Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Fabio de Souza Barbosa, Leonardo Capra Pezzi, Favero Reisdorfer Paula, Sarah Eller, Elfrides E. S. Schapoval, Tiago Franco de Oliveira, Andreas S. L. Mendez
Summary: The stability of the antibiotic doripenem was evaluated by LC-ESI-Q-TOF method, revealing six degradation products under stress conditions and the formation of a major degradation product through cleavage of the beta-lactam ring under hydrolytic conditions. Additionally, oxidation of the drug using H2O2 led to the formation of three new degradation products. The study provides insight into the degradation behavior of doripenem and its important antibiotic class.
MICROCHEMICAL JOURNAL
(2021)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Construction & Building Technology
Stepanka Kuckova, Gabriela Rambouskova, Petra Junkova, Jiri Santrucek, Pavel Cejnar, Tatiana Anatolievna Smirnova, Ondrej Novotny, Radovan Hynek
Summary: The enrichment of mortar mixtures with natural organic materials has a long history, with protein additives proven to enhance mortar durability and strength. This study aimed to identify and quantify proteins in model mortars with various protein additives using mass spectrometry techniques. The results confirmed the possibility of identifying protein additives in century-old mortars, shedding light on traditional recipes.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Food Science & Technology
Yingying Zheng, Long Tang, Mingguang Yu, Ting Li, Huanlu Song, Pei Li, Ku Li, Jian Xiong
Summary: Research should focus on developing novel agents to replace salt in foods without affecting taste, to avoid health risks associated with high-salt diets. Some yeast extracts can be used as salt substitutes without imparting additional odors. Salty peptides were fractionated from Angel Yeast in this study.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Ophthalmology
Ai Lin Daphne Teh, Jaime Jacqueline Jayapalan, Mun Fai Loke, Azida Juana Wan Abdul Kadir, Visvaraja Subrayan
Summary: This study used a non-targeted metabolomics approach to investigate metabolite differences between patients with keratoconus and control subjects, identifying potential serum biomarkers. The identified metabolites, including dehydroepiandrosterone sulfate and eicosanoids, showed significant upregulation in keratoconus patients. Glycerophospholipid PS(17:2(9Z,12Z)/20:4 (5Z,8Z,11Z,14Z)) was significantly upregulated in control subjects. These differentially regulated metabolites provide insights into the pathophysiology of keratoconus and could potentially be utilized as biomarkers for early diagnosis and monitoring of the disease.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Food Science & Technology
Latifun Jayanti, Dewi Yuliyanti, Zhaoqi Zhan, Peiling Hou, Karyanto E. Mulyono, Paul Wassell
Summary: This study focuses on the analysis of triacylglycerol (TAG) composition in Moringa oleifera seed oil (MOSO). A new method combining reverse-phase high-performance liquid chromatography (HPLC) and mass spectrometry was used to identify and determine the TAG compositions. Approximately 13.9% of the TAG compositions in MOSO were still unknown. The new method successfully characterized a total of 33 TAG compositions, with triolein (OOO) being the dominant TAG at 39.3%.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Energy & Fuels
Nushrat Yeasmen, Valerie Orsat
Summary: A maceration process was optimized to extract phenolic compounds from sugar maple leaves. High-speed homogenization was found to be an effective pre-treatment. The study identified the optimal conditions for extraction and characterized the extract. LC-ESI-Q-TOF-MS/MS analysis identified 81 phenolic compounds and proposed a gallotannins pathway.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Horticulture
Luana Beatriz dos Santos Nascimento, Antonella Gori, Ilaria Degano, Alessandro Mandoli, Francesco Ferrini, Cecilia Brunetti
Summary: The study compared the efficiency of fermentation extraction with ultrasound-assisted extraction on the yield of antioxidant polyphenols from elderberry fruits and pomegranate fruit-peels. Results showed that ultrasound-assisted extraction was more suitable for extracting polyphenols from both fruits and exhibited higher antioxidant capacities.
