Article
Chemistry, Analytical
Bryan Gosselin, Maurice Retout, Raphael Dutour, Ludovic Troian-Gautier, Robin Bevernaegie, Sophie Herens, Philippe Lefevre, Olivier Denis, Gilles Bruylants, Ivan Jabin
Summary: A dipstick assay using silver nanoparticles stabilized by a thin calix[4]arene-based coating was developed for detecting Anti-SARS-CoV-2 IgG in clinical samples. Compared to traditional lateral-flow immunoassays using gold nanoparticles, this silver nanoparticle-based system showed a lower limit of detection and similar signals with a smaller number of particles. The dipstick assay demonstrated impressive results in real clinical samples, with 100% specificity for negative samples and a sensitivity of 73% for positive samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jeonghyo Kim, Van Tan Tran, Sangjin Oh, Minji Jang, Dong Kun Lee, Jong Chul Hong, Tae Jung Park, Hwa-Jung Kim, Jaebeom Lee
Summary: The MagPlas NZs, combining magnetic enrichment and catalytic amplification, offer a streamlined, high-throughput operation with ultrahigh sensitivity in urine-based tuberculosis diagnosis and anti-TB therapy monitoring. The MagPlas ELISA method showed fast (<3 h) and highly sensitive (up to pM) detection of urinary TB antigen, with robust sensitivity and high specificity in clinical studies. This methodology promises noninvasive therapeutic response monitoring and has potential for use in point-of-care testing for TB control and other acute infections.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yingying Zhong, Xian Wang, Ruyan Zha, Chen Wang, Huijuan Zhang, Yanying Wang, Chunya Li
Summary: The study utilized a novel aptasensor to achieve simultaneous detection of CEA and CA153 tumor markers, demonstrating excellent sensitivity and accuracy. Experimental results showed a high consistency between the aptasensor's detection of CEA and CA153 in serum samples and the traditional ELISA method, indicating promising clinical application prospects.
Article
Food Science & Technology
Yao Yu, Yansong Li, Qi Zhang, Yonghong Zha, Shiying Lu, Yong Yang, Peiwu Li, Yu Zhou
Summary: A colorimetric immunoassay based on the aggregation of gold nanoparticles was proposed for the detection of FB1, with a low detection limit, no cross-reaction with other mycotoxins, and potential application for food safety monitoring.
Article
Chemistry, Multidisciplinary
Liran Tian, Xiangwei Song, Tianjiao Liu, Anfeng Li, Yang Ning, Xiuyi Hua, Deming Dong, Dapeng Liang
Summary: A UV-Visible and fluorescence detection method was designed for the determination of acetamiprid based on the specific identification ability of aptamers. The presence of acetamiprid could be clearly determined through the aggregation of gold nanoparticles and the redshifting of the UV-vis absorption peak. This combined method showed a wide detection range and a low detection limit, making it a potential simple method for the determination of acetamiprid residues in natural water bodies.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fan Li, Tianshuang He, Sihao Wu, Zoujun Peng, Ping Qiu, Xiaomin Tang
Summary: The study developed a novel uric acid detection method using chitosan stabilized gold nanoparticles, which showed high sensitivity and simplicity. The method was successfully applied to human serum and urine samples with high recovery rates, indicating its potential as an alternative for clinical medicine and daily monitoring.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Zoe Bradley, Patrick A. Coleman, Melissa A. Courtney, Sam Fishlock, Joseph McGrath, Therese Uniacke-Lowe, Nikhil Bhalla, James A. McLaughlin, John Hogan, John P. Hanrahan, Ke-Ting Yan, Philip McKee
Summary: Sepsis is the body's response to an infection. Current diagnostic testing equipment is not available in primary care settings and has long waiting times. Lateral flow devices (LFDs) could be used in point-of-care (POC) settings for sepsis detection, but they lack the required sensitivity.
Article
Chemistry, Multidisciplinary
Abdulrahman A. Almehizia, Ahmed M. Naglah, Lamees S. Alrasheed, Mashael G. Alanazi, Abdel El-Galil E. Amr, Ayman H. Kamel
Summary: A robust, reliable, and affordable paper-based analytical apparatus for potentiometric sensing has been developed for the rapid identification of cardiovascular disorders, which is particularly important for point-of-care monitoring.
