Article
Agronomy
Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Xiaohong Yan, Ping Shen, Hongfei Gao, Gang Wu
Summary: An unmarked electrochemical impedance genosensor using gold carbon dots has been developed for the detection of genetically modified crops. This sensor is portable and easy to use, making it suitable for resource-limited laboratories.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biotechnology & Applied Microbiology
Suresh Reddy Banda, Holger Klapproth, Nicolaas Smit, Sonja Bednar, Thomas Brandstetter, Juergen Ruehe
Summary: The sensitivity of a PCR-based biochip assay depends on the efficiency of PCR amplicons in binding to the microarray spots. Asymmetric PCR is often inefficient in generating single-stranded (ss) amplicons. However, a novel variant called Asymmetric Exponential and Linear Amplification (AELA) can overcome these issues and generate large amounts of ss-amplicons. AELA-PCR combines both exponential and linear amplification of the target nucleic acid, resulting in higher biochip sensitivity compared to conventional methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kamila Malecka, Balwinder Kaur, D. Andrea Cristaldi, Clarissa S. Chay, Iwona Mames, Hanna Radecka, Jerzy Radecki, Eugen Stulz
Summary: A cobalt-porphyrin modified DNA was used as an electrochemical marker to detect specific DNA sequences, comparing the performance of gold and silver nanoparticles in oligonucleotide sensors. Surprisingly, both types of sensors showed similar overall performance, with direct electron transfer between the porphyrin and the electrode. The silver system displayed better DNA economy and cost-effectiveness compared to gold, making it more suitable for mass production of highly sensitive DNA sensors.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Ece Eksin
Summary: A new genosensor was developed for sensitive and selective detection of the gene sequence of Acinetobacter baumannii. It utilizes a capture probe immobilized on chitosan modified single-use pencil graphite electrodes (c-PGEs) to recognize the target DNA sequence. The genosensor showed a low limit of detection and no interference from non-complementary sequences.
Article
Chemistry, Analytical
Mojtaba Shamsipur, Leila Samandari, Leila Farzin, Abbas Besharati-Seidani
Summary: In this study, a simple and label-free voltammetric genosensor based on lead ion-imprinted polymer nanoparticles was developed for the determination of the HIV-1 pole gene. The modified electrode showed a well-defined lead oxidation peak with excellent stability and reproducibility. Under optimized conditions, the genosensor exhibited a linear detection range from 1 fM to 0.1 nM with a detection limit of 0.3 fM (S/N = 3).
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
D. Ogonczyk, P. Jankowski
Summary: In this paper, the authors present a method for fabricating gold-plated polycarbonate microchannels by adjusting the sequence of reagent insertion. The resulting gold surface can be used in various applications that combine the advantages of microfluidics, surface chemistry, and electrochemistry. The authors demonstrate the combination of electrochemistry and microfluidics by integrating a gold sensor into the microfluidic system.
Article
Chemistry, Analytical
Rachel Rui Xia Lim, Wei Li Ang, Adriano Ambrosi, Zdenek Sofer, Alessandra Bonanni
Summary: Electrochemical polymerase chain reaction (PCR) is a valid alternative to optical-based PCR, with advantages such as reduced costs, simpler instrumentation, and miniaturization and portability of systems. This study compared three different nanographene oxide materials as signaling tags for the detection of genetically modified organisms (GMO) and identified the nanocarbon material with the highest potential for use as an electroactive label in PCR amplification and detection.