Article
Biochemical Research Methods
Roshan M. Borkar, Abhinav Kanwal, Bandu Raju, Sai Sharanya Pulimamidi, Agneesh Pratim Das, Subhash Mohan Agarwal, Sanjay K. Banerjee, Ragampeta Srinivas
Summary: Phlorizin (PRZ) is a natural dihydrochalcone that blocks glucose absorption by inhibiting sodium/glucose cotransporters (SGLTs) in the intestine and kidney. It can be used as an adjuvant treatment for type 2 diabetes.
JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Cell Biology
Chandranayaka Siddaiah, Anil Kumar BM, Saligrama Adavigowda Deepak, Syed Salman Lateef, Saurabh Nagpal, Kanchugarakoppal S. Rangappa, Chakrabhavi D. Mohan, Shobith Rangappa, Madan S. Kumar, Minaxi Sharma, Vijai Kumar Gupta
Summary: This study focused on metabolite profiling and identification from the bark of Alangium salviifolium by using organic and aqueous solvents. Eighty-one secondary metabolites with therapeutic potential were identified, showcasing the therapeutic value of this plant.
Article
Chemistry, Analytical
Sylvia K. Neef, Ute Hofmann, Thomas E. Muerdter, Matthias Schwab, Mathias Haag
Summary: The suitability of a capillary hydrophilic interaction chromatography (CapHILIC) -QTOF-MS system for non-targeted metabolomics was evaluated and compared with a narrow-bore hydrophilic interaction chromatography (HILIC) -QTOF-MS system. Both platforms detected a similar number of features and performed well in terms of retention time stability, analytical repeatability, and signal intensity. The use of CapHILIC increased the signal areas of all metabolites, though the signal-to-noise ratio was only improved for 50% of the metabolites. Further optimization of CapHILIC conditions improved reproducibility and signal intensity.
Article
Biotechnology & Applied Microbiology
Marcos Edgar Herkenhoff, Luiz A. Bovolenta, Oliver Broedel, Lucilene D. dos Santos, Arthur C. de Oliveira, Luiz G. A. Chuffa, Amanda de O. Ribeiro, Luiz A. Lupi, Marco A. D. Dias, Alexandre W. S. Hilsdorf, Marcus Frohme, Danillo Pinhal
Summary: This study characterized the miRNA and protein profiles of Nile tilapia crossbreed using high throughput sequencing and mass spectrometry techniques, revealing distinctive features in gene expression and protein composition between the crossbreed and its parental strains. These unique miRNA and protein signatures contribute to the phenotype of the crossbreed.
Article
Chemistry, Analytical
Shuang Wu, Hui-Min Li, YI-Fan Bing, Yan Zheng, Wen-Lan Li, Xiang Zou, Zhong-Yuan Qu
Summary: The paper investigates the antidepressant quality markers (Q-markers) of Bupleurum scorzonerifolium (BS) using UPLC-ESI-Q-TOF-MS. SSa, SSc, and SSd were confirmed as Q-markers of BS, and the contents of these markers in 10 batches of BS from different origins were determined. This study provides a reliable method for the quality evaluation of BS for depression treatment.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Lucia Citores, Mariangela Valletta, Vikram Pratap Singh, Paolo Vincenzo Pedone, Rosario Iglesias, Jose Miguel Ferreras, Angela Chambery, Rosita Russo
Summary: Penicillium digitatum is a common pathogen that causes postharvest decay of citrus. Chemical fungicides are currently the main control strategy, but the emergence of fungicide-resistant strains necessitates the exploration of new alternatives. Understanding the molecular response of P. digitatum to biological and chemical antifungal agents can contribute to the development of safer and more effective non-chemical control methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Nicola Landi, Angela Clemente, Paolo V. Pedone, Sara Ragucci, Antimo Di Maro
Summary: This article reports the current status of bioactive peptides isolated and characterized from mushrooms in the past 20 years. It summarizes the classification, biological activities, isolation principles, and sources of these peptides. It was found that some peptides were misclassified, with certain fungal proteins being mistakenly categorized as peptides. Furthermore, this review highlights the various biological activities exhibited by these peptides.
