☆ 4.7 Article

Exploring metabolic syndrome serum profiling based on gas chromatography mass spectrometry and random forest models

ANALYTICA CHIMICA ACTA (2014)

期刊

ANALYTICA CHIMICA ACTA

卷 827, 期 -, 页码 22-27

出版社

ELSEVIER

DOI: 10.1016/j.aca.2014.04.008

关键词

Metabolic syndrome; Serum profiling; GC-MS; Random forest; Biomarker

类别

Chemistry, Analytical

资金

National Nature Foundation Committee of PR China [21175157, 21105129, 21375151]
China Hunan Provincial Science and Technology Department [2012FJ4139]
Central South University for Special Support of the Basic Scientific Research Project [2010QZZD007]
China Postdoctoral Science Foundation [201104511]

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Protocol

Reagent

摘要

Metabolic syndrome (MetS) is a constellation of the most dangerous heart attack risk factors: diabetes and raised fasting plasma glucose, abdominal obesity, high cholesterol and high blood pressure. Analysis and representation of the variances of metabolic profiles is urgently needed for early diagnosis and treatment of MetS. In current study, we proposed a metabolomics approach for analyzing MetS based on GC-MS profiling and random forest models. The serum samples from healthy controls and MetS patients were characterized by GC-MS. Then, random forest (RF) models were used to visually discriminate the serum changes in MetS based on these GC-MS profiles. Simultaneously, some informative metabolites or potential biomarkers were successfully discovered by means of variable importance ranking in random forest models. The metabolites such as 2-hydroxybutyric acid, inositol and D-glucose, were defined as potential biomarkers to diagnose the MetS. These results obtained by proposed method showed that the combining GC-MS profiling with random forest models was a useful approach to analyze metabolites variances and further screen the potential biomarkers for MetS diagnosis. (C) 2014 Elsevier B.V. All rights reserved.

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