Review
Food Science & Technology
Carolyn W. Kinkade, Zorimar Rivera-Nunez, Ludwik Gorcyzca, Lauren M. Aleksunes, Emily S. Barrett
Summary: Research suggests that mycotoxins like Zearalenone can have adverse effects on the female reproductive system, including infertility, ovarian disorders, and pregnancy complications.
Review
Food Science & Technology
Adam Pierzgalski, Marcin Bryla, Joanna Kanabus, Marta Modrzewska, Grazyna Podolska
Summary: Mycotoxins, such as DON and ZEN, have detrimental effects on human and animal health by causing DNA damage, disrupting the cell cycle, and altering gene expression. These toxins can also impact neurotransmitters and modify DNA through various mechanisms, potentially contributing to the development of metabolic diseases.
Review
Engineering, Chemical
Na Wu, Wen Ou, Zhidong Zhang, Yuwen Wang, Qing Xu, He Huang
Summary: Zearalenone (ZEN) is a widespread mycotoxin that contaminates crops and animal feed, causing serious health problems for livestock and humans. Biological methods, especially microbial degradation and enzyme extraction, offer promising solutions for ZEN decontamination without sacrificing nutritional quality or safety concerns.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Food Science & Technology
Krisztina Majer-Baranyi, Attila Barocsi, Patrik Gadoros, Laszlo Kocsanyi, Andras Szekacs, Nora Adanyi
Summary: A capillary-based immunofluorescence sensor was developed and integrated into a flow injection analysis system. The system achieved high sensitivity by efficiently collecting and detecting non-guided fluorescence signals scattered along the capillary wall. The sensor demonstrated excellent performance in quantitative determination, with low detection limits and a wide dynamic detection range.
Article
Food Science & Technology
Inna Szekacs, Nora Adanyi, Istvan Szendro, Andras Szekacs
Summary: Novel OWLS-based immunosensor formats were developed for label-free detection of ZON, achieving low LODs with immobilized antibody- and antigen-based assays. The competitive immunosensor setup showed an outstanding LOD of 0.002 pg/mL for ZON with a dynamic detection range between 0.01 and 1 pg/mL, demonstrating significant improvement in detectability relative to ELISA.
Article
Chemistry, Analytical
Weili Qiao, Baoshan He, Wenjie Ren, Renyong Zhao, Zhiguang Suo, Haoyang Yan, Yiwei Xu, Min Wei, Huali Jin
Summary: In this study, colloidal Au spheres and Au nanoflowers were used as signal labels to detect ZEN in cereals. The sensitivity of the immunochromatographic strip was optimized by adjusting the volume of antigen, antibody, and probe quantities. The use of Au nanoflowers as signal labels showed higher sensitivity compared to traditional colloidal Au spheres, with a low limit of detection of 0.08 ng mL(-1). The Au nanoflowers immunochromatographic strip demonstrated high specificity and sensitivity for ZEN detection.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Haixu Zhao, Shang Ren, Zhenzhe Wei, Xinhui Lou
Summary: In this study, a novel optical-fiber aptasensor was developed for the detection of Zearalenone (ZEN), a common mycotoxin pollutant in agricultural products. The aptasensor exhibited unprecedented sensitivity, high specificity, low-cost, and easy of automation, enabling the detection of ZEN at sub-picomolar levels. The sensor also demonstrated excellent specificity and regeneration capability, making it a promising tool for practical applications.
Article
Microbiology
Alexander A. Stakheev, Denis Erokhin, Ekaterina A. Meleshchuk, Oleg D. Mikityuk, Natalia Statsyuk
Summary: This study reveals that the main pathway for inhibition of DON and ZEN biosynthesis is the transcriptional regulation of genes involved in the G-protein-cAMP-PKA signaling pathway. These findings contribute to a better understanding of the genetic mechanisms underlying mycotoxin production and its regulation.
