Article
Chemistry, Applied
Peng Sun, Zhao Yu, Chao Wang, Shuo Li
Summary: In this study, the content of eight sterols in rapeseed, including cholesterol, cholestanol, brassicasterol, ergosterol, campesterol, stigmasterol, 8-sitosterol, and stigmastanol, was determined using pressurized liquid extraction coupled with gas chromatography-mass spectrometry. The results showed a good linear relationship between the peak area and the corresponding mass concentration of the eight sterols. The developed method was efficient, simple, and accurate, suitable for the qualitative and quantitative analysis of sterols in rapeseed.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Review
Chemistry, Multidisciplinary
Ting Ruan, Pengyang Li, Haotian Wang, Tingyu Li, Guibin Jiang
Summary: Exposure to environmental organic pollutants has resulted in significant ecological impacts and negative health outcomes, leading to increased attention. Identifying the contribution of unknown chemical components is a crucial knowledge gap in understanding the combined effects of pollutant mixtures. Recent analytical breakthroughs have addressed this issue, and various complementary analytical methodologies and experimental platforms have been developed. Future research directions include nontarget screening methods, interdisciplinary techniques for toxicant identification, and strategies for risk assessment of chemical cocktails.
Review
Environmental Sciences
Carlos Castillo-Zacarias, Mario E. Barocio, Enrique Hidalgo-Vazquez, Juan Eduardo Sosa-Hernandez, Lizeth Parra-Arroyo, Itzel Y. Lopez-Pacheco, Damia Barcelo, Hafiz N. M. Iqbal, Roberto Parra-Saldivar
Summary: Antidepressants contaminate urban and non-urban water bodies, leading to bioaccumulation in aquatic animals. This contamination is expected to increase due to rising depression and anxiety levels during the COVID-19 pandemic.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Medical Laboratory Technology
Eun Jeong Won, Ahram Yi, Young Jin Ko
Summary: In this study, a highly sensitive and derivatization-free LC-MS/MS method was successfully developed for measuring estradiol levels in patients with estradiol-related endocrine disorders. The method showed good analytical performance and comparable results to a chemiluminescent microparticle immunoassay.
CLINICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jakob Morgenstern, Thomas Fleming, Elisabeth Kliemank, Maik Brune, Peter Nawroth, Andreas Fischer
Summary: This study developed a sensitive and specific method to quantify all-trans retinoic acid (atRA) in human plasma samples, which can also be applied to hyperlipidemic samples. The method showed significant differences in atRA plasma levels between patients with different types of diseases, and the concentration of atRA was associated with lipoprotein levels when compared to healthy controls.
Article
Biochemical Research Methods
V Fernandez-Fernandez, M. Ramil, R. Cela, I Rodriguez
Summary: A modular solid-phase extraction (SPE) setup was developed to effectively extract neutrals, acids, and bases micro pollutants from wastewater. The approach showed excellent performance in extracting 64 compounds and identified 19 pollutants with low removal efficiencies during wastewater treatment or even enhancement.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Songlin Yu, Danchen Wang, Xiaoli Ma, Yuanyuan Zhang, Dandan Sun, Yutong Zou, Jialei Yu, Fang Zhang, Guohua Yang, Shaowei Xie, Qian Cheng, Xinqi Cheng, Ling Qiu
Summary: A LC-MS/MS method was established to quantify G17 and G34 simultaneously, showing high analytical performance. The method was compared with CLIA and RIA, and it was found to be highly correlated with both methods. The study suggests that G17 may be the most specific biomarker for gastrinoma diagnosis.
CLINICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Marina Pereira-Coelho, Isabel Cristina da Silva Haas, Cassia Katrin Reinke, Jocinei Dognini, Renata Dias de Mello Castanho Amboni, Luciano Vitali, Luiz Augusto dos Santos Madureira
Summary: A simple sample preparation approach employing the dispersive pipette extraction (DPX) technique is proposed to determine twelve polyphenols in wines. The extraction parameters were optimized using univariate and multivariate designs. The analytical performance was satisfactory with high determination coefficients and good precision.
