Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Review
Infectious Diseases
Eun-Jeong Yoon, Seok Hoon Jeong
Summary: MALDI-TOF MS has become a routine diagnostic process for infectious diseases, offering a fast, low-cost, and simple methodology in clinical microbiology laboratories. It can identify antimicrobial resistance through various approaches, such as comparing bacterial spectra and determining peak shifts in the presence of antimicrobial drugs. This technique shows promise for accurate and rapid identification of AMR in bacteria.
Article
Food Science & Technology
Jana Vyrostkova, Ivana Regecova, Eva Dudrikova, Slavomir Marcincak, Maria Vargova, Mariana Kovacova, Jana Mal'ova
Summary: This study calculated the antibiotic resistance profiles of Enterococcus faecium, E. faecalis, and E. durans isolated from traditional sheep and goat cheeses in a border area of Slovakia with Hungary. E. faecalis showed higher resistance compared to E. durans and E. faecium, with high resistance to rifampicin and vancomycin. E. durans and E. faecium also exhibited resistance, with a high percentage of multidrug-resistant strains.
Article
Polymer Science
Deepak A. Yaraguppi, Zabin K. Bagewadi, Nilkamal Mahanta, Surya P. Singh, T. M. Yunus Khan, Sanjay H. Deshpande, Chaitra Soratur, Simita Das, Dimple Saikia
Summary: In this study, the lipopeptide iturin A was synthesized from Bacillus aryabhattai using gene engineering techniques, and it was found to have promising biosurfactant properties and the ability to enhance oil recovery in oil fields.
Article
Microbiology
Ashwini Kamble, Shilpa Naik, Manju Talathi, Deepali Jadhav, Samradni Pingale, Ruchika Kaul-Ghanekar
Summary: The study aimed to isolate and identify potential probiotics from vaginal swabs of healthy women and evaluate their activity against vaginal pathogens isolated from cervical cancer patients. The results showed that certain lactic acid bacteria strains exhibited significant antimicrobial activity against cervical cancer-associated vaginal pathogens, indicating their potential as biotherapeutics.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Polymer Science
Ibrahim A. Alhagri, Talal F. Qahtan, Mohammed O. Farea, Ahmed N. Al-Hakimi, Sadeq M. Al-Hazmy, Saeed El-Sayed Saeed, Abuzar E. A. E. Albadri
Summary: This article examines the synthesis, optical, and electrical properties of composites composed of polyethylene oxide (PEO), carboxymethyl cellulose (CMC), and titanium dioxide (TiO2) nanoparticles. Flexible nanocomposite samples of PEO, CMC, and TiO2 nanoparticles were swiftly produced using the cast synthesis method. XRD and FT-IR analysis were conducted to analyze the sample structures. The study demonstrates the effectiveness of the PEO/CMC blend in interacting with TiO2 nanoparticles. The optical properties, such as the energy band gap, were studied using UV/Vis optical absorbance, showing that the energy gap narrows as the TiO2 nanoparticle weight fraction increases. TEM and SEM images reveal the size, shape, and distribution of the TiO2 nanoparticles. The enhanced antibacterial activity in the nanocomposites suggests their potential use in electrochemical devices.
Article
Immunology
Tzu-Ping Weng, Ching-Lung Lo, Wen-Liang Lin, Jen-Chieh Lee, Ming -Chi Li, Wen-Chien Ko, Nan-Yao Lee
Summary: This study aimed to evaluate the impact of integrating antimicrobial stewardship intervention (ASI) with rapid organism identification on antimicrobial therapy-related outcomes in patients with bloodstream infections (BSIs). The results showed that the combination of ASI and MALDI-TOF-MS could significantly shorten the time to organism identification and reduce the length of hospital stay for BSI patients, leading to a decrease in 30-day mortality.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Review
Immunology
Ayman Elbehiry, Musaad Aldubaib, Adil Abalkhail, Eman Marzouk, Ahmad ALbeloushi, Ihab Moussa, Mai Ibrahem, Hamad Albazie, Abdullah Alqarni, Sulaiman Anagreyyah, Saleh Alghamdi, Mohammed Rawway
Summary: Healthcare settings have been utilizing MALDI-TOF MS since 2010, which offers advantages such as ease of use, speed, accuracy, and low cost. It is particularly useful for identifying highly virulent and drug-resistant diseases. However, distinguishing between similar species can be challenging, but additional testing can usually correct this issue.