Article
Biochemistry & Molecular Biology
Rossella Rotondo, Sara Ragucci, Salvatore Castaldo, Nicola Landi, Maria Antonietta Oliva, Paolo Pedone, Antimo Di Maro, Antonietta Arcella
Summary: In this study, Ageritin, the prototype of the ribotoxin-like protein family, was found to inhibit the growth of primary human glioblastoma cell lines and enhance sensitivity to the chemotherapy drug temozolomide. It was also observed to cause genotoxicity through the formation of micronuclei, damaging DNA and downregulating the MGMT DNA repair protein. These findings suggest that Ageritin could be a potential innovative treatment for glioblastoma.
Article
Biochemistry & Molecular Biology
Ersilia Nigro, Maria Tommasina Pecoraro, Marialuisa Formato, Simona Piccolella, Sara Ragucci, Marta Mallardo, Rosita Russo, Antimo Di Maro, Aurora Daniele, Severina Pacifico
Summary: CBDA, as the main precannabinoid in industrial hemp, has abundant content in hemp seed oil and wasted leaves, shows low cytotoxicity and can reduce the release of proinflammatory cytokines and chemokines.
Article
Biochemistry & Molecular Biology
Sara Ragucci, Pasqualina Woodrow, Angela Clemente, Rosita Russo, Mariangela Valletta, Nicola Landi, Luigi Russo, Angela Chambery, Antimo Di Maro
Summary: In this study, the characterization, amino acid sequence determination, and functional comparison of myoglobins (Mbs) from Atlantic mackerel and Tinker mackerel were reported. The results showed differences in autoxidation rate and pseudoperoxidase activity between the two Mbs, which were supported by amino acid sequence and structural analysis. The phylogenetic analysis confirmed that Atlantic mackerel and Tinker mackerel are distinct species. Additionally, an analytical method was developed to distinguish between the two mackerel species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Stefano Tomassi, Caterina Ierano, Alessandra Del Bene, Antonia D'Aniello, Maria Napolitano, Giuseppina Rea, Federica Auletta, Luigi Portella, Anna Capiluongo, Vincenzo Mazzarella, Rosita Russo, Angela Chambery, Stefania Scala, Salvatore Di Maro, Anna Messere
Summary: Synthetic nucleic acid interactors have exciting biotechnological and potential therapeutic applications. This study focuses on the synthesis of homo-thymine nucleopeptides and investigates the influence of underivatized amino acid residues and the distance between the nucleobase and peptide backbone on nucleic acid recognition. The results show that two of the nucleopeptides can efficiently bind DNA and RNA targets and cross membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Nicola Landi, Maja Grundner, Sara Ragucci, Miha Pavsic, Martina Mravinec, Paolo Pedone, Kristina Sepcic, Antimo Di Maro
Summary: This study reports the characterization and cytotoxic effects of four novel ribotoxin-like proteins (RL-Ps), called eryngitins, found in the king oyster mushroom. These proteins show enzymatic activity and exhibit cytotoxic action against insect cells, suggesting their potential use as bioinsecticides in biotechnological applications.
Article
Food Science & Technology
Nicola Landi, Vincenza Ciaramella, Sara Ragucci, Angela Chambery, Fortunato Ciardiello, Paolo V. Pedone, Teresa Troiani, Antimo Di Maro
Summary: This study aimed to improve the therapeutic action of cetuximab by conjugating it with the RIP quinoin isolated from quinoa seeds. The novel quinoin cetuximab immunoconjugate showed significant dose-dependent cytotoxicity against cetuximab-resistant cells, suggesting its potential as a candidate for targeted therapy in the treatment of cetuximab-resistant metastatic colorectal cancer.
Article
Biochemistry & Molecular Biology
Sara Ragucci, Hafiza Zumra Fatima Hussain, Andrea Bosso, Nicola Landi, Angela Clemente, Paolo Vincenzo Pedone, Elio Pizzo, Antimo Di Maro
Summary: White button mushroom is a widely consumed edible mushroom with rich nutrients and bioactive molecules. A novel ribotoxin-like protein (RL-P) with specific ribonucleolytic activity has been purified and characterized from this mushroom, showing lower thermal stability compared to RL-Ps from other mushrooms.
