Article
Environmental Sciences
Kleopatra Miserli, Christina Kosma, Ioannis Konstantinou
Summary: Pharmaceuticals released from wastewater treatment plants are emerging contaminants that can have biological effects at low concentrations. Conventional wastewater treatment methods are unable to completely remove these contaminants, leading to their presence in sewage sludge. This study developed a method to detect pharmaceuticals in sewage sludge and hydrochar using a combination of extraction and chromatography techniques. The method was successful in detecting various pharmaceutical compounds in the samples tested.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Adam Tolgyesi, Gerda Korozs, Edgar Toth, Maria Balint, Xingmao Ma, Virender K. Sharma
Summary: A new method using LC-MS/MS for quantifying phenoxy herbicides in water samples was presented. The method utilized an automated SPE process with hydrophilic modified polystyrene and divinylbenzene cartridges at low pH, reducing organic solvent consumption and providing fully automated sample pre-concentration. Stable isotopically labelled analogues were used as internal standards to ensure accurate measurement. Analysis of thirty-two actual water samples showed a 40% detection rate of herbicides with concentrations comparable to other regions.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Fei Yang, Haozhe Cui, Chunqiong Wang, Ying Wang, Wenjing Zhu, Huimin Deng, Shanshan Liu, Zhaoyang Bian, Junli Lu, Gangling Tang, Yuan Ji
Summary: This study utilized reversed-phase liquid chromatography-tandem mass spectrometry and supercritical fluid chromatography-tandem mass spectrometry to determine the stereoisomers of chlorfenvinphos and dimethylvinphos in tobacco. A modified quick, easy, cheap, effective, rugged, and safe technique using spherical carbon was employed for the extraction and purification of tobacco samples. Both methods were compared comprehensively in terms of methods validation parameters, showing excellent linearity and adequate recoveries for the targeted stereoisomers.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Weili Xu, Jilong Li, Ji Feng, Zhibing Wang, Hanqi Zhang
Summary: A novel in-syringe temperature-controlled liquid-liquid microextraction based on solidified floating ionic liquid (in-syringe TC-LLME-SFIL) combined with high performance liquid chromatography was developed for the simultaneous determination of herbicides in commercial vegetable protein drinks. The method utilized an ionic liquid as the extraction solvent, and after deproteinization and solid phase extraction column clean up, fine droplets were formed with heating and vortexing, followed by analysis using HPLC, achieving good linearities, low detection limits, and high spiked recoveries.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Julien Sechet, Camille Roux, Anne Plessis, Delphine Effroy, Anne Frey, Francois Perreau, Catherine Biniek, Anja Krieger-Liszkay, David Macherel, Helen M. North, Hakim Mireau, Annie Marion-Poll
Article
Multidisciplinary Sciences
Ryan L. Leverenz, Markus Sutter, Adjele Wilson, Sayan Gupta, Adrien Thurotte, Celine Bourcier de Carbon, Christopher J. Petzold, Corie Ralston, Francois Perreau, Diana Kirilovsky, Cheryl A. Kerfeld
Article
Multidisciplinary Sciences
Celine Bourcier de Carbon, Adrien Thurotte, Adjele Wilson, Francois Perreau, Diana Kirilovsky
SCIENTIFIC REPORTS
(2015)
Article
Biochemistry & Molecular Biology
Matthew R. Melnicki, Ryan L. Leverenz, Markus Sutter, Rocio Lopez-Igual, Adjele Wilson, Emily G. Pawlowski, Francois Perreau, Diana Kirilovsky, Cheryl A. Kerfeld
Article
Plant Sciences
Kathleen Feilke, Peter Streb, Gabriel Cornic, Francois Perreau, Jerzy Kruk, Anja Krieger-Liszkay
Article
Plant Sciences
Rocio Lopez-Igual, Adjele Wilson, Ryan L. Leverenz, Matthew R. Melnicki, Celine Bourcier de Carbon, Markus Sutter, Aiko Turmo, Francois Perreau, Cheryl A. Kerfeld, Diana Kirilovsky
Article
Plant Sciences
Julien Sechet, Anne Frey, Delphine Effroy-Cuzzi, Adeline Berger, Francois Perreau, Gwendal Cueff, Delphine Charif, Loic Rajjou, Gregory Mouille, Helen M. North, Annie Marion-Poll
Article
Plant Sciences
Marina Pfalz, Maisara Mukhaimar, Franois Perreau, Jayne Kirk, Cecilie Ida Cetti Hansen, Carl Erik Olsen, Niels Agerbirk, Juergen Kroymann
Article
Biochemical Research Methods
Stephanie Boutet-Mercey, Francois Perreau, Amelie Roux, Guillaume Clave, Jean-Paul Pillot, Isabelle Schmitz-Afonso, David Touboul, Gregory Mouille, Catherine Rameau, Francois-Didier Boyer
PHYTOCHEMICAL ANALYSIS
(2018)
Article
Plant Sciences
Fernando Muzzopappa, Adjele Wilson, Vinosa Yogarajah, Sandrine Cot, Francois Perreau, Cedric Montigny, Celine Bourcier de Carbon, Diana Kirilovsky
Article
Biochemistry & Molecular Biology
Qiong Xia, Marine Saux, Maharajah Ponnaiah, Francoise Gilard, Francois Perreau, Stephanie Huguet, Sandrine Balzergue, Nicolas Langlade, Christophe Bailly, Patrice Meimoun, Francoise Corbineau, Hayat El-Maarouf-Bouteau
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Plant Sciences
Marc Galland, Stephanie Boutet-Mercey, Imen Lounifi, Beatrice Godin, Sandrine Balzergue, Olivier Grandjean, Halima Morin, Francois Perreau, Isabelle Debeaujon, Loic Rajjou
PLANT AND CELL PHYSIOLOGY
(2014)
Article
Plant Sciences
Frederic Chauffour, Marlene Bailly, Francois Perreau, Gwendal Cueff, Hiromi Suzuki, Boris Collet, Anne Frey, Gilles Clement, Ludivine Soubigou-Taconnat, Thierry Balliau, Anja Krieger-Liszkay, Loic Rajjou, Annie Marion-Poll
Article
Plant Sciences
Laura Ravazzolo, Sara Trevisan, Alessandro Manoli, Stephanie Boutet-Mercey, Francois Perreau, Silvia Quaggiotti
PLANT AND CELL PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Marina Thierry, Amel Majira, Bruce Pegot, Laurent Cezard, Flavien Bourdreux, Gilles Clement, Francois Perreau, Stephanie Boutet-Mercey, Patrick Diter, Giang Vo-Thanh, Catherine Lapierre, Paul-Henri Ducrot, Emmanuel Magnier, Stephanie Baumberger, Betty Cottyn
