期刊
ANALYTICA CHIMICA ACTA
卷 820, 期 -, 页码 32-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.03.005
关键词
Cephalosporins; Sulfonamides; Tetracyclines; Magnetic beads; Milk; Screen-printed electrodes
资金
- Spanish Ministerio de Ciencia e Innovacion Research Project [CTQ2012-34238]
- Comunidad de Madrid [S2009PPQ-1642]
- Spanish Ministry of Education
Disposable amperometric magnetosensors, involving a mixture of modified-magnetic beads (MBs), for the multiplex screening of cephalosporins (CPHs), sulfonamides (SAs) and tetracyclines (TCs) antibiotic residues in milk are reported for the first time in this work. The multiplexed detection relies on the use of a mixture of target specific modified magnetic beads (MBs) and application of direct competitive assays using horseradish peroxidase (HRP)-labeled tracers. The amperometric responses measured at -0.20 V vs. the Ag pseudo-reference electrode of screen-printed carbon electrodes (SPCE) upon the addition of H2O2 in the presence of hydroquinone (HQ) as redox mediator, were used to monitor the extent of the different affinity reactions. The developed methodology, involving a simple and short pretreatment, allowed discrimination between no contaminated UHT and raw milk samples and samples containing antibiotic residues at the maximum residue limits (MRIs). The usefulness of the multiplexed magnetosensor was demonstrated by analyzing spiked milk samples in only 5 min. The results demonstrated that a clear discrimination of milk samples contaminated with antibiotics at their MRL level or their mixtures, allowing the identification of milk not complying with current legislation. These features make the developed methodology a promising alternative in the development of user-friendly devices for on-site analysis to ensure quality control for dairy products. (C) 2014 Elsevier B.V. All rights reserved.
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