Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Microbiology
Gyu Ri Kim, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Young Ree Kim, Namhee Ryoo, Jong Hee Shin, Jeong Hwan Shin
Summary: This study evaluated the usefulness of MALDI-TOF VITEK MS for Salmonella identification. The results showed that VITEK MS could accurately identify most Salmonella species, but had lower accuracy in identifying typhoidal Salmonella and Salmonella enterica ssp. diarizonae isolates.
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Review
Chemistry, Analytical
Judy Gopal, Manikandan Muthu
Summary: Mushrooms have transitioned from a luxury food to a widely accepted nutrient-rich food. Mushroom polysaccharides have become icons of mushroom bioactivity, and the analytical techniques for their analysis have been consolidated and summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Karl Dichtl, Isabel Klugherz, Hanna Greimel, Josefa Luxner, Julian Koeberl, Simone Friedl, Ivo Steinmetz, Eva Leitner
Summary: This study compared the performance of MALDI Biotyper, EXS2600, and Vitek MS, three commonly used MALDI-TOF MS systems, in testing difficult-to-identify bacteria. The results showed high valid result rates and agreement with sequencing data for all three systems, and low misidentification rates for the majority of isolates.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Applied
Qiuxia Yang, Yushan Guo, Yueming Jiang, Bao Yang
Summary: Oligosaccharides are recognized as prebiotics and their structure is closely related to bioactivity. In this study, UPLC-MS/MS was developed to identify the structure of oligosaccharides, which mainly appeared as sodium adduct in the chromatogram. The fragmentation patterns of oligosaccharides were summarized for different glycosidic linkages. The results showed that UPLC-MS/MS was effective in identifying the structure of oligosaccharides and could be applied in polysaccharide structure identification.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Zhisong Wang, Yuxuan Zhao, Di Wang, Xianglong Zhang, Menglei Xia, Ting Xia, Yu Zheng, Min Wang
Summary: To investigate the formation of typical melanoidin polymers, methylglyoxal (MGO) was reacted with NH3 or alanine (Ala) to form coloured compounds. Control compounds were formed using glyoxal or acetone. The characterisation of the products was done using chromatography, mass spectrometry, and spectroscopy. The results showed that the coloured compounds formed were similar to melanoidins in food, and the MGO-based reaction generated similar volatile compounds using the Maillard reaction. The molecular weights of the structural units in the polymers were mainly 162, 169, and 176 Da, which could be reassembled using basic units derived from MGO or in combination with nitrogen. Therefore, polymers recombined using basic structural units should be considered when determining melanoidin biomarkers. The preparation of coloured compounds using MGO with NH3 can be used as a novel method for producing control compounds for melanoidin after process optimization.
Article
Polymer Science
Molly E. Payne, Oluwapelumi O. Kareem, Kayla Williams-Pavlantos, Chrys Wesdemiotis, Scott M. Grayson
Summary: Poly(ethylene glycol) (PEG) is a cost-effective and commercially available polymer with numerous biomedical and material applications. Despite the incomplete understanding of its oxidative degradation products, the end groups and degradation mechanism of PEG can be proposed and examined using various techniques. Monodisperse PEG allows for the identification of degradation behavior compared to narrowly dispersed PEG, showcasing the potential of MALDI-TOF MS, ESI MS2, and selective functionalization reactions in elucidating polymer end groups and degradation mechanisms.
POLYMER DEGRADATION AND STABILITY
(2021)
Article
Biochemistry & Molecular Biology
Pushpanjali Dasauni, Nirpendra Singh, Varun Chhabra, Manoranjan Mahapatra, Renu Saxena, Suman Kundu
Summary: A method using TFE and heat denaturation prior to digestion was developed and optimized for improved sequence coverage and analysis of Hb variants by nano LC-MALDI MS/MS. The TFE method provided the most suitable efficiency for Hb variant identification based on high sequence coverage.
Article
Biology
Marcelo de M. A. Coura, Eder A. Barbosa, Guilherme D. Brand, Carlos Bloch, Joao B. de Sousa
Summary: The incidence of colorectal cancer (CRC) is increasing in Brazil and there is a lack of reliable biomarkers for diagnosis and prognosis. Alterations in N-glycosylation profiles in serum and tissues of colon cancer patients were identified using mass spectrometry technologies. While serum N-glycans were mostly decreased, certain forms were overexpressed in tumor tissues. There was little correlation between tissue and serum N-glycosylation profiles in cancer patients. Total serum N-glycome analysis showed potential in discriminating patients from healthy controls, but compositions in serum did not parallel those found in tumor tissues.
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Article
Polymer Science
Steffen M. Weidner, Andreas Meyer, Hans R. Kricheldorf
Summary: Alcohol-initiated ROPs of L-Lactide at high temperature result in the formation of cyclic and linear PLA. The crystalline structure of cyclic PLA is made up by extended rings, which is different from that of linear PLA. Experimental results further support this interpretation.
Article
Polymer Science
Hans R. Kricheldorf, Steffen M. Weidner
Summary: In this study, HMN or BUND was used as initiators, and SnOct2 was used as the catalyst for the ROPs of L-Lactide. The results showed that when the reaction was carried out in bulk, only linear chains were obtained, while in solution, mixtures of linear and cyclic poly(L-lactide) were obtained, which is different from previous reports. The mass spectra of the cyclic PLAs displayed a saw-tooth pattern after annealing, indicating that cyclic and linear PLAs crystallized separately from the same reaction mixture. Fractionated crystallization from the solution confirmed that cyclic PLAs nucleate and crystallize faster than linear chains.
Article
Polymer Science
Angelica Velazquez, Daniel Grande, Luis Ernesto Elizalde
Summary: In this study, esters of 2-bromo-2-methylpropanoate of poly(oxyalkylene) polymers were prepared and characterized, with a focus on the effects of solvents and cationic agents on mass spectrometry results. It was found that the analyte undergoes transformation to different end-groups when certain cationic agents are used. The well-defined bromine-terminated macromolecules obtained could serve as ATRP macroinitiators for the synthesis of diverse polymeric architectures for drug delivery bioconjugates.