Article
Food Science & Technology
Hongyan Yang, Lan Wang, Fei Pan, Libo Yuan, Heng Du, Pei Zhang, Kui Lu
Summary: In this study, a self-assembling lipopeptide C12-CL5 was developed by modifying the structure of the antimicrobial peptide CL5. The C12-CL5 showed potent activity against both Gram-negative and Gram-positive bacteria, with good thermal stability, excellent salt tolerance, and strong and rapid killing kinetics. The study also revealed the antimicrobial mechanism of C12-CL5, providing insights and basis for the development of potent and broad-spectrum antimicrobial peptides in the food industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Shuvo Singha, Gerrit Koop, Ylva Persson, Delower Hossain, Lauren Scanlon, Marjolein Derks, Md Ahasanul Hoque, Md Mizanur Rahman
Summary: The incidence of clinical mastitis is high in commercial dairy farms in Bangladesh, with Streptococci and non-aureus Staphylococci being the most commonly isolated bacteria. These bacteria often show resistance against penicillin and oxacillin, highlighting the need for improved udder health management and prudent use of antimicrobial agents.
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Bruno Kotska Rodino-Janeiro, Paula Guijarro-Sanchez, Tyler Alioto, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, German Bou, Marina Oviano
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for direct detection of KPC-producing isolates by analyzing the 11,109 Da mass peak representing the P019 protein. The study found the P019 peak prevalence in bla(KPC) K. pneumoniae isolates to be 49.2%, while it was absent in other carbapenemase or noncarbapenemase producers. Variability of P019 presence was observed in different geographical locations within the EuSCAPE collection, indicating a need for reconsideration when applying this technique in a clinical setting.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Xinghong Zhao, Xiaoqi Wang, Rhythm Shukla, Raj Kumar, Markus Weingarth, Eefjan Breukink, Oscar P. Kuipers
Summary: The study identified and characterized a novel antimicrobial candidate, brevibacillin 2V, from Brevibacillus laterosporus DSM 25, which exhibited strong antimicrobial activity against Gram-positive bacterial pathogens and low cytotoxicity to eukaryotic cells compared to other NRPs of the same family. Brevibacillin 2V showed good stability in human plasma and has the potential to be developed as a promising antibiotic with low hemolytic activity and cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Pathology
Gloria Viboud, Holly Asaro, Michael Bailou Huang
Summary: MALDI-TOF shows promise as a tool for AMR detection, but further research, development, and validation efforts are needed for its wider clinical adoption.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jana Vyrostkova, Ivana Regecova, Frantisek Zigo, Boris Semjon, Gabriela Gregova
Summary: Limited studies have been conducted on antimicrobial resistance in certain species of staphylococci isolated from unpasteurized milk products. This study aimed to investigate the occurrence and antimicrobial resistance of specific staphylococci species in sheep and goat cheeses made from unpasteurized milk. The results showed high resistance to penicillin and erythromycin in all tested species.
Article
Agriculture, Dairy & Animal Science
Leszek Guz, Aneta Nowakiewicz, Krzysztof Puk, Przemyslaw Zieba, Sebastian Gnat, Lukasz Matuszewski
Summary: The study identified a high prevalence of Aeromonas species in water sources and European pond turtles from Eastern Poland, with most isolates showing resistance to multiple antibiotics and carrying multiple virulence genes.
Article
Materials Science, Textiles
Ajaya Kumar Behera, Ramkrishna Sen, Basudam Adhikari
Summary: The aim of this investigation was to develop composites with high mechanical strength and complete biodegradation property. Jute-modified soy composites were developed using mild alkali-treated jute and poly(vinyl alcohol)-modified nanoclay-based soy resins. The tensile strength of the composite was enhanced by 75% and impact strength by 30% compared to normal jute-soy composites. The biodegradation study showed that these composites can be used as a replacement for nonbiodegradable plastics in various sectors.