Article
Food Science & Technology
Alexei Nabok, Ali Madlool Al-Jawdah, Borbala Gemes, Eszter Takacs, Andras Szekacs
Summary: The PW immunosensor developed as a polarisation interferometer successfully detected ZON by detecting the phase shift of polarised light passing through the PW. By immobilising ZON-specific antibodies on the waveguide and adsorbing protein A, the sensor could concentration-dependently detect ZON concentrations in the sample solution up to 1000 ng/mL, with a detection limit of 0.01 ng/mL.
Review
Biochemical Research Methods
Xhensila Shkembi, Marketa Svobodova, Vasso Skouridou, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Ciara K. O'Sullivan
Summary: This review discusses the selection of aptamers against mycotoxins and their incorporation into optical and electrochemical aptasensors. The strategies, advantages, and disadvantages of each approach are outlined, along with the different materials used and immobilization methods employed to achieve the highest sensitivity and selectivity.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Microbiology
Liwen Zhang, Qingyu Lv, Yuling Zheng, Xuan Chen, Decong Kong, Wenhua Huang, Peng Liu, Hua Jiang, Yongqiang Jiang
Summary: A highly sensitive and accurate method using AlphaLISA was established for detecting T-2 toxin, with a detection range of 0.03-500 ng/mL, 90% recovery rate, and no cross-reaction with other mycotoxins.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Julie Meneely, Brett Greer, Oluwatobi Kolawole, Qiqi He, Christopher Elliott
Summary: The contamination of oat crops by trichothecene mycotoxins poses an ongoing threat to food safety. Effective testing methods and sampling strategies are crucial to limit human exposure. In this study, four commercial rapid diagnostic kits were evaluated, with the Neogen Reveal & REG; Q+ MAX for T-2/HT-2 Kit showing the most reliable, accurate, and cost-effective results.
Article
Food Science & Technology
Chris M. Maragos
Summary: T-2 toxin can be detected in wheat and maize using a portable mass spectrometer with APCI-MS. A rapid cleanup method was used to facilitate fast testing. The method can detect T-2 toxin in different types of wheat and maize above 0.2 mg/kg, but the sensitivity is not sufficient for the recommended levels by the European Commission, especially for HT-2 toxin.
Article
Chemistry, Analytical
Ratthaphol Charlermroj, Sudtida Phuengwas, Manlika Makornwattana, Thanasat Sooksimuang, Somboon Sahasithiwat, Waraporn Panchan, Wannee Sukbangnop, Christopher T. Elliott, Nitsara Karoonuthaisiri
Summary: This study combined the multiplexity of microarray technique and the simple and rapid characteristics of LFIA to enable simultaneous and quantitative detection of five mycotoxins. With the optimal conditions, the mu LFIA could accurately and quantitatively detect multiple mycotoxins at the same time. Combining the advantages of the novel reporter molecule and the multiplex capability of mu LFIA test, this system could simultaneously detect multiple mycotoxins in one sample with high specificity and sensitivity.
Review
Food Science & Technology
Shao-Ji Li, Guangzhi Zhang, Bin Xue, Qiaoling Ding, Lu Han, Jian-chu Huang, Fuhai Wu, Chonggao Li, Chunmin Yang
Summary: This review summarizes the updated knowledge on the toxicity of T-2 on poultry and potential strategies for detoxification of T-2 in poultry diet. The toxic effects of T-2 on poultry include cytotoxicity, immunotoxicity, hepatotoxicity, etc. Cytotoxicity is the primary toxicity, leading to pathological changes and impaired functions of multiple organs. Protective mechanisms and detoxifying agents can alleviate the toxic effects of T-2.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Food Science & Technology
Mengcong Li, Shuhui Liu, Lei Tan, Yan Luo, Zhangshan Gao, Jiwen Liu, Yuting Wu, Wentao Fan, Sarah DeSaeger, Suquan Song
Summary: This study found that FB1 contamination has a toxic effect on the intestine and disrupts its barrier function. FB1 also leads to excessive production of cytokines, further damaging the intestinal immune barrier. By inhibiting the expression of CerS2, FB1 induces endoplasmic reticulum stress, resulting in the destruction of the intestinal barrier. However, this damage can be restored by overexpression of CerS2 or the use of an ER stress inhibitor.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Food Science & Technology
N. N. Truong, K. Tesfamariam, L. Visintin, T. Goessens, S. De Saeger, C. Lachat, M. De Boevre
Summary: Mycotoxin contamination poses a global challenge to food safety and population health, leading to a range of adverse effects on human health. The accumulative effects of multiple mycotoxin exposures on health outcomes are not well understood. Various statistical approaches, such as generalised linear regressions in conjunction with regularisation methods and principal component analysis, have been used to assess mycotoxin exposure-health outcome associations, but they have certain limitations. Recently, alternative approaches such as weighted quantile sum regression and Bayesian kernel machine regression have been introduced to address the issues of high dimensionality and highly-correlated data in this context.