Article
Biochemical Research Methods
Ming Huang, Xiaobin Xu, Haibo Qiu, Ning Li
Summary: In this study, a generic HILIC-MS/MS method was developed for in-depth characterization of nucleic acid therapeutic modalities. High-resolution mass spectrometry was used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities. Optimized higher-energy C-trap dissociation was used to improve the sequence coverage of DNA and RNA oligonucleotides, achieving higher on-column sensitivity for phosphorothioate oligonucleotide analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Miguel Cobo Golpe, Gabriela Castro, Maria Ramil, Rafael Cela, Ysabel Santos, Isaac Rodriguez
Summary: This study optimized and validated a method based on MSPD and LC-MS/MS for the sensitive and selective determination of chlorhexidine in sludge from STPs. Analysis of samples from different STPs in Northwest Spain revealed the presence of chlorhexidine, which may impact microbial populations but is unlikely to affect plant germination.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Seunghwa Lee, Kang Mi Lee, Sang Moon Han, Hyeon-Jeong Lee, Changmin Sung, Hophil Min, Hosub Im, Sang Beom Han, Sangwon Cha, Jaeick Lee
Summary: To assess the combined effects of environmental pollutants (EPOLs), a comprehensive analytical method was developed to simultaneously analyze multiple EPOLs. The method overcame technical challenges associated with enzyme hydrolysis, simultaneous extraction, and analysis. The method was validated and showed potential for long-term, large-scale, and data-rich human biomonitoring of EPOLs.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Wen Ma, Jun Li, Xianjiang Li, Huwei Liu
Summary: A series of metal-organic frameworks were synthesized and used as adsorbents for five diamide insecticides, with MIL-101-NH2 showing superior performance. A sensitive method combining dispersive solid phase extraction with liquid chromatography tandem mass spectrometry was developed, achieving high enrichment efficiency by investigating key parameters. The proposed method demonstrated ultrahigh sensitivity and wide linearity for the analysis of diamide insecticides.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Plant Sciences
Cheng Song, Yunpeng Zhang, Muhammad Aamir Manzoor, Guohui Li
Summary: Jasmonate signaling is important for plant stress responses and secondary metabolism. This study established a rapid identification method for alkaloids in Dendrobium officinale and identified seven alkaloids and forty-nine other chemical substances in D. officinale. These findings provide insights into the composition of alkaloids in Dendrobium plants and offer a scientific method for their identification.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Amelie Chabilan, Nicolette Landwehr, Harald Horn, Ewa Borowska
Summary: The quantification of antibiotics in sediments is challenging, but necessary to understand their fate in aquatic systems. In this study, a comprehensive method was developed to determine 19 antibiotics in river sediments. By considering the physical-chemical properties of the analytes, a new classification method based on log(Kow) values was proposed to select suitable extraction solvents. After comparing different extraction protocols, an effective method using acetonitrile and phosphoric acid achieved high recovery rates for antibiotics with log(Kow) values above zero.
Article
Biochemical Research Methods
Jufang Li, Aiping Xu, Jianyou Xue, Wei Qian, Ping Xu, Zhineng Hu, Chen Chen, Chaochao Wu
Summary: The paper proposes a novel method of deep eutectic solvent (DES)-assisted liquid-liquid extraction (LLE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for extracting steroid hormones from serum. The method has good linear calibration ranges, high extraction recoveries, and low limits of quantification, making it a simple and environmentally-friendly method.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Agriculture, Multidisciplinary
C. Pelosi, C. Bertrand, G. Daniele, M. Coeurdassier, P. Benoit, S. Nelieu, F. Lafay, V. Bretagnolle, S. Gaba, E. Vulliet, C. Fritsch
Summary: Research shows that the exposure to multiple classes of currently used pesticides poses a threat to earthworms in arable landscapes, with a high percentage of soils and earthworms contaminated at levels that could endanger these beneficial soil organisms. Chronic toxicity to earthworms was found in both treated winter cereals and untreated habitats, indicating potential negative impacts on biodiversity, recovery, and ecosystem functions. These results highlight the importance of regulating pesticides and promoting sustainable agriculture practices to address global environmental challenges.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2021)
Article
Geochemistry & Geophysics
Gwenaelle Corbin, Emmanuelle Vulliet, Bruno Lanson, Albert Rimola, Pierre Mignon
Summary: The study found that the adsorption of carbamazepine and paracetamol onto expandable clay minerals is low, with neither compound entering the interlayer space of the clay. The adsorption mainly occurs through van der Waals interactions on the clay surface.