JOURNAL OF NATURAL FIBERS
(2022)
Article
Engineering, Chemical
Geetanjali Yadav, Leonard A. Fabiano, Lindsay Soh, Julie Zimmerman, Ramkrishna Sen, Warren D. Seider
Summary: The study introduces a new method using carbon dioxide to improve algae-to-biodiesel processes, focusing on reducing costs, improving biodiesel generation efficiency, and increasing the utilization of proteins and Ω-3 fatty acids. This approach enhances the transesterification reaction rate and overall efficiency, leading to a significant reduction in the modeled price of biodiesel.
Article
Materials Science, Biomaterials
Sayanti Datta, Arun Prabhu Rameshbabu, Kamakshi Bankoti, Madhurima Roy, Chandrika Gupta, Subhodeep Jana, Amit Kumar Das, Ramkrishna Sen, Santanu Dhara
Summary: The biohybrid hydrogel DBM/OC based on decellularized bone ECM and fatty acid modified chitosan showed excellent mechanical strength and antimicrobial potential, supporting proliferation and differentiation of stem cells for bone regeneration. Its efficacy as a functional cell-delivery vehicle and osteoinductive template was demonstrated through in vivo and ex-vivo studies.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Environmental Sciences
Ipsita Dipamitra Behera, Manoranjan Nayak, Subrata Biswas, Bhim Charan Meikap, Ramkrishna Sen
Summary: In this study, a two-step bioaugmentation strategy (TSBS) using indigenous bacterial consortium showed enhanced degradation of total petroleum hydrocarbons (TPH) from petroleum refinery sludge (PRS). The TSBS resulted in a higher TPH degradation rate compared to a single-step bioaugmentation strategy (SSBS) within a 15-day incubation period, demonstrating its effectiveness in TPH removal.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Biotechnology & Applied Microbiology
Rabiya Rabiya, Ramkrishna Sen
Summary: In this study, an AI-driven h-SVM optimization strategy was used to improve the production of EPS by Bacillus licheniformis. The results showed that SVM performed better than RSM in data modeling, and when combined with TLBO and PSO optimization algorithms, a maximum EPS concentration of 64±1.1 g/L was obtained, which is about a 5-fold increase compared to the un-optimized medium.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Goldy De Bhowmick, Rowena M. Briones, Soren Thiele-Bruhn, Ramkrishna Sen, Ajit K. Sarmah
Summary: The study found that activated macroalgae biochar (MACAC) and microalgae biochar (MICAC) exhibited superior adsorption performance for metformin hydrochloride (MET), with maximum adsorption occurring at a pH of 7 and up to 76% removal of MET. Activation strengthened chemisorption, leading to the formation of oxygen-containing surface functional groups, and the lowest zeta potential observed at pH 7 enhanced the electrostatic force of attraction for both biochars.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Ipsita Dipamitra Behera, Manoranjan Nayak, Asmita Mishra, Bhim Charan Meikap, Ramkrishna Sen
Summary: The release of petroleum hydrocarbon pollutants is a serious problem for both terrestrial and marine ecosystems. This study compared different biodegradation strategies for mitigating total petroleum hydrocarbon (TPH) in petroleum refinery sludge and found that the combined action of bioaugmentation and biostimulation, specifically the use of an indigenously developed bacterial consortium and poultry litter extract (BABSS), resulted in a 90.3 +/- 3.7% reduction in TPH within 21 days. The efficacy of the strategies was confirmed by GC-FID analysis.
