4.7 Article

Graphene-gated biochip for the detection of cardiac marker Troponin I

期刊

ANALYTICA CHIMICA ACTA
卷 809, 期 -, 页码 148-154

出版社

ELSEVIER
DOI: 10.1016/j.aca.2013.11.047

关键词

Lithium ion intercalation; Graphene sheets; Biochip; Troponin I

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  1. CSIR

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We report lithium ion intercalation mediated efficient exfoliation of graphite to form monolithic graphene sheets which have subsequently been investigated for the development of highly sensitive label-free electrochemical detection platform for cardiac biomarker, Troponin I (cTnI). The spectroscopic and morphological analysis demonstrated the formation of defect free graphene sheets which were successfully employed to fabricate an inter-digited microdevice in a drain-source configuration on a silicon biochip. The graphene gated biochip functionalized with anti-cTnI antibodies used in label free detection of cTnI which exhibited an excellent sensitivity in the picogram range (similar to 1 pg mL(-1)) for cTnI without the use of any enzymatic amplification that promises its potential applicability for bio-molecular detection in clinical diagnosis. (C) 2013 Elsevier B.V. All rights reserved.

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