期刊
ANALYTICA CHIMICA ACTA
卷 814, 期 -, 页码 31-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.01.041
关键词
Cancer biomarkers; HER-3; Anti-HER-3; Single frequency impedance; Biosensor
资金
- TUBITAK (The Scientific and Technological Research Council of Turkey) [109T 172]
We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.
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