Article
Biochemistry & Molecular Biology
Lucia Citores, Sara Ragucci, Rosita Russo, Claudia C. Gay, Angela Chambery, Antimo Di Maro, Rosario Iglesias, Jose M. Ferreras
Summary: We have isolated and purified a cytotoxic protein called Ledodin from shiitake mushrooms, which consists of a 197 amino acid chain. Ledodin showed N-glycosylase activity on mammalian 28S rRNA and inhibited protein synthesis, but had no effect on insect, fungal, and bacterial ribosomes. It is believed to have a catalytic mechanism similar to that of DNA glycosylases and plant ribosome-inactivating proteins. Ledodin could be the first representative of a new family of enzymes widely distributed among basidiomycetes. These proteins are of interest due to their potential toxicity in edible mushrooms and their applications in medicine and biotechnology.
Article
Food Science & Technology
Mariangela Valletta, Nicoletta Campolattano, Ida De Chiara, Rosangela Marasco, Vikram Pratap Singh, Lidia Muscariello, Paolo Vincenzo Pedone, Angela Chambery, Rosita Russo
Summary: Due to the increasing consumer attention to functional foods with probiotics, the market for fermented milk beverage Kefir is growing. There is a demand for new methods to certify the authenticity of Kefir products. In this study, an innovative nanoLC-ESI-MS/MS-based methodology was developed to profile lactic acid bacteria in Kefir products and grains at different levels. This methodology enables the authentication of probiotic starters and provides insights into the composition of lactic acid bacteria in unlabelled Kefir, highlighting the potential of LC-MS/MS as a sensitive tool for assessing the authenticity of fermented foods.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Sara Ragucci, Stefany Castaldi, Nicola Landi, Rachele Isticato, Antimo Di Maro
Summary: Ageritin from poplar mushrooms is an antifungal enzyme that inhibits the growth of various fungi affecting crops. It specifically hydrolyzes a phosphodiester bond in the large rRNA, blocking protein synthesis. The antifungal activity of ageritin was demonstrated against Botrytis cinerea, Colletotrichum truncatum, and Alternaria alternata, while no effect was observed on Trichoderma harzianum. In vitro studies revealed that ageritin can damage fungal ribosomes, and further experiments in liquid medium confirmed its concentration-dependent antifungal effect on B. cinerea and T. harzianum.
Article
Food Science & Technology
Nicola Landi, Laura Alberico, Angela Clemente, Stefania Peddio, Hafiza. Z. F. Hussain, Sara Ragucci, Paolo Zucca, Pasqualina Woodrow, Antimo Di Maro
Summary: Italy is rich in uncharacterized common bean landraces. This study evaluated the composition and profiles of 'Cerato' and 'Curniciello' dry beans cultivated in Caserta, Southern Italy, and compared them with other local dry beans. 'Cerato' beans had lower levels of proteins, lipids, and amino acids compared to 'Curniciello' beans. Fatty acid analysis showed that linoleic acid, alpha-linolenic acid, and oleic acid were the most abundant in both beans. The inhibitory activities of enzymes related to protein and carbohydrate intake were also investigated. AFLP analysis provided useful information for authentication and traceability. Overall, this study contributes to the preservation of local culinary traditions and Italian biodiversity.
Article
Microbiology
Michela Di Napoli, Brigida Silvestri, Giusy Castagliuolo, Andrea Carpentieri, Giuseppina Luciani, Antimo Di Maro, Sergio Sorbo, Alessandro Pezzella, Anna Zanfardino, Mario Varcamonti
Summary: In this study, a fungal species, Cladosporium halotolerans, is isolated from Galleria mellonella larvae. It is found that this fungus is capable of interacting with and degrading polyethylene.
FEMS MICROBIOLOGY ECOLOGY
(2023)