WORLD MYCOTOXIN JOURNAL
(2023)
Review
Chemistry, Analytical
Alexandra I. Arzhanukhina, Nadezhda S. Komova, Anton M. Pavlov, Alexey A. Serdobintsev, Tatiana Yu. Rusanova, Irina Yu. Goryacheva
Summary: Surface-enhanced Raman spectroscopy (SERS) is a powerful and up-to-date method in analytical chemistry, known for its high sensitivity and fingerprint recognition capabilities. In the medical field, SERS has been actively developed for label-free analysis of biologically important substances and potential on-site detection of toxins and food safety. Plasmonic nanostructures are essential for obtaining SERS signals, and electrospun nanofibers are a promising substrate due to their various advantages. This review summarizes the methods of incorporating plasmonic nanostructures into electrospun nanofiber substrates and provides detailed insights into the analytical applications of SERS-active electrospun nanofibers with embedded nanostructures for biologically significant molecules. Furthermore, the future prospects of these substrates in bioanalysis are discussed as the most promising area in analytical chemistry.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
P. A. Demina, O. A. Sindeeva, A. M. Abramova, M. S. Saveleva, G. B. Sukhorukov, I. Y. Goryacheva
Summary: This article investigates the performance of fluorescent photoconvertible polymer markers under different laser conditions and successfully achieves cell labeling. The authors describe the performance characteristics of fluorescent polymer capsules as an aqueous suspension and as a single capsule, and demonstrate the potential of these fluorescent markers for long-term live cell labeling.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Chemistry, Applied
Ye Tian, Mohamed F. Abdallah, Marthe De Boevre, Cheng Wang, Kris Audenaert, Sarah De Saeger, Aibo Wu
Summary: This study utilized an integrated workflow of mycotoxin analysis and non-targeted metabolomics to reveal the metabolic changes of Alternaria pathogens and the effects of Trichoderma strains on mycotoxin metabolism. The results showed that Trichoderma strains significantly influenced the whole metabolome and mycotoxin production of Alternaria pathogens, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alterations under various confrontation conditions.
Article
Food Science & Technology
Addisalem Mesfin, Carl Lachat, Seifu Hagos Gebreyesus, Meselech Roro, Kokeb Tesfamariam, Tefera Belachew, Marthe De Boevre, Sarah De Saeger
Summary: This study assessed the presence of multiple mycotoxins in breast milk and examined the relationship between total fumonisins and pre/post-harvest and dietary practices. The findings showed that the overall exposure to mycotoxins in breast milk was low, but fumonisins contamination was still present. No association was found between total fumonisins and pre/post-harvest and dietary practices.
Article
Pharmacology & Pharmacy
Orphelie Lootens, Marthe De Boevre, Jia Ning, Elke Gasthuys, Jan Van Bocxlaer, Sarah De Saeger, An Vermeulen
Summary: This article investigates the pharmacokinetic characteristics of aflatoxin B1 (AFB1) and finds that certain drugs can affect the metabolism of AFB1, while AFB1 has little impact on the metabolism of drugs.
