期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 393, 期 4, 页码 1157-1163出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-008-2448-3
关键词
Surface plasmon resonance imaging; Protein array; Cancer biomarkers
资金
- National Science Foundation [CBET-0528605]
In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.
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