期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 398, 期 6, 页码 2373-2382出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3848-8
关键词
Microparticle; Flow cytometry; Microfluidics; Microflow cytometer; Biosensors
资金
- NIH [UO1 A1075489]
Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.
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