Article
Agriculture, Dairy & Animal Science
Marie-Therese Koenig, Kai Froelich, Anabell Jandowsky, Tobias Knauf-Witzens, Christoph Langner, Richard Dietrich, Erwin Maertlbauer, Andrea Didier
Summary: Circular replicase-encoding single-stranded (CRESS) DNA viruses and other circular DNA agents were detected in bovids from African and Asian cattle, with the majority belonging to the BMMF groups 1 and 2, and a smaller part being CRESS viruses. This study provides new insights into the diversity and presence of circular DNA in ruminants used for food production worldwide.
Article
Chemistry, Analytical
Svetlana A. Mescheryakova, Ivan S. Matlakhov, Pavel D. Strokin, Daniil D. Drozd, Irina Yu. Goryacheva, Olga A. Goryacheva
Summary: Doxorubicin (DOX) is a commonly used anti-tumor drug in chemotherapy, but it has high cardio-, neuro- and cytotoxicity. Therefore, continuous monitoring of DOX concentrations in biofluids and tissues is important. This study demonstrates the capabilities of analytical nanosensors based on the quenching of fluorescence of alloyed CdZnSeS/ZnS quantum dots (QDs) for operative DOX detection. Optimized turn-off fluorescence nanosensors were developed for direct DOX determination in undiluted human plasma.
Article
Chemistry, Physical
Natalia E. Markina, Irina Yu. Goryacheva, Alexey V. Markin
Summary: The paper discusses the use of CD-modified AgNPs for the improvement of MTX determination through SERS. The CD-modified AgNPs increased the SERS signal of MTX in neutral media, simplifying the analysis and reducing the influence of endogenous components. The assay showed satisfactory precision, accuracy, and limit of detection for MTX determination in real human urine samples.
COLLOIDS AND INTERFACES
(2023)
Article
Nutrition & Dietetics
Yuri Bastos-Moreira, Lionel Ouedraogo, Marthe De Boevre, Alemayehu Argaw, Brenda de Kok, Giles T. Hanley-Cook, Lishi Deng, Moctar Ouedraogo, Anderson Compaore, Kokeb Tesfamariam, Rasmane Ganaba, Lieven Huybregts, Laeticia Celine Toe, Carl Lachat, Patrick Kolsteren, Sarah De Saeger, Trenton Dailey-Chwalibog
Summary: This study aims to evaluate the physiological effect of multi-micronutrient-fortified BEP supplementation on pregnant and lactating women and their infants. Biological specimens will be collected and multi-omics profiles and biomonitoring markers will be used to assess the effects of BEP supplementation on maternal health, birth outcomes, and infant growth.
Review
Food Science & Technology
Xiangrong Chen, Mohamed F. Abdallah, Sofie Landschoot, Kris Audenaert, Sarah De Saeger, Xiangfeng Chen, Andreja Rajkovic
Summary: This review provides an updated overview of the (co-)occurrence and (co-)contamination of A. flavus and F. verticillioides and their mycotoxins in maize. The gathered data predict an increase in their occurrence and virulence worldwide, especially in European cold climate countries. While antagonistic interactions were observed between these fungi, the co-contamination of AFB1 and FB1 has significantly risen in Europe.
Review
Food Science & Technology
Octavian Augustin Mihalache, Marthe De Boevre, Luca Dellafiora, Sarah De Saeger, Antonio Moretti, Laetitia Pinson-Gadais, Nadia Ponts, Florence Richard-Forget, Antonia Susca, Chiara Dall'Asta
Summary: This systematic review aims to update the knowledge on the occurrence and co-occurrence of non-regulated mycotoxins, while also providing an overview of current regulations. The study found 15 non-regulated mycotoxins in 19 food categories worldwide, with 38 different combinations of these mycotoxins. The authors emphasize the importance of continuous monitoring, scientific exchange, and generation of high-quality data to effectively address concerns related to non-regulated mycotoxin toxicity. Additionally, obtaining more data concerning the co-occurrence of regulated and non-regulated mycotoxins could support multiple chemical risk assessment methodologies.
