-drug conjugates (ADCs) present challenges in bioanalysis due to their intricate structures and complex metabolism. Traditional measurement methods involve quantifying ADCs and Total Ab, but recent advances in instrumentation, such as high resolution mass spectrometers (HRMS), allow for more precise analysis of large molecules like ADCs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dinghui Xiong, Jie Cheng, Fengxiang Ai, Xinyu Wang, Jiaxuan Xiao, Fang Zhu, Kun Zeng, Kun Wang, Zhen Zhang
Summary: Adsorption of DNA probes onto nanomaterials has great potential for bioassay establishment. However, there is a lack of systematic understanding of the sensing behaviors based on nanomaterial-DNA interactions, limiting design and applications. In this study, metal-organic frameworks (MOFs) were used to investigate the issue. The results revealed the quenching of fluorescently labeled DNA adsorbed onto MOFs and provided insights into the interaction mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Bezhan Chankvetadze
Summary: Separation of enantiomers serves different purposes, with variations in method refinement based on the final application. This overview focuses on the specific requirements of bioanalytical applications, comparing different separation methods and discussing current developments and future trends in the field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Soumya Kandi, John P. Savaryn, Qin C. Ji, Gary J. Jenkins
Summary: The feasibility of using product ions on a high-resolution mass spectrometer for quantification was tested. The in-sample calibration curve (ISCC) approach was found to improve precision in quantifying endogenous peptides from protein digests. High-resolution mass spectrometry allows for isotopic resolution and flexibility in quantification using multiple product ions.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Review
Chemistry, Multidisciplinary
Yang Liu, Jiaojiao Zheng, Wencai Liu, Zhiqin Yuan, Chao Lu
Summary: This review focuses on the recent advances and applications of steady-state and dynamic bioanalysis based on carbon quantum dots (CQDs) as luminescent probes. The synthesis and luminescence mechanism of CQDs are first introduced, followed by the discussion of sensing principles and tactics in CQD-based luminescent bioanalysis. Steady-state bioanalysis systems and dynamic bioanalysis platforms are discussed, and challenges and future trends are also addressed.
Article
Biophysics
Yichen Chen, Biao Ma, Yinxiu Zuo, Gangsheng Chen, Qing Hao, Chao Zhao, Hong Liu
Summary: Wearable sweat bioanalysis has the potential for non-invasive disease diagnostics. However, the collection of representative sweat samples and analysis of clinically significant targets remains challenging. In this study, a versatile method for sweat bioanalysis is presented, utilizing a thermoresponsive hydrogel to absorb sweat without stimulation. The absorbed sweat or preloaded reagents can be released into a microfluidic detection channel through programmed electric heating of the hydrogel modules. Using this method, both one-step glucose detection and multi-step cortisol immunoassay can be achieved within 1 hour, even at low sweat rates. Comparison with conventional blood samples and stimulated sweat samples is performed to evaluate the applicability of this method to non-invasive clinical practice.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Qian Ruan, Kate Comstock
Summary: A novel metabolite profiling workflow was developed using state-of-the-art tribrid high-resolution mass spectrometry system, incorporating advanced data acquisition techniques and data analysis methods to enhance efficiency and coverage of metabolite identification.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Feng-Zao Chen, Zheng Li, Xiang-Nan Liu, Yuan-Cheng Zhu, De-Man Han, Hong-Yuan Chen
Summary: This study presents a novel target-dependent gating-based PEC bioanalysis protocol, which utilizes sandwich immunocomplexation and enzymatic generation of biocatalytic precipitation to regulate the diffusion of the target substance in nanopores. The protocol demonstrates good performance in terms of sensitivity, selectivity, reproducibility, and efficient reutilization of the working electrode, thus providing a potential general PEC analytical platform for various other targets of interest.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Mayank Garg, Nicole Pamme
Summary: Microfluidics has revolutionized measurement science by enabling low sample consumption, fast readout, and high throughput. The incorporation of nanomaterials, particularly graphene analogues, has further enhanced these features, especially in electrochemical analysis systems. However, there has been less research on the use of other graphene analogues for microfluidic biosensors. This review aims to highlight the scientific advancements in analytical chemistry applications related to a wider range of 2-D materials and specifically focuses on microfluidic electrochemical and optical sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Kanchan Sinha Roy, Emir Nazdrajic, Olga Shimelis, M. James Ross, Yong Chen, Hugh Cramer, Janusz Pawliszyn
Summary: Plasma protein binding refers to the binding of a drug to plasma proteins in the body, which is crucial for understanding the free concentration of a drug in the blood for pharmacokinetics and pharmacodynamics studies. Accurate determination of free drug concentrations in the blood is important for therapeutic drug monitoring and personalized medicine. The use of C-18-coated solid-phase microextraction 96-pin devices provides a more efficient method for determining plasma protein binding compared to traditional techniques.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Milos Dvorak, Ondrej Moravcik, Pavel Kuban
Summary: A novel concept for highly versatile automated analyses of dried blood spot (DBS) samples by commercial capillary electrophoresis (CE) is presented in this study. Two interchangeable CE cartridges with different fused-silica capillaries were used for the DBS elutions and the DBS eluate analyses, respectively. The application of two CE cartridges also enabled the employment of CE detectors with different instrumental set-ups and/or principles, such as the optical detection of nonsteroidal anti-inflammatory drugs (NSAIDs) and the conductivity detection of amino acids (AAs).
