Review
Plant Sciences
Aleksandr Ivanov, Irina Safenkova, Anatoly Zherdev, Boris B. Dzantiev
Summary: Rapid, sensitive, and timely diagnostics are crucial for protecting plants from pathogens, with isothermal amplification methods providing a feasible solution for in-field molecular diagnostics. Combining isothermal amplification techniques with extraction and detection methods compatible with outdoor phytodiagnostics is essential for preventing and controlling the spread of disease caused by viral, bacterial, and fungal phytopathogens. The development of rapid, sensitive, and equipment-free nucleic acid detection methods is seen as the future of phytodiagnostics, showcasing visible benefits.
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
You-Ru Jhou, Chih-Hung Wang, Huey-Pin Tsai, Yan-Shen Shan, Gwo-Bin Lee
Summary: An integrated microfluidic platform utilizing real-time reverse-transcription loop-mediated isothermal amplification was developed for detection and quantification of three genes of SARS-CoV-2, providing a useful tool for COVID-19 diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Beatriz Jorge Coelho, Bruno Veigas, Luis Bettencourt, Hugo Aguas, Elvira Fortunato, Rodrigo Martins, Pedro Baptista, Rui Igreja
Summary: We introduce a digital microfluidics (DMF) platform for loop-mediated isothermal amplification (LAMP) of DNA, and apply it to real-time amplification for monitoring c-Myc, a cancer biomarker associated with 40% of human tumors. We demonstrate successful manipulation of samples and reagents on the DMF platform, achieving amplification of 90 pg of target DNA in less than one hour. Innovative mixing strategies in DMF, including low frequency AC actuation and back-and-forth droplet motion, improve fluorescence readouts. On-chip sample partitioning and sequential droplet irradiation minimize fluorophore bleaching effects.
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Daehan Nam, Seokjoon Kim, Jung Ho Kim, Seungjin Lee, Daneub Kim, Jinseo Son, Doyeon Kim, Byung Seok Cha, Eun Sung Lee, Ki Soo Park
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used molecular diagnostics technology. This study introduces a new version of LAMP, called low-temperature LAMP, which operates at 37 degrees C for on-site diagnosis. The low-temperature LAMP differs from conventional LAMP in terms of MgSO4 and dNTPs concentrations, as well as the lengths of DNA probes. Under optimal conditions, low-temperature LAMP shows comparable amplification efficiency to conventional LAMP. Additionally, the method enables the detection of miR-21 with high sensitivity and selectivity at 37 degrees C, without requiring expensive DNA modifications or special additives.
Article
Chemistry, Multidisciplinary
Jacob Amos Hambalek, Janay Elise Kong, Calvin Brown, Hector Enrique Munoz, Thomas Horn, Michael Bogumil, Eleni Quick, Aydogan Ozcan, Dino Di Carlo
Summary: The study demonstrates a simple workflow for detecting methylated CpG dinucleotides in DNA samples using restriction enzyme digestion and loop-mediated isothermal amplification. Additionally, a cost-effective portable fluorescence reader is shown to transduce fluorescence signals associated with DNA amplification.
Article
Biotechnology & Applied Microbiology
Hyeonseek Park, Doo Ri Kim, Areum Shin, Eunjung Jeong, Sohee Son, Jin-Hyun Ahn, So Yoon Ahn, Suk-Joo Choi, Soo-young Oh, Yun Sil Chang, Yae-Jean Kim, Minhee Kang
Summary: Congenital CMV infection is a common cause of hearing loss and neurodevelopmental impairment in newborns. A robust LAMP assay was developed for large-scale screening of newborns, providing higher sensitivity and shorter detection time compared to qPCR. The colorimetric LAMP assay can also be used for point-of-care testing in low-resource environments.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Guangcheng Luo, Tingting Yi, Qiang Wang, Bin Guo, Li Fang, Guoyuan Zhang, Xiaolan Guo
Summary: This study introduces a novel nucleic acid detection method called SPA, which amplifies target nucleic acids specifically and sensitively using Bst DNA polymerase and stem-loop primers. Compared to traditional PCR and LAMP technologies, SPA is more specific and convenient. The pH indicator-based colorimetric SPA showed 100% accuracy for HPV16 detection in cervical samples, indicating its great potential for POCT nucleic acid testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Jorn Gloekler, Theam Soon Lim, Jeunice Ida, Marcus Frohme
Summary: The introduction of nucleic acid amplification techniques, particularly isothermal amplification methods, has revolutionized the field of medical diagnostics by simplifying nucleic acid amplification and impacting the design of molecular diagnostics. Despite the advantages they bring, the heterogeneous issues and complexities surrounding different isothermal amplification methods make it challenging to identify the best approach.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Agriculture, Dairy & Animal Science
Alexandra Knox, Travis Beddoe
Summary: Equine viral diseases pose a significant threat to global health, with some viruses having zoonotic potential. Current diagnostic techniques are time-consuming, costly, and require laboratory conditions. New isothermal nucleic acid amplification technologies, such as LAMP and iiPCR, offer rapid results within an hour and potential for field deployment.
