期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 393, 期 6-7, 页码 1563-1570出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2595-6
关键词
Protein immobilization; Microstructure; Nanostructure; Patterning; Nitrilotriacetic acid; Biotinylation
Protein immobilization into micro and nano-scaled patterns opens exciting possibilities in fundamental and applied research. Developing efficient capturing techniques while preserving the structural and functional integrity of the proteins on surfaces is a key challenge for surface scientists. In this paper, current techniques for site-specific protein immobilization into engineered surface architectures are reviewed. Fundamental principles for functional protein immobilization on solid supports are discussed and popular affinity-based recognition pairs and their application for capturing proteins into nano and microstructures are presented.
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