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Franziska Beck, Carina Horn, Antje J. Baeumner
Summary: Electrochemical immunosensors offer rapid analyte quantification with high sensitivity and selectivity, but user-friendliness can often be a challenge. Using silver nanoparticles instead of gold nanoparticles in POC sensors not only improves sensitivity, but also simplifies assay protocols to meet POC requirements, achieving lower limits of detection in a 2-minute automatic measurement.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Editorial Material
Biochemical Research Methods
Guenter Gauglitz, Antje J. Baeumner
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Simone Rink, Axel Duerkop, Axel Jacobi von Wangelin, Michael Seidel, Antje J. Baeumner
Summary: m-carboxy luminol, with its high hydrophilic design, shows superior performance in H2O2-dependent bioassays, providing increased relative quantum yield and sensitivity, and has the potential to replace luminol as a benchmark probe.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Franziska Beck, Carina Horn, Antje J. Baeumner
Summary: The microfluidic biosensor has key characteristics of desirable home-tests, including low limits of detection, small sample volume, simple detection strategies, and long-term stable reagents. It can accurately assess the severity and progression of diseases.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Simone Rink, Barbara Kaiser, Mark-Steven Steiner, Axel Duerkop, Antje J. Baeumner
Summary: This study demonstrates the use of liposomes to improve the LOD of traditional gold nanoparticle-based assays while maintaining ease of use. Liposomes outperform AuNPs in photometric LFAs, especially in complex matrices like human serum. The technology shows great potential for improving detection of low abundance analytes such as IL-6.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Editorial Material
Biochemical Research Methods
Antje J. Baeumner, Maria C. Moreno Bondi, Sabine Szunerits, Qiuquan Wang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Editorial Material
Biochemical Research Methods
Antje J. Baeumner, Stephen A. Wise
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Meike Bauer, Axel Duerkop, Antje J. Baeumner
Summary: This article critically evaluates the impact of membrane materials and deposition methods on optical and electrochemical sensing systems. It highlights the importance of deposition techniques in determining a sensor's performance. The study also discusses the specific analytes and applications in healthcare diagnostics, food safety, and environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Simone Rink, Antje J. Baeumner
Summary: Point-of-care (POC) diagnostics focus on timely identification of harmful conditions close to patients' needs. While paper-based POC tests, such as lateral flow assays (LFA), have been successful, the COVID-19 pandemic has exposed their limitations. This perspective discusses strategies to develop paper-based POC tests that provide higher sensitivity, objective result interpretation, and multiplexing options for integration with machine learning into digital diagnostics and telemedicine.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Simon Streif, Patrick Neckermann, Clemens Spitzenberg, Katharina Weiss, Kilian Hoecherl, Kacper Kulikowski, Sonja Hahner, Christina Noelting, Sebastian Einhauser, David Peterhoff, Claudia Asam, Ralf Wagner, Antje J. J. Baeumner
Summary: In 2020, the emergence of SARS-CoV-2 led to an increased interest in neutralizing antibody testing. A new platform using RBD-conjugated liposomes was developed for fluorescent high-throughput and point-of-care neutralizing antibody assays. The assays showed good performance with samples of varying neutralizing antibody titers.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Franziska Beck, Michael Loessl, Antje J. J. Baeumner
Summary: Silver nanoparticles (AgNPs) have unique physical, (electro)chemical, and optical properties that make them suitable for sensor and point-of-care (POC) applications. Recent advances have demonstrated that controlling the inherent higher instability toward oxidation of AgNPs can improve sensor performance and enable specific detection of analytes at low concentrations. However, further research is needed to simplify assay procedures and facilitate the successful application of AgNPs in POC settings.
Article
Nanoscience & Nanotechnology
Antonia Perju, Ferdinand Holzhausen, Anna-Maria Lauerer, Nongnoot Wongkaew, Antje J. Baeumner
Summary: This study aims to enhance the sensitivity and quantitative detection capability of point-of-care (POC) devices by directly embedding electrodes within the lateral flow. The electrodes, made from laser-induced carbon nanofibers, enable flow-through and interaction with the entire sample. After optimization, highly sensitive and quantitative detection was successfully achieved.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Florian Gerstl, Uma Pongkitdachoti, Fuangfa Unob, Antje J. Baeumner
Summary: High performance laser-induced graphene (LIG) electrodes were integrated into adhesive tape-based microfluidic channels for electrochemical and electrochemiluminescent detection approaches. This provides low limits of detection, simple hardware requirements, and inexpensive fabrication for point-of-care sensor assays.
Article
Nanoscience & Nanotechnology
Christian Griesche, Kilian Hoecherl, Antje J. Baeumner
Summary: Laser-induced graphene (LIG) is a desirable electrode material for bio- and chemosensors due to its graphene-like characteristics. The study found that the direct pressure-driven transfer of LIG electrodes onto standard polymer substrates produces highly functional transfer-LIG (tLIG) electrodes which outperformed LIG electrodes in some aspects, especially in more advanced microfluidic channel systems. LIG and tLIG have the potential to change electroanalytical sensing in diagnostic systems with their scalable and easy-to-integrate fabrication process.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Cornelia A. Hermann, Michael Mayer, Christian Griesche, Franziska Beck, Antje J. Baeumner
Summary: The study introduces a microfluidic method for labeling functional nanovesicles, avoiding the risk of conglomerate formation commonly observed in bulk reactions. Magnetic properties are beneficial for nanoparticles in improving separation capabilities.