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Chemistry, Analytical
Elena Suprun, Svetlana A. Khmeleva, Konstantin Bibik, Konstantin G. Ptitsyn, Leonid K. Kurbatov, Sergey P. Radko
Summary: In this study, modified dUTP-Fl and dUTP-Rh were used as substrates and electroactive labels for electrochemical detection of dsDNA amplification products. The results showed that dUTP-Fl had better compatibility with PCR and RPA, allowing the direct electrochemical detection of full-size amplicons at micromolar concentrations when substituted in the reaction mixture at 50-90% of dTTP.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Luis Fernando Garcia-Melo, Miguel Morales-Rodriguez, Eduardo Madrigal-Bujaidar, Eduardo O. Madrigal-Santillan, Jose Antonio Morales-Gonzalez, Rosa Natali Pineda Cruces, Jorge Alfredo Campoy Ramirez, Pablo Damian-Matsumura, Alexandro Tellez-Plancarte, Nikola Batina, Isela Alvarez-Gonzalez
Summary: In this study, an electrochemical genosensor was constructed for the identification of diseases related to the K-ras oncogene. The genosensor utilized SPGE, CV, and AFM techniques to monitor electron transfer and morphological changes on a functionalized nanostructured surface. The genosensor demonstrated a linear response for the identification of the k12p.1 mutation of the K-ras gene, with a low detection limit, in the presence of Dox as a DNA intercalating agent.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ruidi Shen, Ji Zhang, Wenxiu Huang, Shaoyong Wu, Gongke Li, Seyin Zou, Liansheng Ling
Summary: The study developed a new diagnostic method using specific oligonucleotides to detect CA-125 through PCR and AuNPs-DNA probes, achieving rapid diagnosis of ovarian cancer.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Sudheendran Mavila, Heidi R. Culver, Alex J. Anderson, Tania R. Prieto, Christopher N. Bowman
Summary: An athermal approach using self-immolative thioester linked nucleic acids (TENA) for mRNA enrichment from total RNA has shown comparable performance to a commercial kit. The method is compatible with downstream applications such as RT-qPCR for gene expression analysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Cristina Munoz-San Martin, Ana Montero-Calle, Maria Garranzo-Asensio, Maria Gamella, Victor Perez-Gines, Maria Pedrero, Jose M. Pingarron, Rodrigo Barderas, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Susana Campuzano
Summary: This work presents the first bioelectronic immunoplatforms for determining glycosylated haptoglobin (Hp) and both total and glycosylated Hp simultaneously. The immunoplatforms use non-competitive bioassays and magnetic microcarriers labeled with horseradish peroxidase (HRP) for detection. The method allows accurate determination of total and glycosylated Hp in colorectal cancer (CRC) cells, which is not possible with commercial ELISA kits and Western blot methodology due to lack of sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Lourdes Agui, Araceli Gonzalez-Cortes, Susana Campuzano, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: The study of the human microbiome is a multidisciplinary field that holds great potential for improving disease diagnosis and monitoring using microbiota biomarkers. Electrochemical biosensors, with their high sensitivity and fast response, combined with advancements in nanomaterials, offer unique tools for understanding the human microbiome and driving future developments. This review article focuses on the application of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers in the gastrointestinal microbiome, discussing challenges and future directions.
Article
Chemistry, Analytical
Victor Perez-Gines, Rebeca M. Torrente-Rodriguez, Maria Pedrero, Neus Martinez-Bosch, Pablo Garcia de Frutos, Pilar Navarro, Jose M. Pingarron, Susana Campuzano
Summary: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in developed countries and predicted to be the second leading cause in 2030. A disposable amperometric immunoplatform for the determination of CA19-9 has been developed in this study. The immunoplatform combines magnetic microsupports (MBs) for immunoassay implementation and amperometric transduction on screen-printed carbon electrodes (SPCEs). The immunoplatform showed good reproducibility, selectivity, and storage stability, and was able to differentiate healthy subjects from PDAC patients within 1 hour. It represents an improvement in terms of cost, applicability, and accessibility compared to current ELISA-based techniques.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susana Campuzano, Maria Gamella, Maria Pedrero, Jose M. Pingarron
Summary: The role of liquid biopsy in precision, equitable, and sustainable diagnosis of prevalent and highly heterogeneous diseases using biomarkers derived from whole cells, secretomes, and exosomes is undeniable. Electrochemical biosensing and biosensors, particularly those involving affinity reactions, have emerged as competitive and cost-effective analytical alternatives for identifying these challenging targets. This review provides a concise and up-to-date overview of the fundamentals, versatility, and opportunities offered by state-of-the-art electrochemical biosensing (2018-2022) for the determination of cellular-derived biomarkers. It also offers critical insights into the short-term challenges and future research directions in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Ana Montero-Calle, Maria Garranzo-Asensio, Rebeca M. Torrente-Rodriguez, Victor Ruiz-Valdepenas Montiel, Carmen Poves, Jana Dziakova, Rodrigo Sanz, Cristina Diaz del Arco, Jose Manuel Pingarron, Maria Jesus Fernandez-Acenero, Susana Campuzano, Rodrigo Barderas
Summary: The study aimed to assess whether p53 and p63 proteoforms derived from alternative splicing could have a differential seroreactivity and higher diagnostic value than canonical proteins in colorectal cancer. The researchers demonstrated the appearance of a differential seroreactivity among the proteoforms in colorectal cancer patients. These findings reveal increased complexity of the humoral immune response in cancer and highlight the potential for developing a blood-based biomarker signature for colorectal cancer detection.
