期刊
ANALYTICA CHIMICA ACTA
卷 808, 期 -, 页码 83-93出版社
ELSEVIER
DOI: 10.1016/j.aca.2013.07.026
关键词
Reverse-phase chromatography; Ion mobility separation; Tandem mass spectrometry; Polysorbate 85; Nonionic surfactants; Isobar differentiation
资金
- National Science Foundation [CHE-1012636]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1012636] Funding Source: National Science Foundation
Liquid chromatography (LC) and ion mobility (IM) separation have been coupled with mass spectrometry (MS) and tandem mass spectrometry (MS2) to characterize a commercially important nonionic surfactant, polysorbate 85. The constituents of this amphiphilic blend contained a sorbitan or isosorbide core that was chain extended with poly(ethylene oxide) (PEO) and partially esterified at the PEO termini with oleic acid or, to a lesser extent, other fatty acids. Using interactive LC in reverse-phase mode, the oligomers of the surfactant were separated according to their hydrophobicity/hydrophilicity balance. On the other hand, IM spectrometry dispersed the surfactant oligomers by their charge and collision cross section (i.e. size/shape). With either separation method, an increased number of fatty ester groups and/or lack of the polar sorbitan (or isosorbide) core led to higher retention/drift times, enabling the separation of isobaric species or species with superimposed isotope patterns, so that their ester content could be conclusively identified by MS2. LC-MS and IM-MS permitted the detection of several byproducts besides the major PEO-sorbitan oleate oligomers. LC-MS provides the separation resolution needed for quantitative determination of the degree of esterification. IM-MS, which minimizes analysis time and solvent use, is ideally suitable for a fast, qualitative survey of samples differing in their minor constituents or impurities. (C) 2013 Elsevier B. V. All rights reserved.
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