Article
Chemistry, Multidisciplinary
Ali Amadeh, Erfan Ghazimirsaeed, Amir Shamloo, Mahdi Dizani
Summary: Adding TiO2 nanoparticles in a photonic PCR system benefits gene amplification by improving heating and cooling rates while reducing energy consumption. The system demonstrates high temperature stability and accuracy, capable of amplifying eight gene samples simultaneously. The addition of TiO2 nanoparticles enhances thermal conductivity, leading to more efficient gene amplification.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mengyun Zhou, Chuan Fan, Lirong Wang, Tailin Xu, Xueji Zhang
Summary: This study reports an integrated microdroplet array platform composed of an ultrasonic unit and minipillar array for enhanced RPA detection of SARS-CoV-2. The independent microvolume reactions and microstreaming driven by ultrasound greatly accelerate the amplification and increase the sensitivity, while reducing reagent consumption.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Summary: This new isothermal nucleic acid amplification assay, called RICCA, allows for rapid and direct detection of viruses with low viral loads. It has shown high sensitivity in detecting RNA viruses directly from saliva samples and synthetic templates of SARS-CoV-2. The assay can be used in real-time point-of-care testing and has a total assay time of 15-30 minutes for detecting heat-inactivated SARS-CoV-2 virus in human saliva.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Chao Zhang, Tarun Belwal, Zisheng Luo, Bin Su, Xingyu Lin
Summary: This article comprehensively discusses the application of different nanomaterials in isothermal nucleic acid amplification, including the roles of 0D, 1D, 2D, and 3D nanomaterials, as well as recent progress in the field. Nanomaterials are commonly used for reaction enhancement, signal generation/amplification, or surface loading. Additionally, 3D nanomaterials can serve as isolated chambers for digital nucleic acid amplification and DNA sequencing tools, while challenges and recommendations are proposed for future applications.
Article
Biotechnology & Applied Microbiology
Tanja Kostic, Michael Ellis, Maggie R. Williams, Tiffany M. Stedtfeld, John B. Kaneene, Robert D. Stedtfeld, Syed A. Hashsham
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Engineering, Biomedical
Robert D. Stedtfeld, Yen-Cheng Liu, Tiffany M. Stedtfeld, Tanja Kostic, Maggie Kronlein, Onnop Srivannavit, Walid T. Khalife, James M. Tiedje, Erdogan Gulari, Mary Hughes, Brett Etchebarne, Syed A. Hashsham
BIOMEDICAL MICRODEVICES
(2015)
Article
Biochemical Research Methods
Gregoire Seyrig, Robert D. Stedtfeld, Dieter M. Tourlousse, Farhan Ahmad, Keara Towery, Alison M. Cupples, James M. Tiedje, Syed A. Hashsham
JOURNAL OF MICROBIOLOGICAL METHODS
(2015)
Article
Biotechnology & Applied Microbiology
Yogendra H. Kanitkar, Robert D. Stedtfeld, Robert J. Steffan, Syed A. Hashsham, Alison M. Cupples
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Biochemical Research Methods
Robert D. Stedtfeld, Tiffany M. Stedtfeld, Farag Samhan, Yogendra H. Kanitkar, Paul B. Hatzinger, Alison M. Cupples, Syed A. Hashsham
JOURNAL OF MICROBIOLOGICAL METHODS
(2016)
Article
Microbiology
Timothy A. Johnson, Robert D. Stedtfeld, Qiong Wang, James R. Cole, Syed A. Hashsham, Torey Looft, Yong-Guan Zhu, James M. Tiedje
Article
Environmental Sciences
Robert D. Stedtfeld, Tiffany M. Stedtfeld, Hassen Waseem, Meridith Fitschen-Brown, Xueping Guo, Benli Chai, Maggie R. Williams, Trevor Shook, Amanda Logan, Ally Graham, Jong-Chan Chae, Woo-Jun Sul, Jacob VanHouten, James R. Cole, Gerben J. Zylstra, James M. Tiedje, Brad L. Upham, Syed A. Hashsham
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2017)
Article
Biochemical Research Methods
Farhan Ahmad, Robert D. Stedtfeld, Hassan Waseem, Maggie R. Williams, Alison M. Cupples, James M. Tiedje, Syed A. Hashsham
JOURNAL OF MICROBIOLOGICAL METHODS
(2017)
Article
Biochemical Research Methods
Vidya Srinivasan, Robert D. Stedtfeld, Dieter M. Tourlousse, Samuel W. Baushke, Yu Xin, Sarah M. Miller, Trinh Pham, Jean-Marie Rouillard, Erdogan Gulari, James M. Tiedje, Syed A. Hashsham
JOURNAL OF MICROBIOLOGICAL METHODS
(2017)
Article
Engineering, Environmental
Farag A. Samhan, Tiffany M. Stedtfeld, Hassan Waseem, Maggie R. Williams, Robert D. Stedtfeld, Syed A. Hashsham
Article
Multidisciplinary Sciences
Benli Chai, Tamara V. Tsoi, Shoko Iwai, Cun Liu, Jordan A. Fish, Cheng Gu, Timothy A. Johnson, Gerben Zylstra, Brian J. Teppen, Hui Li, Syed A. Hashsham, Stephen A. Boyd, James R. Cole, James M. Tiedje
Article
Engineering, Biomedical
Maggie R. Williams, Robert D. Stedtfeld, Tiffany M. Stedtfeld, James M. Tiedje, Syed A. Hashsham
BIOMEDICAL MICRODEVICES
(2017)
Article
Biotechnology & Applied Microbiology
Yogendra H. Kanitkar, Robert D. Stedtfeld, Paul B. Hatzinger, Syed A. Hashsham, Alison M. Cupples
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Review
Chemistry, Analytical
M. R. Williams, R. D. Stedtfeld, H. Waseem, T. Stedtfeld, B. Upham, W. Khalife, B. Etchebarne, M. Hughes, J. M. Tiedje, S. A. Hashsham
ANALYTICAL METHODS
(2017)
Article
Microbiology
Robert D. Stedtfeld, Maggie R. Williams, Umama Fakher, Timothy A. Johnson, Tiffany M. Stedtfeld, Fang Wang, Walid T. Khalife, Mary Hughes, Brett E. Etchebarne, James M. Tiedje, Syed A. Hashsham
FEMS MICROBIOLOGY ECOLOGY
(2016)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)