Article
Chemistry, Analytical
Ana M. Villa-Manso, Tamara Guerrero-Esteban, Felix Pariente, Celia Toyos-Rodriguez, Alfredo de la Escosura-Muniz, Monica Revenga-Parra, Cristina Gutierrez-Sanchez, Encarnacion Lorenzeo
Summary: A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) has been developed for the sensitive determination of N protein of the SARS-CoV-2 coronavirus. The immunosensor utilizes bifunctional core@shell nanoparticles as ECL acceptor and [Ru(bpy)(3)](2+) as ECL donor. It demonstrates a wide linear response to the concentration of N protein with a detection limit of 1.27 pg/mL, high stability, and specificity. The immunosensor has been successfully applied for quantification of N protein in saliva samples and showed consistent results with a commercial colorimetric ELISA kit.
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: Natural enzymes are used as special reagents in the preparation of electrochemical (bio)sensors, but their practical applications are limited due to certain drawbacks. Artificial enzymes based on nanomaterials, known as nanozymes, have been developed as an alternative, providing enhanced stability, low cost, easy production, and rapid activity. Among nanozymes, carbon-based nanozymes have been widely used in the preparation of electrochemical (bio)sensors due to their stable activity, tunability, and good biocompatibility.
Article
Chemistry, Multidisciplinary
Ponnusamy Nandhakumar, Cristina Munoz San Martin, Beatriz Arevalo, Shichao Ding, Mahika Lunker, Eva Vargas, Omeed Djassemi, Susana Campuzano, Joseph Wang
Summary: In order to address the low sensitivity issue of conventional LFA, we report a simple electrochemical LFA with nanocatalytic redox cycling for decentralized insulin detection. This method achieves higher sensitivity than traditional methods by amplifying the signal through nanoparticles and chemical reactions.
Review
Chemistry, Multidisciplinary
Susana Campuzano, Jose M. Pingarron
Summary: Electrochemical affinity biosensors are rapidly evolving and becoming increasingly important in our daily lives. They combine their intrinsic attributes with advances in technology to offer a wide range of opportunities and applications. This perspective provides an updated and critical view of these biosensors and discusses the challenges and future directions they face.
Review
Chemistry, Physical
Susana Campuzano, Jose M. Pingarron
Summary: The recent advances in electrochemical biosensing have demonstrated the versatility and adaptability of biotools. Innovations in bioreceptor production, electrode substrate fabrication, miniaturizable electronics, materials science, digitization, and artificial intelligence have also contributed to the evolution of electrochemical biosensing.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Karim Kaci, Estefania Enebral-Romero, Emiliano Martinez-Perinan, Marina Garrido, Emilio M. Perez, David Lopez-Diego, Monica Luna, Guillermo Gonzalez-de-Rivera, Tania Garcia-Mendiola, Encarnacion Lorenzo
Summary: An advanced, cost-effective, and portable DNA biosensor capable of detecting multiple bacteria simultaneously has been developed. The device is compact, lightweight, and manufactured at a low cost. The results of this study confirm its reliability and superior performance.
Article
Biochemistry & Molecular Biology
Sandra Tejerina-Miranda, Maria Pedrero, Marina Blazquez-Garcia, Veronica Serafin, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, Jose M. Pingarron, Rodrigo Barderas, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for determining human endostatin, which shows high sensitivity and selectivity for diagnosing tumors and identifying patients in different stages.
BIOELECTROCHEMISTRY
(2024)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