Article
Environmental Sciences
S. Joachim, R. Beaudouin, G. Daniele, A. Geffard, A. Bado-Nilles, C. Tebby, O. Palluel, O. Dedourge-Geffard, M. Fieu, M. Bonnard, M. Palos-Ladeiro, C. Turies, E. Vulliet, V David, P. Baudoin, A. James, S. Andres, J. M. Porcher
Summary: This study found that diclofenac had significant adverse effects on aquatic organisms, including water plants, benthic animals, and fish, in a long-term freshwater mesocosm experiment. The effects observed in more natural conditions were more severe compared to those seen in laboratory settings. The implications of these findings for regulatory purposes were also discussed.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Engineering, Environmental
Ronan Guillossou, Julien Le Roux, Angelique Goffin, Romain Mailler, Gilles Varrault, Emmanuelle Vulliet, Catherine Morlay, Fabrice Nauleau, Sabrina Guerin, Vincent Rocher, Johnny Gasperi
Summary: This study investigated the potential of fluorescence spectroscopy in monitoring the removal of organic micropollutants (OMPs) by activated carbon (AC) adsorption in industrial scale wastewater treatment. Different operational conditions and physico-chemical properties of OMPs affected the removal efficiency. Positive correlations were found between fluorescence indexes and OMP removal, with higher correlation coefficients compared to UV254, indicating the promising application of fluorescence spectroscopy.
Article
Multidisciplinary Sciences
Bastian Schulze, Denice van Herwerden, Ian Allan, Lubertus Bijlsma, Nestor Etxebarria, Martin Hansen, Sylvain Merel, Branislav Vrana, Reza Aalizadeh, Bernard Bajema, Florian Dubocq, Gianluca Coppola, Aurelie Fildier, Pavla Fialova, Emil Frokjaer, Roman Grabic, Pablo Gago-Ferrero, Thorsten Gravert, Juliane Hollender, Nina Huynh, Griet Jacobs, Tim Jonkers, Sarit Kaserzon, Marja Lamoree, Julien Le Roux, Teresa Mairinger, Christelle Margoum, Giuseppe Mascolo, Emmanuelle Mebold, Frank Menger, Cecile Miege, Jeroen Meijer, Regis Moilleron, Sapia Murgolo, Massimo Peruzzo, Martijn Pijnappels, Malcolm Reid, Claudio Roscioli, Coralie Soulier, Sara Valsecchi, Nikolaos Thomaidis, Emmanuelle Vulliet, Robert Young, Saer Samanipour
Summary: Non-target analysis using high-resolution mass spectrometry is commonly used to detect emerging chemicals in complex environmental samples. Inter-laboratory studies help evaluate different workflows and establish standardized quality control procedures. The data generated from this study serves as a valuable resource for future development of algorithms and workflows in NTA experiments.
Article
Environmental Sciences
Vincent Dufour, Laure Wiest, Sylvain Slaby, Francois Le Cor, Lucile Auger, Olivier Cardoso, Laurence Curtet, Laure Pasquini, Xavier Dauchy, Emmanuelle Vulliet, Damien Banas
Summary: Monitoring pesticides in small organisms in the environment requires sensitive analytical methods. This study developed a miniaturized extraction protocol for small biotic samples, allowing for the characterization of multiple pesticides and transformation products. The method showed high extraction rates and recoveries, making it an effective tool for analyzing polar pesticides in small organisms.
Article
Environmental Sciences
Sylvain Slaby, Francois Le Cor, Vincent Dufour, Lucile Auger, Laure Pasquini, Olivier Cardoso, Laurence Curtet, Jean-Marc Baudoin, Laure Wiest, Emmanuelle Vulliet, Cyril Feidt, Xavier Dauchy, Damien Banas
Summary: The study revealed that small waterbodies at the head of basins are impacted by agricultural contaminants, with higher concentrations of transformation products compared to parent compounds. Multiple contaminants were detected in water and sediment, with a few accumulating in fish species.