Article
Chemistry, Analytical
Nikan Momenbeitollahi, Joshua van der Zalm, Aicheng Chen, Huiyan Li
Summary: Quantifying proteins is important for understanding human health and disease, and sensitive protein quantification techniques are required for disease diagnosis. This study presents a novel signal enhancement method for fluorescence-based protein detection using gold nanoparticles on a nitrocellulose membrane. It achieved metal enhanced fluorescence and improved the sensitivity of protein quantification.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Thithawat Trakoolwilaiwan, Yasuhiro Takeuchi, Terence S. Leung, Matej Sebek, Liudmyla Storozhuk, Linh Nguyen, Le Duc Tung, Nguyen Thi Kim Thanh
Summary: Dengue disease is a viral infection that has been widespread in tropical regions. Researchers developed a colorimetric thermal sensing LFA format for the detection of dengue virus NS1, which has the potential to enhance detection sensitivity and provide a visible signal without the need for an infrared camera.
Article
Materials Science, Biomaterials
Dong Zhao, Pengli Xiao, Xiushan Dong, Yang Ge, Xing Guo, Jianlong Ji, Yongqiang Cheng, Shengbo Sang
Summary: Recently, a mechanical biosensor based on membrane-mediated magneto-stress-electric coupled sensitization (MSEC-MMB) was developed to improve the sensitivity and practicality of single molecule detection in clinical applications. The MSEC-MMB showed enhanced sensitivity by introducing Fe3O4 nanoparticles and a magnetic field to the traditional stress-electric biosensors, resulting in a LOD of 24 pg mL-1 for HSA. The MSEC-MMB also demonstrated high specificity, selectivity, and stability, making it a promising platform for early nephropathy diagnosis.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Yun Zhao, Sijie Huang, Mengjia Chao, Yulong Wang, Pengyan Liu, Pan Li, Xuechen Fang, Michael N. Routledge, Chifang Peng, Cunzheng Zhang
Summary: A monoclonal antibody against SBT was prepared using a designed hapten, and polydopamine coated gold nanoparticles (PDA/AuNPs) with controlled shell thickness and packing density were synthesized, which improved the sensitivity of the immunochromatographic assay strip.
Article
Chemistry, Applied
Yuanshang Wang, Pengfei Du, Am Abd El-Aty, Ge Chen, Huiyan Jia, Xueyan Cui, Emel Oz, Yudan Zhang, Xiuyuan Zhang, Guoxin Qin, Feiyan Yan, Jing Wang, Maojun Jin, Bruce D. Hammock
Summary: A novel visual method for detecting triazophos using competitive bio-barcode immunoassay was established, with a detection range between 0.05 and 10 ng/mL. The method showed high percent recovery and stability, offering a new insight for rapid detection of small molecule pesticides in various matrices.
Article
Chemistry, Analytical
Kyeongnyeon Kim, Do Kyoung Han, Namhyun Choi, Soo Hyeon Kim, Younju Joung, Kihyun Kim, Ngoc Thanh Ho, Sang-Woo Joo, Jaebum Choo
Summary: This study developed a new concept of using a dual-flow SERS-based LFA sensor to improve detection sensitivity by flowing two different sizes of gold nanoparticle solutions. The sensor successfully detected thyroid-stimulating hormone less than 0.5 mu IU/mL, which cannot be measured with a SERS-based single-flow LFA sensor.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Pornsuda Maraming, Nang Noon Shean Aye, Patcharee Boonsiri, Sakda Daduang, Onanong Buhome, Jureerut Daduang
Summary: Polydopamine nanoparticles (PDA-NPs)-functionalized electrochemical aptasensor was developed for the rapid, sensitive, and cost-effective detection of glycated albumin (GA). The aptasensor showed a broad linearity for GA detection in a clinically relevant range, with low detection limit, good reproducibility, and practicality. Furthermore, the aptasensor demonstrated high selectivity towards GA and potential clinical application in serum samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Analytical
Donato Calabria, Maria Maddalena Calabretta, Martina Zangheri, Elisa Marchegiani, Ilaria Trozzi, Massimo Guardigli, Elisa Michelini, Fabio Di Nardo, Laura Anfossi, Claudio Baggiani, Mara Mirasoli
Summary: Paper-based lateral-flow immunoassays have achieved commercial success and are continuously growing in the field of diagnostics. These assays, which provide qualitative information about the target analyte, have the potential to be adapted for ultrasensitive quantitative analysis.
