期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 393, 期 6-7, 页码 1601-1605出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2588-5
关键词
Biosensors; Self-assembled monolayers; Electrophysiology
资金
- European Commission [NMP4CT-2005-017114]
- UK Interdisciplinary Research Centre in Bio-Nanotechnology [R45659/01]
We describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid-liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid-alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据