ENVIRONMENTAL POLLUTION
(2022)
Article
Multidisciplinary Sciences
Emmanuelle Lebeau-Roche, Gaelle Daniele, Aurelie Fildier, Cyril Turies, Odile Dedourge-Geffard, Jean-Marc Porcher, Alain Geffard, Emmanuelle Vulliet
Summary: Environmental metabolomics aims to comprehensively analyze the metabolites in organisms to reveal biochemical pathways influenced by stressors and potential biomarkers. This study introduces a methodology for untargeted metabolomics in the three-spined stickleback, evaluating differences and complementarities among brain, gills, liver, and whole fish matrices.
Article
Polymer Science
Ibtissem Jlalia, Taha Chabbah, Saber Chatti, Frederic Schiets, Herve Casabianca, Catherine Marestin, Regis Mercier, Steffen M. Weidner, Hans R. Kricheldorf, Abdelhamid Errachid, Emmanuelle Vulliet, Mohamed Hammami, Nicole Jaffrezic-Renault
Summary: The study aimed to design new sorbent phases and found that P6 co-poly(ether-pyridine) has higher adsorption efficiency, with Langmuir model showing better fitting of the sorption isotherms.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet
Summary: The use of beta-lactams in veterinary and human medicine has increased in the past decade, representing a significant portion of overall consumption. This study developed a modified QuEChERS method for extraction and analysis of beta-lactams from sewage treatment plant sludge, showing satisfactory performance for quantification and detection. Through optimization and validation, the method proved effective in extracting beta-lactams from solid environmental matrices.
Article
Chemistry, Analytical
Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet
Summary: In this study, an enhanced method for analyzing five beta-lactams in wastewater treatment plant sludge was proposed, using a hybrid triple quadrupole-Linear Ion Trap spectrometer for triple stage MS acquisition. The method achieved improved sensitivity and specificity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Marine & Freshwater Biology
Corentin Mit, Anne Bado-Nilles, Gaelle Daniele, Barbara Giroud, Emmanuelle Vulliet, Remy Beaudouin
Summary: Bisphenol A (BPA) is a concerning chemical due to its endocrine disrupting function, high production volume, and persistence in aquatic environment. A physiologically-based toxicokinetic (PBTK) model was modified to accurately simulate the fate of BPA in fish. The experiments showed that BPA metabolism in fish is not only limited to the liver, but plasma and gills may also play a role.
AQUATIC TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Laure Wiest, Barbara Giroud, Maeva Fieu, Azziz Assoumani, Francois Lestremau, Emmanuelle Vulliet
Summary: Despite their wide use in various fields, the knowledge about surfactants in environmental solid matrices is generally limited. Aiming to develop an extraction method in sediments for the simultaneous analysis of anionic, cationic, and non-ionic surfactants, this study compared different extraction techniques, solvents, and additives. The optimized method, coupled with liquid chromatography and tandem mass spectrometry, was validated and applied to real samples, allowing the detection of 18 out of 27 targeted surfactants.
Article
Chemistry, Multidisciplinary
Paul Rocchi, Lucie Labied, Tristan Doussineau, Michel Julien, Barbara Giroud, Emmanuelle Vulliet, Jerome Randon, Olivier Tillement, Agnes Hagege, Francois Lux
Summary: Ultrasmall inorganic nanoparticles have gained significant attention in recent years for their beneficial biodistribution, pharmacokinetics, and theranostic properties. AGuIX nanoparticles made of polysiloxane and gadolinium chelates have been successfully translated for clinical use. In aqueous mediums, these nanoparticles are in dynamic equilibrium with polysiloxane fragments due to the hydrolysis of Si-O-Si bonds. High-performance liquid chromatography coupled with electrospray ionization mass spectrometry has enabled the separation and identification of these fragments.
Article
Engineering, Environmental
Imen Abdelhadi-Miladi, Taha Chabbah, Saber Chatti, Houyem Abderrazak, Patrice Saint-Martin, Herve Casabianca, Catherine Marestin, Regis Mercier, Hatem Ben Romdhane, Emmanuelle Vulliet, Abdelhamid Errachid, Nicole Jaffrezic-Renault
Summary: Four semi-interpenetrating networks (semi-IPN) based on polydimethylsiloxane (PDMS) were developed for the sorptive extraction of polar phenolic compounds. Physico-chemical properties of the semi-IPN were controlled and optimized, resulting in good extraction efficiency for target analytes.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
