Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Patcharapan Suwannin, Duangporn Polpanich, Saovanee Leelayoova, Mathirut Mungthin, Pramuan Tangboriboonrat, Abdelhamid Elaissari, Kulachart Jangpatarapongsa, Toon Ruang-areerate, Tienrat Tangchaikeeree
Summary: The study developed a simple and highly sensitive detection method using LAMP technology and SA-GNPs for visual detection of the 18S rRNA gene of Plasmodium falciparum. This method can complete all steps within 50 minutes, with high specificity and sensitivity, suitable for malaria diagnostics in resource-limited regions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Chemistry, Analytical
Yao Fu, Li Zhang, Yu Xing, Shixiong Deng
Summary: A real-time detection method for DNA methylation was developed in this study, which provides an option for analyzing methylation levels in resource-limited environments and holds great potential for the diagnosis of methylation-related diseases.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Jong-Won Kim, Kyoung-Woo Park, Myeongkun Kim, Kyung Kwan Lee, Chang-Soo Lee
Summary: Loop-mediated isothermal amplification (LAMP) technology offers rapid results, but can lead to false positive outcomes. This study presents a nanomaterial-assisted LAMP method using a graphene oxide-gold nanoparticles (AuNPs@GO) nanocomposite, which shows high sensitivity and specificity for the detection of foot-and-mouth disease virus (FMDV). The approach effectively reduces non-specific signals and may serve as a promising additive in the LAMP system for highly sensitive and specific disease detection, including FMD.
Article
Chemistry, Physical
Jianping Liang, Jie Zeng, Xiaojuan Huang, Tengteng Zhu, Yonglong Gong, Chen Dong, Xiangrong Wang, Lingzhi Zhao, Lei Xie, Kang Liang, Qiongxiang Tan, Yali Cui, Biao Kong, Wenli Hui
Summary: With the increasing global threat of diseases and infections, the development of a fast, low-cost, and easy-to-use point-of-care testing system is essential. This study proposes a rapid visual detection method that integrates loop-mediated isothermal amplification and lateral flow assay for various analytes. The method does not require DNA extraction and prevents false positive results. The whole detection process can be completed within 25 minutes, and the accuracy is measured using the polymorphisms of methylenetetrahydrofolate reductase.
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Maurice A. Matthew, Jevan Christie, Nawu Yang, Chaoqun Yao
Summary: Trichomonas tenax, a protozoan found in the oral cavity, can cause poor oral hygiene and periodontal disease. A newly developed LAMP assay was 1000 times more sensitive than conventional PCR, with high specificity and the ability to provide a fast, cheap, and reliable diagnostic tool for T. tenax. This assay has potential applications in medical and veterinary clinics.
Article
Chemistry, Multidisciplinary
Rajamanickam Sivakumar, So Yeon Park, Nae Yoon Lee
Summary: Here, a quercetin-mediated silver nanoparticle (AgNP) synthesis combined with loop-mediated isothermal amplification (LAMP) technique was used to detect SARS-CoV-2 and Enterococcus faecium using a foldable PMMA microdevice. The LAMP amplicons formed a complex with Ag+ ions, while the quercetin acted as a reducing agent and chelated with Ag+ ions, resulting in the formation of brown-colored AgNPs within 5 minutes. The method exhibited higher sensitivity than agarose gel electrophoresis and had a detection limit of 101 copies μL-1 for SARS-CoV-2 RNA and 101 CFU mL-1 for E. faecium.
Article
Public, Environmental & Occupational Health
Xiaolan Huang, Fei Xiao, Nan Jia, Chunrong Sun, Jin Fu, Zheng Xu, Xiaodai Cui, Hui Huang, Dong Qu, Juan Zhou, Yi Wang
Summary: The ongoing outbreak of monkeypox, caused by monkeypox virus (MPXV), requires a rapid and sensitive MPXV detection method. In this study, a diagnostic test called MPX-LAMP-LFB was developed based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the diagnosis of MPXV infection. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens and has a low sensitivity of 5 copies/µl of plasmid template and 12.5 copies/µl of pseudovirus in human blood samples. The entire process of the assay can be completed in about 1 hour, making it a useful tool for rapid and reliable diagnosis of MPXV infection.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Chemistry, Analytical
Gna Ahn, Se Hee Lee, Min-Suk Song, Beom-Ku Han, Yang-Hoon Kim, Ji-Young Ahn
Summary: Nucleic acid amplification tests are powerful tools for detecting Japanese encephalitis virus, with high sensitivity and specificity. The colorimetric reverse-transcription loop-mediated isothermal amplification technique allows rapid and accurate detection of JEV in 30 minutes, showing its potential for widespread clinical application.
Article
Infectious Diseases
Anthony Ablordey, Evans Ahotor, Charles A. Narh, Sandra A. King, Isra Cruz, Joseph M. Ndung'u, Dziedzom K. de Souza
Summary: Early diagnosis and treatment of Buruli ulcer is crucial to avoid the worsening effects of the disease. The development of new diagnostic tools like the loop-mediated isothermal amplification is encouraged. The study found that using LAMP method with primers targeting IS2404 for Buruli ulcer diagnosis has a good sensitivity and specificity, with the BURULI primer set yielding the best results.
BMC INFECTIOUS DISEASES
(2021)
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Xinggui Yang, Yue Wang, Ying Liu, Junfei Huang, Qinqin Tan, Xia Ying, Yong Hu, Shijun Li
Summary: Brucella abortus is a major pathogen causing abortion in cattle, but current diagnostic methods are limited. A novel detection method combining LAMP and LFIA technologies was developed, demonstrating rapid, sensitive, and specific detection capabilities, suitable for both basic laboratories and field applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)