期刊
ANALYTICA CHIMICA ACTA
卷 753, 期 -, 页码 8-18出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.09.041
关键词
Organelle isolation; Organelle purification; Subcellular; Centrifugation; Electrophoresis; Dielectrophoresis; Fluorescence; Atomic force microscopy; Microfluidics
资金
- NIH [R01AG020866]
- Center for Analysis of Biomolecular Signaling at the University of Minnesota-Twin Cities
- Center for Advanced Bioanalytical Technologies [P206/12/G014]
- Czech Science Foundation
- Republic Institutional research plan, Academy of Sciences of the Czech Republic [AV0Z40310501]
The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices. (C) 2012 Elsevier B.V. All rights reserved.
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