MARINE POLLUTION BULLETIN
(2022)
Article
Materials Science, Biomaterials
Debajyoti Palai, Trina Roy, P. Siva Prasad, Chinmay Hazra, Santanu Dhara, Ramkrishna Sen, Siddhartha Das, Karabi Das
Summary: Three Zn-based alloys with different Cu contents were developed and showed improved mechanical properties. The degradation behavior and cytocompatibility of the alloys varied, with Zn3Cu exhibiting the highest strength and cytocompatibility.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Sayak Mitra, Riddhiman Dhar, Ramkrishna Sen
Summary: Non-ribosomal peptides (NRPs) are secondary metabolites with high commercial importance. However, the low synthesis yield by bacteria and losses during purification hinder their industrial-scale production. This review discusses strategies for enhancing NRP yield, including isolation of efficient producer strains, optimization of bioprocesses, and the use of metabolic engineering and synthetic biology techniques to redirect metabolic network fluxes. The review also provides insights into the regulation of NRP biosynthesis and highlights the potential of computational techniques in designing cellular factories for NRP production.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Green & Sustainable Science & Technology
Jayita Chopra, Vivek Rangarajan, Ramkrishna Sen
Summary: This review discusses the research advancements and optimization strategies for sustainable biodiesel production from oleaginous yeasts. It highlights the potential for producing multiple products and suggests a bio-refinery model for value-added production.
SUSTAINABLE ENERGY TECHNOLOGIES AND ASSESSMENTS
(2022)
Article
Nanoscience & Nanotechnology
Antara Sikder, Saugat Mondal, Rabiya Rabiya, Pritha Biswas, Subham Pal, Ramkrishna Sen, N. D. Pradeep Singh
Summary: This study demonstrates the design and synthesis of a novel single-component prodrug for the cocktail release of therapeutics, enabling spatiotemporal control over bacterial growth. This concept can be applied to develop other advanced antibacterial biomaterials and find wide applications in antibacterial research.
ACS APPLIED NANO MATERIALS
(2022)
Article
Green & Sustainable Science & Technology
Romit Mitra, Anirban Das Gupta, Ravi Ranjan Kumar, Ramkrishna Sen
Summary: This study demonstrates that microalgae can utilize sodium bicarbonate as an inorganic carbon source to enhance carbon dioxide fixation rate and algal biomass production. By employing a smart intermittent bicarbonate feeding strategy, biomass concentration can be further improved. Additionally, bicarbonate promotes self-flocculation and the formation of extracellular polymeric substances, leading to enhanced nutrient uptake and gas exchange, thus improving biomass production and self-settling. This innovative approach can be scaled up to efficiently sequester flue-gas CO2 released by thermal power plants and other fossil fuel-based industries through algal cultivation.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Engineering, Environmental
Debanjali Dey, Shamik Chowdhury, Ramkrishna Sen
Summary: Antibiotic resistance is a major global crisis, with antibiotic resistant bacteria and genes detected as emerging biological contaminants. The release of antibiotic residue from human waste and pharmaceutical effluents into wastewater leads to the proliferation of resistance genes and bacteria in natural environments. Nanotechnology and nanomaterials offer potential solutions for combating antibiotic resistance, with ongoing efforts to develop efficient nanotechnology-based treatment strategies. However, the long-term efficacy of nano-based treatment under realistic conditions remains a challenge for commercialization. This review highlights the potential of nanotechnology in removing antibiotic resistance genes and bacteria, and discusses advanced detection technologies in use or being developed beyond the laboratory scale.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Chemistry, Physical
P. Siva Prasad, Chinmay Hazra, Sambedan Jena, Prasanna Kumar Byram, Ramkrishna Sen, Nishant Chakravorty, Siddhartha Das, Karabi Das
Summary: A microbial green surfactant was used to facilitate the deposition of brushite and hydroxyapatite coatings on stainless steel. The addition of the surfactant affected the composition and morphology of the coatings. The surfactant inhibited the growth of hydroxyapatite and resulted in well-defined flower-like coatings. The addition of the surfactant also improved the corrosion resistance, degradation, antibacterial properties, and hemocompatibility of the coatings.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Materials Science, Biomaterials
Sayanti Datta, Arun Prabhu Rameshbabu, Kamakshi Bankoti, Subhodeep Jana, Sabyasachi Roy, Ramkrishna Sen, Santanu Dhara
Summary: The study developed a multifunctional hydrogel construct as an osteoinductive and osteoconductive platform for stimulating bone tissue regeneration. The experiment demonstrated the potential of the hydrogel construct for controlled delivery of ALN and BMP-2, leading to enhanced bone regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