Article
Chemistry, Analytical
Martina Zangheri, Fabio Di Nardo, Donato Calabria, Elisa Marchegiani, Laura Anfossi, Massimo Guardigli, Mara Mirasoli, Claudio Baggiani, Aldo Roda
Summary: Exposure to mycotoxins poses a serious health risk, with OTA being one of the most toxic types. LFIA-based devices are proposed for screening OTA contamination, and a smartphone-based biosensor has been developed for OTA detection in wine and instant coffee samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Maryam Mousivand, Kowsar Bagherzadeh, Laura Anfossi, Mohammad Javan-Nikkhah
Summary: The study used computational techniques to improve the efficiency of screening high affinity aptamers for mycotoxins and conducted experimental affinity measurements. Research findings showed that aptamer F20 exhibited the best free binding energy and complex stability, primarily binding to AFB1 through precise shape complementarity and dynamic water intermediate interactions, with van der Waals contacts identified as the dominant energy component in all complexes.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Analytical
Simone Cavalera, Giulia Pezzoni, Santina Grazioli, Emiliana Brocchi, Stefano Baselli, Davide Lelli, Barbara Colitti, Thea Serra, Fabio Di Nardo, Matteo Chiarello, Valentina Testa, Sergio Rosati, Claudio Baggiani, Laura Anfossi
Summary: A highly sensitive colorimetric LFIA for the point-of-need diagnosis of LSDV was developed using two monoclonal antibodies and gold nanoparticles. The optimized LFIA showed a low limit of detection, high repeatability, and specificity, making it a useful tool for LSD diagnosis in resource-limited settings.
Article
Polymer Science
Valentina Testa, Laura Anfossi, Simone Cavalera, Matteo Chiarello, Fabio Di Nardo, Thea Serra, Claudio Baggiani
Summary: Increasing amounts of surfactant decrease the binding affinity for ionic ligands strongly, and more weakly for non-ionic ligands. This effect is general, occurring for both 2,4,5-T and 2,4-D, as well as for imprinted and non-imprinted polymers, mainly depending on the presence or absence of an ionic charge.
Article
Food Science & Technology
Cristina Capodicasa, Erica Bastiani, Thea Serra, Laura Anfossi, Marcello Catellani
Summary: A new green competitive ELISA method for the quantification of aflatoxin M1 in raw milk, based on plant-produced recombinant monoclonal antibodies, has been successfully developed. The method showed high accuracy within the decision levels required by EU legislation, with a detection limit of 3 ng/L and a quantification range of 5-110 ng/L.
Review
Chemistry, Analytical
Fabio Di Nardo, Laura Anfossi, Claudio Baggiani
Summary: This article discusses the application of molecularly imprinted polymers in immunoassays, highlighting the key issues that have hindered their widespread use and suggesting strategies to overcome them.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Maryam Mousivand, Mohammad Javan-Nikkhah, Laura Anfossi, Fabio Di Nardo, Matteo Salina, Kowsar Bagherzadeh
Summary: Due to technical challenges, computational simulation studies are effective tools for designing small binding aptamers. In this study, two novel aptamers were successfully used in lateral flow aptasensors and reflective phantom interface (RPI) platform for the detection of aflatoxin B1. The developed aptasensors showed high sensitivity and selectivity for AFB1, and successfully detected it in maize flour.
Article
Chemistry, Analytical
Simone Cavalera, Thea Serra, Antonio Abad-Fuentes, Josep V. Mercader, Antonio Abad-Somovilla, Fabio Di Nardo, Antonio D'Avolio, Amedeo De Nicolo, Valentina Testa, Matteo Chiarello, Claudio Baggiani, Laura Anfossi
Summary: Highly active antiretroviral therapy (HAART) can have toxic effects due to potent drugs like Tenofovir (TFV). Successful treatment relies on proper management of TFV, and therapeutic drug monitoring (TDM) of TFV concentrations in saliva can be a valuable tool for determining treatment compliance. We have developed a highly sensitive ELISA and LFIA for quantification and screening of TFV in saliva, respectively.
Article
Materials Science, Biomaterials
Matteo Chiarello, Laura Anfossi, Simone Cavalera, Fabio Di Nardo, Thea Serra, Fabrizio Sordello, Claudio Baggiani
Summary: Solid phase synthesis (SPS) of molecularly imprinted nanopolymers (nanoMIPs) is an innovative method for preparing nanomaterials with tailor-made molecular recognition properties for peptides and proteins. This study investigated the effect of different cross-linkers on the binding properties and selectivity of protein-imprinted nanoMIPs prepared by SPS, and found that the structure of the cross-linker significantly influenced the binding properties and selectivity of the nanoMIPs.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemical Research Methods
Mercedes Vazquez, Laura Anfossi, Hadar Ben-Yoav, Lorena Dieguez, Thomas Karopka, Bartolomeo Della Ventura, Sara Abalde-Cela, Antonio Minopoli, Fabio Di Nardo, Vikas Kumar Shukla, Alexandra Teixeira, Asta Tvarijonaviciute, Lorena Franco-Martinez
Summary: In recent years, a variety of free or economical clinical pathology technologies have been developed globally, such as 3D printing and Arduino approaches that significantly reduce costs. Point-of-care-tests (POCT) can also minimize the need for specific instruments or personnel, making clinical pathology more accessible. Additionally, the field of health informatics offers numerous free digital tools for use.
